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Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor
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| Zeitschriftentitel: | American Journal of Physiology-Cell Physiology |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | American Journal of Physiology-Cell Physiology, 259, 1990, 3, S. C427-C431 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
|
| Schlagwörter: |
| author_facet |
Ueno, M. Rondon, I. Beckman, B. Brookins, J. Nakashima, J. Cole, F. E. Fisher, J. W. Ueno, M. Rondon, I. Beckman, B. Brookins, J. Nakashima, J. Cole, F. E. Fisher, J. W. |
|---|---|
| author |
Ueno, M. Rondon, I. Beckman, B. Brookins, J. Nakashima, J. Cole, F. E. Fisher, J. W. |
| spellingShingle |
Ueno, M. Rondon, I. Beckman, B. Brookins, J. Nakashima, J. Cole, F. E. Fisher, J. W. American Journal of Physiology-Cell Physiology Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor Cell Biology Physiology |
| author_sort |
ueno, m. |
| spelling |
Ueno, M. Rondon, I. Beckman, B. Brookins, J. Nakashima, J. Cole, F. E. Fisher, J. W. 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.1990.259.3.c427 <jats:p>The present studies were undertaken to assess the effects of atrial natriuretic factor (ANF) on erythropoietin (Ep) secretion in Ep-producing renal carcinoma (RC) cells using a sensitive radioimmunoassay for Ep. Human ANF produced a significant dose-related increase in Ep secretion at concentrations of 10(-7) and 10(-6) M when compared with vehicle controls. ANF (greater than or equal to 10(-9) M) also significantly increased the intracellular guanosine 3',5'-cyclic monophosphate (cGMP) concentration after 5-min incubation with the RC cells. Scatchard analysis of the human 125I-labeled ANF binding data indicated that the RC cells contain a single class of binding sites with a dissociation constant (Kd) of 93 +/- 1 pM and a binding capacity of 2,190 +/- 750 sites/cell. Incubation of the RC cells with 8-bromo-cGMP in concentrations of 10(-7)-10(-5) M also produced a significant dose-related enhancement of Ep secretion. These findings suggest that the increase in Ep secretion in response to ANF can be attributed, at least in part, to activation of guanylate cyclase, which is coupled to specific ANF receptors on the RC cell.</jats:p> Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor American Journal of Physiology-Cell Physiology |
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American Journal of Physiology-Cell Physiology |
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| title |
Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| title_unstemmed |
Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| title_full |
Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| title_fullStr |
Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| title_full_unstemmed |
Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| title_short |
Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| title_sort |
increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| topic |
Cell Biology Physiology |
| url |
http://dx.doi.org/10.1152/ajpcell.1990.259.3.c427 |
| publishDate |
1990 |
| physical |
C427-C431 |
| description |
<jats:p>The present studies were undertaken to assess the effects of atrial natriuretic factor (ANF) on erythropoietin (Ep) secretion in Ep-producing renal carcinoma (RC) cells using a sensitive radioimmunoassay for Ep. Human ANF produced a significant dose-related increase in Ep secretion at concentrations of 10(-7) and 10(-6) M when compared with vehicle controls. ANF (greater than or equal to 10(-9) M) also significantly increased the intracellular guanosine 3',5'-cyclic monophosphate (cGMP) concentration after 5-min incubation with the RC cells. Scatchard analysis of the human 125I-labeled ANF binding data indicated that the RC cells contain a single class of binding sites with a dissociation constant (Kd) of 93 +/- 1 pM and a binding capacity of 2,190 +/- 750 sites/cell. Incubation of the RC cells with 8-bromo-cGMP in concentrations of 10(-7)-10(-5) M also produced a significant dose-related enhancement of Ep secretion. These findings suggest that the increase in Ep secretion in response to ANF can be attributed, at least in part, to activation of guanylate cyclase, which is coupled to specific ANF receptors on the RC cell.</jats:p> |
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| author | Ueno, M., Rondon, I., Beckman, B., Brookins, J., Nakashima, J., Cole, F. E., Fisher, J. W. |
| author_facet | Ueno, M., Rondon, I., Beckman, B., Brookins, J., Nakashima, J., Cole, F. E., Fisher, J. W., Ueno, M., Rondon, I., Beckman, B., Brookins, J., Nakashima, J., Cole, F. E., Fisher, J. W. |
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| description | <jats:p>The present studies were undertaken to assess the effects of atrial natriuretic factor (ANF) on erythropoietin (Ep) secretion in Ep-producing renal carcinoma (RC) cells using a sensitive radioimmunoassay for Ep. Human ANF produced a significant dose-related increase in Ep secretion at concentrations of 10(-7) and 10(-6) M when compared with vehicle controls. ANF (greater than or equal to 10(-9) M) also significantly increased the intracellular guanosine 3',5'-cyclic monophosphate (cGMP) concentration after 5-min incubation with the RC cells. Scatchard analysis of the human 125I-labeled ANF binding data indicated that the RC cells contain a single class of binding sites with a dissociation constant (Kd) of 93 +/- 1 pM and a binding capacity of 2,190 +/- 750 sites/cell. Incubation of the RC cells with 8-bromo-cGMP in concentrations of 10(-7)-10(-5) M also produced a significant dose-related enhancement of Ep secretion. These findings suggest that the increase in Ep secretion in response to ANF can be attributed, at least in part, to activation of guanylate cyclase, which is coupled to specific ANF receptors on the RC cell.</jats:p> |
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| imprint_str_mv | American Physiological Society, 1990 |
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| spelling | Ueno, M. Rondon, I. Beckman, B. Brookins, J. Nakashima, J. Cole, F. E. Fisher, J. W. 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.1990.259.3.c427 <jats:p>The present studies were undertaken to assess the effects of atrial natriuretic factor (ANF) on erythropoietin (Ep) secretion in Ep-producing renal carcinoma (RC) cells using a sensitive radioimmunoassay for Ep. Human ANF produced a significant dose-related increase in Ep secretion at concentrations of 10(-7) and 10(-6) M when compared with vehicle controls. ANF (greater than or equal to 10(-9) M) also significantly increased the intracellular guanosine 3',5'-cyclic monophosphate (cGMP) concentration after 5-min incubation with the RC cells. Scatchard analysis of the human 125I-labeled ANF binding data indicated that the RC cells contain a single class of binding sites with a dissociation constant (Kd) of 93 +/- 1 pM and a binding capacity of 2,190 +/- 750 sites/cell. Incubation of the RC cells with 8-bromo-cGMP in concentrations of 10(-7)-10(-5) M also produced a significant dose-related enhancement of Ep secretion. These findings suggest that the increase in Ep secretion in response to ANF can be attributed, at least in part, to activation of guanylate cyclase, which is coupled to specific ANF receptors on the RC cell.</jats:p> Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor American Journal of Physiology-Cell Physiology |
| spellingShingle | Ueno, M., Rondon, I., Beckman, B., Brookins, J., Nakashima, J., Cole, F. E., Fisher, J. W., American Journal of Physiology-Cell Physiology, Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor, Cell Biology, Physiology |
| title | Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| title_full | Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| title_fullStr | Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| title_full_unstemmed | Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| title_short | Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| title_sort | increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| title_unstemmed | Increased secretion of erythropoietin in human renal carcinoma cells in response to atrial natriuretic factor |
| topic | Cell Biology, Physiology |
| url | http://dx.doi.org/10.1152/ajpcell.1990.259.3.c427 |