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Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride
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| Zeitschriftentitel: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
|---|---|
| Personen und Körperschaften: | , |
| In: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 284, 2003, 2, S. R380-R388 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
|
| Schlagwörter: |
| author_facet |
Pelis, Ryan M. Renfro, J. Larry Pelis, Ryan M. Renfro, J. Larry |
|---|---|
| author |
Pelis, Ryan M. Renfro, J. Larry |
| spellingShingle |
Pelis, Ryan M. Renfro, J. Larry American Journal of Physiology-Regulatory, Integrative and Comparative Physiology Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride Physiology (medical) Physiology |
| author_sort |
pelis, ryan m. |
| spelling |
Pelis, Ryan M. Renfro, J. Larry 0363-6119 1522-1490 American Physiological Society Physiology (medical) Physiology http://dx.doi.org/10.1152/ajpregu.00573.2002 <jats:p>SO[Formula: see text]transport by winter flounder intestine in Ussing chambers was characterized. With 50 mM SO[Formula: see text] (physiological level) bathing the lumen, net absorption (lumen to blood) dominated. Under short-circuited conditions, 1 mM SO[Formula: see text] on both sides, net active SO[Formula: see text] secretion occurred (8.55 ± 0.96 nmol · cm<jats:sup>−2</jats:sup>· h<jats:sup>−1</jats:sup>). NaCN (10 mM), ouabain (10<jats:sup>−4</jats:sup>M), and luminal DIDS (0.2 mM) inhibited net secretion. Removal of luminal Cl<jats:sup>−</jats:sup>and HCO[Formula: see text] together (Cl<jats:sup>−</jats:sup>-HCO[Formula: see text]) or Cl<jats:sup>−</jats:sup>alone blocked net secretion, whereas removal of luminal HCO[Formula: see text] alone increased net secretion. SO[Formula: see text] uptake into foregut brush-border membrane vesicles was stimulated by a trans-Cl<jats:sup>−</jats:sup>gradient (in > out) and unaffected by a trans-HCO[Formula: see text] gradient (in > out). Short-circuiting with K<jats:sup>+</jats:sup>(in = out) and valinomycin had no effect on Cl<jats:sup>−</jats:sup>-stimulated SO[Formula: see text] uptake, suggesting electroneutral exchange. Satiety (i.e., full stomach) stimulated the unidirectional absorptive flux, eliminating net secretion. It was concluded that the intestine is a site of SO[Formula: see text] absorption in marine teleosts and that active SO[Formula: see text] secretion is in exchange for luminal Cl<jats:sup>−</jats:sup>.</jats:p> Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
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10.1152/ajpregu.00573.2002 |
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American Physiological Society, 2003 |
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| title |
Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| title_unstemmed |
Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| title_full |
Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| title_fullStr |
Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| title_full_unstemmed |
Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| title_short |
Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| title_sort |
active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| topic |
Physiology (medical) Physiology |
| url |
http://dx.doi.org/10.1152/ajpregu.00573.2002 |
| publishDate |
2003 |
| physical |
R380-R388 |
| description |
<jats:p>SO[Formula: see text]transport by winter flounder intestine in Ussing chambers was characterized. With 50 mM SO[Formula: see text] (physiological level) bathing the lumen, net absorption (lumen to blood) dominated. Under short-circuited conditions, 1 mM SO[Formula: see text] on both sides, net active SO[Formula: see text] secretion occurred (8.55 ± 0.96 nmol · cm<jats:sup>−2</jats:sup>· h<jats:sup>−1</jats:sup>). NaCN (10 mM), ouabain (10<jats:sup>−4</jats:sup>M), and luminal DIDS (0.2 mM) inhibited net secretion. Removal of luminal Cl<jats:sup>−</jats:sup>and HCO[Formula: see text] together (Cl<jats:sup>−</jats:sup>-HCO[Formula: see text]) or Cl<jats:sup>−</jats:sup>alone blocked net secretion, whereas removal of luminal HCO[Formula: see text] alone increased net secretion. SO[Formula: see text] uptake into foregut brush-border membrane vesicles was stimulated by a trans-Cl<jats:sup>−</jats:sup>gradient (in > out) and unaffected by a trans-HCO[Formula: see text] gradient (in > out). Short-circuiting with K<jats:sup>+</jats:sup>(in = out) and valinomycin had no effect on Cl<jats:sup>−</jats:sup>-stimulated SO[Formula: see text] uptake, suggesting electroneutral exchange. Satiety (i.e., full stomach) stimulated the unidirectional absorptive flux, eliminating net secretion. It was concluded that the intestine is a site of SO[Formula: see text] absorption in marine teleosts and that active SO[Formula: see text] secretion is in exchange for luminal Cl<jats:sup>−</jats:sup>.</jats:p> |
