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Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells
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| Zeitschriftentitel: | American Journal of Physiology-Gastrointestinal and Liver Physiology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | American Journal of Physiology-Gastrointestinal and Liver Physiology, 292, 2007, 3, S. G711-G717 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
|
| Schlagwörter: |
| author_facet |
Bachmann, Oliver Heinzmann, Alexander Mack, Andreas Manns, Michael P. Seidler, Ursula Bachmann, Oliver Heinzmann, Alexander Mack, Andreas Manns, Michael P. Seidler, Ursula |
|---|---|
| author |
Bachmann, Oliver Heinzmann, Alexander Mack, Andreas Manns, Michael P. Seidler, Ursula |
| spellingShingle |
Bachmann, Oliver Heinzmann, Alexander Mack, Andreas Manns, Michael P. Seidler, Ursula American Journal of Physiology-Gastrointestinal and Liver Physiology Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells Physiology (medical) Gastroenterology Hepatology Physiology |
| author_sort |
bachmann, oliver |
| spelling |
Bachmann, Oliver Heinzmann, Alexander Mack, Andreas Manns, Michael P. Seidler, Ursula 0193-1857 1522-1547 American Physiological Society Physiology (medical) Gastroenterology Hepatology Physiology http://dx.doi.org/10.1152/ajpgi.00416.2006 <jats:p> We have previously shown that stimulation of acid secretion in parietal cells causes rapid initial cell shrinkage, followed by Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup> exchange-mediated regulatory volume increase (RVI). The factors leading to the initial cell shrinkage are unknown. We therefore monitored volume changes in cultured rabbit parietal cells by confocal measurement of the cytoplasmic calcein concentration. Although blocking the presumably apically located K<jats:sup>+</jats:sup> channel KCNQ1 with chromanol 293b reduced both the forskolin- and carbachol-induced cell shrinkage, inhibition of Ca<jats:sup>2+</jats:sup>-sensitive K<jats:sup>+</jats:sup> channels with charybdotoxin strongly inhibited the cell volume decrease after carbachol, but not after forskolin stimulation. The cell shrinkage induced by both secretagogues was partially inhibited by blocking H<jats:sup>+</jats:sup>-K<jats:sup>+</jats:sup>-ATPase with SCH28080 and completely absent after incubation with NPPB, which inhibits parietal cell anion conductances involved in acid secretion. The subsequent RVI was strongly inhibited with the Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup> exchanger 1 (NHE1)-specific concentration of HOE642 and completely by 500 μM dimethyl-amiloride (DMA), which also inhibits NHE4. None of the above substances induced volume changes under baseline conditions. Our results indicate that cell volume decrease associated with acid secretion is dependent on the activation of K<jats:sup>+</jats:sup> and Cl<jats:sup>−</jats:sup> channels by the respective secretagogues. K<jats:sup>+</jats:sup>, Cl<jats:sup>−</jats:sup>, and water secretion into the secretory canaliculi is thus one likely mechanism of stimulation-associated cell shrinkage in cultured parietal cells. The observed RVI is predominantly mediated by NHE1. </jats:p> Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells American Journal of Physiology-Gastrointestinal and Liver Physiology |
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| title |
Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| title_unstemmed |
Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| title_full |
Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| title_fullStr |
Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| title_full_unstemmed |
Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| title_short |
Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| title_sort |
mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| topic |
Physiology (medical) Gastroenterology Hepatology Physiology |
| url |
http://dx.doi.org/10.1152/ajpgi.00416.2006 |
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2007 |
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G711-G717 |
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<jats:p> We have previously shown that stimulation of acid secretion in parietal cells causes rapid initial cell shrinkage, followed by Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup> exchange-mediated regulatory volume increase (RVI). The factors leading to the initial cell shrinkage are unknown. We therefore monitored volume changes in cultured rabbit parietal cells by confocal measurement of the cytoplasmic calcein concentration. Although blocking the presumably apically located K<jats:sup>+</jats:sup> channel KCNQ1 with chromanol 293b reduced both the forskolin- and carbachol-induced cell shrinkage, inhibition of Ca<jats:sup>2+</jats:sup>-sensitive K<jats:sup>+</jats:sup> channels with charybdotoxin strongly inhibited the cell volume decrease after carbachol, but not after forskolin stimulation. The cell shrinkage induced by both secretagogues was partially inhibited by blocking H<jats:sup>+</jats:sup>-K<jats:sup>+</jats:sup>-ATPase with SCH28080 and completely absent after incubation with NPPB, which inhibits parietal cell anion conductances involved in acid secretion. The subsequent RVI was strongly inhibited with the Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup> exchanger 1 (NHE1)-specific concentration of HOE642 and completely by 500 μM dimethyl-amiloride (DMA), which also inhibits NHE4. None of the above substances induced volume changes under baseline conditions. Our results indicate that cell volume decrease associated with acid secretion is dependent on the activation of K<jats:sup>+</jats:sup> and Cl<jats:sup>−</jats:sup> channels by the respective secretagogues. K<jats:sup>+</jats:sup>, Cl<jats:sup>−</jats:sup>, and water secretion into the secretory canaliculi is thus one likely mechanism of stimulation-associated cell shrinkage in cultured parietal cells. The observed RVI is predominantly mediated by NHE1. </jats:p> |
