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Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3
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| Zeitschriftentitel: | American Journal of Physiology-Cell Physiology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | American Journal of Physiology-Cell Physiology, 282, 2002, 3, S. C487-C493 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
|
| Schlagwörter: |
| author_facet |
Xu, Hua Bai, Liqun Collins, James F. Ghishan, Fayez K. Xu, Hua Bai, Liqun Collins, James F. Ghishan, Fayez K. |
|---|---|
| author |
Xu, Hua Bai, Liqun Collins, James F. Ghishan, Fayez K. |
| spellingShingle |
Xu, Hua Bai, Liqun Collins, James F. Ghishan, Fayez K. American Journal of Physiology-Cell Physiology Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3 Cell Biology Physiology |
| author_sort |
xu, hua |
| spelling |
Xu, Hua Bai, Liqun Collins, James F. Ghishan, Fayez K. 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00412.2001 <jats:p>The current studies were designed to characterize type IIb sodium-inorganic phosphate (P<jats:sub>i</jats:sub>) cotransporter (NaP<jats:sub>i</jats:sub>-IIb) expression and to assess the effect of 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>on NaP<jats:sub>i</jats:sub>-IIb gene expression during rat ontogeny. Sodium-dependent P<jats:sub>i</jats:sub>absorption by intestinal brush-border membrane vesicles (BBMVs) decreased with age, and NaP<jats:sub>i</jats:sub>-IIb gene expression also decreased proportionally with age. 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>treatment increased intestinal BBMV P<jats:sub>i</jats:sub>absorption by ∼2.5-fold in suckling rats and by ∼2.1-fold in adult rats. 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>treatment also increased NaP<jats:sub>i</jats:sub>-IIb mRNA abundance by ∼2-fold in 14-day-old rats but had no effect on mRNA expression in adults. Furthermore, in rat intestinal epithelial (RIE) cells, 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>increased NaP<jats:sub>i</jats:sub>-IIb mRNA abundance, an effect that was abolished by actinomycin D. Additionally, human NaP<jats:sub>i</jats:sub>-IIb gene promoter activity in transiently transfected RIE cells showed ∼1.6-fold increase after 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>treatment. In conclusion, we demonstrate that the age-related decrease in intestinal sodium-dependent P<jats:sub>i</jats:sub>absorption correlates with decreased NaP<jats:sub>i</jats:sub>-IIb mRNA expression. Our data also suggest that the effect of 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>on NaP<jats:sub>i</jats:sub>-IIb expression is at least partially mediated by gene transcription in suckling rats.</jats:p> Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)<sub>2</sub>vitamin D<sub>3</sub> American Journal of Physiology-Cell Physiology |
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American Physiological Society, 2002 |
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American Physiological Society, 2002 |
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American Journal of Physiology-Cell Physiology |
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| title |
Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3 |
| title_unstemmed |
Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3 |
| title_full |
Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3 |
| title_fullStr |
Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3 |
| title_full_unstemmed |
Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3 |
| title_short |
Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3 |
| title_sort |
age-dependent regulation of rat intestinal type iib sodium-phosphate cotransporter by 1,25-(oh)<sub>2</sub>vitamin d<sub>3</sub> |
| topic |
Cell Biology Physiology |
| url |
http://dx.doi.org/10.1152/ajpcell.00412.2001 |
| publishDate |
2002 |
| physical |
C487-C493 |
| description |
<jats:p>The current studies were designed to characterize type IIb sodium-inorganic phosphate (P<jats:sub>i</jats:sub>) cotransporter (NaP<jats:sub>i</jats:sub>-IIb) expression and to assess the effect of 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>on NaP<jats:sub>i</jats:sub>-IIb gene expression during rat ontogeny. Sodium-dependent P<jats:sub>i</jats:sub>absorption by intestinal brush-border membrane vesicles (BBMVs) decreased with age, and NaP<jats:sub>i</jats:sub>-IIb gene expression also decreased proportionally with age. 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>treatment increased intestinal BBMV P<jats:sub>i</jats:sub>absorption by ∼2.5-fold in suckling rats and by ∼2.1-fold in adult rats. 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>treatment also increased NaP<jats:sub>i</jats:sub>-IIb mRNA abundance by ∼2-fold in 14-day-old rats but had no effect on mRNA expression in adults. Furthermore, in rat intestinal epithelial (RIE) cells, 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>increased NaP<jats:sub>i</jats:sub>-IIb mRNA abundance, an effect that was abolished by actinomycin D. Additionally, human NaP<jats:sub>i</jats:sub>-IIb gene promoter activity in transiently transfected RIE cells showed ∼1.6-fold increase after 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>treatment. In conclusion, we demonstrate that the age-related decrease in intestinal sodium-dependent P<jats:sub>i</jats:sub>absorption correlates with decreased NaP<jats:sub>i</jats:sub>-IIb mRNA expression. Our data also suggest that the effect of 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>on NaP<jats:sub>i</jats:sub>-IIb expression is at least partially mediated by gene transcription in suckling rats.</jats:p> |