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| description | <jats:p>SO[Formula: see text]transport by winter flounder intestine in Ussing chambers was characterized. With 50 mM SO[Formula: see text] (physiological level) bathing the lumen, net absorption (lumen to blood) dominated. Under short-circuited conditions, 1 mM SO[Formula: see text] on both sides, net active SO[Formula: see text] secretion occurred (8.55 ± 0.96 nmol · cm<jats:sup>−2</jats:sup>· h<jats:sup>−1</jats:sup>). NaCN (10 mM), ouabain (10<jats:sup>−4</jats:sup>M), and luminal DIDS (0.2 mM) inhibited net secretion. Removal of luminal Cl<jats:sup>−</jats:sup>and HCO[Formula: see text] together (Cl<jats:sup>−</jats:sup>-HCO[Formula: see text]) or Cl<jats:sup>−</jats:sup>alone blocked net secretion, whereas removal of luminal HCO[Formula: see text] alone increased net secretion. SO[Formula: see text] uptake into foregut brush-border membrane vesicles was stimulated by a trans-Cl<jats:sup>−</jats:sup>gradient (in > out) and unaffected by a trans-HCO[Formula: see text] gradient (in > out). Short-circuiting with K<jats:sup>+</jats:sup>(in = out) and valinomycin had no effect on Cl<jats:sup>−</jats:sup>-stimulated SO[Formula: see text] uptake, suggesting electroneutral exchange. Satiety (i.e., full stomach) stimulated the unidirectional absorptive flux, eliminating net secretion. It was concluded that the intestine is a site of SO[Formula: see text] absorption in marine teleosts and that active SO[Formula: see text] secretion is in exchange for luminal Cl<jats:sup>−</jats:sup>.</jats:p> |
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| spelling | Pelis, Ryan M. Renfro, J. Larry 0363-6119 1522-1490 American Physiological Society Physiology (medical) Physiology http://dx.doi.org/10.1152/ajpregu.00573.2002 <jats:p>SO[Formula: see text]transport by winter flounder intestine in Ussing chambers was characterized. With 50 mM SO[Formula: see text] (physiological level) bathing the lumen, net absorption (lumen to blood) dominated. Under short-circuited conditions, 1 mM SO[Formula: see text] on both sides, net active SO[Formula: see text] secretion occurred (8.55 ± 0.96 nmol · cm<jats:sup>−2</jats:sup>· h<jats:sup>−1</jats:sup>). NaCN (10 mM), ouabain (10<jats:sup>−4</jats:sup>M), and luminal DIDS (0.2 mM) inhibited net secretion. Removal of luminal Cl<jats:sup>−</jats:sup>and HCO[Formula: see text] together (Cl<jats:sup>−</jats:sup>-HCO[Formula: see text]) or Cl<jats:sup>−</jats:sup>alone blocked net secretion, whereas removal of luminal HCO[Formula: see text] alone increased net secretion. SO[Formula: see text] uptake into foregut brush-border membrane vesicles was stimulated by a trans-Cl<jats:sup>−</jats:sup>gradient (in > out) and unaffected by a trans-HCO[Formula: see text] gradient (in > out). Short-circuiting with K<jats:sup>+</jats:sup>(in = out) and valinomycin had no effect on Cl<jats:sup>−</jats:sup>-stimulated SO[Formula: see text] uptake, suggesting electroneutral exchange. Satiety (i.e., full stomach) stimulated the unidirectional absorptive flux, eliminating net secretion. It was concluded that the intestine is a site of SO[Formula: see text] absorption in marine teleosts and that active SO[Formula: see text] secretion is in exchange for luminal Cl<jats:sup>−</jats:sup>.</jats:p> Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
| spellingShingle | Pelis, Ryan M., Renfro, J. Larry, American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride, Physiology (medical), Physiology |
| title | Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| title_full | Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| title_fullStr | Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| title_full_unstemmed | Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| title_short | Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| title_sort | active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| title_unstemmed | Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride |
| topic | Physiology (medical), Physiology |
| url | http://dx.doi.org/10.1152/ajpregu.00573.2002 |