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Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension
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| Zeitschriftentitel: | American Journal of Physiology-Lung Cellular and Molecular Physiology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , |
| In: | American Journal of Physiology-Lung Cellular and Molecular Physiology, 314, 2018, 1, S. L118-L126 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
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| Schlagwörter: |
| author_facet |
Suzuki, Toshio Carrier, Erica J. Talati, Megha H. Rathinasabapathy, Anandharajan Chen, Xinping Nishimura, Rintaro Tada, Yuji Tatsumi, Koichiro West, James Suzuki, Toshio Carrier, Erica J. Talati, Megha H. Rathinasabapathy, Anandharajan Chen, Xinping Nishimura, Rintaro Tada, Yuji Tatsumi, Koichiro West, James |
|---|---|
| author |
Suzuki, Toshio Carrier, Erica J. Talati, Megha H. Rathinasabapathy, Anandharajan Chen, Xinping Nishimura, Rintaro Tada, Yuji Tatsumi, Koichiro West, James |
| spellingShingle |
Suzuki, Toshio Carrier, Erica J. Talati, Megha H. Rathinasabapathy, Anandharajan Chen, Xinping Nishimura, Rintaro Tada, Yuji Tatsumi, Koichiro West, James American Journal of Physiology-Lung Cellular and Molecular Physiology Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension Cell Biology Physiology (medical) Pulmonary and Respiratory Medicine Physiology |
| author_sort |
suzuki, toshio |
| spelling |
Suzuki, Toshio Carrier, Erica J. Talati, Megha H. Rathinasabapathy, Anandharajan Chen, Xinping Nishimura, Rintaro Tada, Yuji Tatsumi, Koichiro West, James 1040-0605 1522-1504 American Physiological Society Cell Biology Physiology (medical) Pulmonary and Respiratory Medicine Physiology http://dx.doi.org/10.1152/ajplung.00296.2017 <jats:p> Endothelial-to-mesenchymal transition (EndMT) is a process in which endothelial cells lose polarity and cell-to cell contacts, and undergo a dramatic remodeling of the cytoskeleton. It has been implicated in initiation and progression of pulmonary arterial hypertension (PAH). However, the characteristics of cells which have undergone EndMT cells in vivo have not been reported and so remain unclear. To study this, sugen5416 and hypoxia (SuHx)-induced PAH was established in Cdh5-Cre/Gt(ROSA)26Sor<jats:sup>tm4(ACTB-tdTomato,EGFP)Luo</jats:sup>/J double transgenic mice, in which GFP was stably expressed in pan-endothelial cells. After 3 wk of SuHx, flow cytometry and immunohistochemistry demonstrated CD144-negative and GFP-positive cells (complete EndMT cells) possessed higher proliferative and migratory activity compared with other mesenchymal cells. While CD144-positive and α-smooth muscle actin (α-SMA)-positive cells (partial EndMT cells) continued to express endothelial progenitor cell markers, complete EndMT cells were Sca-1-rich mesenchymal cells with high proliferative and migratory ability. When transferred in fibronectin-coated chamber slides containing smooth muscle media, α-SMA robustly expressed in these cells compared with cEndMT cells that were grown in maintenance media. Demonstrating additional paracrine effects, conditioned medium from isolated complete EndMT cells induced enhanced mesenchymal proliferation and migration and increased angiogenesis compared with conditioned medium from resident mesenchymal cells. Overall, these findings show that EndMT cells could contribute to the pathogenesis of PAH both directly, by transformation into smooth muscle-like cells with higher proliferative and migratory potency, and indirectly, through paracrine effects on vascular intimal and medial proliferation. </jats:p> Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension American Journal of Physiology-Lung Cellular and Molecular Physiology |
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| title |
Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| title_unstemmed |
Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| title_full |
Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| title_fullStr |
Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| title_full_unstemmed |
Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| title_short |
Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| title_sort |
isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| topic |
Cell Biology Physiology (medical) Pulmonary and Respiratory Medicine Physiology |
| url |
http://dx.doi.org/10.1152/ajplung.00296.2017 |
| publishDate |
2018 |
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L118-L126 |
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<jats:p> Endothelial-to-mesenchymal transition (EndMT) is a process in which endothelial cells lose polarity and cell-to cell contacts, and undergo a dramatic remodeling of the cytoskeleton. It has been implicated in initiation and progression of pulmonary arterial hypertension (PAH). However, the characteristics of cells which have undergone EndMT cells in vivo have not been reported and so remain unclear. To study this, sugen5416 and hypoxia (SuHx)-induced PAH was established in Cdh5-Cre/Gt(ROSA)26Sor<jats:sup>tm4(ACTB-tdTomato,EGFP)Luo</jats:sup>/J double transgenic mice, in which GFP was stably expressed in pan-endothelial cells. After 3 wk of SuHx, flow cytometry and immunohistochemistry demonstrated CD144-negative and GFP-positive cells (complete EndMT cells) possessed higher proliferative and migratory activity compared with other mesenchymal cells. While CD144-positive and α-smooth muscle actin (α-SMA)-positive cells (partial EndMT cells) continued to express endothelial progenitor cell markers, complete EndMT cells were Sca-1-rich mesenchymal cells with high proliferative and migratory ability. When transferred in fibronectin-coated chamber slides containing smooth muscle media, α-SMA robustly expressed in these cells compared with cEndMT cells that were grown in maintenance media. Demonstrating additional paracrine effects, conditioned medium from isolated complete EndMT cells induced enhanced mesenchymal proliferation and migration and increased angiogenesis compared with conditioned medium from resident mesenchymal cells. Overall, these findings show that EndMT cells could contribute to the pathogenesis of PAH both directly, by transformation into smooth muscle-like cells with higher proliferative and migratory potency, and indirectly, through paracrine effects on vascular intimal and medial proliferation. </jats:p> |
