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8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress
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| Zeitschriftentitel: | FEBS Letters |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | FEBS Letters, 556, 2004, 1-3, S. 104-110 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Kim, Ja-Eun Choi, Seongwon Yoo, Ju-Ah Chung, Myung-Hee Kim, Ja-Eun Choi, Seongwon Yoo, Ju-Ah Chung, Myung-Hee |
|---|---|
| author |
Kim, Ja-Eun Choi, Seongwon Yoo, Ju-Ah Chung, Myung-Hee |
| spellingShingle |
Kim, Ja-Eun Choi, Seongwon Yoo, Ju-Ah Chung, Myung-Hee FEBS Letters 8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics |
| author_sort |
kim, ja-eun |
| spelling |
Kim, Ja-Eun Choi, Seongwon Yoo, Ju-Ah Chung, Myung-Hee 0014-5793 1873-3468 Wiley Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics http://dx.doi.org/10.1016/s0014-5793(03)01385-1 <jats:p>7,8‐Dihydro‐8‐oxoguanine (8‐oxoguanine; 8‐oxo‐G), one of the major oxidative DNA adducts, is highly susceptible to further oxidation by radicals. We confirmed the higher reactivity of 8‐oxo‐G toward reactive oxygen (singlet oxygen and hydroxyl radical) or nitrogen (peroxynitrite) species as compared to unmodified base. In this study, we raised the question about the effect of this high reactivity toward radicals on intramolecular and intermolecular DNA damage. We found that the amount of intact nucleoside in oligodeoxynucleotide containing 8‐oxo‐G decreased more by various radicals at higher levels of 8‐oxo‐G incorporation, and that the oligodeoxynucleotide damage and plasmid cleavage by hydroxyl radical were inhibited in the presence of 7,8‐dihydro‐8‐oxo‐2′‐deoxyguanosine (8‐oxo‐dG). We conclude that 8‐oxo‐G within DNA induces intramolecular DNA base damage, but that free 8‐oxo‐G protects intermolecular DNA from oxidative stress. These results suggest that 8‐oxo‐G within DNA must be rapidly released to protect DNA from overall oxidative damage.</jats:p> 8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress FEBS Letters |
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10.1016/s0014-5793(03)01385-1 |
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Wiley, 2004 |
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Wiley, 2004 |
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| title |
8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress |
| title_unstemmed |
8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress |
| title_full |
8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress |
| title_fullStr |
8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress |
| title_full_unstemmed |
8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress |
| title_short |
8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress |
| title_sort |
8‐oxoguanine induces intramolecular dna damage but free 8‐oxoguanine protects intermolecular dna from oxidative stress |
| topic |
Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics |
| url |
http://dx.doi.org/10.1016/s0014-5793(03)01385-1 |
| publishDate |
2004 |
| physical |
104-110 |
| description |
<jats:p>7,8‐Dihydro‐8‐oxoguanine (8‐oxoguanine; 8‐oxo‐G), one of the major oxidative DNA adducts, is highly susceptible to further oxidation by radicals. We confirmed the higher reactivity of 8‐oxo‐G toward reactive oxygen (singlet oxygen and hydroxyl radical) or nitrogen (peroxynitrite) species as compared to unmodified base. In this study, we raised the question about the effect of this high reactivity toward radicals on intramolecular and intermolecular DNA damage. We found that the amount of intact nucleoside in oligodeoxynucleotide containing 8‐oxo‐G decreased more by various radicals at higher levels of 8‐oxo‐G incorporation, and that the oligodeoxynucleotide damage and plasmid cleavage by hydroxyl radical were inhibited in the presence of 7,8‐dihydro‐8‐oxo‐2′‐deoxyguanosine (8‐oxo‐dG). We conclude that 8‐oxo‐G within DNA induces intramolecular DNA base damage, but that free 8‐oxo‐G protects intermolecular DNA from oxidative stress. These results suggest that 8‐oxo‐G within DNA must be rapidly released to protect DNA from overall oxidative damage.</jats:p> |
