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Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference
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| Zeitschriftentitel: | Bulletin of Entomological Research |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Bulletin of Entomological Research, 108, 2018, 3, S. 388-399 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cambridge University Press (CUP)
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| Schlagwörter: |
| author_facet |
Chen, Q.W. Jin, S. Zhang, L. Shen, Q.D. Wei, P. Wei, Z.M. Wang, S.G. Tang, B Chen, Q.W. Jin, S. Zhang, L. Shen, Q.D. Wei, P. Wei, Z.M. Wang, S.G. Tang, B |
|---|---|
| author |
Chen, Q.W. Jin, S. Zhang, L. Shen, Q.D. Wei, P. Wei, Z.M. Wang, S.G. Tang, B |
| spellingShingle |
Chen, Q.W. Jin, S. Zhang, L. Shen, Q.D. Wei, P. Wei, Z.M. Wang, S.G. Tang, B Bulletin of Entomological Research Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference Insect Science Agronomy and Crop Science General Medicine |
| author_sort |
chen, q.w. |
| spelling |
Chen, Q.W. Jin, S. Zhang, L. Shen, Q.D. Wei, P. Wei, Z.M. Wang, S.G. Tang, B 0007-4853 1475-2670 Cambridge University Press (CUP) Insect Science Agronomy and Crop Science General Medicine http://dx.doi.org/10.1017/s000748531700089x <jats:title>Abstract</jats:title><jats:p>RNA interference (RNAi) is a very effective technique for studying gene function and may be an efficient method for controlling pests. Trehalose-6-phosphate synthase (<jats:italic>TPS</jats:italic>), which plays a key role in the synthesis of trehalose and insect development, was cloned in <jats:italic>Tribolium castaneum</jats:italic> (Herbst) (<jats:italic>TcTPS</jats:italic>) and the putative functions were studied using RNAi via the injection of double-stranded RNA (dsRNA) corresponding to conserved TPS and trehalose-6-phosphate phosphatase domains. Expression analyses show that <jats:italic>TcTPS</jats:italic> is expressed higher in the fat body, while quantitative real-time polymerase chain reaction results show that the expression of four trehalase isoforms was significantly suppressed by dsTPS injection. Additionally, the expression of six chitin synthesis-related genes, such as hexokinase 2 and glutamine-fructose-6-phosphate aminotransferase, was suppressed at 48 and 72 h post-dsTPS-1 and dsTPS-2 RNA injection, which were two dsTPS fragments that had been designed for two different locations in TcTPS open reading frame, and that trehalose content and trehalase 1 activity decreased significantly at 72 h post-dsRNA injection. Furthermore, <jats:italic>T. castaneum</jats:italic> injected with dsTPS-1 and dsTPS-2 RNA displayed significantly lower levels of chitin and could not complete the molting process from larvae to pupae, revealing abnormal molting phenotypes. These results demonstrate that silencing <jats:italic>TPS</jats:italic> gene leads to molting deformities and high mortality rates via regulation of gene expression in the chitin biosynthetic pathway, and may be a promising approach for pest control in the future.</jats:p> Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in <i>Tribolium castaneum</i> (Coleoptera: Tenebrionidae) revealed by RNA interference Bulletin of Entomological Research |
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| title |
Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference |
| title_unstemmed |
Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference |
| title_full |
Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference |
| title_fullStr |
Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference |
| title_full_unstemmed |
Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference |
| title_short |
Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference |
| title_sort |
regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in <i>tribolium castaneum</i> (coleoptera: tenebrionidae) revealed by rna interference |
| topic |
Insect Science Agronomy and Crop Science General Medicine |
| url |
http://dx.doi.org/10.1017/s000748531700089x |
| publishDate |
2018 |
| physical |
388-399 |
| description |
<jats:title>Abstract</jats:title><jats:p>RNA interference (RNAi) is a very effective technique for studying gene function and may be an efficient method for controlling pests. Trehalose-6-phosphate synthase (<jats:italic>TPS</jats:italic>), which plays a key role in the synthesis of trehalose and insect development, was cloned in <jats:italic>Tribolium castaneum</jats:italic> (Herbst) (<jats:italic>TcTPS</jats:italic>) and the putative functions were studied using RNAi via the injection of double-stranded RNA (dsRNA) corresponding to conserved TPS and trehalose-6-phosphate phosphatase domains. Expression analyses show that <jats:italic>TcTPS</jats:italic> is expressed higher in the fat body, while quantitative real-time polymerase chain reaction results show that the expression of four trehalase isoforms was significantly suppressed by dsTPS injection. Additionally, the expression of six chitin synthesis-related genes, such as hexokinase 2 and glutamine-fructose-6-phosphate aminotransferase, was suppressed at 48 and 72 h post-dsTPS-1 and dsTPS-2 RNA injection, which were two dsTPS fragments that had been designed for two different locations in TcTPS open reading frame, and that trehalose content and trehalase 1 activity decreased significantly at 72 h post-dsRNA injection. Furthermore, <jats:italic>T. castaneum</jats:italic> injected with dsTPS-1 and dsTPS-2 RNA displayed significantly lower levels of chitin and could not complete the molting process from larvae to pupae, revealing abnormal molting phenotypes. These results demonstrate that silencing <jats:italic>TPS</jats:italic> gene leads to molting deformities and high mortality rates via regulation of gene expression in the chitin biosynthetic pathway, and may be a promising approach for pest control in the future.</jats:p> |