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| author | Suzuki, Toshio, Carrier, Erica J., Talati, Megha H., Rathinasabapathy, Anandharajan, Chen, Xinping, Nishimura, Rintaro, Tada, Yuji, Tatsumi, Koichiro, West, James |
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| description | <jats:p> Endothelial-to-mesenchymal transition (EndMT) is a process in which endothelial cells lose polarity and cell-to cell contacts, and undergo a dramatic remodeling of the cytoskeleton. It has been implicated in initiation and progression of pulmonary arterial hypertension (PAH). However, the characteristics of cells which have undergone EndMT cells in vivo have not been reported and so remain unclear. To study this, sugen5416 and hypoxia (SuHx)-induced PAH was established in Cdh5-Cre/Gt(ROSA)26Sor<jats:sup>tm4(ACTB-tdTomato,EGFP)Luo</jats:sup>/J double transgenic mice, in which GFP was stably expressed in pan-endothelial cells. After 3 wk of SuHx, flow cytometry and immunohistochemistry demonstrated CD144-negative and GFP-positive cells (complete EndMT cells) possessed higher proliferative and migratory activity compared with other mesenchymal cells. While CD144-positive and α-smooth muscle actin (α-SMA)-positive cells (partial EndMT cells) continued to express endothelial progenitor cell markers, complete EndMT cells were Sca-1-rich mesenchymal cells with high proliferative and migratory ability. When transferred in fibronectin-coated chamber slides containing smooth muscle media, α-SMA robustly expressed in these cells compared with cEndMT cells that were grown in maintenance media. Demonstrating additional paracrine effects, conditioned medium from isolated complete EndMT cells induced enhanced mesenchymal proliferation and migration and increased angiogenesis compared with conditioned medium from resident mesenchymal cells. Overall, these findings show that EndMT cells could contribute to the pathogenesis of PAH both directly, by transformation into smooth muscle-like cells with higher proliferative and migratory potency, and indirectly, through paracrine effects on vascular intimal and medial proliferation. </jats:p> |
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| spelling | Suzuki, Toshio Carrier, Erica J. Talati, Megha H. Rathinasabapathy, Anandharajan Chen, Xinping Nishimura, Rintaro Tada, Yuji Tatsumi, Koichiro West, James 1040-0605 1522-1504 American Physiological Society Cell Biology Physiology (medical) Pulmonary and Respiratory Medicine Physiology http://dx.doi.org/10.1152/ajplung.00296.2017 <jats:p> Endothelial-to-mesenchymal transition (EndMT) is a process in which endothelial cells lose polarity and cell-to cell contacts, and undergo a dramatic remodeling of the cytoskeleton. It has been implicated in initiation and progression of pulmonary arterial hypertension (PAH). However, the characteristics of cells which have undergone EndMT cells in vivo have not been reported and so remain unclear. To study this, sugen5416 and hypoxia (SuHx)-induced PAH was established in Cdh5-Cre/Gt(ROSA)26Sor<jats:sup>tm4(ACTB-tdTomato,EGFP)Luo</jats:sup>/J double transgenic mice, in which GFP was stably expressed in pan-endothelial cells. After 3 wk of SuHx, flow cytometry and immunohistochemistry demonstrated CD144-negative and GFP-positive cells (complete EndMT cells) possessed higher proliferative and migratory activity compared with other mesenchymal cells. While CD144-positive and α-smooth muscle actin (α-SMA)-positive cells (partial EndMT cells) continued to express endothelial progenitor cell markers, complete EndMT cells were Sca-1-rich mesenchymal cells with high proliferative and migratory ability. When transferred in fibronectin-coated chamber slides containing smooth muscle media, α-SMA robustly expressed in these cells compared with cEndMT cells that were grown in maintenance media. Demonstrating additional paracrine effects, conditioned medium from isolated complete EndMT cells induced enhanced mesenchymal proliferation and migration and increased angiogenesis compared with conditioned medium from resident mesenchymal cells. Overall, these findings show that EndMT cells could contribute to the pathogenesis of PAH both directly, by transformation into smooth muscle-like cells with higher proliferative and migratory potency, and indirectly, through paracrine effects on vascular intimal and medial proliferation. </jats:p> Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension American Journal of Physiology-Lung Cellular and Molecular Physiology |
| spellingShingle | Suzuki, Toshio, Carrier, Erica J., Talati, Megha H., Rathinasabapathy, Anandharajan, Chen, Xinping, Nishimura, Rintaro, Tada, Yuji, Tatsumi, Koichiro, West, James, American Journal of Physiology-Lung Cellular and Molecular Physiology, Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension, Cell Biology, Physiology (medical), Pulmonary and Respiratory Medicine, Physiology |
| title | Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| title_full | Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| title_fullStr | Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| title_full_unstemmed | Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| title_short | Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| title_sort | isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| title_unstemmed | Isolation and characterization of endothelial-to-mesenchymal transition cells in pulmonary arterial hypertension |
| topic | Cell Biology, Physiology (medical), Pulmonary and Respiratory Medicine, Physiology |
| url | http://dx.doi.org/10.1152/ajplung.00296.2017 |