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The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway
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| Zeitschriftentitel: | American Journal of Physiology-Cell Physiology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | American Journal of Physiology-Cell Physiology, 297, 2009, 4, S. C916-C927 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
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| Schlagwörter: |
| author_facet |
Kanika, Nirmala Devi Tar, Moses Tong, Yuehong Kuppam, Dwaraka Srinivasa Rao Melman, Arnold Davies, Kelvin Paul Kanika, Nirmala Devi Tar, Moses Tong, Yuehong Kuppam, Dwaraka Srinivasa Rao Melman, Arnold Davies, Kelvin Paul |
|---|---|
| author |
Kanika, Nirmala Devi Tar, Moses Tong, Yuehong Kuppam, Dwaraka Srinivasa Rao Melman, Arnold Davies, Kelvin Paul |
| spellingShingle |
Kanika, Nirmala Devi Tar, Moses Tong, Yuehong Kuppam, Dwaraka Srinivasa Rao Melman, Arnold Davies, Kelvin Paul American Journal of Physiology-Cell Physiology The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway Cell Biology Physiology |
| author_sort |
kanika, nirmala devi |
| spelling |
Kanika, Nirmala Devi Tar, Moses Tong, Yuehong Kuppam, Dwaraka Srinivasa Rao Melman, Arnold Davies, Kelvin Paul 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00656.2008 <jats:p> Intracorporal injection of plasmids encoding opiorphins into retired breeder rats can result in animals developing a priapic-like condition. Microarray analysis demonstrated that following intracorporal gene transfer of plasmids expressing opiorphins the most significantly upregulated gene in corporal tissue was the ornithine decarboxylase gene (ODC). Quantitative RT-PCR confirmed the upregulation of ODC, as well as other genes involved in polyamine synthesis, such as arginase-I and -II, polyamine oxidase, spermidine synthase, spermidine acetyltransferase (SAT), and S-adenosylmethionine decarboxylase. Western blot analysis demonstrated upregulation of arginase-I and -II, ODC, and SAT at the protein level. Levels of the polyamine putrescine were upregulated in animals treated with opiorphin-expressing plasmids compared with controls. A direct role for the upregulation of polyamine synthesis in the development of the priapic-like condition was supported by the observation that the ODC inhibitor 1,3-diaminopropane, when added to the drinking water of animals treated with plasmids expressing opiorphins, prevented experimental priapism. We also demonstrate that in sickle cell mice, another model of priapism, there is increased expression of the mouse opiorphin homologue in corporal tissue compared with the background strain at a life stage prior to evidence of priapism. At a life stage when there is onset of priapism, there is increased expression of the enzymes involved in polyamine synthesis (ODC and arginase-I and -II). Our results suggest that the upregulation of enzymes involved in the polyamine synthetic pathway may play a role in the development of experimental priapism and represent a target for the prevention of priapism. </jats:p> The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway American Journal of Physiology-Cell Physiology |
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| title |
The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| title_unstemmed |
The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| title_full |
The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| title_fullStr |
The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| title_full_unstemmed |
The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| title_short |
The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| title_sort |
the mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| topic |
Cell Biology Physiology |
| url |
http://dx.doi.org/10.1152/ajpcell.00656.2008 |
| publishDate |
2009 |
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C916-C927 |
| description |
<jats:p> Intracorporal injection of plasmids encoding opiorphins into retired breeder rats can result in animals developing a priapic-like condition. Microarray analysis demonstrated that following intracorporal gene transfer of plasmids expressing opiorphins the most significantly upregulated gene in corporal tissue was the ornithine decarboxylase gene (ODC). Quantitative RT-PCR confirmed the upregulation of ODC, as well as other genes involved in polyamine synthesis, such as arginase-I and -II, polyamine oxidase, spermidine synthase, spermidine acetyltransferase (SAT), and S-adenosylmethionine decarboxylase. Western blot analysis demonstrated upregulation of arginase-I and -II, ODC, and SAT at the protein level. Levels of the polyamine putrescine were upregulated in animals treated with opiorphin-expressing plasmids compared with controls. A direct role for the upregulation of polyamine synthesis in the development of the priapic-like condition was supported by the observation that the ODC inhibitor 1,3-diaminopropane, when added to the drinking water of animals treated with plasmids expressing opiorphins, prevented experimental priapism. We also demonstrate that in sickle cell mice, another model of priapism, there is increased expression of the mouse opiorphin homologue in corporal tissue compared with the background strain at a life stage prior to evidence of priapism. At a life stage when there is onset of priapism, there is increased expression of the enzymes involved in polyamine synthesis (ODC and arginase-I and -II). Our results suggest that the upregulation of enzymes involved in the polyamine synthetic pathway may play a role in the development of experimental priapism and represent a target for the prevention of priapism. </jats:p> |
