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A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France
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| Zeitschriftentitel: | Epidemiology and Infection |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Epidemiology and Infection, 147, 2019 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cambridge University Press (CUP)
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| Schlagwörter: |
| author_facet |
Menu, E. Mary, C. Toga, I. Raoult, D. Ranque, S. Bittar, F. Menu, E. Mary, C. Toga, I. Raoult, D. Ranque, S. Bittar, F. |
|---|---|
| author |
Menu, E. Mary, C. Toga, I. Raoult, D. Ranque, S. Bittar, F. |
| spellingShingle |
Menu, E. Mary, C. Toga, I. Raoult, D. Ranque, S. Bittar, F. Epidemiology and Infection A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France Infectious Diseases Epidemiology |
| author_sort |
menu, e. |
| spelling |
Menu, E. Mary, C. Toga, I. Raoult, D. Ranque, S. Bittar, F. 0950-2688 1469-4409 Cambridge University Press (CUP) Infectious Diseases Epidemiology http://dx.doi.org/10.1017/s0950268819000165 <jats:title>Abstract</jats:title><jats:p>There is a scarcity of recent epidemiological data on intestinal parasitic infections in France. We conducted a prospective study aimed at estimating the prevalence of 10 enteric parasites in Marseille, France, using real-time polymerase chain reaction (PCR)-based diagnosis. A total of 643 faeces from 488 patients referred to the Parasitology-Mycology Laboratory of the University Hospital of Marseille over a 6 months period were included. DNA was extracted using a semi-automated method. Parasites of interest were detected using singleplex quantitative PCRs (qPCRs). For positive samples, the<jats:italic>Blastocystis</jats:italic>subtype was determined by sequence analysis. During the study, the overall prevalence of enteric parasites was 17%.<jats:italic>Blastocystis sp.</jats:italic>was the most frequent species (10.5%), followed by<jats:italic>Dientamoeba fragilis</jats:italic>(2.3%) and<jats:italic>Giardia intestinalis</jats:italic>(2.3%). The prevalence of other parasites was <1% each. The ST3<jats:italic>Blastocystis</jats:italic>subtype was predominant (43.6%) and the other subtypes identified were ST1, ST2, ST4 and ST6. This is the first time that a qPCR-based diagnosis has been used to survey the prevalence of 10 enteric parasites in a French University Hospital. This study confirms that fast, specific, sensitive and simultaneous detection in a single stool sample by qPCR clearly outperforms conventional microscopy-based diagnosis. Furthermore, qPCR is particularly well suited to surveying gastroenteritis agents.</jats:p> A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France Epidemiology and Infection |
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A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France |
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A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France |
| title_full |
A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France |
| title_fullStr |
A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France |
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A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France |
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A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France |
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a hospital qpcr-based survey of 10 gastrointestinal parasites in routine diagnostic screening, marseille, france |
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Infectious Diseases Epidemiology |
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http://dx.doi.org/10.1017/s0950268819000165 |
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2019 |
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<jats:title>Abstract</jats:title><jats:p>There is a scarcity of recent epidemiological data on intestinal parasitic infections in France. We conducted a prospective study aimed at estimating the prevalence of 10 enteric parasites in Marseille, France, using real-time polymerase chain reaction (PCR)-based diagnosis. A total of 643 faeces from 488 patients referred to the Parasitology-Mycology Laboratory of the University Hospital of Marseille over a 6 months period were included. DNA was extracted using a semi-automated method. Parasites of interest were detected using singleplex quantitative PCRs (qPCRs). For positive samples, the<jats:italic>Blastocystis</jats:italic>subtype was determined by sequence analysis. During the study, the overall prevalence of enteric parasites was 17%.<jats:italic>Blastocystis sp.</jats:italic>was the most frequent species (10.5%), followed by<jats:italic>Dientamoeba fragilis</jats:italic>(2.3%) and<jats:italic>Giardia intestinalis</jats:italic>(2.3%). The prevalence of other parasites was <1% each. The ST3<jats:italic>Blastocystis</jats:italic>subtype was predominant (43.6%) and the other subtypes identified were ST1, ST2, ST4 and ST6. This is the first time that a qPCR-based diagnosis has been used to survey the prevalence of 10 enteric parasites in a French University Hospital. This study confirms that fast, specific, sensitive and simultaneous detection in a single stool sample by qPCR clearly outperforms conventional microscopy-based diagnosis. Furthermore, qPCR is particularly well suited to surveying gastroenteritis agents.</jats:p> |
