Eintrag weiter verarbeiten
Raman difference spectroscopy as a probe of biological molecules
Gespeichert in:
| Zeitschriftentitel: | Journal of Raman Spectroscopy |
|---|---|
| Personen und Körperschaften: | |
| In: | Journal of Raman Spectroscopy, 10, 1981, 1, S. 94-99 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Rousseau, D. L. Rousseau, D. L. |
|---|---|
| author |
Rousseau, D. L. |
| spellingShingle |
Rousseau, D. L. Journal of Raman Spectroscopy Raman difference spectroscopy as a probe of biological molecules Spectroscopy General Materials Science |
| author_sort |
rousseau, d. l. |
| spelling |
Rousseau, D. L. 0377-0486 1097-4555 Wiley Spectroscopy General Materials Science http://dx.doi.org/10.1002/jrs.1250100116 <jats:title>Abstract</jats:title><jats:p>The application of Raman difference spectroscopy to biological molecules, briefly reported previously,<jats:sup>1</jats:sup> is described here in detail. With Raman difference instrumentation frequency differences of less than 0.1 cm<jats:sup>−1</jats:sup> in 10 cm<jats:sup>−1</jats:sup> wide lines may be reliably detected. The limitation in the ultimate sensitivity for the detection of small differences lies in the signal to noise ratio, the presence of multiple lines and line shape differences. Spectrometer resetability, spectral resolution and step increment size impose no severe restrictions on obtaining small frequency differences. Differences in Raman excitation profiles between two samples may also be examined with this technique, and by making a simple adaptation Raman polarization difference spectra may be studied as well.</jats:p> Raman difference spectroscopy as a probe of biological molecules Journal of Raman Spectroscopy |
| doi_str_mv |
10.1002/jrs.1250100116 |
| facet_avail |
Online |
| finc_class_facet |
Physik |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9qcnMuMTI1MDEwMDExNg |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9qcnMuMTI1MDEwMDExNg |
| institution |
DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 |
| imprint |
Wiley, 1981 |
| imprint_str_mv |
Wiley, 1981 |
| issn |
0377-0486 1097-4555 |
| issn_str_mv |
0377-0486 1097-4555 |
| language |
English |
| mega_collection |
Wiley (CrossRef) |
| match_str |
rousseau1981ramandifferencespectroscopyasaprobeofbiologicalmolecules |
| publishDateSort |
1981 |
| publisher |
Wiley |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Raman Spectroscopy |
| source_id |
49 |
| title |
Raman difference spectroscopy as a probe of biological molecules |
| title_unstemmed |
Raman difference spectroscopy as a probe of biological molecules |
| title_full |
Raman difference spectroscopy as a probe of biological molecules |
| title_fullStr |
Raman difference spectroscopy as a probe of biological molecules |
| title_full_unstemmed |
Raman difference spectroscopy as a probe of biological molecules |
| title_short |
Raman difference spectroscopy as a probe of biological molecules |
| title_sort |
raman difference spectroscopy as a probe of biological molecules |
| topic |
Spectroscopy General Materials Science |
| url |
http://dx.doi.org/10.1002/jrs.1250100116 |
| publishDate |
1981 |
| physical |
94-99 |
| description |
<jats:title>Abstract</jats:title><jats:p>The application of Raman difference spectroscopy to biological molecules, briefly reported previously,<jats:sup>1</jats:sup> is described here in detail. With Raman difference instrumentation frequency differences of less than 0.1 cm<jats:sup>−1</jats:sup> in 10 cm<jats:sup>−1</jats:sup> wide lines may be reliably detected. The limitation in the ultimate sensitivity for the detection of small differences lies in the signal to noise ratio, the presence of multiple lines and line shape differences. Spectrometer resetability, spectral resolution and step increment size impose no severe restrictions on obtaining small frequency differences. Differences in Raman excitation profiles between two samples may also be examined with this technique, and by making a simple adaptation Raman polarization difference spectra may be studied as well.</jats:p> |
| container_issue |
1 |
| container_start_page |
94 |
| container_title |
Journal of Raman Spectroscopy |
| container_volume |
