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Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells
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| Zeitschriftentitel: | American Journal of Physiology-Cell Physiology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | American Journal of Physiology-Cell Physiology, 290, 2006, 4, S. C1000-C1008 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
|
| Schlagwörter: |
| author_facet |
Kim, Moon Young Liang, Guo Hua Kim, Ji Aee Kim, Young Ju Oh, Seikwan Suh, Suk Hyo Kim, Moon Young Liang, Guo Hua Kim, Ji Aee Kim, Young Ju Oh, Seikwan Suh, Suk Hyo |
|---|---|
| author |
Kim, Moon Young Liang, Guo Hua Kim, Ji Aee Kim, Young Ju Oh, Seikwan Suh, Suk Hyo |
| spellingShingle |
Kim, Moon Young Liang, Guo Hua Kim, Ji Aee Kim, Young Ju Oh, Seikwan Suh, Suk Hyo American Journal of Physiology-Cell Physiology Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells Cell Biology Physiology |
| author_sort |
kim, moon young |
| spelling |
Kim, Moon Young Liang, Guo Hua Kim, Ji Aee Kim, Young Ju Oh, Seikwan Suh, Suk Hyo 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00353.2005 <jats:p>The effect of sphingosine-1-phosphate (S1P) on large-conductance Ca<jats:sup>2+</jats:sup>-activated K<jats:sup>+</jats:sup>(BK<jats:sub>Ca</jats:sub>) channels was examined in primary cultured human umbilical vein endothelial cells by measuring intracellular Ca<jats:sup>2+</jats:sup>concentration ([Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>), whole cell membrane currents, and single-channel activity. In nystatin-perforated current-clamped cells, S1P hyperpolarized the membrane and simultaneously increased [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>. [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>and membrane potentials were strongly correlated. In whole cell clamped cells, BK<jats:sub>Ca</jats:sub>currents were activated by increasing [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>via cell dialysis with pipette solution, and the activated BK<jats:sub>Ca</jats:sub>currents were further enhanced by S1P. When [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>was buffered at 1 μM, the S1P concentration required to evoke half-maximal activation was 403 ± 13 nM. In inside-out patches, when S1P was included in the bath solution, S1P enhanced BK<jats:sub>Ca</jats:sub>channel activity in a reversible manner and shifted the relationship between Ca<jats:sup>2+</jats:sup>concentration in the bath solution and the mean open probability to the left. In whole cell clamped cells or inside-out patches loaded with guanosine 5′- O-(2-thiodiphosphate) (GDPβS; 1 mM) using a patch pipette, GDPβS application or pretreatment of cells with pertussis toxin (100 ng/ml) for 15 h did not affect S1P-induced BK<jats:sub>Ca</jats:sub>current and channel activation. These results suggest that S1P enhances BK<jats:sub>Ca</jats:sub>channel activity by increasing Ca<jats:sup>2+</jats:sup>sensitivity. This channel activation hyperpolarizes the membrane and thereby increases Ca<jats:sup>2+</jats:sup>influx through Ca<jats:sup>2+</jats:sup>entry channels. Inasmuch as S1P activates BK<jats:sub>Ca</jats:sub>channels via a mechanism independent of G protein-coupled receptors, S1P may be a component of the intracellular second messenger that is involved in Ca<jats:sup>2+</jats:sup>mobilization in human endothelial cells.</jats:p> Sphingosine-1-phosphate activates BK<sub>Ca</sub>channels independently of G protein-coupled receptor in human endothelial cells American Journal of Physiology-Cell Physiology |
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10.1152/ajpcell.00353.2005 |
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Biologie |
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American Physiological Society, 2006 |
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American Physiological Society, 2006 |
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kim2006sphingosine1phosphateactivatesbkcachannelsindependentlyofgproteincoupledreceptorinhumanendothelialcells |
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2006 |
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American Physiological Society |
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American Journal of Physiology-Cell Physiology |
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49 |
| title |
Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells |
| title_unstemmed |
Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells |
| title_full |
Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells |
| title_fullStr |
Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells |
| title_full_unstemmed |
Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells |
| title_short |
Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells |
| title_sort |
sphingosine-1-phosphate activates bk<sub>ca</sub>channels independently of g protein-coupled receptor in human endothelial cells |
| topic |
Cell Biology Physiology |
| url |
http://dx.doi.org/10.1152/ajpcell.00353.2005 |
| publishDate |
2006 |
| physical |
C1000-C1008 |
| description |
<jats:p>The effect of sphingosine-1-phosphate (S1P) on large-conductance Ca<jats:sup>2+</jats:sup>-activated K<jats:sup>+</jats:sup>(BK<jats:sub>Ca</jats:sub>) channels was examined in primary cultured human umbilical vein endothelial cells by measuring intracellular Ca<jats:sup>2+</jats:sup>concentration ([Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>), whole cell membrane currents, and single-channel activity. In nystatin-perforated current-clamped cells, S1P hyperpolarized the membrane and simultaneously increased [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>. [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>and membrane potentials were strongly correlated. In whole cell clamped cells, BK<jats:sub>Ca</jats:sub>currents were activated by increasing [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>via cell dialysis with pipette solution, and the activated BK<jats:sub>Ca</jats:sub>currents were further enhanced by S1P. When [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>was buffered at 1 μM, the S1P concentration required to evoke half-maximal activation was 403 ± 13 nM. In inside-out patches, when S1P was included in the bath solution, S1P enhanced BK<jats:sub>Ca</jats:sub>channel activity in a reversible manner and shifted the relationship between Ca<jats:sup>2+</jats:sup>concentration in the bath solution and the mean open probability to the left. In whole cell clamped cells or inside-out patches loaded with guanosine 5′- O-(2-thiodiphosphate) (GDPβS; 1 mM) using a patch pipette, GDPβS application or pretreatment of cells with pertussis toxin (100 ng/ml) for 15 h did not affect S1P-induced BK<jats:sub>Ca</jats:sub>current and channel activation. These results suggest that S1P enhances BK<jats:sub>Ca</jats:sub>channel activity by increasing Ca<jats:sup>2+</jats:sup>sensitivity. This channel activation hyperpolarizes the membrane and thereby increases Ca<jats:sup>2+</jats:sup>influx through Ca<jats:sup>2+</jats:sup>entry channels. Inasmuch as S1P activates BK<jats:sub>Ca</jats:sub>channels via a mechanism independent of G protein-coupled receptors, S1P may be a component of the intracellular second messenger that is involved in Ca<jats:sup>2+</jats:sup>mobilization in human endothelial cells.</jats:p> |
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| author | Kim, Moon Young, Liang, Guo Hua, Kim, Ji Aee, Kim, Young Ju, Oh, Seikwan, Suh, Suk Hyo |
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| description | <jats:p>The effect of sphingosine-1-phosphate (S1P) on large-conductance Ca<jats:sup>2+</jats:sup>-activated K<jats:sup>+</jats:sup>(BK<jats:sub>Ca</jats:sub>) channels was examined in primary cultured human umbilical vein endothelial cells by measuring intracellular Ca<jats:sup>2+</jats:sup>concentration ([Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>), whole cell membrane currents, and single-channel activity. In nystatin-perforated current-clamped cells, S1P hyperpolarized the membrane and simultaneously increased [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>. [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>and membrane potentials were strongly correlated. In whole cell clamped cells, BK<jats:sub>Ca</jats:sub>currents were activated by increasing [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>via cell dialysis with pipette solution, and the activated BK<jats:sub>Ca</jats:sub>currents were further enhanced by S1P. When [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>was buffered at 1 μM, the S1P concentration required to evoke half-maximal activation was 403 ± 13 nM. In inside-out patches, when S1P was included in the bath solution, S1P enhanced BK<jats:sub>Ca</jats:sub>channel activity in a reversible manner and shifted the relationship between Ca<jats:sup>2+</jats:sup>concentration in the bath solution and the mean open probability to the left. In whole cell clamped cells or inside-out patches loaded with guanosine 