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The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent
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| Zeitschriftentitel: | American Journal of Physiology-Cell Physiology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | American Journal of Physiology-Cell Physiology, 290, 2006, 4, S. C1041-C1050 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
|
| Schlagwörter: |
| author_facet |
Debold, E. P. Romatowski, J. Fitts, R. H. Debold, E. P. Romatowski, J. Fitts, R. H. |
|---|---|
| author |
Debold, E. P. Romatowski, J. Fitts, R. H. |
| spellingShingle |
Debold, E. P. Romatowski, J. Fitts, R. H. American Journal of Physiology-Cell Physiology The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent Cell Biology Physiology |
| author_sort |
debold, e. p. |
| spelling |
Debold, E. P. Romatowski, J. Fitts, R. H. 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00342.2005 <jats:p>Increases in P<jats:sub>i</jats:sub>combined with decreases in myoplasmic Ca<jats:sup>2+</jats:sup>are believed to cause a significant portion of the decrease in muscular force during fatigue. To investigate this further, we determined the effect of 30 mM P<jats:sub>i</jats:sub>on the force-Ca<jats:sup>2+</jats:sup>relationship of chemically skinned single muscle fibers at near-physiological temperature (30°C). Fibers isolated from rat soleus (slow) and gastrocnemius (fast) muscle were subjected to a series of solutions with an increasing free Ca<jats:sup>2+</jats:sup>concentration in the presence and absence of 30 mM P<jats:sub>i</jats:sub>at both low (15°C) and high (30°C) temperature. In slow fibers, 30 mM P<jats:sub>i</jats:sub>significantly increased the Ca<jats:sup>2+</jats:sup>required to elicit measurable force, referred to as the activation threshold at both low and high temperatures; however, the effect was twofold greater at the higher temperature. In fast fibers, the activation threshold was unaffected by elevating P<jats:sub>i</jats:sub>at 15°C but was significantly increased at 30°C. At both low and high temperatures, 30 mM P<jats:sub>i</jats:sub>increased the Ca<jats:sup>2+</jats:sup>required to elicit half-maximal force (pCa<jats:sub>50</jats:sub>) in both slow and fast fibers, with the effect of P<jats:sub>i</jats:sub>twofold greater at the higher temperature. These data suggest that during fatigue, reductions in the myoplasmic Ca<jats:sup>2+</jats:sup>and increases in P<jats:sub>i</jats:sub>act synergistically to reduce muscular force. Consequently, the combined changes in these ions likely account for a greater portion of fatigue than previously predicted based on studies at lower temperatures or high temperatures at saturating Ca<jats:sup>2+</jats:sup>levels.</jats:p> The depressive effect of P<sub>i</sub>on the force-pCa relationship in skinned single muscle fibers is temperature dependent American Journal of Physiology-Cell Physiology |
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| title |
The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent |
| title_unstemmed |
The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent |
| title_full |
The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent |
| title_fullStr |
The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent |
| title_full_unstemmed |
The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent |
| title_short |
The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent |
| title_sort |
the depressive effect of p<sub>i</sub>on the force-pca relationship in skinned single muscle fibers is temperature dependent |
| topic |
Cell Biology Physiology |
| url |
http://dx.doi.org/10.1152/ajpcell.00342.2005 |
| publishDate |
2006 |
| physical |
C1041-C1050 |
| description |
<jats:p>Increases in P<jats:sub>i</jats:sub>combined with decreases in myoplasmic Ca<jats:sup>2+</jats:sup>are believed to cause a significant portion of the decrease in muscular force during fatigue. To investigate this further, we determined the effect of 30 mM P<jats:sub>i</jats:sub>on the force-Ca<jats:sup>2+</jats:sup>relationship of chemically skinned single muscle fibers at near-physiological temperature (30°C). Fibers isolated from rat soleus (slow) and gastrocnemius (fast) muscle were subjected to a series of solutions with an increasing free Ca<jats:sup>2+</jats:sup>concentration in the presence and absence of 30 mM P<jats:sub>i</jats:sub>at both low (15°C) and high (30°C) temperature. In slow fibers, 30 mM P<jats:sub>i</jats:sub>significantly increased the Ca<jats:sup>2+</jats:sup>required to elicit measurable force, referred to as the activation threshold at both low and high temperatures; however, the effect was twofold greater at the higher temperature. In fast fibers, the activation threshold was unaffected by elevating P<jats:sub>i</jats:sub>at 15°C but was significantly increased at 30°C. At both low and high temperatures, 30 mM P<jats:sub>i</jats:sub>increased the Ca<jats:sup>2+</jats:sup>required to elicit half-maximal force (pCa<jats:sub>50</jats:sub>) in both slow and fast fibers, with the effect of P<jats:sub>i</jats:sub>twofold greater at the higher temperature. These data suggest that during fatigue, reductions in the myoplasmic Ca<jats:sup>2+</jats:sup>and increases in P<jats:sub>i</jats:sub>act synergistically to reduce muscular force. Consequently, the combined changes in these ions likely account for a greater portion of fatigue than previously predicted based on studies at lower temperatures or high temperatures at saturating Ca<jats:sup>2+</jats:sup>levels.</jats:p> |
