Eintrag weiter verarbeiten
Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island”
Gespeichert in:
| Zeitschriftentitel: | Journal of Neurophysiology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Journal of Neurophysiology, 85, 2001, 4, S. 1772-1776 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
|
| Schlagwörter: |
| author_facet |
Johnson, Shereé M. Koshiya, Naohiro Smith, Jeffrey C. Johnson, Shereé M. Koshiya, Naohiro Smith, Jeffrey C. |
|---|---|
| author |
Johnson, Shereé M. Koshiya, Naohiro Smith, Jeffrey C. |
| spellingShingle |
Johnson, Shereé M. Koshiya, Naohiro Smith, Jeffrey C. Journal of Neurophysiology Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” Physiology General Neuroscience |
| author_sort |
johnson, shereé m. |
| spelling |
Johnson, Shereé M. Koshiya, Naohiro Smith, Jeffrey C. 0022-3077 1522-1598 American Physiological Society Physiology General Neuroscience http://dx.doi.org/10.1152/jn.2001.85.4.1772 <jats:p> The pre-Bötzinger complex (pre-BötC), a bilaterally distributed network of rhythmogenic neurons within the ventrolateral medulla, has been proposed to be the critical locus for respiratory rhythm generation in mammals. To date, thin transverse medullary slice preparations that capture the pre-BötC have served as the optimal experimental model to study the region's inherent cellular and network properties. We have reduced the thin slices to isolated pre-BötC “islands” to further establish whether the pre-BötC has intrinsic rhythmicity and is the kernel for rhythmogenesis in the slice. We recorded neuron population activity locally in the pre-BötC with macroelectrodes and fluorescent imaging of Ca<jats:sup>2+</jats:sup> activities with Calcium Green-1AM dye before and after excising the island. The isolated island remained rhythmically active with a population burst profile similar to the inspiratory burst in the slice. Rhythmic population activity persisted in islands after block of GABA<jats:sub>A</jats:sub>ergic and glycinergic synaptic inhibition. The loci of pre-BötC Ca<jats:sup>2+</jats:sup> activity imaged in thin slices and islands were similar, and imaged pre-BötC neurons exhibited synchronized flashing after blocking synaptic inhibition. Population burst frequency increased monotonically as extracellular potassium concentration was elevated, consistent with mathematical models consisting entirely of an excitatory network of synaptically coupled pacemaker neurons with heterogeneous, voltage-dependent bursting properties. Our results provide further evidence for a rhythmogenic kernel in the pre-BötC in vitro and demonstrate that the islands are ideal preparations for studying the kernel's intrinsic properties. </jats:p> Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” Journal of Neurophysiology |
| doi_str_mv |
10.1152/jn.2001.85.4.1772 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1Mi9qbi4yMDAxLjg1LjQuMTc3Mg |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1Mi9qbi4yMDAxLjg1LjQuMTc3Mg |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 |
| imprint |
American Physiological Society, 2001 |
| imprint_str_mv |
American Physiological Society, 2001 |
| issn |
1522-1598 0022-3077 |
| issn_str_mv |
1522-1598 0022-3077 |
| language |
English |
| mega_collection |
American Physiological Society (CrossRef) |
| match_str |
johnson2001isolationofthekernelforrespiratoryrhythmgenerationinanovelpreparationtheprebotzingercomplexisland |
| publishDateSort |
2001 |
| publisher |
American Physiological Society |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Neurophysiology |
| source_id |
49 |
| title |
Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” |
| title_unstemmed |
Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” |
| title_full |
Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” |
| title_fullStr |
Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” |
| title_full_unstemmed |
Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” |
| title_short |
Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” |
| title_sort |
isolation of the kernel for respiratory rhythm generation in a novel preparation: the pre-bötzinger complex “island” |
| topic |
Physiology General Neuroscience |
| url |
http://dx.doi.org/10.1152/jn.2001.85.4.1772 |
| publishDate |
2001 |
| physical |