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| author | Kanika, Nirmala Devi, Tar, Moses, Tong, Yuehong, Kuppam, Dwaraka Srinivasa Rao, Melman, Arnold, Davies, Kelvin Paul |
| author_facet | Kanika, Nirmala Devi, Tar, Moses, Tong, Yuehong, Kuppam, Dwaraka Srinivasa Rao, Melman, Arnold, Davies, Kelvin Paul, Kanika, Nirmala Devi, Tar, Moses, Tong, Yuehong, Kuppam, Dwaraka Srinivasa Rao, Melman, Arnold, Davies, Kelvin Paul |
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| description | <jats:p> Intracorporal injection of plasmids encoding opiorphins into retired breeder rats can result in animals developing a priapic-like condition. Microarray analysis demonstrated that following intracorporal gene transfer of plasmids expressing opiorphins the most significantly upregulated gene in corporal tissue was the ornithine decarboxylase gene (ODC). Quantitative RT-PCR confirmed the upregulation of ODC, as well as other genes involved in polyamine synthesis, such as arginase-I and -II, polyamine oxidase, spermidine synthase, spermidine acetyltransferase (SAT), and S-adenosylmethionine decarboxylase. Western blot analysis demonstrated upregulation of arginase-I and -II, ODC, and SAT at the protein level. Levels of the polyamine putrescine were upregulated in animals treated with opiorphin-expressing plasmids compared with controls. A direct role for the upregulation of polyamine synthesis in the development of the priapic-like condition was supported by the observation that the ODC inhibitor 1,3-diaminopropane, when added to the drinking water of animals treated with plasmids expressing opiorphins, prevented experimental priapism. We also demonstrate that in sickle cell mice, another model of priapism, there is increased expression of the mouse opiorphin homologue in corporal tissue compared with the background strain at a life stage prior to evidence of priapism. At a life stage when there is onset of priapism, there is increased expression of the enzymes involved in polyamine synthesis (ODC and arginase-I and -II). Our results suggest that the upregulation of enzymes involved in the polyamine synthetic pathway may play a role in the development of experimental priapism and represent a target for the prevention of priapism. </jats:p> |
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| institution | DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
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| spelling | Kanika, Nirmala Devi Tar, Moses Tong, Yuehong Kuppam, Dwaraka Srinivasa Rao Melman, Arnold Davies, Kelvin Paul 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00656.2008 <jats:p> Intracorporal injection of plasmids encoding opiorphins into retired breeder rats can result in animals developing a priapic-like condition. Microarray analysis demonstrated that following intracorporal gene transfer of plasmids expressing opiorphins the most significantly upregulated gene in corporal tissue was the ornithine decarboxylase gene (ODC). Quantitative RT-PCR confirmed the upregulation of ODC, as well as other genes involved in polyamine synthesis, such as arginase-I and -II, polyamine oxidase, spermidine synthase, spermidine acetyltransferase (SAT), and S-adenosylmethionine decarboxylase. Western blot analysis demonstrated upregulation of arginase-I and -II, ODC, and SAT at the protein level. Levels of the polyamine putrescine were upregulated in animals treated with opiorphin-expressing plasmids compared with controls. A direct role for the upregulation of polyamine synthesis in the development of the priapic-like condition was supported by the observation that the ODC inhibitor 1,3-diaminopropane, when added to the drinking water of animals treated with plasmids expressing opiorphins, prevented experimental priapism. We also demonstrate that in sickle cell mice, another model of priapism, there is increased expression of the mouse opiorphin homologue in corporal tissue compared with the background strain at a life stage prior to evidence of priapism. At a life stage when there is onset of priapism, there is increased expression of the enzymes involved in polyamine synthesis (ODC and arginase-I and -II). Our results suggest that the upregulation of enzymes involved in the polyamine synthetic pathway may play a role in the development of experimental priapism and represent a target for the prevention of priapism. </jats:p> The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway American Journal of Physiology-Cell Physiology |
| spellingShingle | Kanika, Nirmala Devi, Tar, Moses, Tong, Yuehong, Kuppam, Dwaraka Srinivasa Rao, Melman, Arnold, Davies, Kelvin Paul, American Journal of Physiology-Cell Physiology, The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway, Cell Biology, Physiology |
| title | The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| title_full | The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| title_fullStr | The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| title_full_unstemmed | The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| title_short | The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| title_sort | the mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| title_unstemmed | The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway |
| topic | Cell Biology, Physiology |
| url | http://dx.doi.org/10.1152/ajpcell.00656.2008 |