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Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion
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| Zeitschriftentitel: | American Journal of Physiology-Renal Physiology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | American Journal of Physiology-Renal Physiology, 245, 1983, 5, S. F622-F633 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
|
| Schlagwörter: |
| author_facet |
Chan, Y. L. Malnic, G. Giebisch, G. Chan, Y. L. Malnic, G. Giebisch, G. |
|---|---|
| author |
Chan, Y. L. Malnic, G. Giebisch, G. |
| spellingShingle |
Chan, Y. L. Malnic, G. Giebisch, G. American Journal of Physiology-Renal Physiology Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion Physiology |
| author_sort |
chan, y. l. |
| spelling |
Chan, Y. L. Malnic, G. Giebisch, G. 1931-857X 1522-1466 American Physiological Society Physiology http://dx.doi.org/10.1152/ajprenal.1983.245.5.f622 <jats:p> The effect of oncotic pressure changes on fluid (Jv) and net bicarbonate transport (JHCO-3) and the transepithelial bicarbonate permeability (PHCO-3) were measured by an improved luminal and capillary microperfusion method that allows paired experiments on the same tubule. Rat proximal tubules were pump-perfused and Jv and [HCO-3] measured with [14C]inulin and a pH glass electrode. Raising peritubular protein (0-8-15 g/100 ml bovine serum albumin) stimulated Jv and HCO-3 reabsorption. The response to oncotic pressure changes was asymmetrical since changes of the luminal protein concentration had no significant effects. Whereas transepithelial solvent drag effects on HCO-3 must be minimal, peritubular protein most likely stimulates translocation of fluid and bicarbonate from intercellular spaces into peritubular capillaries. PHCO-3 was measured from HCO-3 net flux along a lumen-to-capillary-directed electrochemical potential gradient. In these experiments active H+ transport and Jv were minimized by 10(-4) M acetazolamide and luminal raffinose. PHCO-3 was 1.77 X 10(-5) cm X s-1 and was unaffected by increasing luminal flow rate from 10 to 45 nl X min-1. Since bicarbonate backflux is only a small fraction of physiological rates of JHCO-3, net transport alterations at varying [HCO-3] in the lumen must be due to changes in active HCO-3 (H+) transport. Thus, active H+ ion secretion across the luminal membrane of the proximal tubule is gradient dependent. </jats:p> Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion American Journal of Physiology-Renal Physiology |
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10.1152/ajprenal.1983.245.5.f622 |
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Online |
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Biologie |
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American Physiological Society, 1983 |
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American Physiological Society, 1983 |
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1931-857X 1522-1466 |
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1931-857X 1522-1466 |
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chan1983passivedrivingforcesofproximaltubularfluidandbicarbonatetransportgradientdependenceofhsecretion |
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1983 |
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American Physiological Society |
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ai |
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American Journal of Physiology-Renal Physiology |
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49 |
| title |
Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion |
| title_unstemmed |
Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion |
| title_full |
Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion |
| title_fullStr |
Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion |
| title_full_unstemmed |
Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion |
| title_short |
Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion |
| title_sort |
passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of h+ secretion |
| topic |
Physiology |
| url |
http://dx.doi.org/10.1152/ajprenal.1983.245.5.f622 |
| publishDate |
1983 |
| physical |
F622-F633 |
| description |
<jats:p> The effect of oncotic pressure changes on fluid (Jv) and net bicarbonate transport (JHCO-3) and the transepithelial bicarbonate permeability (PHCO-3) were measured by an improved luminal and capillary microperfusion method that allows paired experiments on the same tubule. Rat proximal tubules were pump-perfused and Jv and [HCO-3] measured with [14C]inulin and a pH glass electrode. Raising peritubular protein (0-8-15 g/100 ml bovine serum albumin) stimulated Jv and HCO-3 reabsorption. The response to oncotic pressure changes was asymmetrical since changes of the luminal protein concentration had no significant effects. Whereas transepithelial solvent drag effects on HCO-3 must be minimal, peritubular protein most likely stimulates translocation of fluid and bicarbonate from intercellular spaces into peritubular capillaries. PHCO-3 was measured from HCO-3 net flux along a lumen-to-capillary-directed electrochemical potential gradient. In these experiments active H+ transport and Jv were minimized by 10(-4) M acetazolamide and luminal raffinose. PHCO-3 was 1.77 X 10(-5) cm X s-1 and was unaffected by increasing luminal flow rate from 10 to 45 nl X min-1. Since bicarbonate backflux is only a small fraction of physiological rates of JHCO-3, net transport alterations at varying [HCO-3] in the lumen must be due to changes in active HCO-3 (H+) transport. Thus, active H+ ion secretion across the luminal membrane of the proximal tubule is gradient dependent. </jats:p> |
