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Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein
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| Zeitschriftentitel: | Molecular Nutrition & Food Research |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Molecular Nutrition & Food Research, 54, 2010, S1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Lei, Yen‐Ping Liu, Cheng‐Tzu Sheen, Lee‐Yan Chen, Haw‐Wen Lii, Chong‐Kuei Lei, Yen‐Ping Liu, Cheng‐Tzu Sheen, Lee‐Yan Chen, Haw‐Wen Lii, Chong‐Kuei |
|---|---|
| author |
Lei, Yen‐Ping Liu, Cheng‐Tzu Sheen, Lee‐Yan Chen, Haw‐Wen Lii, Chong‐Kuei |
| spellingShingle |
Lei, Yen‐Ping Liu, Cheng‐Tzu Sheen, Lee‐Yan Chen, Haw‐Wen Lii, Chong‐Kuei Molecular Nutrition & Food Research Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein Food Science Biotechnology |
| author_sort |
lei, yen‐ping |
| spelling |
Lei, Yen‐Ping Liu, Cheng‐Tzu Sheen, Lee‐Yan Chen, Haw‐Wen Lii, Chong‐Kuei 1613-4125 1613-4133 Wiley Food Science Biotechnology http://dx.doi.org/10.1002/mnfr.200900278 <jats:title>Abstract</jats:title><jats:p>Garlic is viewed as an effective health food against atherosclerosis. In this study, we examined whether diallyl disulfide (DADS) and diallyl trisulfide (DATS) protect endothelial nitric oxide synthase (eNOS) activation against oxidized LDL (ox‐LDL) insult and through what mechanism. We found that DADS and DATS reversed the suppression of eNOS Ser1177 phosphorylation by ox‐LDL, and wortmannin abolished the reversal by DADS and DATS. Similarly, the inhibition of cellular cGMP and nitric oxide production by ox‐LDL was reversed by DADS and DATS (<jats:italic>p</jats:italic><0.05). This increase in nitric oxide bioavailability by the allyl sulfides was attenuated by wortmannin. Immunoprecipitation assay revealed that DADS and DATS preserved the interaction of eNOS with caveolin‐1 in the membrane. In addition, DADS and DATS suppressed the reduction of the cellular eNOS protein content by ox‐LDL. When cycloheximide was added to block protein synthesis, DADS and DATS suppressed eNOS protein degradation similarly to that noted by MG132. Ox‐LDL increased chymotrypsin‐like proteasome activity, and this increase was inhibited by the allyl sulfides and MG132 (<jats:italic>p</jats:italic><0.05). These results suggest that DADS and DATS protect eNOS activity against ox‐LDL insult. This protection can be attributed partly to their mediation of phosphatidylinositol 3‐kinase/protein kinase B signaling and prevention of eNOS degradation.</jats:p> Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein Molecular Nutrition & Food Research |
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10.1002/mnfr.200900278 |
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| title |
Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| title_unstemmed |
Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| title_full |
Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| title_fullStr |
Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| title_full_unstemmed |
Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| title_short |
Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| title_sort |
diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| topic |
Food Science Biotechnology |
| url |
http://dx.doi.org/10.1002/mnfr.200900278 |
| publishDate |
2010 |
| physical |
|
| description |
<jats:title>Abstract</jats:title><jats:p>Garlic is viewed as an effective health food against atherosclerosis. In this study, we examined whether diallyl disulfide (DADS) and diallyl trisulfide (DATS) protect endothelial nitric oxide synthase (eNOS) activation against oxidized LDL (ox‐LDL) insult and through what mechanism. We found that DADS and DATS reversed the suppression of eNOS Ser1177 phosphorylation by ox‐LDL, and wortmannin abolished the reversal by DADS and DATS. Similarly, the inhibition of cellular cGMP and nitric oxide production by ox‐LDL was reversed by DADS and DATS (<jats:italic>p</jats:italic><0.05). This increase in nitric oxide bioavailability by the allyl sulfides was attenuated by wortmannin. Immunoprecipitation assay revealed that DADS and DATS preserved the interaction of eNOS with caveolin‐1 in the membrane. In addition, DADS and DATS suppressed the reduction of the cellular eNOS protein content by ox‐LDL. When cycloheximide was added to block protein synthesis, DADS and DATS suppressed eNOS protein degradation similarly to that noted by MG132. Ox‐LDL increased chymotrypsin‐like proteasome activity, and this increase was inhibited by the allyl sulfides and MG132 (<jats:italic>p</jats:italic><0.05). These results suggest that DADS and DATS protect eNOS activity against ox‐LDL insult. This protection can be attributed partly to their mediation of phosphatidylinositol 3‐kinase/protein kinase B signaling and prevention of eNOS degradation.</jats:p> |
