Eintrag weiter verarbeiten
Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes
Gespeichert in:
| Zeitschriftentitel: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 296, 2009, 6, S. R1897-R1906 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
|
| Schlagwörter: |
| author_facet |
Liao, Bo-Kai Chen, Ruo-Dong Hwang, Pung-Pung Liao, Bo-Kai Chen, Ruo-Dong Hwang, Pung-Pung |
|---|---|
| author |
Liao, Bo-Kai Chen, Ruo-Dong Hwang, Pung-Pung |
| spellingShingle |
Liao, Bo-Kai Chen, Ruo-Dong Hwang, Pung-Pung American Journal of Physiology-Regulatory, Integrative and Comparative Physiology Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes Physiology (medical) Physiology |
| author_sort |
liao, bo-kai |
| spelling |
Liao, Bo-Kai Chen, Ruo-Dong Hwang, Pung-Pung 0363-6119 1522-1490 American Physiological Society Physiology (medical) Physiology http://dx.doi.org/10.1152/ajpregu.00029.2009 <jats:p>In zebrafish ( Danio rerio), six distinct Na<jats:sup>+</jats:sup>-K<jats:sup>+</jats:sup>-ATPase (NKA) α1-subunit genes have been identified, and four of them, zatp1a1a.1, zatp1a1a.2, zatp1a1a.4, and zatp1a1a.5, are expressed in embryonic skin where different types of ionocytes appear. The present study attempted to test a hypothesis of whether these NKA α1 paralogues are specifically expressed and function in respective ionocytes. Double fluorescence in situ hybridization analysis demonstrated the specific expression of zatp1a1a.1, zatp1a1a.2, and zatp1a1a. 5 in NKA-rich (NaR) cells, Na<jats:sup>+</jats:sup>-Cl<jats:sup>−</jats:sup>cotransporter (NCC)-expressing cells, and H<jats:sup>+</jats:sup>-ATPase-rich (HR) cells, respectively, based on the colocalization of the three NKA α1 genes with marker genes of the respective ionocytes (epithelial Ca<jats:sup>2+</jats:sup>channel in NaR cells; NCC in NCC cells; and H<jats:sup>+</jats:sup>-ATPase and Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>exchanger 3b in HR cells). The mRNA expression (by real-time PCR) of zatp1a1a.1, zatp1a1a.2, and zatp1a1a.5 were, respectively, upregulated by low-Ca<jats:sup>2+</jats:sup>, low-Cl<jats:sup>−</jats:sup>, and low-Na<jats:sup>+</jats:sup>freshwater, which had previously been reported to stimulate uptake functions of Ca<jats:sup>2+</jats:sup>, Cl<jats:sup>−</jats:sup>, and Na<jats:sup>+</jats:sup>. However, zatp1a1a.4 was not colocalized with any of the three types of ionocytes, nor did its mRNA respond to the ambient ions examined. Taken together, zATP1a1a.1, zATP1a1a.2, and zATP1a1a.5 may provide driving force for Na<jats:sup>+</jats:sup>-coupled cotransporter activity specifically in NaR, NCC, and HR cells, respectively.</jats:p> Expression regulation of Na<sup>+</sup>-K<sup>+</sup>-ATPase α1-subunit subtypes in zebrafish gill ionocytes American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
| doi_str_mv |
10.1152/ajpregu.00029.2009 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1Mi9hanByZWd1LjAwMDI5LjIwMDk |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1Mi9hanByZWd1LjAwMDI5LjIwMDk |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Zi4 DE-Gla1 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 |
| imprint |
American Physiological Society, 2009 |
| imprint_str_mv |
American Physiological Society, 2009 |
| issn |
0363-6119 1522-1490 |
| issn_str_mv |
0363-6119 1522-1490 |
| language |
English |
| mega_collection |
American Physiological Society (CrossRef) |
| match_str |
liao2009expressionregulationofnakatpasea1subunitsubtypesinzebrafishgillionocytes |
| publishDateSort |
2009 |
| publisher |
American Physiological Society |
| recordtype |
ai |
| record_format |
ai |
| series |
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
| source_id |
49 |
| title |
Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes |
| title_unstemmed |
Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes |
| title_full |
Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes |
| title_fullStr |
Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes |
| title_full_unstemmed |
Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes |
| title_short |
Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes |
| title_sort |
expression regulation of na<sup>+</sup>-k<sup>+</sup>-atpase α1-subunit subtypes in zebrafish gill ionocytes |
| topic |
Physiology (medical) Physiology |
| url |
