Eintrag weiter verarbeiten
To what extent is microtubular network involved in antidiuretic response?
Gespeichert in:
| Zeitschriftentitel: | American Journal of Physiology-Renal Physiology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | American Journal of Physiology-Renal Physiology, 255, 1988, 6, S. F1098-F1106 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
|
| Schlagwörter: |
| author_facet |
Valenti, G. Hugon, J. S. Bourguet, J. Valenti, G. Hugon, J. S. Bourguet, J. |
|---|---|
| author |
Valenti, G. Hugon, J. S. Bourguet, J. |
| spellingShingle |
Valenti, G. Hugon, J. S. Bourguet, J. American Journal of Physiology-Renal Physiology To what extent is microtubular network involved in antidiuretic response? Physiology |
| author_sort |
valenti, g. |
| spelling |
Valenti, G. Hugon, J. S. Bourguet, J. 1931-857X 1522-1466 American Physiological Society Physiology http://dx.doi.org/10.1152/ajprenal.1988.255.6.f1098 <jats:p> Antimitotic drugs markedly interfere with antidiuretic response, strongly implying that cytoskeleton integrity is essential to this function. This role of the cytoskeleton in controlling the epithelial transport has been seen as a necessary step in the translocation of the water channel containing particle aggregates and in their delivery to the apical membrane. We have now reexamined the exact role of the microtubular network by appropriate time course determinations, by the use of microtubule disruptive agents that lack of the side effects of colchicine, and by trying to visualize the apparent modifications of the microtubular network that accompany water permeability alterations using immunocytochemical techniques. Our results fully confirm that after microtubular network disruption, antidiuretic hormone-induced water permeability variations undergo typical alterations consisting in both a reduction in peak net water flow and a slowing down of its onset. At the same time, the microtubular network disappears in all the epithelial cells. We also show that colchicine-induced inhibition can still be observed in the presence of a prostaglandin synthetase inhibitor and that this inhibition is most likely to occur at a post-adenosine 3',5'-cyclic monophosphate level. These data, as well as results from other series with nocodazole, indicate that the reduction of the net water flow directly results from microtubular network disruption and not from side effects of the disrupting drugs. They also show that the hydrosmotic response is only partially dependent on the microtubular network, which probably has only a guidance role in the translocation of particle aggregates and their exocytotic fusion to the apical membrane. </jats:p> To what extent is microtubular network involved in antidiuretic response? American Journal of Physiology-Renal Physiology |
| doi_str_mv |
10.1152/ajprenal.1988.255.6.f1098 |
| facet_avail |
Online |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1Mi9hanByZW5hbC4xOTg4LjI1NS42LmYxMDk4 |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1Mi9hanByZW5hbC4xOTg4LjI1NS42LmYxMDk4 |
| institution |
DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-14 |
| imprint |
American Physiological Society, 1988 |
| imprint_str_mv |
American Physiological Society, 1988 |
| issn |
1522-1466 1931-857X |
| issn_str_mv |
1522-1466 1931-857X |
| language |
English |
| mega_collection |
American Physiological Society (CrossRef) |
| match_str |
valenti1988towhatextentismicrotubularnetworkinvolvedinantidiureticresponse |
| publishDateSort |
1988 |
| publisher |
American Physiological Society |
| recordtype |
ai |
| record_format |
ai |
| series |
American Journal of Physiology-Renal Physiology |
| source_id |
49 |
| title |
To what extent is microtubular network involved in antidiuretic response? |
| title_unstemmed |
To what extent is microtubular network involved in antidiuretic response? |
| title_full |
To what extent is microtubular network involved in antidiuretic response? |
| title_fullStr |
To what extent is microtubular network involved in antidiuretic response? |
| title_full_unstemmed |
To what extent is microtubular network involved in antidiuretic response? |
| title_short |
To what extent is microtubular network involved in antidiuretic response? |
| title_sort |
to what extent is microtubular network involved in antidiuretic response? |
| topic |
Physiology |
| url |
http://dx.doi.org/10.1152/ajprenal.1988.255.6.f1098 |
| publishDate |
1988 |
| physical |
F1098-F1106 |
| description |
<jats:p> Antimitotic drugs markedly interfere with antidiuretic response, strongly implying that cytoskeleton integrity is essential to this function. This role of the cytoskeleton in controlling the epithelial transport has been seen as a necessary step in the translocation of the water channel containing particle aggregates and in their delivery to the apical membrane. We have now reexamined the exact role of the microtubular network by appropriate time course determinations, by the use of microtubule disruptive agents that lack of the side effects of colchicine, and by trying to visualize the apparent modifications of the microtubular network that accompany water permeability alterations using immunocytochemical techniques. Our results fully confirm that after microtubular network disruption, antidiuretic hormone-induced water permeability variations undergo typical alterations consisting in both a reduction in peak net water flow and a slowing down of its onset. At the same time, the microtubular network disappears in all the epithelial cells. We also show that colchicine-induced inhibition can still be observed in the presence of a prostaglandin synthetase inhibitor and that this inhibition is most likely to occur at a post-adenosine 3',5'-cyclic monophosphate level. These data, as well as results from other series with nocodazole, indicate that the reduction of the net water flow directly results from microtubular network disruption and not from side effects of the disrupting drugs. They also show that the hydrosmotic response is only partially dependent on the microtubular network, which probably has only a guidance role in the translocation of particle aggregates and their exocytotic fusion to the apical membrane. </jats:p> |
| container_issue |
6 |
| container_start_page |
0 |
| container_title |
American Journal of Physiology-Renal Physiology |
| container_volume |