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| author | Debold, E. P., Romatowski, J., Fitts, R. H. |
| author_facet | Debold, E. P., Romatowski, J., Fitts, R. H., Debold, E. P., Romatowski, J., Fitts, R. H. |
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| description | <jats:p>Increases in P<jats:sub>i</jats:sub>combined with decreases in myoplasmic Ca<jats:sup>2+</jats:sup>are believed to cause a significant portion of the decrease in muscular force during fatigue. To investigate this further, we determined the effect of 30 mM P<jats:sub>i</jats:sub>on the force-Ca<jats:sup>2+</jats:sup>relationship of chemically skinned single muscle fibers at near-physiological temperature (30°C). Fibers isolated from rat soleus (slow) and gastrocnemius (fast) muscle were subjected to a series of solutions with an increasing free Ca<jats:sup>2+</jats:sup>concentration in the presence and absence of 30 mM P<jats:sub>i</jats:sub>at both low (15°C) and high (30°C) temperature. In slow fibers, 30 mM P<jats:sub>i</jats:sub>significantly increased the Ca<jats:sup>2+</jats:sup>required to elicit measurable force, referred to as the activation threshold at both low and high temperatures; however, the effect was twofold greater at the higher temperature. In fast fibers, the activation threshold was unaffected by elevating P<jats:sub>i</jats:sub>at 15°C but was significantly increased at 30°C. At both low and high temperatures, 30 mM P<jats:sub>i</jats:sub>increased the Ca<jats:sup>2+</jats:sup>required to elicit half-maximal force (pCa<jats:sub>50</jats:sub>) in both slow and fast fibers, with the effect of P<jats:sub>i</jats:sub>twofold greater at the higher temperature. These data suggest that during fatigue, reductions in the myoplasmic Ca<jats:sup>2+</jats:sup>and increases in P<jats:sub>i</jats:sub>act synergistically to reduce muscular force. Consequently, the combined changes in these ions likely account for a greater portion of fatigue than previously predicted based on studies at lower temperatures or high temperatures at saturating Ca<jats:sup>2+</jats:sup>levels.</jats:p> |
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| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14 |
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| physical | C1041-C1050 |
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| series | American Journal of Physiology-Cell Physiology |
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| spelling | Debold, E. P. Romatowski, J. Fitts, R. H. 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00342.2005 <jats:p>Increases in P<jats:sub>i</jats:sub>combined with decreases in myoplasmic Ca<jats:sup>2+</jats:sup>are believed to cause a significant portion of the decrease in muscular force during fatigue. To investigate this further, we determined the effect of 30 mM P<jats:sub>i</jats:sub>on the force-Ca<jats:sup>2+</jats:sup>relationship of chemically skinned single muscle fibers at near-physiological temperature (30°C). Fibers isolated from rat soleus (slow) and gastrocnemius (fast) muscle were subjected to a series of solutions with an increasing free Ca<jats:sup>2+</jats:sup>concentration in the presence and absence of 30 mM P<jats:sub>i</jats:sub>at both low (15°C) and high (30°C) temperature. In slow fibers, 30 mM P<jats:sub>i</jats:sub>significantly increased the Ca<jats:sup>2+</jats:sup>required to elicit measurable force, referred to as the activation threshold at both low and high temperatures; however, the effect was twofold greater at the higher temperature. In fast fibers, the activation threshold was unaffected by elevating P<jats:sub>i</jats:sub>at 15°C but was significantly increased at 30°C. At both low and high temperatures, 30 mM P<jats:sub>i</jats:sub>increased the Ca<jats:sup>2+</jats:sup>required to elicit half-maximal force (pCa<jats:sub>50</jats:sub>) in both slow and fast fibers, with the effect of P<jats:sub>i</jats:sub>twofold greater at the higher temperature. These data suggest that during fatigue, reductions in the myoplasmic Ca<jats:sup>2+</jats:sup>and increases in P<jats:sub>i</jats:sub>act synergistically to reduce muscular force. Consequently, the combined changes in these ions likely account for a greater portion of fatigue than previously predicted based on studies at lower temperatures or high temperatures at saturating Ca<jats:sup>2+</jats:sup>levels.</jats:p> The depressive effect of P<sub>i</sub>on the force-pCa relationship in skinned single muscle fibers is temperature dependent American Journal of Physiology-Cell Physiology |
| spellingShingle | Debold, E. P., Romatowski, J., Fitts, R. H., American Journal of Physiology-Cell Physiology, The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent, Cell Biology, Physiology |
| title | The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent |
| title_full | The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent |
| title_fullStr | The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent |
| title_full_unstemmed | The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent |
| title_short | The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent |
| title_sort | the depressive effect of p<sub>i</sub>on the force-pca relationship in skinned single muscle fibers is temperature dependent |
| title_unstemmed | The depressive effect of Pion the force-pCa relationship in skinned single muscle fibers is temperature dependent |
| topic | Cell Biology, Physiology |
| url | http://dx.doi.org/10.1152/ajpcell.00342.2005 |