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Development of a Murine Model of Early Sepsis in Diet-Induced Obesity
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| Zeitschriftentitel: | BioMed Research International |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | BioMed Research International, 2014, 2014, S. 1-11 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Hindawi Limited
|
| Schlagwörter: |
| author_facet |
Khan, Momina Patrick, Amanda L. Fox-Robichaud, Alison E. Translational Biology Group, The Canadian Critical Care Khan, Momina Patrick, Amanda L. Fox-Robichaud, Alison E. Translational Biology Group, The Canadian Critical Care |
|---|---|
| author |
Khan, Momina Patrick, Amanda L. Fox-Robichaud, Alison E. Translational Biology Group, The Canadian Critical Care |
| spellingShingle |
Khan, Momina Patrick, Amanda L. Fox-Robichaud, Alison E. Translational Biology Group, The Canadian Critical Care BioMed Research International Development of a Murine Model of Early Sepsis in Diet-Induced Obesity General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine |
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khan, momina |
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Khan, Momina Patrick, Amanda L. Fox-Robichaud, Alison E. Translational Biology Group, The Canadian Critical Care 2314-6133 2314-6141 Hindawi Limited General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1155/2014/719853 <jats:p>Sepsis, a global health issue, is the most common cause of mortality in the intensive care unit. The aim of this study was to develop a new model of sepsis that investigates the impact of prolonged western diet (WD) induced obesity on the response to early sepsis. Male C57BL/6 mice were fed either a high fat WD or normal chow diet (NCD) for 6, 15, or 27 weeks. Septic obese mice at 15 and 27 weeks had significantly lower levels of lung myeloperoxidase (26.3 ± 3.80 U/mg tissue) compared to age matched ad lib (44.1 ± 2.86 U/mg tissue) and diet restricted (63.2 ± 5.60 U/mg tissue) controls. Low levels of lung inflammation were not associated with changes in hepatic cytokines and oxidative stress levels. Obese mice had significantly (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn fontstyle="italic">0.0001</mml:mn></mml:math>) larger livers compared to controls. Histological examination of the livers demonstrated that WD fed mice had increased inflammation with pronounced fat infiltration, steatosis, and hepatocyte ballooning. Using this model of prolonged exposure to high fat diet we have data that agree with recent clinical observations suggesting obese individuals are protected from sepsis-induced lung injury. This model will allow us to investigate the links between damage to the hepatic microcirculation, immune response, and lung injury.</jats:p> Development of a Murine Model of Early Sepsis in Diet-Induced Obesity BioMed Research International |
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| title |
Development of a Murine Model of Early Sepsis in Diet-Induced Obesity |
| title_unstemmed |
Development of a Murine Model of Early Sepsis in Diet-Induced Obesity |
| title_full |
Development of a Murine Model of Early Sepsis in Diet-Induced Obesity |
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Development of a Murine Model of Early Sepsis in Diet-Induced Obesity |
| title_full_unstemmed |
Development of a Murine Model of Early Sepsis in Diet-Induced Obesity |
| title_short |
Development of a Murine Model of Early Sepsis in Diet-Induced Obesity |
| title_sort |
development of a murine model of early sepsis in diet-induced obesity |
| topic |
General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine |
| url |
http://dx.doi.org/10.1155/2014/719853 |
| publishDate |
2014 |
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1-11 |
| description |
<jats:p>Sepsis, a global health issue, is the most common cause of mortality in the intensive care unit. The aim of this study was to develop a new model of sepsis that investigates the impact of prolonged western diet (WD) induced obesity on the response to early sepsis. Male C57BL/6 mice were fed either a high fat WD or normal chow diet (NCD) for 6, 15, or 27 weeks. Septic obese mice at 15 and 27 weeks had significantly lower levels of lung myeloperoxidase (26.3 ± 3.80 U/mg tissue) compared to age matched ad lib (44.1 ± 2.86 U/mg tissue) and diet restricted (63.2 ± 5.60 U/mg tissue) controls. Low levels of lung inflammation were not associated with changes in hepatic cytokines and oxidative stress levels. Obese mice had significantly (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn fontstyle="italic">0.0001</mml:mn></mml:math>) larger livers compared to controls. Histological examination of the livers demonstrated that WD fed mice had increased inflammation with pronounced fat infiltration, steatosis, and hepatocyte ballooning. Using this model of prolonged exposure to high fat diet we have data that agree with recent clinical observations suggesting obese individuals are protected from sepsis-induced lung injury. This model will allow us to investigate the links between damage to the hepatic microcirculation, immune response, and lung injury.</jats:p> |
