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Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat
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| Zeitschriftentitel: | American Journal of Physiology-Cell Physiology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | American Journal of Physiology-Cell Physiology, 279, 2000, 3, S. C682-C690 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
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| Schlagwörter: |
| author_facet |
Putman, Charles T. Düsterhöft, Sabine Pette, Dirk Putman, Charles T. Düsterhöft, Sabine Pette, Dirk |
|---|---|
| author |
Putman, Charles T. Düsterhöft, Sabine Pette, Dirk |
| spellingShingle |
Putman, Charles T. Düsterhöft, Sabine Pette, Dirk American Journal of Physiology-Cell Physiology Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat Cell Biology Physiology |
| author_sort |
putman, charles t. |
| spelling |
Putman, Charles T. Düsterhöft, Sabine Pette, Dirk 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.2000.279.3.c682 <jats:p>Satellite cell proliferation was assessed in low-frequency-stimulated hypothyroid rat fast-twitch muscle by 5-bromo-2′-deoxyuridine (BrdU) labeling and subsequent staining of labeled muscle nuclei, and by staining for proliferating cell nuclear antigen (PCNA). BrdU labeling and PCNA staining were highly correlated and increased approximately fourfold at 5 days of stimulation, decayed thereafter, but remained elevated over control in 10- and 20-day stimulated muscles. Myogenin mRNA was ∼4-fold elevated at 5 days and 1.5-fold at 10 days. Staining for myogenin protein yielded results similar to that for PCNA and BrdU. Furthermore, a detailed examination of the pattern of myogenin staining revealed that the number of myogenin-positive nuclei was elevated in the fast pure IIB fiber population at 5 and 10 days of chronic low-frequency stimulation. By 20 days, myogenin staining was observed in transforming fast fibers that coexpressed embryonic and adult myosin heavy chain isoforms. In the slower fiber populations (i.e., IIA and I), myogenin-positive transforming fibers that coexpressed embryonic myosin heavy chain, appeared already at 5 days. Thus the satellite cell progeny on slower fibers seemed to proliferate less and to fuse earlier to their associated fibers than the satellite cell progeny on fast fibers. We suggest that the increase in muscle nuclei of the fast fibers might be a prerequisite for fast-to-slow fiber type transitions.</jats:p> Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat American Journal of Physiology-Cell Physiology |
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| title |
Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| title_unstemmed |
Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| title_full |
Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| title_fullStr |
Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| title_full_unstemmed |
Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| title_short |
Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| title_sort |
satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| topic |
Cell Biology Physiology |
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http://dx.doi.org/10.1152/ajpcell.2000.279.3.c682 |
| publishDate |
2000 |
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C682-C690 |
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<jats:p>Satellite cell proliferation was assessed in low-frequency-stimulated hypothyroid rat fast-twitch muscle by 5-bromo-2′-deoxyuridine (BrdU) labeling and subsequent staining of labeled muscle nuclei, and by staining for proliferating cell nuclear antigen (PCNA). BrdU labeling and PCNA staining were highly correlated and increased approximately fourfold at 5 days of stimulation, decayed thereafter, but remained elevated over control in 10- and 20-day stimulated muscles. Myogenin mRNA was ∼4-fold elevated at 5 days and 1.5-fold at 10 days. Staining for myogenin protein yielded results similar to that for PCNA and BrdU. Furthermore, a detailed examination of the pattern of myogenin staining revealed that the number of myogenin-positive nuclei was elevated in the fast pure IIB fiber population at 5 and 10 days of chronic low-frequency stimulation. By 20 days, myogenin staining was observed in transforming fast fibers that coexpressed embryonic and adult myosin heavy chain isoforms. In the slower fiber populations (i.e., IIA and I), myogenin-positive transforming fibers that coexpressed embryonic myosin heavy chain, appeared already at 5 days. Thus the satellite cell progeny on slower fibers seemed to proliferate less and to fuse earlier to their associated fibers than the satellite cell progeny on fast fibers. We suggest that the increase in muscle nuclei of the fast fibers might be a prerequisite for fast-to-slow fiber type transitions.</jats:p> |
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| author | Putman, Charles T., Düsterhöft, Sabine, Pette, Dirk |
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| description | <jats:p>Satellite cell proliferation was assessed in low-frequency-stimulated hypothyroid rat fast-twitch muscle by 5-bromo-2′-deoxyuridine (BrdU) labeling and subsequent staining of labeled muscle nuclei, and by staining for proliferating cell nuclear antigen (PCNA). BrdU labeling and PCNA staining were highly correlated and increased approximately fourfold at 5 days of stimulation, decayed thereafter, but remained elevated over control in 10- and 20-day stimulated muscles. Myogenin mRNA was ∼4-fold elevated at 5 days and 1.5-fold at 10 days. Staining for myogenin protein yielded results similar to that for PCNA and BrdU. Furthermore, a detailed examination of the pattern of myogenin staining revealed that the number of myogenin-positive nuclei was elevated in the fast pure IIB fiber population at 5 and 10 days of chronic low-frequency stimulation. By 20 days, myogenin staining was observed in transforming fast fibers that coexpressed embryonic and adult myosin heavy chain isoforms. In the slower fiber populations (i.e., IIA and I), myogenin-positive transforming fibers that coexpressed embryonic myosin heavy chain, appeared already at 5 days. Thus the satellite cell progeny on slower fibers seemed to proliferate less and to fuse earlier to their associated fibers than the satellite cell progeny on fast fibers. We suggest that the increase in muscle nuclei of the fast fibers might be a prerequisite for fast-to-slow fiber type transitions.</jats:p> |
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| spelling | Putman, Charles T. Düsterhöft, Sabine Pette, Dirk 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.2000.279.3.c682 <jats:p>Satellite cell proliferation was assessed in low-frequency-stimulated hypothyroid rat fast-twitch muscle by 5-bromo-2′-deoxyuridine (BrdU) labeling and subsequent staining of labeled muscle nuclei, and by staining for proliferating cell nuclear antigen (PCNA). BrdU labeling and PCNA staining were highly correlated and increased approximately fourfold at 5 days of stimulation, decayed thereafter, but remained elevated over control in 10- and 20-day stimulated muscles. Myogenin mRNA was ∼4-fold elevated at 5 days and 1.5-fold at 10 days. Staining for myogenin protein yielded results similar to that for PCNA and BrdU. Furthermore, a detailed examination of the pattern of myogenin staining revealed that the number of myogenin-positive nuclei was elevated in the fast pure IIB fiber population at 5 and 10 days of chronic low-frequency stimulation. By 20 days, myogenin staining was observed in transforming fast fibers that coexpressed embryonic and adult myosin heavy chain isoforms. In the slower fiber populations (i.e., IIA and I), myogenin-positive transforming fibers that coexpressed embryonic myosin heavy chain, appeared already at 5 days. Thus the satellite cell progeny on slower fibers seemed to proliferate less and to fuse earlier to their associated fibers than the satellite cell progeny on fast fibers. We suggest that the increase in muscle nuclei of the fast fibers might be a prerequisite for fast-to-slow fiber type transitions.</jats:p> Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat American Journal of Physiology-Cell Physiology |
| spellingShingle | Putman, Charles T., Düsterhöft, Sabine, Pette, Dirk, American Journal of Physiology-Cell Physiology, Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat, Cell Biology, Physiology |
| title | Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| title_full | Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| title_fullStr | Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| title_full_unstemmed | Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| title_short | Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| title_sort | satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| title_unstemmed | Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat |
| topic | Cell Biology, Physiology |
| url | http://dx.doi.org/10.1152/ajpcell.2000.279.3.c682 |