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Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells
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| Zeitschriftentitel: | Journal of Neuroscience Research |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Journal of Neuroscience Research, 33, 1992, 2, S. 297-305 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Gubits, R. M. Yu, H. Casey, G. Munell, F. Vitek, M. P. Gubits, R. M. Yu, H. Casey, G. Munell, F. Vitek, M. P. |
|---|---|
| author |
Gubits, R. M. Yu, H. Casey, G. Munell, F. Vitek, M. P. |
| spellingShingle |
Gubits, R. M. Yu, H. Casey, G. Munell, F. Vitek, M. P. Journal of Neuroscience Research Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells Cellular and Molecular Neuroscience |
| author_sort |
gubits, r. m. |
| spelling |
Gubits, R. M. Yu, H. Casey, G. Munell, F. Vitek, M. P. 0360-4012 1097-4547 Wiley Cellular and Molecular Neuroscience http://dx.doi.org/10.1002/jnr.490330213 <jats:title>Abstract</jats:title><jats:p>Previous studies have demonstrated variability in the phenotype of rat C6 glioma cells. In the present study, we compared morphology, growth rate, and β‐adrenergic regulation of gene expression in early (P39–47) and late (P55–90) passage C6 cells. Morphological changes were observed in five independently derived, late passage populations. In four of the five, the untreated cells were more polygonal than the fibroblast‐like parental cells, and only a small fraction exhibited process outgrowth after dbcAMP treatment. Untreated cells from the fifth late passage population had longer cytoplasmic processes than parental cells and responded to dbcAMP with further process outgrowth. All late passage populations had shorter generation times than the parental cells. In early passage cells, treatment with the β‐adrenergic agonist, isoproterenol (IPR), resulted in an increase in c‐fos mRNA and a decrease in c‐jun mRNA (Gubits RM, Yu H: J Neurosci Res, 30:625–630, 1991). Both of these immediate early gene responses were irreversibly lost between P50 and P55. Additional differences in basal or IPR‐induced mRNA levels were observed for β‐APP, GFAP, NGF, and PPE, but not for a number of other mRNAs. These results are discussed in relationship to previously described differences in the ability of early and late passage C6 cells to accumulate cAMP (Mallorga P, et al.: Biochim Biophys Acta 678:221–229, 1981). © Wiley‐Liss, Inc.</jats:p> Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells Journal of Neuroscience Research |
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| title |
Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells |
| title_unstemmed |
Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells |
| title_full |
Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells |
| title_fullStr |
Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells |
| title_full_unstemmed |
Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells |
| title_short |
Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells |
| title_sort |
altered genetic response to β‐adrenergic receptor activation in late passage c6 glioma cells |
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Cellular and Molecular Neuroscience |
| url |
http://dx.doi.org/10.1002/jnr.490330213 |
| publishDate |
1992 |
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297-305 |
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<jats:title>Abstract</jats:title><jats:p>Previous studies have demonstrated variability in the phenotype of rat C6 glioma cells. In the present study, we compared morphology, growth rate, and β‐adrenergic regulation of gene expression in early (P39–47) and late (P55–90) passage C6 cells. Morphological changes were observed in five independently derived, late passage populations. In four of the five, the untreated cells were more polygonal than the fibroblast‐like parental cells, and only a small fraction exhibited process outgrowth after dbcAMP treatment. Untreated cells from the fifth late passage population had longer cytoplasmic processes than parental cells and responded to dbcAMP with further process outgrowth. All late passage populations had shorter generation times than the parental cells. In early passage cells, treatment with the β‐adrenergic agonist, isoproterenol (IPR), resulted in an increase in c‐fos mRNA and a decrease in c‐jun mRNA (Gubits RM, Yu H: J Neurosci Res, 30:625–630, 1991). Both of these immediate early gene responses were irreversibly lost between P50 and P55. Additional differences in basal or IPR‐induced mRNA levels were observed for β‐APP, GFAP, NGF, and PPE, but not for a number of other mRNAs. These results are discussed in relationship to previously described differences in the ability of early and late passage C6 cells to accumulate cAMP (Mallorga P, et al.: Biochim Biophys Acta 678:221–229, 1981). © Wiley‐Liss, Inc.</jats:p> |
