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Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study
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| Zeitschriftentitel: | Synapse |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Synapse, 53, 2004, 3, S. 159-167 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Fusco, F.R. Martorana, A. Giampà, C. De March, Z. Farini, D. D'Angelo, V. Sancesario, G. Bernardi, G. Fusco, F.R. Martorana, A. Giampà, C. De March, Z. Farini, D. D'Angelo, V. Sancesario, G. Bernardi, G. |
|---|---|
| author |
Fusco, F.R. Martorana, A. Giampà, C. De March, Z. Farini, D. D'Angelo, V. Sancesario, G. Bernardi, G. |
| spellingShingle |
Fusco, F.R. Martorana, A. Giampà, C. De March, Z. Farini, D. D'Angelo, V. Sancesario, G. Bernardi, G. Synapse Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study Cellular and Molecular Neuroscience |
| author_sort |
fusco, f.r. |
| spelling |
Fusco, F.R. Martorana, A. Giampà, C. De March, Z. Farini, D. D'Angelo, V. Sancesario, G. Bernardi, G. 0887-4476 1098-2396 Wiley Cellular and Molecular Neuroscience http://dx.doi.org/10.1002/syn.20047 <jats:title>Abstract</jats:title><jats:p>Several lines of evidence indicate that cannabinoids, among other functions, are involved in motor control. Although cannabinoid receptors (CB<jats:sub>1</jats:sub>) mRNA has been observed in medium‐sized spiny neurons of the striatum, a description of the precise localization of CB<jats:sub>1</jats:sub> at a protein level among striatal cells is still lacking. Therefore, we performed immunohistochemical studies with light and confocal microscopy to identify neuronal subpopulations that express CB<jats:sub>1</jats:sub> and to assess the distribution of the receptor within these neurons. In our single label light microscopy study, CB<jats:sub>1</jats:sub> was observed in most medium‐sized neurons of the caudate‐putamen. However, CB<jats:sub>1</jats:sub> was also present in large‐sized neurons scattered throughout the striatum. Our dual‐label study showed that 89.3% of projection neurons in matrix contain CB<jats:sub>1</jats:sub>, and that 56.4% of projection neurons in patch are labeled for CB<jats:sub>1</jats:sub>. To investigate the presence of CB<jats:sub>1</jats:sub> among the different subclasses of striatal interneurons we performed a double‐labeling study matching CB<jats:sub>1</jats:sub> and each of the striatal interneuron markers, namely, choline acetyl‐transferase, parvalbumin, calretinin, and nitric oxide synthase. Our double‐label study showed that most parvalbumin immunoreactive interneurons (86.5%), more than one‐third (39.2%) of cholinergic interneurons, and about one‐third (30.4%) of the NOS‐positive neurons are labeled for CB<jats:sub>1</jats:sub>. Calretinin‐immunolabeled neurons were devoid of CB<jats:sub>1</jats:sub>. Synapse 53:159–167, 2004. © 2004 Wiley‐Liss, Inc.</jats:p> Immunolocalization of CB<sub>1</sub> receptor in rat striatal neurons: A confocal microscopy study Synapse |
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| title |
Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study |
| title_unstemmed |
Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study |
| title_full |
Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study |
| title_fullStr |
Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study |
| title_full_unstemmed |
Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study |
| title_short |
Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study |
| title_sort |
immunolocalization of cb<sub>1</sub> receptor in rat striatal neurons: a confocal microscopy study |
| topic |
Cellular and Molecular Neuroscience |
| url |
http://dx.doi.org/10.1002/syn.20047 |
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2004 |
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159-167 |
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<jats:title>Abstract</jats:title><jats:p>Several lines of evidence indicate that cannabinoids, among other functions, are involved in motor control. Although cannabinoid receptors (CB<jats:sub>1</jats:sub>) mRNA has been observed in medium‐sized spiny neurons of the striatum, a description of the precise localization of CB<jats:sub>1</jats:sub> at a protein level among striatal cells is still lacking. Therefore, we performed immunohistochemical studies with light and confocal microscopy to identify neuronal subpopulations that express CB<jats:sub>1</jats:sub> and to assess the distribution of the receptor within these neurons. In our single label light microscopy study, CB<jats:sub>1</jats:sub> was observed in most medium‐sized neurons of the caudate‐putamen. However, CB<jats:sub>1</jats:sub> was also present in large‐sized neurons scattered throughout the striatum. Our dual‐label study showed that 89.3% of projection neurons in matrix contain CB<jats:sub>1</jats:sub>, and that 56.4% of projection neurons in patch are labeled for CB<jats:sub>1</jats:sub>. To investigate the presence of CB<jats:sub>1</jats:sub> among the different subclasses of striatal interneurons we performed a double‐labeling study matching CB<jats:sub>1</jats:sub> and each of the striatal interneuron markers, namely, choline acetyl‐transferase, parvalbumin, calretinin, and nitric oxide synthase. Our double‐label study showed that most parvalbumin immunoreactive interneurons (86.5%), more than one‐third (39.2%) of cholinergic interneurons, and about one‐third (30.4%) of the NOS‐positive neurons are labeled for CB<jats:sub>1</jats:sub>. Calretinin‐immunolabeled neurons were devoid of CB<jats:sub>1</jats:sub>. Synapse 53:159–167, 2004. © 2004 Wiley‐Liss, Inc.</jats:p> |
