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Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts
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| Zeitschriftentitel: | American Journal of Physiology-Cell Physiology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | American Journal of Physiology-Cell Physiology, 285, 2003, 1, S. C22-C30 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
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| Schlagwörter: |
| author_facet |
Bräuer, Margot Frei, Eva Claes, Lutz Grissmer, Stephan Jäger, Heike Bräuer, Margot Frei, Eva Claes, Lutz Grissmer, Stephan Jäger, Heike |
|---|---|
| author |
Bräuer, Margot Frei, Eva Claes, Lutz Grissmer, Stephan Jäger, Heike |
| spellingShingle |
Bräuer, Margot Frei, Eva Claes, Lutz Grissmer, Stephan Jäger, Heike American Journal of Physiology-Cell Physiology Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts Cell Biology Physiology |
| author_sort |
bräuer, margot |
| spelling |
Bräuer, Margot Frei, Eva Claes, Lutz Grissmer, Stephan Jäger, Heike 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00289.2002 <jats:p>The whole cell recording mode of the patch-clamp technique was used to study the effect of hypotonic NaCl or isotonic high-KCl solution on membrane currents in a human osteoblast-like cell line, C1. Both hypotonic NaCl or isotonic high-KCl solution activated Cl<jats:sup>–</jats:sup>channels expressed in these cells as described previously. The reversal potential of the induced Cl<jats:sup>–</jats:sup>current is more negative when activated through hypotonic NaCl solution (–47 ± 5 mV; n = 6) compared with activation through isotonic high-KCl solution (–35 ± 3 mV; n = 8). This difference can be explained by an increase in intracellular [Cl<jats:sup>–</jats:sup>] through the activity of a K-Cl cotransporter. Potassium aspartate was unable to activate the current, and furosemide or DIOA suppressed the increase in Cl<jats:sup>–</jats:sup>current induced by isotonic high-KCl solution. In addition, we used the polymerase chain reaction to demonstrate the presence of KCC1–KCC4 mRNA in the osteoblast-like cell line. From these results, we conclude that human osteoblasts express functional K-Cl cotransporters in their cell membrane that seem to be able to induce the indirect activation of volume-sensitive Cl<jats:sup>–</jats:sup>channels by KCl through an increase in the intracellular ion concentration followed by water influx and cell swelling.</jats:p> Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl<sup>–</sup>channels in human osteoblasts American Journal of Physiology-Cell Physiology |
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10.1152/ajpcell.00289.2002 |
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American Journal of Physiology-Cell Physiology |
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| title |
Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts |
| title_unstemmed |
Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts |
| title_full |
Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts |
| title_fullStr |
Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts |
| title_full_unstemmed |
Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts |
| title_short |
Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts |
| title_sort |
influence of k-cl cotransporter activity on activation of volume-sensitive cl<sup>–</sup>channels in human osteoblasts |
| topic |
Cell Biology Physiology |
| url |
http://dx.doi.org/10.1152/ajpcell.00289.2002 |
| publishDate |
2003 |
| physical |
C22-C30 |
| description |
<jats:p>The whole cell recording mode of the patch-clamp technique was used to study the effect of hypotonic NaCl or isotonic high-KCl solution on membrane currents in a human osteoblast-like cell line, C1. Both hypotonic NaCl or isotonic high-KCl solution activated Cl<jats:sup>–</jats:sup>channels expressed in these cells as described previously. The reversal potential of the induced Cl<jats:sup>–</jats:sup>current is more negative when activated through hypotonic NaCl solution (–47 ± 5 mV; n = 6) compared with activation through isotonic high-KCl solution (–35 ± 3 mV; n = 8). This difference can be explained by an increase in intracellular [Cl<jats:sup>–</jats:sup>] through the activity of a K-Cl cotransporter. Potassium aspartate was unable to activate the current, and furosemide or DIOA suppressed the increase in Cl<jats:sup>–</jats:sup>current induced by isotonic high-KCl solution. In addition, we used the polymerase chain reaction to demonstrate the presence of KCC1–KCC4 mRNA in the osteoblast-like cell line. From these results, we conclude that human osteoblasts express functional K-Cl cotransporters in their cell membrane that seem to be able to induce the indirect activation of volume-sensitive Cl<jats:sup>–</jats:sup>channels by KCl through an increase in the intracellular ion concentration followed by water influx and cell swelling.</jats:p> |