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| author | Gubits, R. M., Yu, H., Casey, G., Munell, F., Vitek, M. P. |
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| description | <jats:title>Abstract</jats:title><jats:p>Previous studies have demonstrated variability in the phenotype of rat C6 glioma cells. In the present study, we compared morphology, growth rate, and β‐adrenergic regulation of gene expression in early (P39–47) and late (P55–90) passage C6 cells. Morphological changes were observed in five independently derived, late passage populations. In four of the five, the untreated cells were more polygonal than the fibroblast‐like parental cells, and only a small fraction exhibited process outgrowth after dbcAMP treatment. Untreated cells from the fifth late passage population had longer cytoplasmic processes than parental cells and responded to dbcAMP with further process outgrowth. All late passage populations had shorter generation times than the parental cells. In early passage cells, treatment with the β‐adrenergic agonist, isoproterenol (IPR), resulted in an increase in c‐fos mRNA and a decrease in c‐jun mRNA (Gubits RM, Yu H: J Neurosci Res, 30:625–630, 1991). Both of these immediate early gene responses were irreversibly lost between P50 and P55. Additional differences in basal or IPR‐induced mRNA levels were observed for β‐APP, GFAP, NGF, and PPE, but not for a number of other mRNAs. These results are discussed in relationship to previously described differences in the ability of early and late passage C6 cells to accumulate cAMP (Mallorga P, et al.: Biochim Biophys Acta 678:221–229, 1981). © Wiley‐Liss, Inc.</jats:p> |
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| spelling | Gubits, R. M. Yu, H. Casey, G. Munell, F. Vitek, M. P. 0360-4012 1097-4547 Wiley Cellular and Molecular Neuroscience http://dx.doi.org/10.1002/jnr.490330213 <jats:title>Abstract</jats:title><jats:p>Previous studies have demonstrated variability in the phenotype of rat C6 glioma cells. In the present study, we compared morphology, growth rate, and β‐adrenergic regulation of gene expression in early (P39–47) and late (P55–90) passage C6 cells. Morphological changes were observed in five independently derived, late passage populations. In four of the five, the untreated cells were more polygonal than the fibroblast‐like parental cells, and only a small fraction exhibited process outgrowth after dbcAMP treatment. Untreated cells from the fifth late passage population had longer cytoplasmic processes than parental cells and responded to dbcAMP with further process outgrowth. All late passage populations had shorter generation times than the parental cells. In early passage cells, treatment with the β‐adrenergic agonist, isoproterenol (IPR), resulted in an increase in c‐fos mRNA and a decrease in c‐jun mRNA (Gubits RM, Yu H: J Neurosci Res, 30:625–630, 1991). Both of these immediate early gene responses were irreversibly lost between P50 and P55. Additional differences in basal or IPR‐induced mRNA levels were observed for β‐APP, GFAP, NGF, and PPE, but not for a number of other mRNAs. These results are discussed in relationship to previously described differences in the ability of early and late passage C6 cells to accumulate cAMP (Mallorga P, et al.: Biochim Biophys Acta 678:221–229, 1981). © Wiley‐Liss, Inc.</jats:p> Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells Journal of Neuroscience Research |
| spellingShingle | Gubits, R. M., Yu, H., Casey, G., Munell, F., Vitek, M. P., Journal of Neuroscience Research, Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells, Cellular and Molecular Neuroscience |
| title | Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells |
| title_full | Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells |
| title_fullStr | Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells |
| title_full_unstemmed | Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells |
| title_short | Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells |
| title_sort | altered genetic response to β‐adrenergic receptor activation in late passage c6 glioma cells |
| title_unstemmed | Altered genetic response to β‐adrenergic receptor activation in late passage C6 glioma cells |
| topic | Cellular and Molecular Neuroscience |
| url | http://dx.doi.org/10.1002/jnr.490330213 |