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| author | Kim, Ja-Eun, Choi, Seongwon, Yoo, Ju-Ah, Chung, Myung-Hee |
| author_facet | Kim, Ja-Eun, Choi, Seongwon, Yoo, Ju-Ah, Chung, Myung-Hee, Kim, Ja-Eun, Choi, Seongwon, Yoo, Ju-Ah, Chung, Myung-Hee |
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| description | <jats:p>7,8‐Dihydro‐8‐oxoguanine (8‐oxoguanine; 8‐oxo‐G), one of the major oxidative DNA adducts, is highly susceptible to further oxidation by radicals. We confirmed the higher reactivity of 8‐oxo‐G toward reactive oxygen (singlet oxygen and hydroxyl radical) or nitrogen (peroxynitrite) species as compared to unmodified base. In this study, we raised the question about the effect of this high reactivity toward radicals on intramolecular and intermolecular DNA damage. We found that the amount of intact nucleoside in oligodeoxynucleotide containing 8‐oxo‐G decreased more by various radicals at higher levels of 8‐oxo‐G incorporation, and that the oligodeoxynucleotide damage and plasmid cleavage by hydroxyl radical were inhibited in the presence of 7,8‐dihydro‐8‐oxo‐2′‐deoxyguanosine (8‐oxo‐dG). We conclude that 8‐oxo‐G within DNA induces intramolecular DNA base damage, but that free 8‐oxo‐G protects intermolecular DNA from oxidative stress. These results suggest that 8‐oxo‐G within DNA must be rapidly released to protect DNA from overall oxidative damage.</jats:p> |
| doi_str_mv | 10.1016/s0014-5793(03)01385-1 |
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| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAxNi9zMDAxNC01NzkzKDAzKTAxMzg1LTE |
| imprint | Wiley, 2004 |
| imprint_str_mv | Wiley, 2004 |
| institution | DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1 |
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| physical | 104-110 |
| publishDate | 2004 |
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| spelling | Kim, Ja-Eun Choi, Seongwon Yoo, Ju-Ah Chung, Myung-Hee 0014-5793 1873-3468 Wiley Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics http://dx.doi.org/10.1016/s0014-5793(03)01385-1 <jats:p>7,8‐Dihydro‐8‐oxoguanine (8‐oxoguanine; 8‐oxo‐G), one of the major oxidative DNA adducts, is highly susceptible to further oxidation by radicals. We confirmed the higher reactivity of 8‐oxo‐G toward reactive oxygen (singlet oxygen and hydroxyl radical) or nitrogen (peroxynitrite) species as compared to unmodified base. In this study, we raised the question about the effect of this high reactivity toward radicals on intramolecular and intermolecular DNA damage. We found that the amount of intact nucleoside in oligodeoxynucleotide containing 8‐oxo‐G decreased more by various radicals at higher levels of 8‐oxo‐G incorporation, and that the oligodeoxynucleotide damage and plasmid cleavage by hydroxyl radical were inhibited in the presence of 7,8‐dihydro‐8‐oxo‐2′‐deoxyguanosine (8‐oxo‐dG). We conclude that 8‐oxo‐G within DNA induces intramolecular DNA base damage, but that free 8‐oxo‐G protects intermolecular DNA from oxidative stress. These results suggest that 8‐oxo‐G within DNA must be rapidly released to protect DNA from overall oxidative damage.</jats:p> 8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress FEBS Letters |
| spellingShingle | Kim, Ja-Eun, Choi, Seongwon, Yoo, Ju-Ah, Chung, Myung-Hee, FEBS Letters, 8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress, Cell Biology, Genetics, Molecular Biology, Biochemistry, Structural Biology, Biophysics |
| title | 8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress |
| title_full | 8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress |
| title_fullStr | 8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress |
| title_full_unstemmed | 8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress |
| title_short | 8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress |
| title_sort | 8‐oxoguanine induces intramolecular dna damage but free 8‐oxoguanine protects intermolecular dna from oxidative stress |
| title_unstemmed | 8‐Oxoguanine induces intramolecular DNA damage but free 8‐oxoguanine protects intermolecular DNA from oxidative stress |
| topic | Cell Biology, Genetics, Molecular Biology, Biochemistry, Structural Biology, Biophysics |
| url | http://dx.doi.org/10.1016/s0014-5793(03)01385-1 |