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Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli
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| Zeitschriftentitel: | FEBS Letters |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | FEBS Letters, 307, 1992, 2, S. 173-176 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Ivanov, I. Alexandrova, R. Dragulev, B. Saraffova, A. AbouHaidar, M.G. Ivanov, I. Alexandrova, R. Dragulev, B. Saraffova, A. AbouHaidar, M.G. |
|---|---|
| author |
Ivanov, I. Alexandrova, R. Dragulev, B. Saraffova, A. AbouHaidar, M.G. |
| spellingShingle |
Ivanov, I. Alexandrova, R. Dragulev, B. Saraffova, A. AbouHaidar, M.G. FEBS Letters Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics |
| author_sort |
ivanov, i. |
| spelling |
Ivanov, I. Alexandrova, R. Dragulev, B. Saraffova, A. AbouHaidar, M.G. 0014-5793 1873-3468 Wiley Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics http://dx.doi.org/10.1016/0014-5793(92)80761-5 <jats:p>It has been shown that tandems of rare arginine codons AGG have a strong inhibitory effect on translation of mRNA in <jats:italic>E. coli</jats:italic> [5]. This has been explained by the rate‐limiting interaction of these codons with the less abundant tRNA<jats:sup>AGG</jats:sup> [6]. In this study tandemly repeated AGG triplets were introduced into the chloramphenicol acetyltransferase (CAT) gene either upstream of the initiation ATG codon or downstream of it (both in frame and out of frame) and the expression of the modified genes was investigated. We report that the addition of AGG clusters resulted in a substantial inhibitory effect on CAT gene expression independently of their localization in mRNA. This inhibitory effect is explained by a competition of the tandem AGGAGG with the natural Shine—Dalgarno (SD) sequence (consensus AAGGAGGU) for the 3′‐end of the 16S small ribosomal RNA (rRNA).</jats:p> Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in <i>E. coli</i> FEBS Letters |
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10.1016/0014-5793(92)80761-5 |
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Wiley, 1992 |
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Wiley, 1992 |
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FEBS Letters |
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| title |
Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli |
| title_unstemmed |
Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli |
| title_full |
Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli |
| title_fullStr |
Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli |
| title_full_unstemmed |
Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli |
| title_short |
Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli |
| title_sort |
effect of tandemly repeated agg triplets on the translation of cat‐mrna in <i>e. coli</i> |
| topic |
Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics |
| url |
http://dx.doi.org/10.1016/0014-5793(92)80761-5 |
| publishDate |
1992 |
| physical |
173-176 |
| description |
<jats:p>It has been shown that tandems of rare arginine codons AGG have a strong inhibitory effect on translation of mRNA in <jats:italic>E. coli</jats:italic> [5]. This has been explained by the rate‐limiting interaction of these codons with the less abundant tRNA<jats:sup>AGG</jats:sup> [6]. In this study tandemly repeated AGG triplets were introduced into the chloramphenicol acetyltransferase (CAT) gene either upstream of the initiation ATG codon or downstream of it (both in frame and out of frame) and the expression of the modified genes was investigated. We report that the addition of AGG clusters resulted in a substantial inhibitory effect on CAT gene expression independently of their localization in mRNA. This inhibitory effect is explained by a competition of the tandem AGGAGG with the natural Shine—Dalgarno (SD) sequence (consensus AAGGAGGU) for the 3′‐end of the 16S small ribosomal RNA (rRNA).</jats:p> |
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| author | Ivanov, I., Alexandrova, R., Dragulev, B., Saraffova, A., AbouHaidar, M.G. |
| author_facet | Ivanov, I., Alexandrova, R., Dragulev, B., Saraffova, A., AbouHaidar, M.G., Ivanov, I., Alexandrova, R., Dragulev, B., Saraffova, A., AbouHaidar, M.G. |
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| description | <jats:p>It has been shown that tandems of rare arginine codons AGG have a strong inhibitory effect on translation of mRNA in <jats:italic>E. coli</jats:italic> [5]. This has been explained by the rate‐limiting interaction of these codons with the less abundant tRNA<jats:sup>AGG</jats:sup> [6]. In this study tandemly repeated AGG triplets were introduced into the chloramphenicol acetyltransferase (CAT) gene either upstream of the initiation ATG codon or downstream of it (both in frame and out of frame) and the expression of the modified genes was investigated. We report that the addition of AGG clusters resulted in a substantial inhibitory effect on CAT gene expression independently of their localization in mRNA. This inhibitory effect is explained by a competition of the tandem AGGAGG with the natural Shine—Dalgarno (SD) sequence (consensus AAGGAGGU) for the 3′‐end of the 16S small ribosomal RNA (rRNA).</jats:p> |
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| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAxNi8wMDE0LTU3OTMoOTIpODA3NjEtNQ |
| imprint | Wiley, 1992 |
| imprint_str_mv | Wiley, 1992 |
| institution | DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
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| physical | 173-176 |
| publishDate | 1992 |
| publishDateSort | 1992 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | FEBS Letters |
| source_id | 49 |
| spelling | Ivanov, I. Alexandrova, R. Dragulev, B. Saraffova, A. AbouHaidar, M.G. 0014-5793 1873-3468 Wiley Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics http://dx.doi.org/10.1016/0014-5793(92)80761-5 <jats:p>It has been shown that tandems of rare arginine codons AGG have a strong inhibitory effect on translation of mRNA in <jats:italic>E. coli</jats:italic> [5]. This has been explained by the rate‐limiting interaction of these codons with the less abundant tRNA<jats:sup>AGG</jats:sup> [6]. In this study tandemly repeated AGG triplets were introduced into the chloramphenicol acetyltransferase (CAT) gene either upstream of the initiation ATG codon or downstream of it (both in frame and out of frame) and the expression of the modified genes was investigated. We report that the addition of AGG clusters resulted in a substantial inhibitory effect on CAT gene expression independently of their localization in mRNA. This inhibitory effect is explained by a competition of the tandem AGGAGG with the natural Shine—Dalgarno (SD) sequence (consensus AAGGAGGU) for the 3′‐end of the 16S small ribosomal RNA (rRNA).</jats:p> Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in <i>E. coli</i> FEBS Letters |
| spellingShingle | Ivanov, I., Alexandrova, R., Dragulev, B., Saraffova, A., AbouHaidar, M.G., FEBS Letters, Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli, Cell Biology, Genetics, Molecular Biology, Biochemistry, Structural Biology, Biophysics |
| title | Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli |
| title_full | Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli |
| title_fullStr | Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli |
| title_full_unstemmed | Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli |
| title_short | Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli |
| title_sort | effect of tandemly repeated agg triplets on the translation of cat‐mrna in <i>e. coli</i> |
| title_unstemmed | Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli |
| topic | Cell Biology, Genetics, Molecular Biology, Biochemistry, Structural Biology, Biophysics |
| url | http://dx.doi.org/10.1016/0014-5793(92)80761-5 |