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| author | Chan, Y. L., Malnic, G., Giebisch, G. |
| author_facet | Chan, Y. L., Malnic, G., Giebisch, G., Chan, Y. L., Malnic, G., Giebisch, G. |
| author_sort | chan, y. l. |
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| container_start_page | 0 |
| container_title | American Journal of Physiology-Renal Physiology |
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| description | <jats:p> The effect of oncotic pressure changes on fluid (Jv) and net bicarbonate transport (JHCO-3) and the transepithelial bicarbonate permeability (PHCO-3) were measured by an improved luminal and capillary microperfusion method that allows paired experiments on the same tubule. Rat proximal tubules were pump-perfused and Jv and [HCO-3] measured with [14C]inulin and a pH glass electrode. Raising peritubular protein (0-8-15 g/100 ml bovine serum albumin) stimulated Jv and HCO-3 reabsorption. The response to oncotic pressure changes was asymmetrical since changes of the luminal protein concentration had no significant effects. Whereas transepithelial solvent drag effects on HCO-3 must be minimal, peritubular protein most likely stimulates translocation of fluid and bicarbonate from intercellular spaces into peritubular capillaries. PHCO-3 was measured from HCO-3 net flux along a lumen-to-capillary-directed electrochemical potential gradient. In these experiments active H+ transport and Jv were minimized by 10(-4) M acetazolamide and luminal raffinose. PHCO-3 was 1.77 X 10(-5) cm X s-1 and was unaffected by increasing luminal flow rate from 10 to 45 nl X min-1. Since bicarbonate backflux is only a small fraction of physiological rates of JHCO-3, net transport alterations at varying [HCO-3] in the lumen must be due to changes in active HCO-3 (H+) transport. Thus, active H+ ion secretion across the luminal membrane of the proximal tubule is gradient dependent. </jats:p> |
| doi_str_mv | 10.1152/ajprenal.1983.245.5.f622 |
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| imprint | American Physiological Society, 1983 |
| imprint_str_mv | American Physiological Society, 1983 |
| institution | DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
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| issn_str_mv | 1931-857X, 1522-1466 |
| language | English |
| last_indexed | 2024-03-01T11:21:48.677Z |
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| physical | F622-F633 |
| publishDate | 1983 |
| publishDateSort | 1983 |
| publisher | American Physiological Society |
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| recordtype | ai |
| series | American Journal of Physiology-Renal Physiology |
| source_id | 49 |
| spelling | Chan, Y. L. Malnic, G. Giebisch, G. 1931-857X 1522-1466 American Physiological Society Physiology http://dx.doi.org/10.1152/ajprenal.1983.245.5.f622 <jats:p> The effect of oncotic pressure changes on fluid (Jv) and net bicarbonate transport (JHCO-3) and the transepithelial bicarbonate permeability (PHCO-3) were measured by an improved luminal and capillary microperfusion method that allows paired experiments on the same tubule. Rat proximal tubules were pump-perfused and Jv and [HCO-3] measured with [14C]inulin and a pH glass electrode. Raising peritubular protein (0-8-15 g/100 ml bovine serum albumin) stimulated Jv and HCO-3 reabsorption. The response to oncotic pressure changes was asymmetrical since changes of the luminal protein concentration had no significant effects. Whereas transepithelial solvent drag effects on HCO-3 must be minimal, peritubular protein most likely stimulates translocation of fluid and bicarbonate from intercellular spaces into peritubular capillaries. PHCO-3 was measured from HCO-3 net flux along a lumen-to-capillary-directed electrochemical potential gradient. In these experiments active H+ transport and Jv were minimized by 10(-4) M acetazolamide and luminal raffinose. PHCO-3 was 1.77 X 10(-5) cm X s-1 and was unaffected by increasing luminal flow rate from 10 to 45 nl X min-1. Since bicarbonate backflux is only a small fraction of physiological rates of JHCO-3, net transport alterations at varying [HCO-3] in the lumen must be due to changes in active HCO-3 (H+) transport. Thus, active H+ ion secretion across the luminal membrane of the proximal tubule is gradient dependent. </jats:p> Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion American Journal of Physiology-Renal Physiology |
| spellingShingle | Chan, Y. L., Malnic, G., Giebisch, G., American Journal of Physiology-Renal Physiology, Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion, Physiology |
| title | Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion |
| title_full | Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion |
| title_fullStr | Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion |
| title_full_unstemmed | Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion |
| title_short | Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion |
| title_sort | passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of h+ secretion |
| title_unstemmed | Passive driving forces of proximal tubular fluid and bicarbonate transport: gradient dependence of H+ secretion |
| topic | Physiology |
| url | http://dx.doi.org/10.1152/ajprenal.1983.245.5.f622 |