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| author | Lei, Yen‐Ping, Liu, Cheng‐Tzu, Sheen, Lee‐Yan, Chen, Haw‐Wen, Lii, Chong‐Kuei |
| author_facet | Lei, Yen‐Ping, Liu, Cheng‐Tzu, Sheen, Lee‐Yan, Chen, Haw‐Wen, Lii, Chong‐Kuei, Lei, Yen‐Ping, Liu, Cheng‐Tzu, Sheen, Lee‐Yan, Chen, Haw‐Wen, Lii, Chong‐Kuei |
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| description | <jats:title>Abstract</jats:title><jats:p>Garlic is viewed as an effective health food against atherosclerosis. In this study, we examined whether diallyl disulfide (DADS) and diallyl trisulfide (DATS) protect endothelial nitric oxide synthase (eNOS) activation against oxidized LDL (ox‐LDL) insult and through what mechanism. We found that DADS and DATS reversed the suppression of eNOS Ser1177 phosphorylation by ox‐LDL, and wortmannin abolished the reversal by DADS and DATS. Similarly, the inhibition of cellular cGMP and nitric oxide production by ox‐LDL was reversed by DADS and DATS (<jats:italic>p</jats:italic><0.05). This increase in nitric oxide bioavailability by the allyl sulfides was attenuated by wortmannin. Immunoprecipitation assay revealed that DADS and DATS preserved the interaction of eNOS with caveolin‐1 in the membrane. In addition, DADS and DATS suppressed the reduction of the cellular eNOS protein content by ox‐LDL. When cycloheximide was added to block protein synthesis, DADS and DATS suppressed eNOS protein degradation similarly to that noted by MG132. Ox‐LDL increased chymotrypsin‐like proteasome activity, and this increase was inhibited by the allyl sulfides and MG132 (<jats:italic>p</jats:italic><0.05). These results suggest that DADS and DATS protect eNOS activity against ox‐LDL insult. This protection can be attributed partly to their mediation of phosphatidylinositol 3‐kinase/protein kinase B signaling and prevention of eNOS degradation.</jats:p> |
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| spelling | Lei, Yen‐Ping Liu, Cheng‐Tzu Sheen, Lee‐Yan Chen, Haw‐Wen Lii, Chong‐Kuei 1613-4125 1613-4133 Wiley Food Science Biotechnology http://dx.doi.org/10.1002/mnfr.200900278 <jats:title>Abstract</jats:title><jats:p>Garlic is viewed as an effective health food against atherosclerosis. In this study, we examined whether diallyl disulfide (DADS) and diallyl trisulfide (DATS) protect endothelial nitric oxide synthase (eNOS) activation against oxidized LDL (ox‐LDL) insult and through what mechanism. We found that DADS and DATS reversed the suppression of eNOS Ser1177 phosphorylation by ox‐LDL, and wortmannin abolished the reversal by DADS and DATS. Similarly, the inhibition of cellular cGMP and nitric oxide production by ox‐LDL was reversed by DADS and DATS (<jats:italic>p</jats:italic><0.05). This increase in nitric oxide bioavailability by the allyl sulfides was attenuated by wortmannin. Immunoprecipitation assay revealed that DADS and DATS preserved the interaction of eNOS with caveolin‐1 in the membrane. In addition, DADS and DATS suppressed the reduction of the cellular eNOS protein content by ox‐LDL. When cycloheximide was added to block protein synthesis, DADS and DATS suppressed eNOS protein degradation similarly to that noted by MG132. Ox‐LDL increased chymotrypsin‐like proteasome activity, and this increase was inhibited by the allyl sulfides and MG132 (<jats:italic>p</jats:italic><0.05). These results suggest that DADS and DATS protect eNOS activity against ox‐LDL insult. This protection can be attributed partly to their mediation of phosphatidylinositol 3‐kinase/protein kinase B signaling and prevention of eNOS degradation.</jats:p> Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein Molecular Nutrition & Food Research |
| spellingShingle | Lei, Yen‐Ping, Liu, Cheng‐Tzu, Sheen, Lee‐Yan, Chen, Haw‐Wen, Lii, Chong‐Kuei, Molecular Nutrition & Food Research, Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein, Food Science, Biotechnology |
| title | Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| title_full | Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| title_fullStr | Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| title_full_unstemmed | Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| title_short | Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| title_sort | diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| title_unstemmed | Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low‐density lipoprotein |
| topic | Food Science, Biotechnology |
| url | http://dx.doi.org/10.1002/mnfr.200900278 |