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Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells
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| Zeitschriftentitel: | American Journal of Physiology-Cell Physiology |
|---|---|
| Personen und Körperschaften: | , |
| In: | American Journal of Physiology-Cell Physiology, 259, 1990, 4, S. C647-C653 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
|
| Schlagwörter: |
| author_facet |
Lundgren, D. W. Vacca, C. V. Lundgren, D. W. Vacca, C. V. |
|---|---|
| author |
Lundgren, D. W. Vacca, C. V. |
| spellingShingle |
Lundgren, D. W. Vacca, C. V. American Journal of Physiology-Cell Physiology Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells Cell Biology Physiology |
| author_sort |
lundgren, d. w. |
| spelling |
Lundgren, D. W. Vacca, C. V. 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.1990.259.4.c647 <jats:p>This report examines the effect of nonmetabolizable glucose analogues on ornithine decarboxylase (ODC) activity in LLC-PK1 cells. The addition of Na(+)-dependent cotransported glucose analogues, 1-O-methyl-alpha-D-glucopyranoside (alpha-MDG) and 1-O-methyl-beta-D-glucopyranoside, to Earle's balanced salt solution minus glucose (EBSS-G) increased ODC activity five- to sevenfold above basal levels. The passive carrier-mediated transported glucose analogue 3-O-methyl-D-glucopyranose had very little effect on enzyme activity. alpha-MDG increased ODC activity in quiescent but not growing cells. ODC activity increased as a function of both the incubation time in EBSS-G + alpha-MDG and the concentration of alpha-MDG in EBSS-G. Phlorizin significantly reduced the level of enzyme activity induced by alpha-MDG. ODC expression by alpha-MDG was reduced in cells incubated in hypertonic EBSS-G + alpha-MDG. Enzyme activity, in the absence of extracellular organic substrates, was markedly elevated in cells incubated in hypotonic media. It is suggested that an influx of Na+ and/or an increase in cell volume elevates one or more signal transducers that regulate ODC expression.</jats:p> Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells American Journal of Physiology-Cell Physiology |
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American Journal of Physiology-Cell Physiology |
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| title |
Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells |
| title_unstemmed |
Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells |
| title_full |
Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells |
| title_fullStr |
Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells |
| title_full_unstemmed |
Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells |
| title_short |
Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells |
| title_sort |
nonmetabolizable glucose analogues and ornithine decarboxylase expression in llc-pk1 cells |
| topic |
Cell Biology Physiology |
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http://dx.doi.org/10.1152/ajpcell.1990.259.4.c647 |
| publishDate |
1990 |
| physical |
C647-C653 |
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<jats:p>This report examines the effect of nonmetabolizable glucose analogues on ornithine decarboxylase (ODC) activity in LLC-PK1 cells. The addition of Na(+)-dependent cotransported glucose analogues, 1-O-methyl-alpha-D-glucopyranoside (alpha-MDG) and 1-O-methyl-beta-D-glucopyranoside, to Earle's balanced salt solution minus glucose (EBSS-G) increased ODC activity five- to sevenfold above basal levels. The passive carrier-mediated transported glucose analogue 3-O-methyl-D-glucopyranose had very little effect on enzyme activity. alpha-MDG increased ODC activity in quiescent but not growing cells. ODC activity increased as a function of both the incubation time in EBSS-G + alpha-MDG and the concentration of alpha-MDG in EBSS-G. Phlorizin significantly reduced the level of enzyme activity induced by alpha-MDG. ODC expression by alpha-MDG was reduced in cells incubated in hypertonic EBSS-G + alpha-MDG. Enzyme activity, in the absence of extracellular organic substrates, was markedly elevated in cells incubated in hypotonic media. It is suggested that an influx of Na+ and/or an increase in cell volume elevates one or more signal transducers that regulate ODC expression.</jats:p> |
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| author | Lundgren, D. W., Vacca, C. V. |
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| description | <jats:p>This report examines the effect of nonmetabolizable glucose analogues on ornithine decarboxylase (ODC) activity in LLC-PK1 cells. The addition of Na(+)-dependent cotransported glucose analogues, 1-O-methyl-alpha-D-glucopyranoside (alpha-MDG) and 1-O-methyl-beta-D-glucopyranoside, to Earle's balanced salt solution minus glucose (EBSS-G) increased ODC activity five- to sevenfold above basal levels. The passive carrier-mediated transported glucose analogue 3-O-methyl-D-glucopyranose had very little effect on enzyme activity. alpha-MDG increased ODC activity in quiescent but not growing cells. ODC activity increased as a function of both the incubation time in EBSS-G + alpha-MDG and the concentration of alpha-MDG in EBSS-G. Phlorizin significantly reduced the level of enzyme activity induced by alpha-MDG. ODC expression by alpha-MDG was reduced in cells incubated in hypertonic EBSS-G + alpha-MDG. Enzyme activity, in the absence of extracellular organic substrates, was markedly elevated in cells incubated in hypotonic media. It is suggested that an influx of Na+ and/or an increase in cell volume elevates one or more signal transducers that regulate ODC expression.</jats:p> |
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| imprint_str_mv | American Physiological Society, 1990 |
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| series | American Journal of Physiology-Cell Physiology |
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| spelling | Lundgren, D. W. Vacca, C. V. 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.1990.259.4.c647 <jats:p>This report examines the effect of nonmetabolizable glucose analogues on ornithine decarboxylase (ODC) activity in LLC-PK1 cells. The addition of Na(+)-dependent cotransported glucose analogues, 1-O-methyl-alpha-D-glucopyranoside (alpha-MDG) and 1-O-methyl-beta-D-glucopyranoside, to Earle's balanced salt solution minus glucose (EBSS-G) increased ODC activity five- to sevenfold above basal levels. The passive carrier-mediated transported glucose analogue 3-O-methyl-D-glucopyranose had very little effect on enzyme activity. alpha-MDG increased ODC activity in quiescent but not growing cells. ODC activity increased as a function of both the incubation time in EBSS-G + alpha-MDG and the concentration of alpha-MDG in EBSS-G. Phlorizin significantly reduced the level of enzyme activity induced by alpha-MDG. ODC expression by alpha-MDG was reduced in cells incubated in hypertonic EBSS-G + alpha-MDG. Enzyme activity, in the absence of extracellular organic substrates, was markedly elevated in cells incubated in hypotonic media. It is suggested that an influx of Na+ and/or an increase in cell volume elevates one or more signal transducers that regulate ODC expression.</jats:p> Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells American Journal of Physiology-Cell Physiology |
| spellingShingle | Lundgren, D. W., Vacca, C. V., American Journal of Physiology-Cell Physiology, Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells, Cell Biology, Physiology |
| title | Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells |
| title_full | Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells |
| title_fullStr | Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells |
| title_full_unstemmed | Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells |
| title_short | Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells |
| title_sort | nonmetabolizable glucose analogues and ornithine decarboxylase expression in llc-pk1 cells |
| title_unstemmed | Nonmetabolizable glucose analogues and ornithine decarboxylase expression in LLC-PK1 cells |
| topic | Cell Biology, Physiology |
| url | http://dx.doi.org/10.1152/ajpcell.1990.259.4.c647 |