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Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas
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| Zeitschriftentitel: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 274, 1998, 2, S. R486-R493 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
|
| Schlagwörter: |
| author_facet |
Vilella, Sebastiano Zonno, Vincenzo Ingrosso, Laura Verri, Tiziano Storelli, Carlo Vilella, Sebastiano Zonno, Vincenzo Ingrosso, Laura Verri, Tiziano Storelli, Carlo |
|---|---|
| author |
Vilella, Sebastiano Zonno, Vincenzo Ingrosso, Laura Verri, Tiziano Storelli, Carlo |
| spellingShingle |
Vilella, Sebastiano Zonno, Vincenzo Ingrosso, Laura Verri, Tiziano Storelli, Carlo American Journal of Physiology-Regulatory, Integrative and Comparative Physiology Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas Physiology (medical) Physiology |
| author_sort |
vilella, sebastiano |
| spelling |
Vilella, Sebastiano Zonno, Vincenzo Ingrosso, Laura Verri, Tiziano Storelli, Carlo 0363-6119 1522-1490 American Physiological Society Physiology (medical) Physiology http://dx.doi.org/10.1152/ajpregu.1998.274.2.r486 <jats:p>An electroneutral Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>exchange mechanism (dimethylamiloride inhibitable, Li<jats:sup>+</jats:sup>sensitive, and Ca<jats:sup>2+</jats:sup>insensitive) was identified in brush-border membrane vesicles (BBMV) from Kuruma prawn hepatopancreas by monitoring Na<jats:sup>+</jats:sup>-dependent H<jats:sup>+</jats:sup>fluxes with the pH-sensitive dye acridine orange and measuring<jats:sup>22</jats:sup>Na<jats:sup>+</jats:sup>uptake. Kinetic parameters measured under short-circuited conditions were the Na<jats:sup>+</jats:sup>concentration that yielded one-half of the maximal dissipation rate ( F<jats:sub>max</jats:sub>) of the preset transmembrane ΔpH ( K<jats:sub>Na</jats:sub>) = 15 ± 2 mM and F<jats:sub>max</jats:sub>= 3,626 ± 197 Δ F ⋅ min<jats:sup>−1</jats:sup>⋅ mg protein<jats:sup>−1</jats:sup>, with a Hill coefficient for Na<jats:sup>+</jats:sup>of ∼1. In addition, the inhibitory constant for dimethylamiloride was found to be ∼1 μM. The electroneutral nature of the antiporter was assessed in that an inside-negative transmembrane electrical potential neither affected kinetic parameters nor stimulated pH-dependent (intracellular pH > extracellular pH)<jats:sup>22</jats:sup>Na<jats:sup>+</jats:sup>uptake. In contrast, electrogenic pH-dependent<jats:sup>22</jats:sup>Na<jats:sup>+</jats:sup>uptake was observed in lobster hepatopancreatic BBMV. Substitution of chloride with gluconate resulted in increasing K<jats:sub>Na</jats:sub>and decreasing Δ F<jats:sub>max</jats:sub>, which suggests a possible role of chloride in the operational mechanism of the antiporter. These results indicate that a Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>exchanger, resembling the electroneutral Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>antiporter model, is present in hepatopancreatic BBMV from the Kuruma prawn Penaeus japonicus.</jats:p> Electroneutral Na<sup>+</sup>/H<sup>+</sup>exchange in brush-border membrane vesicles from<i>Penaeus japonicus</i>hepatopancreas American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
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10.1152/ajpregu.1998.274.2.r486 |
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Online Free |
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American Physiological Society, 1998 |
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American Physiological Society, 1998 |
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1522-1490 0363-6119 |
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1998 |
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American Physiological Society |
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American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
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| title |
Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas |
| title_unstemmed |
Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas |