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| author | Chen, Q.W., Jin, S., Zhang, L., Shen, Q.D., Wei, P., Wei, Z.M., Wang, S.G., Tang, B |
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| description | <jats:title>Abstract</jats:title><jats:p>RNA interference (RNAi) is a very effective technique for studying gene function and may be an efficient method for controlling pests. Trehalose-6-phosphate synthase (<jats:italic>TPS</jats:italic>), which plays a key role in the synthesis of trehalose and insect development, was cloned in <jats:italic>Tribolium castaneum</jats:italic> (Herbst) (<jats:italic>TcTPS</jats:italic>) and the putative functions were studied using RNAi via the injection of double-stranded RNA (dsRNA) corresponding to conserved TPS and trehalose-6-phosphate phosphatase domains. Expression analyses show that <jats:italic>TcTPS</jats:italic> is expressed higher in the fat body, while quantitative real-time polymerase chain reaction results show that the expression of four trehalase isoforms was significantly suppressed by dsTPS injection. Additionally, the expression of six chitin synthesis-related genes, such as hexokinase 2 and glutamine-fructose-6-phosphate aminotransferase, was suppressed at 48 and 72 h post-dsTPS-1 and dsTPS-2 RNA injection, which were two dsTPS fragments that had been designed for two different locations in TcTPS open reading frame, and that trehalose content and trehalase 1 activity decreased significantly at 72 h post-dsRNA injection. Furthermore, <jats:italic>T. castaneum</jats:italic> injected with dsTPS-1 and dsTPS-2 RNA displayed significantly lower levels of chitin and could not complete the molting process from larvae to pupae, revealing abnormal molting phenotypes. These results demonstrate that silencing <jats:italic>TPS</jats:italic> gene leads to molting deformities and high mortality rates via regulation of gene expression in the chitin biosynthetic pathway, and may be a promising approach for pest control in the future.</jats:p> |
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| spelling | Chen, Q.W. Jin, S. Zhang, L. Shen, Q.D. Wei, P. Wei, Z.M. Wang, S.G. Tang, B 0007-4853 1475-2670 Cambridge University Press (CUP) Insect Science Agronomy and Crop Science General Medicine http://dx.doi.org/10.1017/s000748531700089x <jats:title>Abstract</jats:title><jats:p>RNA interference (RNAi) is a very effective technique for studying gene function and may be an efficient method for controlling pests. Trehalose-6-phosphate synthase (<jats:italic>TPS</jats:italic>), which plays a key role in the synthesis of trehalose and insect development, was cloned in <jats:italic>Tribolium castaneum</jats:italic> (Herbst) (<jats:italic>TcTPS</jats:italic>) and the putative functions were studied using RNAi via the injection of double-stranded RNA (dsRNA) corresponding to conserved TPS and trehalose-6-phosphate phosphatase domains. Expression analyses show that <jats:italic>TcTPS</jats:italic> is expressed higher in the fat body, while quantitative real-time polymerase chain reaction results show that the expression of four trehalase isoforms was significantly suppressed by dsTPS injection. Additionally, the expression of six chitin synthesis-related genes, such as hexokinase 2 and glutamine-fructose-6-phosphate aminotransferase, was suppressed at 48 and 72 h post-dsTPS-1 and dsTPS-2 RNA injection, which were two dsTPS fragments that had been designed for two different locations in TcTPS open reading frame, and that trehalose content and trehalase 1 activity decreased significantly at 72 h post-dsRNA injection. Furthermore, <jats:italic>T. castaneum</jats:italic> injected with dsTPS-1 and dsTPS-2 RNA displayed significantly lower levels of chitin and could not complete the molting process from larvae to pupae, revealing abnormal molting phenotypes. These results demonstrate that silencing <jats:italic>TPS</jats:italic> gene leads to molting deformities and high mortality rates via regulation of gene expression in the chitin biosynthetic pathway, and may be a promising approach for pest control in the future.</jats:p> Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in <i>Tribolium castaneum</i> (Coleoptera: Tenebrionidae) revealed by RNA interference Bulletin of Entomological Research |
| spellingShingle | Chen, Q.W., Jin, S., Zhang, L., Shen, Q.D., Wei, P., Wei, Z.M., Wang, S.G., Tang, B, Bulletin of Entomological Research, Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference, Insect Science, Agronomy and Crop Science, General Medicine |
| title | Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference |
| title_full | Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference |
| title_fullStr | Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference |
| title_full_unstemmed | Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference |
| title_short | Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference |
| title_sort | regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in <i>tribolium castaneum</i> (coleoptera: tenebrionidae) revealed by rna interference |
| title_unstemmed | Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference |
| topic | Insect Science, Agronomy and Crop Science, General Medicine |
| url | http://dx.doi.org/10.1017/s000748531700089x |