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| description | <jats:title>Abstract</jats:title><jats:p>Several lines of evidence indicate that cannabinoids, among other functions, are involved in motor control. Although cannabinoid receptors (CB<jats:sub>1</jats:sub>) mRNA has been observed in medium‐sized spiny neurons of the striatum, a description of the precise localization of CB<jats:sub>1</jats:sub> at a protein level among striatal cells is still lacking. Therefore, we performed immunohistochemical studies with light and confocal microscopy to identify neuronal subpopulations that express CB<jats:sub>1</jats:sub> and to assess the distribution of the receptor within these neurons. In our single label light microscopy study, CB<jats:sub>1</jats:sub> was observed in most medium‐sized neurons of the caudate‐putamen. However, CB<jats:sub>1</jats:sub> was also present in large‐sized neurons scattered throughout the striatum. Our dual‐label study showed that 89.3% of projection neurons in matrix contain CB<jats:sub>1</jats:sub>, and that 56.4% of projection neurons in patch are labeled for CB<jats:sub>1</jats:sub>. To investigate the presence of CB<jats:sub>1</jats:sub> among the different subclasses of striatal interneurons we performed a double‐labeling study matching CB<jats:sub>1</jats:sub> and each of the striatal interneuron markers, namely, choline acetyl‐transferase, parvalbumin, calretinin, and nitric oxide synthase. Our double‐label study showed that most parvalbumin immunoreactive interneurons (86.5%), more than one‐third (39.2%) of cholinergic interneurons, and about one‐third (30.4%) of the NOS‐positive neurons are labeled for CB<jats:sub>1</jats:sub>. Calretinin‐immunolabeled neurons were devoid of CB<jats:sub>1</jats:sub>. Synapse 53:159–167, 2004. © 2004 Wiley‐Liss, Inc.</jats:p> |
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| spelling | Fusco, F.R. Martorana, A. Giampà, C. De March, Z. Farini, D. D'Angelo, V. Sancesario, G. Bernardi, G. 0887-4476 1098-2396 Wiley Cellular and Molecular Neuroscience http://dx.doi.org/10.1002/syn.20047 <jats:title>Abstract</jats:title><jats:p>Several lines of evidence indicate that cannabinoids, among other functions, are involved in motor control. Although cannabinoid receptors (CB<jats:sub>1</jats:sub>) mRNA has been observed in medium‐sized spiny neurons of the striatum, a description of the precise localization of CB<jats:sub>1</jats:sub> at a protein level among striatal cells is still lacking. Therefore, we performed immunohistochemical studies with light and confocal microscopy to identify neuronal subpopulations that express CB<jats:sub>1</jats:sub> and to assess the distribution of the receptor within these neurons. In our single label light microscopy study, CB<jats:sub>1</jats:sub> was observed in most medium‐sized neurons of the caudate‐putamen. However, CB<jats:sub>1</jats:sub> was also present in large‐sized neurons scattered throughout the striatum. Our dual‐label study showed that 89.3% of projection neurons in matrix contain CB<jats:sub>1</jats:sub>, and that 56.4% of projection neurons in patch are labeled for CB<jats:sub>1</jats:sub>. To investigate the presence of CB<jats:sub>1</jats:sub> among the different subclasses of striatal interneurons we performed a double‐labeling study matching CB<jats:sub>1</jats:sub> and each of the striatal interneuron markers, namely, choline acetyl‐transferase, parvalbumin, calretinin, and nitric oxide synthase. Our double‐label study showed that most parvalbumin immunoreactive interneurons (86.5%), more than one‐third (39.2%) of cholinergic interneurons, and about one‐third (30.4%) of the NOS‐positive neurons are labeled for CB<jats:sub>1</jats:sub>. Calretinin‐immunolabeled neurons were devoid of CB<jats:sub>1</jats:sub>. Synapse 53:159–167, 2004. © 2004 Wiley‐Liss, Inc.</jats:p> Immunolocalization of CB<sub>1</sub> receptor in rat striatal neurons: A confocal microscopy study Synapse |
| spellingShingle | Fusco, F.R., Martorana, A., Giampà, C., De March, Z., Farini, D., D'Angelo, V., Sancesario, G., Bernardi, G., Synapse, Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study, Cellular and Molecular Neuroscience |
| title | Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study |
| title_full | Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study |
| title_fullStr | Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study |
| title_full_unstemmed | Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study |
| title_short | Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study |
| title_sort | immunolocalization of cb<sub>1</sub> receptor in rat striatal neurons: a confocal microscopy study |
| title_unstemmed | Immunolocalization of CB1 receptor in rat striatal neurons: A confocal microscopy study |
| topic | Cellular and Molecular Neuroscience |
| url | http://dx.doi.org/10.1002/syn.20047 |