| title_full |
Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas |
| title_fullStr |
Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas |
| title_full_unstemmed |
Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas |
| title_short |
Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas |
| title_sort |
electroneutral na<sup>+</sup>/h<sup>+</sup>exchange in brush-border membrane vesicles from<i>penaeus japonicus</i>hepatopancreas |
| topic |
Physiology (medical) Physiology |
| url |
http://dx.doi.org/10.1152/ajpregu.1998.274.2.r486 |
| publishDate |
1998 |
| physical |
R486-R493 |
| description |
<jats:p>An electroneutral Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>exchange mechanism (dimethylamiloride inhibitable, Li<jats:sup>+</jats:sup>sensitive, and Ca<jats:sup>2+</jats:sup>insensitive) was identified in brush-border membrane vesicles (BBMV) from Kuruma prawn hepatopancreas by monitoring Na<jats:sup>+</jats:sup>-dependent H<jats:sup>+</jats:sup>fluxes with the pH-sensitive dye acridine orange and measuring<jats:sup>22</jats:sup>Na<jats:sup>+</jats:sup>uptake. Kinetic parameters measured under short-circuited conditions were the Na<jats:sup>+</jats:sup>concentration that yielded one-half of the maximal dissipation rate ( F<jats:sub>max</jats:sub>) of the preset transmembrane ΔpH ( K<jats:sub>Na</jats:sub>) = 15 ± 2 mM and F<jats:sub>max</jats:sub>= 3,626 ± 197 Δ F ⋅ min<jats:sup>−1</jats:sup>⋅ mg protein<jats:sup>−1</jats:sup>, with a Hill coefficient for Na<jats:sup>+</jats:sup>of ∼1. In addition, the inhibitory constant for dimethylamiloride was found to be ∼1 μM. The electroneutral nature of the antiporter was assessed in that an inside-negative transmembrane electrical potential neither affected kinetic parameters nor stimulated pH-dependent (intracellular pH > extracellular pH)<jats:sup>22</jats:sup>Na<jats:sup>+</jats:sup>uptake. In contrast, electrogenic pH-dependent<jats:sup>22</jats:sup>Na<jats:sup>+</jats:sup>uptake was observed in lobster hepatopancreatic BBMV. Substitution of chloride with gluconate resulted in increasing K<jats:sub>Na</jats:sub>and decreasing Δ F<jats:sub>max</jats:sub>, which suggests a possible role of chloride in the operational mechanism of the antiporter. These results indicate that a Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>exchanger, resembling the electroneutral Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>antiporter model, is present in hepatopancreatic BBMV from the Kuruma prawn Penaeus japonicus.</jats:p> |
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| author | Vilella, Sebastiano, Zonno, Vincenzo, Ingrosso, Laura, Verri, Tiziano, Storelli, Carlo |
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| description | <jats:p>An electroneutral Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>exchange mechanism (dimethylamiloride inhibitable, Li<jats:sup>+</jats:sup>sensitive, and Ca<jats:sup>2+</jats:sup>insensitive) was identified in brush-border membrane vesicles (BBMV) from Kuruma prawn hepatopancreas by monitoring Na<jats:sup>+</jats:sup>-dependent H<jats:sup>+</jats:sup>fluxes with the pH-sensitive dye acridine orange and measuring<jats:sup>22</jats:sup>Na<jats:sup>+</jats:sup>uptake. Kinetic parameters measured under short-circuited conditions were the Na<jats:sup>+</jats:sup>concentration that yielded one-half of the maximal dissipation rate ( F<jats:sub>max</jats:sub>) of the preset transmembrane ΔpH ( K<jats:sub>Na</jats:sub>) = 15 ± 2 mM and F<jats:sub>max</jats:sub>= 3,626 ± 197 Δ F ⋅ min<jats:sup>−1</jats:sup>⋅ mg protein<jats:sup>−1</jats:sup>, with a Hill coefficient for Na<jats:sup>+</jats:sup>of ∼1. In addition, the inhibitory constant for dimethylamiloride was found to be ∼1 μM. The electroneutral nature of the antiporter was assessed in that an inside-negative transmembrane electrical potential neither affected kinetic parameters nor stimulated pH-dependent (intracellular pH > extracellular pH)<jats:sup>22</jats:sup>Na<jats:sup>+</jats:sup>uptake. In contrast, electrogenic pH-dependent<jats:sup>22</jats:sup>Na<jats:sup>+</jats:sup>uptake was observed in lobster hepatopancreatic BBMV. Substitution of chloride with gluconate resulted in increasing K<jats:sub>Na</jats:sub>and decreasing Δ F<jats:sub>max</jats:sub>, which suggests a possible role of chloride in the operational mechanism of the antiporter. These results indicate that a Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>exchanger, resembling the electroneutral Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>antiporter model, is present in hepatopancreatic BBMV from the Kuruma prawn Penaeus japonicus.</jats:p> |