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| author | Bräuer, Margot, Frei, Eva, Claes, Lutz, Grissmer, Stephan, Jäger, Heike |
| author_facet | Bräuer, Margot, Frei, Eva, Claes, Lutz, Grissmer, Stephan, Jäger, Heike, Bräuer, Margot, Frei, Eva, Claes, Lutz, Grissmer, Stephan, Jäger, Heike |
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| description | <jats:p>The whole cell recording mode of the patch-clamp technique was used to study the effect of hypotonic NaCl or isotonic high-KCl solution on membrane currents in a human osteoblast-like cell line, C1. Both hypotonic NaCl or isotonic high-KCl solution activated Cl<jats:sup>–</jats:sup>channels expressed in these cells as described previously. The reversal potential of the induced Cl<jats:sup>–</jats:sup>current is more negative when activated through hypotonic NaCl solution (–47 ± 5 mV; n = 6) compared with activation through isotonic high-KCl solution (–35 ± 3 mV; n = 8). This difference can be explained by an increase in intracellular [Cl<jats:sup>–</jats:sup>] through the activity of a K-Cl cotransporter. Potassium aspartate was unable to activate the current, and furosemide or DIOA suppressed the increase in Cl<jats:sup>–</jats:sup>current induced by isotonic high-KCl solution. In addition, we used the polymerase chain reaction to demonstrate the presence of KCC1–KCC4 mRNA in the osteoblast-like cell line. From these results, we conclude that human osteoblasts express functional K-Cl cotransporters in their cell membrane that seem to be able to induce the indirect activation of volume-sensitive Cl<jats:sup>–</jats:sup>channels by KCl through an increase in the intracellular ion concentration followed by water influx and cell swelling.</jats:p> |
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| spelling | Bräuer, Margot Frei, Eva Claes, Lutz Grissmer, Stephan Jäger, Heike 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00289.2002 <jats:p>The whole cell recording mode of the patch-clamp technique was used to study the effect of hypotonic NaCl or isotonic high-KCl solution on membrane currents in a human osteoblast-like cell line, C1. Both hypotonic NaCl or isotonic high-KCl solution activated Cl<jats:sup>–</jats:sup>channels expressed in these cells as described previously. The reversal potential of the induced Cl<jats:sup>–</jats:sup>current is more negative when activated through hypotonic NaCl solution (–47 ± 5 mV; n = 6) compared with activation through isotonic high-KCl solution (–35 ± 3 mV; n = 8). This difference can be explained by an increase in intracellular [Cl<jats:sup>–</jats:sup>] through the activity of a K-Cl cotransporter. Potassium aspartate was unable to activate the current, and furosemide or DIOA suppressed the increase in Cl<jats:sup>–</jats:sup>current induced by isotonic high-KCl solution. In addition, we used the polymerase chain reaction to demonstrate the presence of KCC1–KCC4 mRNA in the osteoblast-like cell line. From these results, we conclude that human osteoblasts express functional K-Cl cotransporters in their cell membrane that seem to be able to induce the indirect activation of volume-sensitive Cl<jats:sup>–</jats:sup>channels by KCl through an increase in the intracellular ion concentration followed by water influx and cell swelling.</jats:p> Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl<sup>–</sup>channels in human osteoblasts American Journal of Physiology-Cell Physiology |
| spellingShingle | Bräuer, Margot, Frei, Eva, Claes, Lutz, Grissmer, Stephan, Jäger, Heike, American Journal of Physiology-Cell Physiology, Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts, Cell Biology, Physiology |
| title | Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts |
| title_full | Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts |
| title_fullStr | Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts |
| title_full_unstemmed | Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts |
| title_short | Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts |
| title_sort | influence of k-cl cotransporter activity on activation of volume-sensitive cl<sup>–</sup>channels in human osteoblasts |
| title_unstemmed | Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl–channels in human osteoblasts |
| topic | Cell Biology, Physiology |
| url | http://dx.doi.org/10.1152/ajpcell.00289.2002 |