http://dx.doi.org/10.1152/ajpregu.00029.2009 |
| publishDate |
2009 |
| physical |
R1897-R1906 |
| description |
<jats:p>In zebrafish ( Danio rerio), six distinct Na<jats:sup>+</jats:sup>-K<jats:sup>+</jats:sup>-ATPase (NKA) α1-subunit genes have been identified, and four of them, zatp1a1a.1, zatp1a1a.2, zatp1a1a.4, and zatp1a1a.5, are expressed in embryonic skin where different types of ionocytes appear. The present study attempted to test a hypothesis of whether these NKA α1 paralogues are specifically expressed and function in respective ionocytes. Double fluorescence in situ hybridization analysis demonstrated the specific expression of zatp1a1a.1, zatp1a1a.2, and zatp1a1a. 5 in NKA-rich (NaR) cells, Na<jats:sup>+</jats:sup>-Cl<jats:sup>−</jats:sup>cotransporter (NCC)-expressing cells, and H<jats:sup>+</jats:sup>-ATPase-rich (HR) cells, respectively, based on the colocalization of the three NKA α1 genes with marker genes of the respective ionocytes (epithelial Ca<jats:sup>2+</jats:sup>channel in NaR cells; NCC in NCC cells; and H<jats:sup>+</jats:sup>-ATPase and Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>exchanger 3b in HR cells). The mRNA expression (by real-time PCR) of zatp1a1a.1, zatp1a1a.2, and zatp1a1a.5 were, respectively, upregulated by low-Ca<jats:sup>2+</jats:sup>, low-Cl<jats:sup>−</jats:sup>, and low-Na<jats:sup>+</jats:sup>freshwater, which had previously been reported to stimulate uptake functions of Ca<jats:sup>2+</jats:sup>, Cl<jats:sup>−</jats:sup>, and Na<jats:sup>+</jats:sup>. However, zatp1a1a.4 was not colocalized with any of the three types of ionocytes, nor did its mRNA respond to the ambient ions examined. Taken together, zATP1a1a.1, zATP1a1a.2, and zATP1a1a.5 may provide driving force for Na<jats:sup>+</jats:sup>-coupled cotransporter activity specifically in NaR, NCC, and HR cells, respectively.</jats:p> |
| container_issue |
6 |
| container_start_page |
0 |
| container_title |
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
| container_volume |
296 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792342432181911559 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T16:35:42.665Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Expression+regulation+of+Na%2B-K%2B-ATPase+%CE%B11-subunit+subtypes+in+zebrafish+gill+ionocytes&rft.date=2009-06-01&genre=article&issn=1522-1490&volume=296&issue=6&pages=R1897-R1906&jtitle=American+Journal+of+Physiology-Regulatory%2C+Integrative+and+Comparative+Physiology&atitle=Expression+regulation+of+Na%3Csup%3E%2B%3C%2Fsup%3E-K%3Csup%3E%2B%3C%2Fsup%3E-ATPase+%CE%B11-subunit+subtypes+in+zebrafish+gill+ionocytes&aulast=Hwang&aufirst=Pung-Pung&rft_id=info%3Adoi%2F10.1152%2Fajpregu.00029.2009&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792342432181911559 |
| author | Liao, Bo-Kai, Chen, Ruo-Dong, Hwang, Pung-Pung |
| author_facet | Liao, Bo-Kai, Chen, Ruo-Dong, Hwang, Pung-Pung, Liao, Bo-Kai, Chen, Ruo-Dong, Hwang, Pung-Pung |
| author_sort | liao, bo-kai |
| container_issue | 6 |
| container_start_page | 0 |
| container_title | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
| container_volume | 296 |
| description | <jats:p>In zebrafish ( Danio rerio), six distinct Na<jats:sup>+</jats:sup>-K<jats:sup>+</jats:sup>-ATPase (NKA) α1-subunit genes have been identified, and four of them, zatp1a1a.1, zatp1a1a.2, zatp1a1a.4, and zatp1a1a.5, are expressed in embryonic skin where different types of ionocytes appear. The present study attempted to test a hypothesis of whether these NKA α1 paralogues are specifically expressed and function in respective ionocytes. Double fluorescence in situ hybridization analysis demonstrated the specific expression of zatp1a1a.1, zatp1a1a.2, and zatp1a1a. 5 in NKA-rich (NaR) cells, Na<jats:sup>+</jats:sup>-Cl<jats:sup>−</jats:sup>cotransporter (NCC)-expressing cells, and H<jats:sup>+</jats:sup>-ATPase-rich (HR) cells, respectively, based on the colocalization of the three NKA α1 genes with marker genes of the respective ionocytes (epithelial Ca<jats:sup>2+</jats:sup>channel in NaR cells; NCC in NCC cells; and H<jats:sup>+</jats:sup>-ATPase and Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>exchanger 3b in HR cells). The mRNA expression (by real-time PCR) of zatp1a1a.1, zatp1a1a.2, and zatp1a1a.5 were, respectively, upregulated by low-Ca<jats:sup>2+</jats:sup>, low-Cl<jats:sup>−</jats:sup>, and low-Na<jats:sup>+</jats:sup>freshwater, which had previously been reported to stimulate uptake functions of Ca<jats:sup>2+</jats:sup>, Cl<jats:sup>−</jats:sup>, and Na<jats:sup>+</jats:sup>. However, zatp1a1a.4 was not colocalized with any of the three types of ionocytes, nor did its mRNA respond to the ambient ions examined. Taken together, zATP1a1a.1, zATP1a1a.2, and zATP1a1a.5 may provide driving force for Na<jats:sup>+</jats:sup>-coupled cotransporter activity specifically in NaR, NCC, and HR cells, respectively.</jats:p> |