255 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792329342091526144 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T13:06:34.9Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=To+what+extent+is+microtubular+network+involved+in+antidiuretic+response%3F&rft.date=1988-12-01&genre=article&issn=1522-1466&volume=255&issue=6&pages=F1098-F1106&jtitle=American+Journal+of+Physiology-Renal+Physiology&atitle=To+what+extent+is+microtubular+network+involved+in+antidiuretic+response%3F&aulast=Bourguet&aufirst=J.&rft_id=info%3Adoi%2F10.1152%2Fajprenal.1988.255.6.f1098&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792329342091526144 |
| author | Valenti, G., Hugon, J. S., Bourguet, J. |
| author_facet | Valenti, G., Hugon, J. S., Bourguet, J., Valenti, G., Hugon, J. S., Bourguet, J. |
| author_sort | valenti, g. |
| container_issue | 6 |
| container_start_page | 0 |
| container_title | American Journal of Physiology-Renal Physiology |
| container_volume | 255 |
| description | <jats:p> Antimitotic drugs markedly interfere with antidiuretic response, strongly implying that cytoskeleton integrity is essential to this function. This role of the cytoskeleton in controlling the epithelial transport has been seen as a necessary step in the translocation of the water channel containing particle aggregates and in their delivery to the apical membrane. We have now reexamined the exact role of the microtubular network by appropriate time course determinations, by the use of microtubule disruptive agents that lack of the side effects of colchicine, and by trying to visualize the apparent modifications of the microtubular network that accompany water permeability alterations using immunocytochemical techniques. Our results fully confirm that after microtubular network disruption, antidiuretic hormone-induced water permeability variations undergo typical alterations consisting in both a reduction in peak net water flow and a slowing down of its onset. At the same time, the microtubular network disappears in all the epithelial cells. We also show that colchicine-induced inhibition can still be observed in the presence of a prostaglandin synthetase inhibitor and that this inhibition is most likely to occur at a post-adenosine 3',5'-cyclic monophosphate level. These data, as well as results from other series with nocodazole, indicate that the reduction of the net water flow directly results from microtubular network disruption and not from side effects of the disrupting drugs. They also show that the hydrosmotic response is only partially dependent on the microtubular network, which probably has only a guidance role in the translocation of particle aggregates and their exocytotic fusion to the apical membrane. </jats:p> |
| doi_str_mv | 10.1152/ajprenal.1988.255.6.f1098 |
| facet_avail | Online |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1Mi9hanByZW5hbC4xOTg4LjI1NS42LmYxMDk4 |
| imprint | American Physiological Society, 1988 |
| imprint_str_mv | American Physiological Society, 1988 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-14 |
| issn | 1522-1466, 1931-857X |
| issn_str_mv | 1522-1466, 1931-857X |
| language | English |
| last_indexed | 2024-03-01T13:06:34.9Z |
| match_str | valenti1988towhatextentismicrotubularnetworkinvolvedinantidiureticresponse |
| mega_collection | American Physiological Society (CrossRef) |
| physical | F1098-F1106 |
| publishDate | 1988 |
| publishDateSort | 1988 |
| publisher | American Physiological Society |
| record_format | ai |
| recordtype | ai |
| series | American Journal of Physiology-Renal Physiology |
| source_id | 49 |
| spelling | Valenti, G. Hugon, J. S. Bourguet, J. 1931-857X 1522-1466 American Physiological Society Physiology http://dx.doi.org/10.1152/ajprenal.1988.255.6.f1098 <jats:p> Antimitotic drugs markedly interfere with antidiuretic response, strongly implying that cytoskeleton integrity is essential to this function. This role of the cytoskeleton in controlling the epithelial transport has been seen as a necessary step in the translocation of the water channel containing particle aggregates and in their delivery to the apical membrane. We have now reexamined the exact role of the microtubular network by appropriate time course determinations, by the use of microtubule disruptive agents that lack of the side effects of colchicine, and by trying to visualize the apparent modifications of the microtubular network that accompany water permeability alterations using immunocytochemical techniques. Our results fully confirm that after microtubular network disruption, antidiuretic hormone-induced water permeability variations undergo typical alterations consisting in both a reduction in peak net water flow and a slowing down of its onset. At the same time, the microtubular network disappears in all the epithelial cells. We also show that colchicine-induced inhibition can still be observed in the presence of a prostaglandin synthetase inhibitor and that this inhibition is most likely to occur at a post-adenosine 3',5'-cyclic monophosphate level. These data, as well as results from other series with nocodazole, indicate that the reduction of the net water flow directly results from microtubular network disruption and not from side effects of the disrupting drugs. They also show that the hydrosmotic response is only partially dependent on the microtubular network, which probably has only a guidance role in the translocation of particle aggregates and their exocytotic fusion to the apical membrane. </jats:p> To what extent is microtubular network involved in antidiuretic response? American Journal of Physiology-Renal Physiology |
| spellingShingle | Valenti, G., Hugon, J. S., Bourguet, J., American Journal of Physiology-Renal Physiology, To what extent is microtubular network involved in antidiuretic response?, Physiology |
| title | To what extent is microtubular network involved in antidiuretic response? |
| title_full | To what extent is microtubular network involved in antidiuretic response? |
| title_fullStr | To what extent is microtubular network involved in antidiuretic response? |
| title_full_unstemmed | To what extent is microtubular network involved in antidiuretic response? |
| title_short | To what extent is microtubular network involved in antidiuretic response? |
| title_sort | to what extent is microtubular network involved in antidiuretic response? |
| title_unstemmed | To what extent is microtubular network involved in antidiuretic response? |
| topic | Physiology |
| url | http://dx.doi.org/10.1152/ajprenal.1988.255.6.f1098 |