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| author | Menu, E., Mary, C., Toga, I., Raoult, D., Ranque, S., Bittar, F. |
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| description | <jats:title>Abstract</jats:title><jats:p>There is a scarcity of recent epidemiological data on intestinal parasitic infections in France. We conducted a prospective study aimed at estimating the prevalence of 10 enteric parasites in Marseille, France, using real-time polymerase chain reaction (PCR)-based diagnosis. A total of 643 faeces from 488 patients referred to the Parasitology-Mycology Laboratory of the University Hospital of Marseille over a 6 months period were included. DNA was extracted using a semi-automated method. Parasites of interest were detected using singleplex quantitative PCRs (qPCRs). For positive samples, the<jats:italic>Blastocystis</jats:italic>subtype was determined by sequence analysis. During the study, the overall prevalence of enteric parasites was 17%.<jats:italic>Blastocystis sp.</jats:italic>was the most frequent species (10.5%), followed by<jats:italic>Dientamoeba fragilis</jats:italic>(2.3%) and<jats:italic>Giardia intestinalis</jats:italic>(2.3%). The prevalence of other parasites was <1% each. The ST3<jats:italic>Blastocystis</jats:italic>subtype was predominant (43.6%) and the other subtypes identified were ST1, ST2, ST4 and ST6. This is the first time that a qPCR-based diagnosis has been used to survey the prevalence of 10 enteric parasites in a French University Hospital. This study confirms that fast, specific, sensitive and simultaneous detection in a single stool sample by qPCR clearly outperforms conventional microscopy-based diagnosis. Furthermore, qPCR is particularly well suited to surveying gastroenteritis agents.</jats:p> |
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| spelling | Menu, E. Mary, C. Toga, I. Raoult, D. Ranque, S. Bittar, F. 0950-2688 1469-4409 Cambridge University Press (CUP) Infectious Diseases Epidemiology http://dx.doi.org/10.1017/s0950268819000165 <jats:title>Abstract</jats:title><jats:p>There is a scarcity of recent epidemiological data on intestinal parasitic infections in France. We conducted a prospective study aimed at estimating the prevalence of 10 enteric parasites in Marseille, France, using real-time polymerase chain reaction (PCR)-based diagnosis. A total of 643 faeces from 488 patients referred to the Parasitology-Mycology Laboratory of the University Hospital of Marseille over a 6 months period were included. DNA was extracted using a semi-automated method. Parasites of interest were detected using singleplex quantitative PCRs (qPCRs). For positive samples, the<jats:italic>Blastocystis</jats:italic>subtype was determined by sequence analysis. During the study, the overall prevalence of enteric parasites was 17%.<jats:italic>Blastocystis sp.</jats:italic>was the most frequent species (10.5%), followed by<jats:italic>Dientamoeba fragilis</jats:italic>(2.3%) and<jats:italic>Giardia intestinalis</jats:italic>(2.3%). The prevalence of other parasites was <1% each. The ST3<jats:italic>Blastocystis</jats:italic>subtype was predominant (43.6%) and the other subtypes identified were ST1, ST2, ST4 and ST6. This is the first time that a qPCR-based diagnosis has been used to survey the prevalence of 10 enteric parasites in a French University Hospital. This study confirms that fast, specific, sensitive and simultaneous detection in a single stool sample by qPCR clearly outperforms conventional microscopy-based diagnosis. Furthermore, qPCR is particularly well suited to surveying gastroenteritis agents.</jats:p> A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France Epidemiology and Infection |
| spellingShingle | Menu, E., Mary, C., Toga, I., Raoult, D., Ranque, S., Bittar, F., Epidemiology and Infection, A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France, Infectious Diseases, Epidemiology |
| title | A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France |
| title_full | A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France |
| title_fullStr | A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France |
| title_full_unstemmed | A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France |
| title_short | A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France |
| title_sort | a hospital qpcr-based survey of 10 gastrointestinal parasites in routine diagnostic screening, marseille, france |
| title_unstemmed | A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France |
| topic | Infectious Diseases, Epidemiology |
| url | http://dx.doi.org/10.1017/s0950268819000165 |