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Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver
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| Zeitschriftentitel: | British Journal of Nutrition |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | British Journal of Nutrition, 104, 2010, 7, S. 935-940 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cambridge University Press (CUP)
|
| Schlagwörter: |
| author_facet |
Fischer, Alexandra Gaedicke, Sonja Frank, Jan Döring, Frank Rimbach, Gerald Fischer, Alexandra Gaedicke, Sonja Frank, Jan Döring, Frank Rimbach, Gerald |
|---|---|
| author |
Fischer, Alexandra Gaedicke, Sonja Frank, Jan Döring, Frank Rimbach, Gerald |
| spellingShingle |
Fischer, Alexandra Gaedicke, Sonja Frank, Jan Döring, Frank Rimbach, Gerald British Journal of Nutrition Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver Nutrition and Dietetics Medicine (miscellaneous) |
| author_sort |
fischer, alexandra |
| spelling |
Fischer, Alexandra Gaedicke, Sonja Frank, Jan Döring, Frank Rimbach, Gerald 0007-1145 1475-2662 Cambridge University Press (CUP) Nutrition and Dietetics Medicine (miscellaneous) http://dx.doi.org/10.1017/s0007114510001649 <jats:p>The aim of the present study was to determine the effects of a 6-month dietary vitamin E (VE) deficiency on DNA methylation and gene expression in rat liver. Two enzymes, 5-α-steroid reductase type 1 (SRD5A1) and the regulatory subunit of γ-glutamylcysteinyl synthetase (GCLM), which are differentially expressed on the mRNA level, were analysed for promoter methylation in putative cytosine-phospho-guanine (CpG) island regions located at the 5′ end using base-specific cleavage and matrix-assisted laser desorption ionisation time-of-flight MS. A twofold increase in the mRNA level of SRD5A1 gene and a twofold decrease in the mRNA level of GCLM gene in VE-deficient animals were not associated with different CpG methylation of the analysed promoter region. Furthermore, global DNA methylation was not significantly different in these two groups. Thus, the present results indicate that the VE-induced regulation of SRD5A1 and GCLM in rat liver is not directly mediated by changes in promoter DNA methylation.</jats:p> Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver British Journal of Nutrition |
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| title |
Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver |
| title_unstemmed |
Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver |
| title_full |
Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver |
| title_fullStr |
Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver |
| title_full_unstemmed |
Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver |
| title_short |
Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver |
| title_sort |
dietary vitamin e deficiency does not affect global and specific dna methylation patterns in rat liver |
| topic |
Nutrition and Dietetics Medicine (miscellaneous) |
| url |
http://dx.doi.org/10.1017/s0007114510001649 |
| publishDate |
2010 |
| physical |
935-940 |
| description |
<jats:p>The aim of the present study was to determine the effects of a 6-month dietary vitamin E (VE) deficiency on DNA methylation and gene expression in rat liver. Two enzymes, 5-α-steroid reductase type 1 (SRD5A1) and the regulatory subunit of γ-glutamylcysteinyl synthetase (GCLM), which are differentially expressed on the mRNA level, were analysed for promoter methylation in putative cytosine-phospho-guanine (CpG) island regions located at the 5′ end using base-specific cleavage and matrix-assisted laser desorption ionisation time-of-flight MS. A twofold increase in the mRNA level of SRD5A1 gene and a twofold decrease in the mRNA level of GCLM gene in VE-deficient animals were not associated with different CpG methylation of the analysed promoter region. Furthermore, global DNA methylation was not significantly different in these two groups. Thus, the present results indicate that the VE-induced regulation of SRD5A1 and GCLM in rat liver is not directly mediated by changes in promoter DNA methylation.</jats:p> |
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| author | Fischer, Alexandra, Gaedicke, Sonja, Frank, Jan, Döring, Frank, Rimbach, Gerald |
| author_facet | Fischer, Alexandra, Gaedicke, Sonja, Frank, Jan, Döring, Frank, Rimbach, Gerald, Fischer, Alexandra, Gaedicke, Sonja, Frank, Jan, Döring, Frank, Rimbach, Gerald |
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| description | <jats:p>The aim of the present study was to determine the effects of a 6-month dietary vitamin E (VE) deficiency on DNA methylation and gene expression in rat liver. Two enzymes, 5-α-steroid reductase type 1 (SRD5A1) and the regulatory subunit of γ-glutamylcysteinyl synthetase (GCLM), which are differentially expressed on the mRNA level, were analysed for promoter methylation in putative cytosine-phospho-guanine (CpG) island regions located at the 5′ end using base-specific cleavage and matrix-assisted laser desorption ionisation time-of-flight MS. A twofold increase in the mRNA level of SRD5A1 gene and a twofold decrease in the mRNA level of GCLM gene in VE-deficient animals were not associated with different CpG methylation of the analysed promoter region. Furthermore, global DNA methylation was not significantly different in these two groups. Thus, the present results indicate that the VE-induced regulation of SRD5A1 and GCLM in rat liver is not directly mediated by changes in promoter DNA methylation.</jats:p> |
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| spelling | Fischer, Alexandra Gaedicke, Sonja Frank, Jan Döring, Frank Rimbach, Gerald 0007-1145 1475-2662 Cambridge University Press (CUP) Nutrition and Dietetics Medicine (miscellaneous) http://dx.doi.org/10.1017/s0007114510001649 <jats:p>The aim of the present study was to determine the effects of a 6-month dietary vitamin E (VE) deficiency on DNA methylation and gene expression in rat liver. Two enzymes, 5-α-steroid reductase type 1 (SRD5A1) and the regulatory subunit of γ-glutamylcysteinyl synthetase (GCLM), which are differentially expressed on the mRNA level, were analysed for promoter methylation in putative cytosine-phospho-guanine (CpG) island regions located at the 5′ end using base-specific cleavage and matrix-assisted laser desorption ionisation time-of-flight MS. A twofold increase in the mRNA level of SRD5A1 gene and a twofold decrease in the mRNA level of GCLM gene in VE-deficient animals were not associated with different CpG methylation of the analysed promoter region. Furthermore, global DNA methylation was not significantly different in these two groups. Thus, the present results indicate that the VE-induced regulation of SRD5A1 and GCLM in rat liver is not directly mediated by changes in promoter DNA methylation.</jats:p> Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver British Journal of Nutrition |
| spellingShingle | Fischer, Alexandra, Gaedicke, Sonja, Frank, Jan, Döring, Frank, Rimbach, Gerald, British Journal of Nutrition, Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver, Nutrition and Dietetics, Medicine (miscellaneous) |
| title | Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver |
| title_full | Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver |
| title_fullStr | Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver |
| title_full_unstemmed | Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver |
| title_short | Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver |
| title_sort | dietary vitamin e deficiency does not affect global and specific dna methylation patterns in rat liver |
| title_unstemmed | Dietary vitamin E deficiency does not affect global and specific DNA methylation patterns in rat liver |
| topic | Nutrition and Dietetics, Medicine (miscellaneous) |
| url | http://dx.doi.org/10.1017/s0007114510001649 |