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Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway
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| Zeitschriftentitel: | Stem Cells |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Stem Cells, 32, 2014, 1, S. 166-176 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Oxford University Press (OUP)
|
| Schlagwörter: |
| author_facet |
Wu, Haibo Wu, Yongyan Ai, Zhiying Yang, Liping Gao, Yuan Du, Juan Guo, Zekun Zhang, Yong Wu, Haibo Wu, Yongyan Ai, Zhiying Yang, Liping Gao, Yuan Du, Juan Guo, Zekun Zhang, Yong |
|---|---|
| author |
Wu, Haibo Wu, Yongyan Ai, Zhiying Yang, Liping Gao, Yuan Du, Juan Guo, Zekun Zhang, Yong |
| spellingShingle |
Wu, Haibo Wu, Yongyan Ai, Zhiying Yang, Liping Gao, Yuan Du, Juan Guo, Zekun Zhang, Yong Stem Cells Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway Cell Biology Developmental Biology Molecular Medicine |
| author_sort |
wu, haibo |
| spelling |
Wu, Haibo Wu, Yongyan Ai, Zhiying Yang, Liping Gao, Yuan Du, Juan Guo, Zekun Zhang, Yong 1066-5099 1549-4918 Oxford University Press (OUP) Cell Biology Developmental Biology Molecular Medicine http://dx.doi.org/10.1002/stem.1523 <jats:title>Abstract</jats:title> <jats:p>Vitamin C (Vc), also known as ascorbic acid, is involved in many important metabolic and physiological reactions in the body. Here, we report that Vc enhances the expression of Nanog and inhibits retinoic acid-induced differentiation of embryonic stem cells. We investigated Vc regulation of Nanog through Janus kinase/signal transducer and activator of transcription pathway using cell signaling pathway profiling systems, and further confirmed by specific pathway inhibition. Using overexpression and knockdown strategies, we demonstrated that STAT2 is a new positive regulator of Nanog and is activated by phosphorylation following Vc treatment. In addition, site mutation analysis identified that STAT2 physically occupies the Nanog promoter, which was confirmed by chromatin immunoprecipitation and electrophoretic mobility shift assays. Taken together, our data suggest a role for Vc in Nanog regulation networks and reveal a novel role for STAT2 in regulating Nanog expression. Stem Cells 2014;32:166–176</jats:p> Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway Stem Cells |
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| title |
Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway |
| title_unstemmed |
Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway |
| title_full |
Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway |
| title_fullStr |
Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway |
| title_full_unstemmed |
Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway |
| title_short |
Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway |
| title_sort |
vitamin c enhances nanog expression via activation of the jak/stat signaling pathway |
| topic |
Cell Biology Developmental Biology Molecular Medicine |
| url |
http://dx.doi.org/10.1002/stem.1523 |
| publishDate |
2014 |
| physical |
166-176 |
| description |
<jats:title>Abstract</jats:title>
<jats:p>Vitamin C (Vc), also known as ascorbic acid, is involved in many important metabolic and physiological reactions in the body. Here, we report that Vc enhances the expression of Nanog and inhibits retinoic acid-induced differentiation of embryonic stem cells. We investigated Vc regulation of Nanog through Janus kinase/signal transducer and activator of transcription pathway using cell signaling pathway profiling systems, and further confirmed by specific pathway inhibition. Using overexpression and knockdown strategies, we demonstrated that STAT2 is a new positive regulator of Nanog and is activated by phosphorylation following Vc treatment. In addition, site mutation analysis identified that STAT2 physically occupies the Nanog promoter, which was confirmed by chromatin immunoprecipitation and electrophoretic mobility shift assays. Taken together, our data suggest a role for Vc in Nanog regulation networks and reveal a novel role for STAT2 in regulating Nanog expression. Stem Cells 2014;32:166–176</jats:p> |
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| author | Wu, Haibo, Wu, Yongyan, Ai, Zhiying, Yang, Liping, Gao, Yuan, Du, Juan, Guo, Zekun, Zhang, Yong |
| author_facet | Wu, Haibo, Wu, Yongyan, Ai, Zhiying, Yang, Liping, Gao, Yuan, Du, Juan, Guo, Zekun, Zhang, Yong, Wu, Haibo, Wu, Yongyan, Ai, Zhiying, Yang, Liping, Gao, Yuan, Du, Juan, Guo, Zekun, Zhang, Yong |
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| description | <jats:title>Abstract</jats:title> <jats:p>Vitamin C (Vc), also known as ascorbic acid, is involved in many important metabolic and physiological reactions in the body. Here, we report that Vc enhances the expression of Nanog and inhibits retinoic acid-induced differentiation of embryonic stem cells. We investigated Vc regulation of Nanog through Janus kinase/signal transducer and activator of transcription pathway using cell signaling pathway profiling systems, and further confirmed by specific pathway inhibition. Using overexpression and knockdown strategies, we demonstrated that STAT2 is a new positive regulator of Nanog and is activated by phosphorylation following Vc treatment. In addition, site mutation analysis identified that STAT2 physically occupies the Nanog promoter, which was confirmed by chromatin immunoprecipitation and electrophoretic mobility shift assays. Taken together, our data suggest a role for Vc in Nanog regulation networks and reveal a novel role for STAT2 in regulating Nanog expression. Stem Cells 2014;32:166–176</jats:p> |
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| imprint_str_mv | Oxford University Press (OUP), 2014 |
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| spelling | Wu, Haibo Wu, Yongyan Ai, Zhiying Yang, Liping Gao, Yuan Du, Juan Guo, Zekun Zhang, Yong 1066-5099 1549-4918 Oxford University Press (OUP) Cell Biology Developmental Biology Molecular Medicine http://dx.doi.org/10.1002/stem.1523 <jats:title>Abstract</jats:title> <jats:p>Vitamin C (Vc), also known as ascorbic acid, is involved in many important metabolic and physiological reactions in the body. Here, we report that Vc enhances the expression of Nanog and inhibits retinoic acid-induced differentiation of embryonic stem cells. We investigated Vc regulation of Nanog through Janus kinase/signal transducer and activator of transcription pathway using cell signaling pathway profiling systems, and further confirmed by specific pathway inhibition. Using overexpression and knockdown strategies, we demonstrated that STAT2 is a new positive regulator of Nanog and is activated by phosphorylation following Vc treatment. In addition, site mutation analysis identified that STAT2 physically occupies the Nanog promoter, which was confirmed by chromatin immunoprecipitation and electrophoretic mobility shift assays. Taken together, our data suggest a role for Vc in Nanog regulation networks and reveal a novel role for STAT2 in regulating Nanog expression. Stem Cells 2014;32:166–176</jats:p> Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway Stem Cells |
| spellingShingle | Wu, Haibo, Wu, Yongyan, Ai, Zhiying, Yang, Liping, Gao, Yuan, Du, Juan, Guo, Zekun, Zhang, Yong, Stem Cells, Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway, Cell Biology, Developmental Biology, Molecular Medicine |
| title | Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway |
| title_full | Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway |
| title_fullStr | Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway |
| title_full_unstemmed | Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway |
| title_short | Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway |
| title_sort | vitamin c enhances nanog expression via activation of the jak/stat signaling pathway |
| title_unstemmed | Vitamin C Enhances Nanog Expression Via Activation of the JAK/STAT Signaling Pathway |
| topic | Cell Biology, Developmental Biology, Molecular Medicine |
| url | http://dx.doi.org/10.1002/stem.1523 |