Eintrag weiter verarbeiten
Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes
Gespeichert in:
| Zeitschriftentitel: | International Journal of Developmental Neuroscience |
|---|---|
| Personen und Körperschaften: | , |
| In: | International Journal of Developmental Neuroscience, 7, 1989, 2, S. 165-172 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Brodie, Chaya Sampson, S. R. Brodie, Chaya Sampson, S. R. |
|---|---|
| author |
Brodie, Chaya Sampson, S. R. |
| spellingShingle |
Brodie, Chaya Sampson, S. R. International Journal of Developmental Neuroscience Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes Developmental Biology Developmental Neuroscience |
| author_sort |
brodie, chaya |
| spelling |
Brodie, Chaya Sampson, S. R. 0736-5748 1873-474X Wiley Developmental Biology Developmental Neuroscience http://dx.doi.org/10.1016/0736-5748(89)90066-x <jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>The role of the electrogenic Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump in the determination of the level of the resting membrane potential in cultured chick limb muscle was investigated. Transmembrane resting potential and ouabain‐sensitive<jats:sup>86</jats:sup>Rb‐uptake were measured in myotubes at different ages in culture from 2 to 10 days<jats:italic>in vitro</jats:italic>. Inhibition of the Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump with ouabain prevented the developmental increase in membrane potential which normally follows fusion of myotubes (day 2–3). In mature myotubes, ouabain caused a dose‐related decrease in both membrane potential and<jats:sup>86</jats:sup>Rb‐uptake, with values for EC<jats:sub>50</jats:sub> and maximal effect being nearly the same on both variables. The decrease in membrane potential by ouabain, up to 20 mV maximum, occurred within 2–5 sec and was not accompanied by detectable changes in input resistance. Membrane potential was also reduced by a decrease in temperature of the recording medium and removal of extracellular K<jats:sup>+</jats:sup>, both of which reduce Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump activity. We also found that the relation between membrane potential and extracellular K<jats:sup>+</jats:sup> concentration was completely attenuated by ouabain in the physiological range (2–10 mM). We conclude that the electrogenic Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump plays an important role in the determination of the resting membrane potential of chick myotubes and that regulation of its level is not entirely explained by the diffusion potential hypothesis.</jats:p></jats:sec> Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes International Journal of Developmental Neuroscience |
| doi_str_mv |
10.1016/0736-5748(89)90066-x |
| facet_avail |
Online |
| finc_class_facet |
Biologie Psychologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAxNi8wNzM2LTU3NDgoODkpOTAwNjYteA |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAxNi8wNzM2LTU3NDgoODkpOTAwNjYteA |
| institution |
DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 |
| imprint |
Wiley, 1989 |
| imprint_str_mv |
Wiley, 1989 |
| issn |
1873-474X 0736-5748 |
| issn_str_mv |
1873-474X 0736-5748 |
| language |
English |
| mega_collection |
Wiley (CrossRef) |
| match_str |
brodie1989characterizationofrestingmembranepotentialanditselectrogenicpumpcomponentinculturedchickmyotubes |
| publishDateSort |
1989 |
| publisher |
Wiley |
| recordtype |
ai |
| record_format |
ai |
| series |
International Journal of Developmental Neuroscience |
| source_id |
49 |
| title |
Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| title_unstemmed |
Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| title_full |
Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| title_fullStr |
Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| title_full_unstemmed |
Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| title_short |
Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| title_sort |
characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| topic |
Developmental Biology Developmental Neuroscience |
| url |
http://dx.doi.org/10.1016/0736-5748(89)90066-x |
| publishDate |
1989 |
| physical |
165-172 |
| description |
<jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>The role of the electrogenic Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump in the determination of the level of the resting membrane potential in cultured chick limb muscle was investigated. Transmembrane resting potential and ouabain‐sensitive<jats:sup>86</jats:sup>Rb‐uptake were measured in myotubes at different ages in culture from 2 to 10 days<jats:italic>in vitro</jats:italic>. Inhibition of the Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump with ouabain prevented the developmental increase in membrane potential which normally follows fusion of myotubes (day 2–3). In mature myotubes, ouabain caused a dose‐related decrease in both membrane potential and<jats:sup>86</jats:sup>Rb‐uptake, with values for EC<jats:sub>50</jats:sub> and maximal effect being nearly the same on both variables. The decrease in membrane potential by ouabain, up to 20 mV maximum, occurred within 2–5 sec and was not accompanied by detectable changes in input resistance. Membrane potential was also reduced by a decrease in temperature of the recording medium and removal of extracellular K<jats:sup>+</jats:sup>, both of which reduce Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump activity. We also found that the relation between membrane potential and extracellular K<jats:sup>+</jats:sup> concentration was completely attenuated by ouabain in the physiological range (2–10 mM). We conclude that the electrogenic Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump plays an important role in the determination of the resting membrane potential of chick myotubes and that regulation of its level is not entirely explained by the diffusion potential hypothesis.</jats:p></jats:sec> |
| container_issue |
2 |
| container_start_page |
165 |
| container_title |
International Journal of Developmental Neuroscience |
| container_volume |
