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Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo
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| Zeitschriftentitel: | British Journal of Nutrition |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | British Journal of Nutrition, 104, 2010, 3, S. 336-345 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cambridge University Press (CUP)
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| Schlagwörter: |
| author_facet |
Boomgaarden, Inka Egert, Sarah Rimbach, Gerald Wolffram, Siegfried Müller, Manfred J. Döring, Frank Boomgaarden, Inka Egert, Sarah Rimbach, Gerald Wolffram, Siegfried Müller, Manfred J. Döring, Frank |
|---|---|
| author |
Boomgaarden, Inka Egert, Sarah Rimbach, Gerald Wolffram, Siegfried Müller, Manfred J. Döring, Frank |
| spellingShingle |
Boomgaarden, Inka Egert, Sarah Rimbach, Gerald Wolffram, Siegfried Müller, Manfred J. Döring, Frank British Journal of Nutrition Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo Nutrition and Dietetics Medicine (miscellaneous) |
| author_sort |
boomgaarden, inka |
| spelling |
Boomgaarden, Inka Egert, Sarah Rimbach, Gerald Wolffram, Siegfried Müller, Manfred J. Döring, Frank 0007-1145 1475-2662 Cambridge University Press (CUP) Nutrition and Dietetics Medicine (miscellaneous) http://dx.doi.org/10.1017/s0007114510000711 <jats:p>Quercetin has been described as having a wide range of beneficial effects in humans, ranging from anti-carcinogenic properties to reducing the risk of CVD. Nevertheless, underlying molecular mechanisms have been mostly investigated <jats:italic>in vitro</jats:italic>. Here, we tested whether a daily supplementation of quercetin leads to reproducible changes in human monocyte gene expression profiles. In study I, quercetin in varying dosages was given to healthy subjects for 2 weeks. RNA from monocytes isolated at the beginning and end of the study from subjects receiving 150 mg quercetin per d was subjected to transcriptome-wide microarray analysis. In study II, a double-blind cross-over study, twenty subjects exhibiting a ‘cardiovascular risk phenotype’ received 150 mg quercetin or placebo daily for 6 weeks each and served as the verification group. Microarray analysis revealed a number of differentially expressed genes. The most significantly represented functional groups were those of the immune system, nucleic acid metabolism, apoptosis and <jats:italic>O</jats:italic>-glycan biosynthesis. Twenty-four genes were chosen for technical replication and independent verification by quantitative real-time PCR. When comparing placebo and quercetin treatment, four genes showed significantly different expression changes (<jats:italic>C1GALT1</jats:italic>, <jats:italic>O</jats:italic>-glycan biosynthesis; <jats:italic>GM2A</jats:italic>, glycolipid catabolism; <jats:italic>HDGF</jats:italic>, cell proliferation; <jats:italic>SERPINB9</jats:italic>, apoptosis). However, these were minimal in respect to magnitude of fold change. In conclusion, although microarray analysis revealed extensive effects of quercetin on gene expression, the employment of a placebo-controlled study design showed no comparable results for twenty-four verification targets. This emphasises the need for stringent designs in nutritional intervention studies with the aim to identify relevant changes in gene expression.</jats:p> Quercetin supplementation and its effect on human monocyte gene expression profiles <i>in vivo</i> British Journal of Nutrition |
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| title |
Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo |
| title_unstemmed |
Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo |
| title_full |
Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo |
| title_fullStr |
Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo |
| title_full_unstemmed |
Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo |
| title_short |
Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo |
| title_sort |
quercetin supplementation and its effect on human monocyte gene expression profiles <i>in vivo</i> |
| topic |
Nutrition and Dietetics Medicine (miscellaneous) |
| url |
http://dx.doi.org/10.1017/s0007114510000711 |
| publishDate |
2010 |
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336-345 |
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<jats:p>Quercetin has been described as having a wide range of beneficial effects in humans, ranging from anti-carcinogenic properties to reducing the risk of CVD. Nevertheless, underlying molecular mechanisms have been mostly investigated <jats:italic>in vitro</jats:italic>. Here, we tested whether a daily supplementation of quercetin leads to reproducible changes in human monocyte gene expression profiles. In study I, quercetin in varying dosages was given to healthy subjects for 2 weeks. RNA from monocytes isolated at the beginning and end of the study from subjects receiving 150 mg quercetin per d was subjected to transcriptome-wide microarray analysis. In study II, a double-blind cross-over study, twenty subjects exhibiting a ‘cardiovascular risk phenotype’ received 150 mg quercetin or placebo daily for 6 weeks each and served as the verification group. Microarray analysis revealed a number of differentially expressed genes. The most significantly represented functional groups were those of the immune system, nucleic acid metabolism, apoptosis and <jats:italic>O</jats:italic>-glycan biosynthesis. Twenty-four genes were chosen for technical replication and independent verification by quantitative real-time PCR. When comparing placebo and quercetin treatment, four genes showed significantly different expression changes (<jats:italic>C1GALT1</jats:italic>, <jats:italic>O</jats:italic>-glycan biosynthesis; <jats:italic>GM2A</jats:italic>, glycolipid catabolism; <jats:italic>HDGF</jats:italic>, cell proliferation; <jats:italic>SERPINB9</jats:italic>, apoptosis). However, these were minimal in respect to magnitude of fold change. In conclusion, although microarray analysis revealed extensive effects of quercetin on gene expression, the employment of a placebo-controlled study design showed no comparable results for twenty-four verification targets. This emphasises the need for stringent designs in nutritional intervention studies with the aim to identify relevant changes in gene expression.</jats:p> |