5′- O-(2-thiodiphosphate) (GDPβS; 1 mM) using a patch pipette, GDPβS application or pretreatment of cells with pertussis toxin (100 ng/ml) for 15 h did not affect S1P-induced BK<jats:sub>Ca</jats:sub>current and channel activation. These results suggest that S1P enhances BK<jats:sub>Ca</jats:sub>channel activity by increasing Ca<jats:sup>2+</jats:sup>sensitivity. This channel activation hyperpolarizes the membrane and thereby increases Ca<jats:sup>2+</jats:sup>influx through Ca<jats:sup>2+</jats:sup>entry channels. Inasmuch as S1P activates BK<jats:sub>Ca</jats:sub>channels via a mechanism independent of G protein-coupled receptors, S1P may be a component of the intracellular second messenger that is involved in Ca<jats:sup>2+</jats:sup>mobilization in human endothelial cells.</jats:p> |
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| imprint_str_mv | American Physiological Society, 2006 |
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| spelling | Kim, Moon Young Liang, Guo Hua Kim, Ji Aee Kim, Young Ju Oh, Seikwan Suh, Suk Hyo 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00353.2005 <jats:p>The effect of sphingosine-1-phosphate (S1P) on large-conductance Ca<jats:sup>2+</jats:sup>-activated K<jats:sup>+</jats:sup>(BK<jats:sub>Ca</jats:sub>) channels was examined in primary cultured human umbilical vein endothelial cells by measuring intracellular Ca<jats:sup>2+</jats:sup>concentration ([Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>), whole cell membrane currents, and single-channel activity. In nystatin-perforated current-clamped cells, S1P hyperpolarized the membrane and simultaneously increased [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>. [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>and membrane potentials were strongly correlated. In whole cell clamped cells, BK<jats:sub>Ca</jats:sub>currents were activated by increasing [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>via cell dialysis with pipette solution, and the activated BK<jats:sub>Ca</jats:sub>currents were further enhanced by S1P. When [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>was buffered at 1 μM, the S1P concentration required to evoke half-maximal activation was 403 ± 13 nM. In inside-out patches, when S1P was included in the bath solution, S1P enhanced BK<jats:sub>Ca</jats:sub>channel activity in a reversible manner and shifted the relationship between Ca<jats:sup>2+</jats:sup>concentration in the bath solution and the mean open probability to the left. In whole cell clamped cells or inside-out patches loaded with guanosine 5′- O-(2-thiodiphosphate) (GDPβS; 1 mM) using a patch pipette, GDPβS application or pretreatment of cells with pertussis toxin (100 ng/ml) for 15 h did not affect S1P-induced BK<jats:sub>Ca</jats:sub>current and channel activation. These results suggest that S1P enhances BK<jats:sub>Ca</jats:sub>channel activity by increasing Ca<jats:sup>2+</jats:sup>sensitivity. This channel activation hyperpolarizes the membrane and thereby increases Ca<jats:sup>2+</jats:sup>influx through Ca<jats:sup>2+</jats:sup>entry channels. Inasmuch as S1P activates BK<jats:sub>Ca</jats:sub>channels via a mechanism independent of G protein-coupled receptors, S1P may be a component of the intracellular second messenger that is involved in Ca<jats:sup>2+</jats:sup>mobilization in human endothelial cells.</jats:p> Sphingosine-1-phosphate activates BK<sub>Ca</sub>channels independently of G protein-coupled receptor in human endothelial cells American Journal of Physiology-Cell Physiology |
| spellingShingle | Kim, Moon Young, Liang, Guo Hua, Kim, Ji Aee, Kim, Young Ju, Oh, Seikwan, Suh, Suk Hyo, American Journal of Physiology-Cell Physiology, Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells, Cell Biology, Physiology |
| title | Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells |
| title_full | Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells |
| title_fullStr | Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells |
| title_full_unstemmed | Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells |
| title_short | Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells |
| title_sort | sphingosine-1-phosphate activates bk<sub>ca</sub>channels independently of g protein-coupled receptor in human endothelial cells |
| title_unstemmed | Sphingosine-1-phosphate activates BKCachannels independently of G protein-coupled receptor in human endothelial cells |
| topic | Cell Biology, Physiology |
| url | http://dx.doi.org/10.1152/ajpcell.00353.2005 |