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| imprint | American Physiological Society, 1998 |
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| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2 |
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| physical | R486-R493 |
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| spelling | Vilella, Sebastiano Zonno, Vincenzo Ingrosso, Laura Verri, Tiziano Storelli, Carlo 0363-6119 1522-1490 American Physiological Society Physiology (medical) Physiology http://dx.doi.org/10.1152/ajpregu.1998.274.2.r486 <jats:p>An electroneutral Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>exchange mechanism (dimethylamiloride inhibitable, Li<jats:sup>+</jats:sup>sensitive, and Ca<jats:sup>2+</jats:sup>insensitive) was identified in brush-border membrane vesicles (BBMV) from Kuruma prawn hepatopancreas by monitoring Na<jats:sup>+</jats:sup>-dependent H<jats:sup>+</jats:sup>fluxes with the pH-sensitive dye acridine orange and measuring<jats:sup>22</jats:sup>Na<jats:sup>+</jats:sup>uptake. Kinetic parameters measured under short-circuited conditions were the Na<jats:sup>+</jats:sup>concentration that yielded one-half of the maximal dissipation rate ( F<jats:sub>max</jats:sub>) of the preset transmembrane ΔpH ( K<jats:sub>Na</jats:sub>) = 15 ± 2 mM and F<jats:sub>max</jats:sub>= 3,626 ± 197 Δ F ⋅ min<jats:sup>−1</jats:sup>⋅ mg protein<jats:sup>−1</jats:sup>, with a Hill coefficient for Na<jats:sup>+</jats:sup>of ∼1. In addition, the inhibitory constant for dimethylamiloride was found to be ∼1 μM. The electroneutral nature of the antiporter was assessed in that an inside-negative transmembrane electrical potential neither affected kinetic parameters nor stimulated pH-dependent (intracellular pH > extracellular pH)<jats:sup>22</jats:sup>Na<jats:sup>+</jats:sup>uptake. In contrast, electrogenic pH-dependent<jats:sup>22</jats:sup>Na<jats:sup>+</jats:sup>uptake was observed in lobster hepatopancreatic BBMV. Substitution of chloride with gluconate resulted in increasing K<jats:sub>Na</jats:sub>and decreasing Δ F<jats:sub>max</jats:sub>, which suggests a possible role of chloride in the operational mechanism of the antiporter. These results indicate that a Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>exchanger, resembling the electroneutral Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>antiporter model, is present in hepatopancreatic BBMV from the Kuruma prawn Penaeus japonicus.</jats:p> Electroneutral Na<sup>+</sup>/H<sup>+</sup>exchange in brush-border membrane vesicles from<i>Penaeus japonicus</i>hepatopancreas American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
| spellingShingle | Vilella, Sebastiano, Zonno, Vincenzo, Ingrosso, Laura, Verri, Tiziano, Storelli, Carlo, American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas, Physiology (medical), Physiology |
| title | Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas |
| title_full | Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas |
| title_fullStr | Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas |
| title_full_unstemmed | Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas |
| title_short | Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas |
| title_sort | electroneutral na<sup>+</sup>/h<sup>+</sup>exchange in brush-border membrane vesicles from<i>penaeus japonicus</i>hepatopancreas |
| title_unstemmed | Electroneutral Na+/H+exchange in brush-border membrane vesicles fromPenaeus japonicushepatopancreas |
| topic | Physiology (medical), Physiology |
| url | http://dx.doi.org/10.1152/ajpregu.1998.274.2.r486 |