| doi_str_mv | 10.1152/ajpregu.00029.2009 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1Mi9hanByZWd1LjAwMDI5LjIwMDk |
| imprint | American Physiological Society, 2009 |
| imprint_str_mv | American Physiological Society, 2009 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229 |
| issn | 0363-6119, 1522-1490 |
| issn_str_mv | 0363-6119, 1522-1490 |
| language | English |
| last_indexed | 2024-03-01T16:35:42.665Z |
| match_str | liao2009expressionregulationofnakatpasea1subunitsubtypesinzebrafishgillionocytes |
| mega_collection | American Physiological Society (CrossRef) |
| physical | R1897-R1906 |
| publishDate | 2009 |
| publishDateSort | 2009 |
| publisher | American Physiological Society |
| record_format | ai |
| recordtype | ai |
| series | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
| source_id | 49 |
| spelling | Liao, Bo-Kai Chen, Ruo-Dong Hwang, Pung-Pung 0363-6119 1522-1490 American Physiological Society Physiology (medical) Physiology http://dx.doi.org/10.1152/ajpregu.00029.2009 <jats:p>In zebrafish ( Danio rerio), six distinct Na<jats:sup>+</jats:sup>-K<jats:sup>+</jats:sup>-ATPase (NKA) α1-subunit genes have been identified, and four of them, zatp1a1a.1, zatp1a1a.2, zatp1a1a.4, and zatp1a1a.5, are expressed in embryonic skin where different types of ionocytes appear. The present study attempted to test a hypothesis of whether these NKA α1 paralogues are specifically expressed and function in respective ionocytes. Double fluorescence in situ hybridization analysis demonstrated the specific expression of zatp1a1a.1, zatp1a1a.2, and zatp1a1a. 5 in NKA-rich (NaR) cells, Na<jats:sup>+</jats:sup>-Cl<jats:sup>−</jats:sup>cotransporter (NCC)-expressing cells, and H<jats:sup>+</jats:sup>-ATPase-rich (HR) cells, respectively, based on the colocalization of the three NKA α1 genes with marker genes of the respective ionocytes (epithelial Ca<jats:sup>2+</jats:sup>channel in NaR cells; NCC in NCC cells; and H<jats:sup>+</jats:sup>-ATPase and Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup>exchanger 3b in HR cells). The mRNA expression (by real-time PCR) of zatp1a1a.1, zatp1a1a.2, and zatp1a1a.5 were, respectively, upregulated by low-Ca<jats:sup>2+</jats:sup>, low-Cl<jats:sup>−</jats:sup>, and low-Na<jats:sup>+</jats:sup>freshwater, which had previously been reported to stimulate uptake functions of Ca<jats:sup>2+</jats:sup>, Cl<jats:sup>−</jats:sup>, and Na<jats:sup>+</jats:sup>. However, zatp1a1a.4 was not colocalized with any of the three types of ionocytes, nor did its mRNA respond to the ambient ions examined. Taken together, zATP1a1a.1, zATP1a1a.2, and zATP1a1a.5 may provide driving force for Na<jats:sup>+</jats:sup>-coupled cotransporter activity specifically in NaR, NCC, and HR cells, respectively.</jats:p> Expression regulation of Na<sup>+</sup>-K<sup>+</sup>-ATPase α1-subunit subtypes in zebrafish gill ionocytes American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
| spellingShingle | Liao, Bo-Kai, Chen, Ruo-Dong, Hwang, Pung-Pung, American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes, Physiology (medical), Physiology |
| title | Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes |
| title_full | Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes |
| title_fullStr | Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes |
| title_full_unstemmed | Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes |
| title_short | Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes |
| title_sort | expression regulation of na<sup>+</sup>-k<sup>+</sup>-atpase α1-subunit subtypes in zebrafish gill ionocytes |
| title_unstemmed | Expression regulation of Na+-K+-ATPase α1-subunit subtypes in zebrafish gill ionocytes |
| topic | Physiology (medical), Physiology |
| url | http://dx.doi.org/10.1152/ajpregu.00029.2009 |