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Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells
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| Zeitschriftentitel: | American Journal of Reproductive Immunology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | American Journal of Reproductive Immunology, 46, 2001, 3, S. 226-231 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
FRIEBE‐HOFFMANN, ULRIKE CHIAO, JYE‐PING RAUK, PHILLIP N. FRIEBE‐HOFFMANN, ULRIKE CHIAO, JYE‐PING RAUK, PHILLIP N. |
|---|---|
| author |
FRIEBE‐HOFFMANN, ULRIKE CHIAO, JYE‐PING RAUK, PHILLIP N. |
| spellingShingle |
FRIEBE‐HOFFMANN, ULRIKE CHIAO, JYE‐PING RAUK, PHILLIP N. American Journal of Reproductive Immunology Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells Obstetrics and Gynecology Reproductive Medicine Immunology Immunology and Allergy Obstetrics and Gynecology Immunology |
| author_sort |
friebe‐hoffmann, ulrike |
| spelling |
FRIEBE‐HOFFMANN, ULRIKE CHIAO, JYE‐PING RAUK, PHILLIP N. 1046-7408 1600-0897 Wiley Obstetrics and Gynecology Reproductive Medicine Immunology Immunology and Allergy Obstetrics and Gynecology Immunology http://dx.doi.org/10.1034/j.1600-0897.2001.d01-6.x <jats:p>PROBLEM: Infection‐mediated preterm labor results in the production of inflammatory cytokines, including interleukin‐1β (IL‐1β) and interleukin‐6 (IL‐6). Oxytocin (OT) plays a key role in the process of labor. This study investigates the effect of IL‐1β and IL‐6 on intra‐and extracellular OT in human smooth muscle cells and evaluates IL‐1β induced changes in IL‐6 production. METHOD OF STUDY: Primary cultures of human myometrium, obtained from term pregnant women (<jats:italic>n</jats:italic>=7) were incubated with either IL‐1β or IL‐6 for 0–24 hr. Intra‐ and extracellular OT peptide concentrations were measured by radioimmunoassay and IL‐6 mRNA and protein were evaluated by reverse transcription polymerase chain reaction and enzyme‐linked immunosorbent assay. RESULTS: Both, IL‐1β and IL‐6 led to an increase in OT secretion, which was accompanied by a reduction of the intracellular OT peptide pool. IL‐1β significantly induced IL‐6 mRNA expression and protein secretion, which did not further enhance IL‐1β induced OT secretion. CONCLUSIONS: The induction of OT secretion by proinflammatory cytokines in human myometrium <jats:italic>in vitro</jats:italic>, supports the concept of a thus regulated infection‐triggered preterm labor process <jats:italic>in vivo</jats:italic>.</jats:p> Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells American Journal of Reproductive Immunology |
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DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Zi4 DE-Gla1 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 |
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Wiley, 2001 |
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Wiley, 2001 |
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1046-7408 1600-0897 |
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2001 |
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Wiley |
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American Journal of Reproductive Immunology |
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49 |
| title |
Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells |
| title_unstemmed |
Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells |
| title_full |
Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells |
| title_fullStr |
Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells |
| title_full_unstemmed |
Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells |
| title_short |
Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells |
| title_sort |
effect of il‐1β and il‐6 on oxytocin secretion in human uterine smooth muscle cells |
| topic |
Obstetrics and Gynecology Reproductive Medicine Immunology Immunology and Allergy Obstetrics and Gynecology Immunology |
| url |
http://dx.doi.org/10.1034/j.1600-0897.2001.d01-6.x |
| publishDate |
2001 |
| physical |
226-231 |
| description |
<jats:p>PROBLEM: Infection‐mediated preterm labor results in the production of inflammatory cytokines, including interleukin‐1β (IL‐1β) and interleukin‐6 (IL‐6). Oxytocin (OT) plays a key role in the process of labor. This study investigates the effect of IL‐1β and IL‐6 on intra‐and extracellular OT in human smooth muscle cells and evaluates IL‐1β induced changes in IL‐6 production.
METHOD OF STUDY: Primary cultures of human myometrium, obtained from term pregnant women (<jats:italic>n</jats:italic>=7) were incubated with either IL‐1β or IL‐6 for 0–24 hr. Intra‐ and extracellular OT peptide concentrations were measured by radioimmunoassay and IL‐6 mRNA and protein were evaluated by reverse transcription polymerase chain reaction and enzyme‐linked immunosorbent assay.
RESULTS: Both, IL‐1β and IL‐6 led to an increase in OT secretion, which was accompanied by a reduction of the intracellular OT peptide pool. IL‐1β significantly induced IL‐6 mRNA expression and protein secretion, which did not further enhance IL‐1β induced OT secretion.