1772-1776 |
| description |
<jats:p> The pre-Bötzinger complex (pre-BötC), a bilaterally distributed network of rhythmogenic neurons within the ventrolateral medulla, has been proposed to be the critical locus for respiratory rhythm generation in mammals. To date, thin transverse medullary slice preparations that capture the pre-BötC have served as the optimal experimental model to study the region's inherent cellular and network properties. We have reduced the thin slices to isolated pre-BötC “islands” to further establish whether the pre-BötC has intrinsic rhythmicity and is the kernel for rhythmogenesis in the slice. We recorded neuron population activity locally in the pre-BötC with macroelectrodes and fluorescent imaging of Ca<jats:sup>2+</jats:sup> activities with Calcium Green-1AM dye before and after excising the island. The isolated island remained rhythmically active with a population burst profile similar to the inspiratory burst in the slice. Rhythmic population activity persisted in islands after block of GABA<jats:sub>A</jats:sub>ergic and glycinergic synaptic inhibition. The loci of pre-BötC Ca<jats:sup>2+</jats:sup> activity imaged in thin slices and islands were similar, and imaged pre-BötC neurons exhibited synchronized flashing after blocking synaptic inhibition. Population burst frequency increased monotonically as extracellular potassium concentration was elevated, consistent with mathematical models consisting entirely of an excitatory network of synaptically coupled pacemaker neurons with heterogeneous, voltage-dependent bursting properties. Our results provide further evidence for a rhythmogenic kernel in the pre-BötC in vitro and demonstrate that the islands are ideal preparations for studying the kernel's intrinsic properties. </jats:p> |
| container_issue |
4 |
| container_start_page |
1772 |
| container_title |
Journal of Neurophysiology |
| container_volume |
85 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792341884330311688 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T16:27:01.258Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Isolation+of+the+Kernel+for+Respiratory+Rhythm+Generation+in+a+Novel+Preparation%3A+The+Pre-B%C3%B6tzinger+Complex+%E2%80%9CIsland%E2%80%9D&rft.date=2001-04-01&genre=article&issn=1522-1598&volume=85&issue=4&spage=1772&epage=1776&pages=1772-1776&jtitle=Journal+of+Neurophysiology&atitle=Isolation+of+the+Kernel+for+Respiratory+Rhythm+Generation+in+a+Novel+Preparation%3A+The+Pre-B%C3%B6tzinger+Complex+%E2%80%9CIsland%E2%80%9D&aulast=Smith&aufirst=Jeffrey+C.&rft_id=info%3Adoi%2F10.1152%2Fjn.2001.85.4.1772&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792341884330311688 |
| author | Johnson, Shereé M., Koshiya, Naohiro, Smith, Jeffrey C. |
| author_facet | Johnson, Shereé M., Koshiya, Naohiro, Smith, Jeffrey C., Johnson, Shereé M., Koshiya, Naohiro, Smith, Jeffrey C. |
| author_sort | johnson, shereé m. |
| container_issue | 4 |
| container_start_page | 1772 |
| container_title | Journal of Neurophysiology |
| container_volume | 85 |
| description | <jats:p> The pre-Bötzinger complex (pre-BötC), a bilaterally distributed network of rhythmogenic neurons within the ventrolateral medulla, has been proposed to be the critical locus for respiratory rhythm generation in mammals. To date, thin transverse medullary slice preparations that capture the pre-BötC have served as the optimal experimental model to study the region's inherent cellular and network properties. We have reduced the thin slices to isolated pre-BötC “islands” to further establish whether the pre-BötC has intrinsic rhythmicity and is the kernel for rhythmogenesis in the slice. We recorded neuron population activity locally in the pre-BötC with macroelectrodes and fluorescent imaging of Ca<jats:sup>2+</jats:sup> activities with Calcium Green-1AM dye before and after excising the island. The isolated island remained rhythmically active with a population burst profile similar to the inspiratory burst in the slice. Rhythmic population activity persisted in islands after block of GABA<jats:sub>A</jats:sub>ergic and glycinergic synaptic inhibition. The loci of pre-BötC Ca<jats:sup>2+</jats:sup> activity imaged in thin slices and islands were similar, and imaged pre-BötC neurons exhibited synchronized flashing after blocking synaptic inhibition. Population burst frequency increased monotonically as extracellular potassium concentration was elevated, consistent with mathematical models consisting entirely of an excitatory network of synaptically coupled pacemaker neurons with heterogeneous, voltage-dependent bursting properties. Our results provide further evidence for a rhythmogenic kernel in the pre-BötC in vitro and demonstrate that the islands are ideal preparations for studying the kernel's intrinsic properties. </jats:p> |