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| author | Khan, Momina, Patrick, Amanda L., Fox-Robichaud, Alison E., Translational Biology Group, The Canadian Critical Care |
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| description | <jats:p>Sepsis, a global health issue, is the most common cause of mortality in the intensive care unit. The aim of this study was to develop a new model of sepsis that investigates the impact of prolonged western diet (WD) induced obesity on the response to early sepsis. Male C57BL/6 mice were fed either a high fat WD or normal chow diet (NCD) for 6, 15, or 27 weeks. Septic obese mice at 15 and 27 weeks had significantly lower levels of lung myeloperoxidase (26.3 ± 3.80 U/mg tissue) compared to age matched ad lib (44.1 ± 2.86 U/mg tissue) and diet restricted (63.2 ± 5.60 U/mg tissue) controls. Low levels of lung inflammation were not associated with changes in hepatic cytokines and oxidative stress levels. Obese mice had significantly (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn fontstyle="italic">0.0001</mml:mn></mml:math>) larger livers compared to controls. Histological examination of the livers demonstrated that WD fed mice had increased inflammation with pronounced fat infiltration, steatosis, and hepatocyte ballooning. Using this model of prolonged exposure to high fat diet we have data that agree with recent clinical observations suggesting obese individuals are protected from sepsis-induced lung injury. This model will allow us to investigate the links between damage to the hepatic microcirculation, immune response, and lung injury.</jats:p> |
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| spelling | Khan, Momina Patrick, Amanda L. Fox-Robichaud, Alison E. Translational Biology Group, The Canadian Critical Care 2314-6133 2314-6141 Hindawi Limited General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1155/2014/719853 <jats:p>Sepsis, a global health issue, is the most common cause of mortality in the intensive care unit. The aim of this study was to develop a new model of sepsis that investigates the impact of prolonged western diet (WD) induced obesity on the response to early sepsis. Male C57BL/6 mice were fed either a high fat WD or normal chow diet (NCD) for 6, 15, or 27 weeks. Septic obese mice at 15 and 27 weeks had significantly lower levels of lung myeloperoxidase (26.3 ± 3.80 U/mg tissue) compared to age matched ad lib (44.1 ± 2.86 U/mg tissue) and diet restricted (63.2 ± 5.60 U/mg tissue) controls. Low levels of lung inflammation were not associated with changes in hepatic cytokines and oxidative stress levels. Obese mice had significantly (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn fontstyle="italic">0.0001</mml:mn></mml:math>) larger livers compared to controls. Histological examination of the livers demonstrated that WD fed mice had increased inflammation with pronounced fat infiltration, steatosis, and hepatocyte ballooning. Using this model of prolonged exposure to high fat diet we have data that agree with recent clinical observations suggesting obese individuals are protected from sepsis-induced lung injury. This model will allow us to investigate the links between damage to the hepatic microcirculation, immune response, and lung injury.</jats:p> Development of a Murine Model of Early Sepsis in Diet-Induced Obesity BioMed Research International |
| spellingShingle | Khan, Momina, Patrick, Amanda L., Fox-Robichaud, Alison E., Translational Biology Group, The Canadian Critical Care, BioMed Research International, Development of a Murine Model of Early Sepsis in Diet-Induced Obesity, General Immunology and Microbiology, General Biochemistry, Genetics and Molecular Biology, General Medicine |
| title | Development of a Murine Model of Early Sepsis in Diet-Induced Obesity |
| title_full | Development of a Murine Model of Early Sepsis in Diet-Induced Obesity |
| title_fullStr | Development of a Murine Model of Early Sepsis in Diet-Induced Obesity |
| title_full_unstemmed | Development of a Murine Model of Early Sepsis in Diet-Induced Obesity |
| title_short | Development of a Murine Model of Early Sepsis in Diet-Induced Obesity |
| title_sort | development of a murine model of early sepsis in diet-induced obesity |
| title_unstemmed | Development of a Murine Model of Early Sepsis in Diet-Induced Obesity |
| topic | General Immunology and Microbiology, General Biochemistry, Genetics and Molecular Biology, General Medicine |
| url | http://dx.doi.org/10.1155/2014/719853 |