7 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792336699215314946 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T15:04:36.096Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Characterization+of+resting+membrane+potential+and+its+electrogenic+pump+component+in+cultured+chick+myotubes&rft.date=1989-01-01&genre=article&issn=1873-474X&volume=7&issue=2&spage=165&epage=172&pages=165-172&jtitle=International+Journal+of+Developmental+Neuroscience&atitle=Characterization+of+resting+membrane+potential+and+its+electrogenic+pump+component+in+cultured+chick+myotubes&aulast=Sampson&aufirst=S.+R.&rft_id=info%3Adoi%2F10.1016%2F0736-5748%2889%2990066-x&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792336699215314946 |
| author | Brodie, Chaya, Sampson, S. R. |
| author_facet | Brodie, Chaya, Sampson, S. R., Brodie, Chaya, Sampson, S. R. |
| author_sort | brodie, chaya |
| container_issue | 2 |
| container_start_page | 165 |
| container_title | International Journal of Developmental Neuroscience |
| container_volume | 7 |
| description | <jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>The role of the electrogenic Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump in the determination of the level of the resting membrane potential in cultured chick limb muscle was investigated. Transmembrane resting potential and ouabain‐sensitive<jats:sup>86</jats:sup>Rb‐uptake were measured in myotubes at different ages in culture from 2 to 10 days<jats:italic>in vitro</jats:italic>. Inhibition of the Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump with ouabain prevented the developmental increase in membrane potential which normally follows fusion of myotubes (day 2–3). In mature myotubes, ouabain caused a dose‐related decrease in both membrane potential and<jats:sup>86</jats:sup>Rb‐uptake, with values for EC<jats:sub>50</jats:sub> and maximal effect being nearly the same on both variables. The decrease in membrane potential by ouabain, up to 20 mV maximum, occurred within 2–5 sec and was not accompanied by detectable changes in input resistance. Membrane potential was also reduced by a decrease in temperature of the recording medium and removal of extracellular K<jats:sup>+</jats:sup>, both of which reduce Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump activity. We also found that the relation between membrane potential and extracellular K<jats:sup>+</jats:sup> concentration was completely attenuated by ouabain in the physiological range (2–10 mM). We conclude that the electrogenic Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump plays an important role in the determination of the resting membrane potential of chick myotubes and that regulation of its level is not entirely explained by the diffusion potential hypothesis.</jats:p></jats:sec> |
| doi_str_mv | 10.1016/0736-5748(89)90066-x |
| facet_avail | Online |
| finc_class_facet | Biologie, Psychologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAxNi8wNzM2LTU3NDgoODkpOTAwNjYteA |
| imprint | Wiley, 1989 |
| imprint_str_mv | Wiley, 1989 |
| institution | DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
| issn | 1873-474X, 0736-5748 |
| issn_str_mv | 1873-474X, 0736-5748 |
| language | English |
| last_indexed | 2024-03-01T15:04:36.096Z |
| match_str | brodie1989characterizationofrestingmembranepotentialanditselectrogenicpumpcomponentinculturedchickmyotubes |
| mega_collection | Wiley (CrossRef) |
| physical | 165-172 |
| publishDate | 1989 |
| publishDateSort | 1989 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | International Journal of Developmental Neuroscience |
| source_id | 49 |
| spelling | Brodie, Chaya Sampson, S. R. 0736-5748 1873-474X Wiley Developmental Biology Developmental Neuroscience http://dx.doi.org/10.1016/0736-5748(89)90066-x <jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>The role of the electrogenic Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump in the determination of the level of the resting membrane potential in cultured chick limb muscle was investigated. Transmembrane resting potential and ouabain‐sensitive<jats:sup>86</jats:sup>Rb‐uptake were measured in myotubes at different ages in culture from 2 to 10 days<jats:italic>in vitro</jats:italic>. Inhibition of the Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump with ouabain prevented the developmental increase in membrane potential which normally follows fusion of myotubes (day 2–3). In mature myotubes, ouabain caused a dose‐related decrease in both membrane potential and<jats:sup>86</jats:sup>Rb‐uptake, with values for EC<jats:sub>50</jats:sub> and maximal effect being nearly the same on both variables. The decrease in membrane potential by ouabain, up to 20 mV maximum, occurred within 2–5 sec and was not accompanied by detectable changes in input resistance. Membrane potential was also reduced by a decrease in temperature of the recording medium and removal of extracellular K<jats:sup>+</jats:sup>, both of which reduce Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump activity. We also found that the relation between membrane potential and extracellular K<jats:sup>+</jats:sup> concentration was completely attenuated by ouabain in the physiological range (2–10 mM). We conclude that the electrogenic Na<jats:sup>+</jats:sup>‐K<jats:sup>+</jats:sup> pump plays an important role in the determination of the resting membrane potential of chick myotubes and that regulation of its level is not entirely explained by the diffusion potential hypothesis.</jats:p></jats:sec> Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes International Journal of Developmental Neuroscience |
| spellingShingle | Brodie, Chaya, Sampson, S. R., International Journal of Developmental Neuroscience, Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes, Developmental Biology, Developmental Neuroscience |
| title | Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| title_full | Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| title_fullStr | Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| title_full_unstemmed | Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| title_short | Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| title_sort | characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| title_unstemmed | Characterization of resting membrane potential and its electrogenic pump component in cultured chick myotubes |
| topic | Developmental Biology, Developmental Neuroscience |
| url | http://dx.doi.org/10.1016/0736-5748(89)90066-x |