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| author | Boomgaarden, Inka, Egert, Sarah, Rimbach, Gerald, Wolffram, Siegfried, Müller, Manfred J., Döring, Frank |
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| description | <jats:p>Quercetin has been described as having a wide range of beneficial effects in humans, ranging from anti-carcinogenic properties to reducing the risk of CVD. Nevertheless, underlying molecular mechanisms have been mostly investigated <jats:italic>in vitro</jats:italic>. Here, we tested whether a daily supplementation of quercetin leads to reproducible changes in human monocyte gene expression profiles. In study I, quercetin in varying dosages was given to healthy subjects for 2 weeks. RNA from monocytes isolated at the beginning and end of the study from subjects receiving 150 mg quercetin per d was subjected to transcriptome-wide microarray analysis. In study II, a double-blind cross-over study, twenty subjects exhibiting a ‘cardiovascular risk phenotype’ received 150 mg quercetin or placebo daily for 6 weeks each and served as the verification group. Microarray analysis revealed a number of differentially expressed genes. The most significantly represented functional groups were those of the immune system, nucleic acid metabolism, apoptosis and <jats:italic>O</jats:italic>-glycan biosynthesis. Twenty-four genes were chosen for technical replication and independent verification by quantitative real-time PCR. When comparing placebo and quercetin treatment, four genes showed significantly different expression changes (<jats:italic>C1GALT1</jats:italic>, <jats:italic>O</jats:italic>-glycan biosynthesis; <jats:italic>GM2A</jats:italic>, glycolipid catabolism; <jats:italic>HDGF</jats:italic>, cell proliferation; <jats:italic>SERPINB9</jats:italic>, apoptosis). However, these were minimal in respect to magnitude of fold change. In conclusion, although microarray analysis revealed extensive effects of quercetin on gene expression, the employment of a placebo-controlled study design showed no comparable results for twenty-four verification targets. This emphasises the need for stringent designs in nutritional intervention studies with the aim to identify relevant changes in gene expression.</jats:p> |
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| spelling | Boomgaarden, Inka Egert, Sarah Rimbach, Gerald Wolffram, Siegfried Müller, Manfred J. Döring, Frank 0007-1145 1475-2662 Cambridge University Press (CUP) Nutrition and Dietetics Medicine (miscellaneous) http://dx.doi.org/10.1017/s0007114510000711 <jats:p>Quercetin has been described as having a wide range of beneficial effects in humans, ranging from anti-carcinogenic properties to reducing the risk of CVD. Nevertheless, underlying molecular mechanisms have been mostly investigated <jats:italic>in vitro</jats:italic>. Here, we tested whether a daily supplementation of quercetin leads to reproducible changes in human monocyte gene expression profiles. In study I, quercetin in varying dosages was given to healthy subjects for 2 weeks. RNA from monocytes isolated at the beginning and end of the study from subjects receiving 150 mg quercetin per d was subjected to transcriptome-wide microarray analysis. In study II, a double-blind cross-over study, twenty subjects exhibiting a ‘cardiovascular risk phenotype’ received 150 mg quercetin or placebo daily for 6 weeks each and served as the verification group. Microarray analysis revealed a number of differentially expressed genes. The most significantly represented functional groups were those of the immune system, nucleic acid metabolism, apoptosis and <jats:italic>O</jats:italic>-glycan biosynthesis. Twenty-four genes were chosen for technical replication and independent verification by quantitative real-time PCR. When comparing placebo and quercetin treatment, four genes showed significantly different expression changes (<jats:italic>C1GALT1</jats:italic>, <jats:italic>O</jats:italic>-glycan biosynthesis; <jats:italic>GM2A</jats:italic>, glycolipid catabolism; <jats:italic>HDGF</jats:italic>, cell proliferation; <jats:italic>SERPINB9</jats:italic>, apoptosis). However, these were minimal in respect to magnitude of fold change. In conclusion, although microarray analysis revealed extensive effects of quercetin on gene expression, the employment of a placebo-controlled study design showed no comparable results for twenty-four verification targets. This emphasises the need for stringent designs in nutritional intervention studies with the aim to identify relevant changes in gene expression.</jats:p> Quercetin supplementation and its effect on human monocyte gene expression profiles <i>in vivo</i> British Journal of Nutrition |
| spellingShingle | Boomgaarden, Inka, Egert, Sarah, Rimbach, Gerald, Wolffram, Siegfried, Müller, Manfred J., Döring, Frank, British Journal of Nutrition, Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo, Nutrition and Dietetics, Medicine (miscellaneous) |
| title | Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo |
| title_full | Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo |
| title_fullStr | Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo |
| title_full_unstemmed | Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo |
| title_short | Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo |
| title_sort | quercetin supplementation and its effect on human monocyte gene expression profiles <i>in vivo</i> |
| title_unstemmed | Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo |
| topic | Nutrition and Dietetics, Medicine (miscellaneous) |
| url | http://dx.doi.org/10.1017/s0007114510000711 |