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Use of monoclonal antibodies for the analysis of mycotoxins
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| Zeitschriftentitel: | Natural Toxins |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Natural Toxins, 3, 1995, 4, S. 288-293 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Dietrich, Richard Schneider, Elisabeth Usleber, Ewald Märtibauer, Erwin Dietrich, Richard Schneider, Elisabeth Usleber, Ewald Märtibauer, Erwin |
|---|---|
| author |
Dietrich, Richard Schneider, Elisabeth Usleber, Ewald Märtibauer, Erwin |
| spellingShingle |
Dietrich, Richard Schneider, Elisabeth Usleber, Ewald Märtibauer, Erwin Natural Toxins Use of monoclonal antibodies for the analysis of mycotoxins Toxicology |
| author_sort |
dietrich, richard |
| spelling |
Dietrich, Richard Schneider, Elisabeth Usleber, Ewald Märtibauer, Erwin 1056-9014 1522-7189 Wiley Toxicology http://dx.doi.org/10.1002/nt.2620030423 <jats:title>Abstract</jats:title><jats:p>Hybridomas producing monoclonal antibodies against aflatoxin M<jats:sub>1</jats:sub>, ochratoxin A, zearalenone, T‐2 toxin, diacetoxyscirpenol, 3‐acetyl‐deoxynivalenol, fusarenon X, and roridin A were developed after immunization of BALB/c mice and fusion of the splenocytes with myeloma cells. The antibodies were characterized in terms of immunoglobulin subclass, sensitivity, and specificity. The use of these antibodies in competitive enzyme immunoassays, either as microtiter plate assays or membrane‐based quick tests, as well as for the production of immunoaffinity columns is described. The advantages and disadvantages of monoclonal antibodies compared to polyclonal antisera for the improvement of mycotoxin analysis are discussed. © 1995 Wiley‐Liss, Inc.</jats:p> Use of monoclonal antibodies for the analysis of mycotoxins Natural Toxins |
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Natural Toxins |
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| title |
Use of monoclonal antibodies for the analysis of mycotoxins |
| title_unstemmed |
Use of monoclonal antibodies for the analysis of mycotoxins |
| title_full |
Use of monoclonal antibodies for the analysis of mycotoxins |
| title_fullStr |
Use of monoclonal antibodies for the analysis of mycotoxins |
| title_full_unstemmed |
Use of monoclonal antibodies for the analysis of mycotoxins |
| title_short |
Use of monoclonal antibodies for the analysis of mycotoxins |
| title_sort |
use of monoclonal antibodies for the analysis of mycotoxins |
| topic |
Toxicology |
| url |
http://dx.doi.org/10.1002/nt.2620030423 |
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1995 |
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288-293 |
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<jats:title>Abstract</jats:title><jats:p>Hybridomas producing monoclonal antibodies against aflatoxin M<jats:sub>1</jats:sub>, ochratoxin A, zearalenone, T‐2 toxin, diacetoxyscirpenol, 3‐acetyl‐deoxynivalenol, fusarenon X, and roridin A were developed after immunization of BALB/c mice and fusion of the splenocytes with myeloma cells. The antibodies were characterized in terms of immunoglobulin subclass, sensitivity, and specificity. The use of these antibodies in competitive enzyme immunoassays, either as microtiter plate assays or membrane‐based quick tests, as well as for the production of immunoaffinity columns is described. The advantages and disadvantages of monoclonal antibodies compared to polyclonal antisera for the improvement of mycotoxin analysis are discussed. © 1995 Wiley‐Liss, Inc.</jats:p> |
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| author | Dietrich, Richard, Schneider, Elisabeth, Usleber, Ewald, Märtibauer, Erwin |
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| description | <jats:title>Abstract</jats:title><jats:p>Hybridomas producing monoclonal antibodies against aflatoxin M<jats:sub>1</jats:sub>, ochratoxin A, zearalenone, T‐2 toxin, diacetoxyscirpenol, 3‐acetyl‐deoxynivalenol, fusarenon X, and roridin A were developed after immunization of BALB/c mice and fusion of the splenocytes with myeloma cells. The antibodies were characterized in terms of immunoglobulin subclass, sensitivity, and specificity. The use of these antibodies in competitive enzyme immunoassays, either as microtiter plate assays or membrane‐based quick tests, as well as for the production of immunoaffinity columns is described. The advantages and disadvantages of monoclonal antibodies compared to polyclonal antisera for the improvement of mycotoxin analysis are discussed. © 1995 Wiley‐Liss, Inc.</jats:p> |
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| imprint | Wiley, 1995 |
| imprint_str_mv | Wiley, 1995 |
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| physical | 288-293 |
| publishDate | 1995 |
| publishDateSort | 1995 |
| publisher | Wiley |
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| recordtype | ai |
| series | Natural Toxins |
| source_id | 49 |
| spelling | Dietrich, Richard Schneider, Elisabeth Usleber, Ewald Märtibauer, Erwin 1056-9014 1522-7189 Wiley Toxicology http://dx.doi.org/10.1002/nt.2620030423 <jats:title>Abstract</jats:title><jats:p>Hybridomas producing monoclonal antibodies against aflatoxin M<jats:sub>1</jats:sub>, ochratoxin A, zearalenone, T‐2 toxin, diacetoxyscirpenol, 3‐acetyl‐deoxynivalenol, fusarenon X, and roridin A were developed after immunization of BALB/c mice and fusion of the splenocytes with myeloma cells. The antibodies were characterized in terms of immunoglobulin subclass, sensitivity, and specificity. The use of these antibodies in competitive enzyme immunoassays, either as microtiter plate assays or membrane‐based quick tests, as well as for the production of immunoaffinity columns is described. The advantages and disadvantages of monoclonal antibodies compared to polyclonal antisera for the improvement of mycotoxin analysis are discussed. © 1995 Wiley‐Liss, Inc.</jats:p> Use of monoclonal antibodies for the analysis of mycotoxins Natural Toxins |
| spellingShingle | Dietrich, Richard, Schneider, Elisabeth, Usleber, Ewald, Märtibauer, Erwin, Natural Toxins, Use of monoclonal antibodies for the analysis of mycotoxins, Toxicology |
| title | Use of monoclonal antibodies for the analysis of mycotoxins |
| title_full | Use of monoclonal antibodies for the analysis of mycotoxins |
| title_fullStr | Use of monoclonal antibodies for the analysis of mycotoxins |
| title_full_unstemmed | Use of monoclonal antibodies for the analysis of mycotoxins |
| title_short | Use of monoclonal antibodies for the analysis of mycotoxins |
| title_sort | use of monoclonal antibodies for the analysis of mycotoxins |
| title_unstemmed | Use of monoclonal antibodies for the analysis of mycotoxins |
| topic | Toxicology |
| url | http://dx.doi.org/10.1002/nt.2620030423 |