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| author | Xu, Hua, Bai, Liqun, Collins, James F., Ghishan, Fayez K. |
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| description | <jats:p>The current studies were designed to characterize type IIb sodium-inorganic phosphate (P<jats:sub>i</jats:sub>) cotransporter (NaP<jats:sub>i</jats:sub>-IIb) expression and to assess the effect of 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>on NaP<jats:sub>i</jats:sub>-IIb gene expression during rat ontogeny. Sodium-dependent P<jats:sub>i</jats:sub>absorption by intestinal brush-border membrane vesicles (BBMVs) decreased with age, and NaP<jats:sub>i</jats:sub>-IIb gene expression also decreased proportionally with age. 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>treatment increased intestinal BBMV P<jats:sub>i</jats:sub>absorption by ∼2.5-fold in suckling rats and by ∼2.1-fold in adult rats. 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>treatment also increased NaP<jats:sub>i</jats:sub>-IIb mRNA abundance by ∼2-fold in 14-day-old rats but had no effect on mRNA expression in adults. Furthermore, in rat intestinal epithelial (RIE) cells, 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>increased NaP<jats:sub>i</jats:sub>-IIb mRNA abundance, an effect that was abolished by actinomycin D. Additionally, human NaP<jats:sub>i</jats:sub>-IIb gene promoter activity in transiently transfected RIE cells showed ∼1.6-fold increase after 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>treatment. In conclusion, we demonstrate that the age-related decrease in intestinal sodium-dependent P<jats:sub>i</jats:sub>absorption correlates with decreased NaP<jats:sub>i</jats:sub>-IIb mRNA expression. Our data also suggest that the effect of 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>on NaP<jats:sub>i</jats:sub>-IIb expression is at least partially mediated by gene transcription in suckling rats.</jats:p> |
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| imprint | American Physiological Society, 2002 |
| imprint_str_mv | American Physiological Society, 2002 |
| institution | DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275 |
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| physical | C487-C493 |
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| series | American Journal of Physiology-Cell Physiology |
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| spelling | Xu, Hua Bai, Liqun Collins, James F. Ghishan, Fayez K. 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00412.2001 <jats:p>The current studies were designed to characterize type IIb sodium-inorganic phosphate (P<jats:sub>i</jats:sub>) cotransporter (NaP<jats:sub>i</jats:sub>-IIb) expression and to assess the effect of 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>on NaP<jats:sub>i</jats:sub>-IIb gene expression during rat ontogeny. Sodium-dependent P<jats:sub>i</jats:sub>absorption by intestinal brush-border membrane vesicles (BBMVs) decreased with age, and NaP<jats:sub>i</jats:sub>-IIb gene expression also decreased proportionally with age. 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>treatment increased intestinal BBMV P<jats:sub>i</jats:sub>absorption by ∼2.5-fold in suckling rats and by ∼2.1-fold in adult rats. 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>treatment also increased NaP<jats:sub>i</jats:sub>-IIb mRNA abundance by ∼2-fold in 14-day-old rats but had no effect on mRNA expression in adults. Furthermore, in rat intestinal epithelial (RIE) cells, 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>increased NaP<jats:sub>i</jats:sub>-IIb mRNA abundance, an effect that was abolished by actinomycin D. Additionally, human NaP<jats:sub>i</jats:sub>-IIb gene promoter activity in transiently transfected RIE cells showed ∼1.6-fold increase after 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>treatment. In conclusion, we demonstrate that the age-related decrease in intestinal sodium-dependent P<jats:sub>i</jats:sub>absorption correlates with decreased NaP<jats:sub>i</jats:sub>-IIb mRNA expression. Our data also suggest that the effect of 1,25-(OH)<jats:sub>2</jats:sub>vitamin D<jats:sub>3</jats:sub>on NaP<jats:sub>i</jats:sub>-IIb expression is at least partially mediated by gene transcription in suckling rats.</jats:p> Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)<sub>2</sub>vitamin D<sub>3</sub> American Journal of Physiology-Cell Physiology |
| spellingShingle | Xu, Hua, Bai, Liqun, Collins, James F., Ghishan, Fayez K., American Journal of Physiology-Cell Physiology, Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3, Cell Biology, Physiology |
| title | Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3 |
| title_full | Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3 |
| title_fullStr | Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3 |
| title_full_unstemmed | Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3 |
| title_short | Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3 |
| title_sort | age-dependent regulation of rat intestinal type iib sodium-phosphate cotransporter by 1,25-(oh)<sub>2</sub>vitamin d<sub>3</sub> |
| title_unstemmed | Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2vitamin D3 |
| topic | Cell Biology, Physiology |
| url | http://dx.doi.org/10.1152/ajpcell.00412.2001 |