CONCLUSIONS: The induction of OT secretion by proinflammatory cytokines in human myometrium <jats:italic>in vitro</jats:italic>, supports the concept of a thus regulated infection‐triggered preterm labor process <jats:italic>in vivo</jats:italic>.</jats:p> |
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| author | FRIEBE‐HOFFMANN, ULRIKE, CHIAO, JYE‐PING, RAUK, PHILLIP N. |
| author_facet | FRIEBE‐HOFFMANN, ULRIKE, CHIAO, JYE‐PING, RAUK, PHILLIP N., FRIEBE‐HOFFMANN, ULRIKE, CHIAO, JYE‐PING, RAUK, PHILLIP N. |
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| container_issue | 3 |
| container_start_page | 226 |
| container_title | American Journal of Reproductive Immunology |
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| description | <jats:p>PROBLEM: Infection‐mediated preterm labor results in the production of inflammatory cytokines, including interleukin‐1β (IL‐1β) and interleukin‐6 (IL‐6). Oxytocin (OT) plays a key role in the process of labor. This study investigates the effect of IL‐1β and IL‐6 on intra‐and extracellular OT in human smooth muscle cells and evaluates IL‐1β induced changes in IL‐6 production. METHOD OF STUDY: Primary cultures of human myometrium, obtained from term pregnant women (<jats:italic>n</jats:italic>=7) were incubated with either IL‐1β or IL‐6 for 0–24 hr. Intra‐ and extracellular OT peptide concentrations were measured by radioimmunoassay and IL‐6 mRNA and protein were evaluated by reverse transcription polymerase chain reaction and enzyme‐linked immunosorbent assay. RESULTS: Both, IL‐1β and IL‐6 led to an increase in OT secretion, which was accompanied by a reduction of the intracellular OT peptide pool. IL‐1β significantly induced IL‐6 mRNA expression and protein secretion, which did not further enhance IL‐1β induced OT secretion. CONCLUSIONS: The induction of OT secretion by proinflammatory cytokines in human myometrium <jats:italic>in vitro</jats:italic>, supports the concept of a thus regulated infection‐triggered preterm labor process <jats:italic>in vivo</jats:italic>.</jats:p> |
| doi_str_mv | 10.1034/j.1600-0897.2001.d01-6.x |
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| imprint | Wiley, 2001 |
| imprint_str_mv | Wiley, 2001 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229 |
| issn | 1046-7408, 1600-0897 |
| issn_str_mv | 1046-7408, 1600-0897 |
| language | English |
| last_indexed | 2024-03-01T15:53:18.428Z |
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| source_id | 49 |
| spelling | FRIEBE‐HOFFMANN, ULRIKE CHIAO, JYE‐PING RAUK, PHILLIP N. 1046-7408 1600-0897 Wiley Obstetrics and Gynecology Reproductive Medicine Immunology Immunology and Allergy Obstetrics and Gynecology Immunology http://dx.doi.org/10.1034/j.1600-0897.2001.d01-6.x <jats:p>PROBLEM: Infection‐mediated preterm labor results in the production of inflammatory cytokines, including interleukin‐1β (IL‐1β) and interleukin‐6 (IL‐6). Oxytocin (OT) plays a key role in the process of labor. This study investigates the effect of IL‐1β and IL‐6 on intra‐and extracellular OT in human smooth muscle cells and evaluates IL‐1β induced changes in IL‐6 production. METHOD OF STUDY: Primary cultures of human myometrium, obtained from term pregnant women (<jats:italic>n</jats:italic>=7) were incubated with either IL‐1β or IL‐6 for 0–24 hr. Intra‐ and extracellular OT peptide concentrations were measured by radioimmunoassay and IL‐6 mRNA and protein were evaluated by reverse transcription polymerase chain reaction and enzyme‐linked immunosorbent assay. RESULTS: Both, IL‐1β and IL‐6 led to an increase in OT secretion, which was accompanied by a reduction of the intracellular OT peptide pool. IL‐1β significantly induced IL‐6 mRNA expression and protein secretion, which did not further enhance IL‐1β induced OT secretion. CONCLUSIONS: The induction of OT secretion by proinflammatory cytokines in human myometrium <jats:italic>in vitro</jats:italic>, supports the concept of a thus regulated infection‐triggered preterm labor process <jats:italic>in vivo</jats:italic>.</jats:p> Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells American Journal of Reproductive Immunology |
| spellingShingle | FRIEBE‐HOFFMANN, ULRIKE, CHIAO, JYE‐PING, RAUK, PHILLIP N., American Journal of Reproductive Immunology, Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells, Obstetrics and Gynecology, Reproductive Medicine, Immunology, Immunology and Allergy, Obstetrics and Gynecology, Immunology |
| title | Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells |
| title_full | Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells |
| title_fullStr | Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells |
| title_full_unstemmed | Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells |
| title_short | Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells |
| title_sort | effect of il‐1β and il‐6 on oxytocin secretion in human uterine smooth muscle cells |
| title_unstemmed | Effect of IL‐1β and IL‐6 on Oxytocin Secretion in Human Uterine Smooth Muscle Cells |
| topic | Obstetrics and Gynecology, Reproductive Medicine, Immunology, Immunology and Allergy, Obstetrics and Gynecology, Immunology |
| url | http://dx.doi.org/10.1034/j.1600-0897.2001.d01-6.x |