| doi_str_mv | 10.1152/jn.2001.85.4.1772 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1Mi9qbi4yMDAxLjg1LjQuMTc3Mg |
| imprint | American Physiological Society, 2001 |
| imprint_str_mv | American Physiological Society, 2001 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 1522-1598, 0022-3077 |
| issn_str_mv | 1522-1598, 0022-3077 |
| language | English |
| last_indexed | 2024-03-01T16:27:01.258Z |
| match_str | johnson2001isolationofthekernelforrespiratoryrhythmgenerationinanovelpreparationtheprebotzingercomplexisland |
| mega_collection | American Physiological Society (CrossRef) |
| physical | 1772-1776 |
| publishDate | 2001 |
| publishDateSort | 2001 |
| publisher | American Physiological Society |
| record_format | ai |
| recordtype | ai |
| series | Journal of Neurophysiology |
| source_id | 49 |
| spelling | Johnson, Shereé M. Koshiya, Naohiro Smith, Jeffrey C. 0022-3077 1522-1598 American Physiological Society Physiology General Neuroscience http://dx.doi.org/10.1152/jn.2001.85.4.1772 <jats:p> The pre-Bötzinger complex (pre-BötC), a bilaterally distributed network of rhythmogenic neurons within the ventrolateral medulla, has been proposed to be the critical locus for respiratory rhythm generation in mammals. To date, thin transverse medullary slice preparations that capture the pre-BötC have served as the optimal experimental model to study the region's inherent cellular and network properties. We have reduced the thin slices to isolated pre-BötC “islands” to further establish whether the pre-BötC has intrinsic rhythmicity and is the kernel for rhythmogenesis in the slice. We recorded neuron population activity locally in the pre-BötC with macroelectrodes and fluorescent imaging of Ca<jats:sup>2+</jats:sup> activities with Calcium Green-1AM dye before and after excising the island. The isolated island remained rhythmically active with a population burst profile similar to the inspiratory burst in the slice. Rhythmic population activity persisted in islands after block of GABA<jats:sub>A</jats:sub>ergic and glycinergic synaptic inhibition. The loci of pre-BötC Ca<jats:sup>2+</jats:sup> activity imaged in thin slices and islands were similar, and imaged pre-BötC neurons exhibited synchronized flashing after blocking synaptic inhibition. Population burst frequency increased monotonically as extracellular potassium concentration was elevated, consistent with mathematical models consisting entirely of an excitatory network of synaptically coupled pacemaker neurons with heterogeneous, voltage-dependent bursting properties. Our results provide further evidence for a rhythmogenic kernel in the pre-BötC in vitro and demonstrate that the islands are ideal preparations for studying the kernel's intrinsic properties. </jats:p> Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” Journal of Neurophysiology |
| spellingShingle | Johnson, Shereé M., Koshiya, Naohiro, Smith, Jeffrey C., Journal of Neurophysiology, Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island”, Physiology, General Neuroscience |
| title | Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” |
| title_full | Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” |
| title_fullStr | Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” |
| title_full_unstemmed | Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” |
| title_short | Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” |
| title_sort | isolation of the kernel for respiratory rhythm generation in a novel preparation: the pre-bötzinger complex “island” |
| title_unstemmed | Isolation of the Kernel for Respiratory Rhythm Generation in a Novel Preparation: The Pre-Bötzinger Complex “Island” |
| topic | Physiology, General Neuroscience |
| url | http://dx.doi.org/10.1152/jn.2001.85.4.1772 |