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| author | Bachmann, Oliver, Heinzmann, Alexander, Mack, Andreas, Manns, Michael P., Seidler, Ursula |
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| description | <jats:p> We have previously shown that stimulation of acid secretion in parietal cells causes rapid initial cell shrinkage, followed by Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup> exchange-mediated regulatory volume increase (RVI). The factors leading to the initial cell shrinkage are unknown. We therefore monitored volume changes in cultured rabbit parietal cells by confocal measurement of the cytoplasmic calcein concentration. Although blocking the presumably apically located K<jats:sup>+</jats:sup> channel KCNQ1 with chromanol 293b reduced both the forskolin- and carbachol-induced cell shrinkage, inhibition of Ca<jats:sup>2+</jats:sup>-sensitive K<jats:sup>+</jats:sup> channels with charybdotoxin strongly inhibited the cell volume decrease after carbachol, but not after forskolin stimulation. The cell shrinkage induced by both secretagogues was partially inhibited by blocking H<jats:sup>+</jats:sup>-K<jats:sup>+</jats:sup>-ATPase with SCH28080 and completely absent after incubation with NPPB, which inhibits parietal cell anion conductances involved in acid secretion. The subsequent RVI was strongly inhibited with the Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup> exchanger 1 (NHE1)-specific concentration of HOE642 and completely by 500 μM dimethyl-amiloride (DMA), which also inhibits NHE4. None of the above substances induced volume changes under baseline conditions. Our results indicate that cell volume decrease associated with acid secretion is dependent on the activation of K<jats:sup>+</jats:sup> and Cl<jats:sup>−</jats:sup> channels by the respective secretagogues. K<jats:sup>+</jats:sup>, Cl<jats:sup>−</jats:sup>, and water secretion into the secretory canaliculi is thus one likely mechanism of stimulation-associated cell shrinkage in cultured parietal cells. The observed RVI is predominantly mediated by NHE1. </jats:p> |
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| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| spelling | Bachmann, Oliver Heinzmann, Alexander Mack, Andreas Manns, Michael P. Seidler, Ursula 0193-1857 1522-1547 American Physiological Society Physiology (medical) Gastroenterology Hepatology Physiology http://dx.doi.org/10.1152/ajpgi.00416.2006 <jats:p> We have previously shown that stimulation of acid secretion in parietal cells causes rapid initial cell shrinkage, followed by Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup> exchange-mediated regulatory volume increase (RVI). The factors leading to the initial cell shrinkage are unknown. We therefore monitored volume changes in cultured rabbit parietal cells by confocal measurement of the cytoplasmic calcein concentration. Although blocking the presumably apically located K<jats:sup>+</jats:sup> channel KCNQ1 with chromanol 293b reduced both the forskolin- and carbachol-induced cell shrinkage, inhibition of Ca<jats:sup>2+</jats:sup>-sensitive K<jats:sup>+</jats:sup> channels with charybdotoxin strongly inhibited the cell volume decrease after carbachol, but not after forskolin stimulation. The cell shrinkage induced by both secretagogues was partially inhibited by blocking H<jats:sup>+</jats:sup>-K<jats:sup>+</jats:sup>-ATPase with SCH28080 and completely absent after incubation with NPPB, which inhibits parietal cell anion conductances involved in acid secretion. The subsequent RVI was strongly inhibited with the Na<jats:sup>+</jats:sup>/H<jats:sup>+</jats:sup> exchanger 1 (NHE1)-specific concentration of HOE642 and completely by 500 μM dimethyl-amiloride (DMA), which also inhibits NHE4. None of the above substances induced volume changes under baseline conditions. Our results indicate that cell volume decrease associated with acid secretion is dependent on the activation of K<jats:sup>+</jats:sup> and Cl<jats:sup>−</jats:sup> channels by the respective secretagogues. K<jats:sup>+</jats:sup>, Cl<jats:sup>−</jats:sup>, and water secretion into the secretory canaliculi is thus one likely mechanism of stimulation-associated cell shrinkage in cultured parietal cells. The observed RVI is predominantly mediated by NHE1. </jats:p> Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells American Journal of Physiology-Gastrointestinal and Liver Physiology |
| spellingShingle | Bachmann, Oliver, Heinzmann, Alexander, Mack, Andreas, Manns, Michael P., Seidler, Ursula, American Journal of Physiology-Gastrointestinal and Liver Physiology, Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells, Physiology (medical), Gastroenterology, Hepatology, Physiology |
| title | Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| title_full | Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| title_fullStr | Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| title_full_unstemmed | Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| title_short | Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| title_sort | mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| title_unstemmed | Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cells |
| topic | Physiology (medical), Gastroenterology, Hepatology, Physiology |
| url | http://dx.doi.org/10.1152/ajpgi.00416.2006 |