10 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792346467510255619 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:39:51.958Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Raman+difference+spectroscopy+as+a+probe+of+biological+molecules&rft.date=1981-01-01&genre=article&issn=1097-4555&volume=10&issue=1&spage=94&epage=99&pages=94-99&jtitle=Journal+of+Raman+Spectroscopy&atitle=Raman+difference+spectroscopy+as+a+probe+of+biological+molecules&aulast=Rousseau&aufirst=D.+L.&rft_id=info%3Adoi%2F10.1002%2Fjrs.1250100116&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792346467510255619 |
| author | Rousseau, D. L. |
| author_facet | Rousseau, D. L., Rousseau, D. L. |
| author_sort | rousseau, d. l. |
| container_issue | 1 |
| container_start_page | 94 |
| container_title | Journal of Raman Spectroscopy |
| container_volume | 10 |
| description | <jats:title>Abstract</jats:title><jats:p>The application of Raman difference spectroscopy to biological molecules, briefly reported previously,<jats:sup>1</jats:sup> is described here in detail. With Raman difference instrumentation frequency differences of less than 0.1 cm<jats:sup>−1</jats:sup> in 10 cm<jats:sup>−1</jats:sup> wide lines may be reliably detected. The limitation in the ultimate sensitivity for the detection of small differences lies in the signal to noise ratio, the presence of multiple lines and line shape differences. Spectrometer resetability, spectral resolution and step increment size impose no severe restrictions on obtaining small frequency differences. Differences in Raman excitation profiles between two samples may also be examined with this technique, and by making a simple adaptation Raman polarization difference spectra may be studied as well.</jats:p> |
| doi_str_mv | 10.1002/jrs.1250100116 |
| facet_avail | Online |
| finc_class_facet | Physik |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9qcnMuMTI1MDEwMDExNg |
| imprint | Wiley, 1981 |
| imprint_str_mv | Wiley, 1981 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14 |
| issn | 0377-0486, 1097-4555 |
| issn_str_mv | 0377-0486, 1097-4555 |
| language | English |
| last_indexed | 2024-03-01T17:39:51.958Z |
| match_str | rousseau1981ramandifferencespectroscopyasaprobeofbiologicalmolecules |
| mega_collection | Wiley (CrossRef) |
| physical | 94-99 |
| publishDate | 1981 |
| publishDateSort | 1981 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | Journal of Raman Spectroscopy |
| source_id | 49 |
| spelling | Rousseau, D. L. 0377-0486 1097-4555 Wiley Spectroscopy General Materials Science http://dx.doi.org/10.1002/jrs.1250100116 <jats:title>Abstract</jats:title><jats:p>The application of Raman difference spectroscopy to biological molecules, briefly reported previously,<jats:sup>1</jats:sup> is described here in detail. With Raman difference instrumentation frequency differences of less than 0.1 cm<jats:sup>−1</jats:sup> in 10 cm<jats:sup>−1</jats:sup> wide lines may be reliably detected. The limitation in the ultimate sensitivity for the detection of small differences lies in the signal to noise ratio, the presence of multiple lines and line shape differences. Spectrometer resetability, spectral resolution and step increment size impose no severe restrictions on obtaining small frequency differences. Differences in Raman excitation profiles between two samples may also be examined with this technique, and by making a simple adaptation Raman polarization difference spectra may be studied as well.</jats:p> Raman difference spectroscopy as a probe of biological molecules Journal of Raman Spectroscopy |
| spellingShingle | Rousseau, D. L., Journal of Raman Spectroscopy, Raman difference spectroscopy as a probe of biological molecules, Spectroscopy, General Materials Science |
| title | Raman difference spectroscopy as a probe of biological molecules |
| title_full | Raman difference spectroscopy as a probe of biological molecules |
| title_fullStr | Raman difference spectroscopy as a probe of biological molecules |
| title_full_unstemmed | Raman difference spectroscopy as a probe of biological molecules |
| title_short | Raman difference spectroscopy as a probe of biological molecules |
| title_sort | raman difference spectroscopy as a probe of biological molecules |
| title_unstemmed | Raman difference spectroscopy as a probe of biological molecules |
| topic | Spectroscopy, General Materials Science |
| url | http://dx.doi.org/10.1002/jrs.1250100116 |