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In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration
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| Zeitschriftentitel: | Journal of Biomedical Materials Research Part A |
|---|---|
| Personen und Körperschaften: | , |
| In: | Journal of Biomedical Materials Research Part A, 104, 2016, 10, S. 2383-2393 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Pehlivaner Kara, Meryem O. Ekenseair, Adam K. Pehlivaner Kara, Meryem O. Ekenseair, Adam K. |
|---|---|
| author |
Pehlivaner Kara, Meryem O. Ekenseair, Adam K. |
| spellingShingle |
Pehlivaner Kara, Meryem O. Ekenseair, Adam K. Journal of Biomedical Materials Research Part A In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration Metals and Alloys Biomedical Engineering Biomaterials Ceramics and Composites |
| author_sort |
pehlivaner kara, meryem o. |
| spelling |
Pehlivaner Kara, Meryem O. Ekenseair, Adam K. 1549-3296 1552-4965 Wiley Metals and Alloys Biomedical Engineering Biomaterials Ceramics and Composites http://dx.doi.org/10.1002/jbm.a.35774 <jats:title>Abstract</jats:title><jats:p>In this study, the efficacy of creating cellular hydrogel coatings on warm tissue surfaces through the minimally invasive, sprayable delivery of thermoresponsive liquid solutions was investigated. Poly(<jats:italic>N</jats:italic>‐isopropylacrylamide)‐based (pNiPAAm) thermogelling macromers with or without addition of crosslinking polyamidoamine (PAMAM) macromers were synthesized and used to produce <jats:italic>in situ</jats:italic> forming thermally and chemically gelling hydrogel systems. The effect of solution and process parameters on hydrogel physical properties and morphology was evaluated and compared to poly(ethylene glycol) and injection controls. Smooth, fast, and conformal hydrogel coatings were obtained when pNiPAAm thermogelling macromers were sprayed with high PAMAM concentration at low pressure. Cellular hydrogel coatings were further fabricated by different spraying techniques: single‐stream, layer‐by‐layer, and dual stream methods. The impact of spray technique, solution formulation, pressure, and spray solution viscosity on the viability of fibroblast and osteoblast cells encapsulated in hydrogels was elucidated. In particular, the early formation of chemically crosslinked micronetworks during bulk liquid flow was shown to significantly affect cell viability under turbulent conditions compared to injectable controls. The results demonstrated that sprayable, <jats:italic>in situ</jats:italic> forming hydrogels capable of delivering cell populations in a homogeneous therapeutic coating on diseased tissue surfaces offer promise as novel therapies for applications in regenerative medicine. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2383–2393, 2016.</jats:p> <i>In situ</i> spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration Journal of Biomedical Materials Research Part A |
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Journal of Biomedical Materials Research Part A |
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| title |
In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| title_unstemmed |
In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| title_full |
In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| title_fullStr |
In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| title_full_unstemmed |
In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| title_short |
In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| title_sort |
<i>in situ</i> spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| topic |
Metals and Alloys Biomedical Engineering Biomaterials Ceramics and Composites |
| url |
http://dx.doi.org/10.1002/jbm.a.35774 |
| publishDate |
2016 |
| physical |
2383-2393 |
| description |
<jats:title>Abstract</jats:title><jats:p>In this study, the efficacy of creating cellular hydrogel coatings on warm tissue surfaces through the minimally invasive, sprayable delivery of thermoresponsive liquid solutions was investigated. Poly(<jats:italic>N</jats:italic>‐isopropylacrylamide)‐based (pNiPAAm) thermogelling macromers with or without addition of crosslinking polyamidoamine (PAMAM) macromers were synthesized and used to produce <jats:italic>in situ</jats:italic> forming thermally and chemically gelling hydrogel systems. The effect of solution and process parameters on hydrogel physical properties and morphology was evaluated and compared to poly(ethylene glycol) and injection controls. Smooth, fast, and conformal hydrogel coatings were obtained when pNiPAAm thermogelling macromers were sprayed with high PAMAM concentration at low pressure. Cellular hydrogel coatings were further fabricated by different spraying techniques: single‐stream, layer‐by‐layer, and dual stream methods. The impact of spray technique, solution formulation, pressure, and spray solution viscosity on the viability of fibroblast and osteoblast cells encapsulated in hydrogels was elucidated. In particular, the early formation of chemically crosslinked micronetworks during bulk liquid flow was shown to significantly affect cell viability under turbulent conditions compared to injectable controls. The results demonstrated that sprayable, <jats:italic>in situ</jats:italic> forming hydrogels capable of delivering cell populations in a homogeneous therapeutic coating on diseased tissue surfaces offer promise as novel therapies for applications in regenerative medicine. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2383–2393, 2016.</jats:p> |
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| author | Pehlivaner Kara, Meryem O., Ekenseair, Adam K. |
| author_facet | Pehlivaner Kara, Meryem O., Ekenseair, Adam K., Pehlivaner Kara, Meryem O., Ekenseair, Adam K. |
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| description | <jats:title>Abstract</jats:title><jats:p>In this study, the efficacy of creating cellular hydrogel coatings on warm tissue surfaces through the minimally invasive, sprayable delivery of thermoresponsive liquid solutions was investigated. Poly(<jats:italic>N</jats:italic>‐isopropylacrylamide)‐based (pNiPAAm) thermogelling macromers with or without addition of crosslinking polyamidoamine (PAMAM) macromers were synthesized and used to produce <jats:italic>in situ</jats:italic> forming thermally and chemically gelling hydrogel systems. The effect of solution and process parameters on hydrogel physical properties and morphology was evaluated and compared to poly(ethylene glycol) and injection controls. Smooth, fast, and conformal hydrogel coatings were obtained when pNiPAAm thermogelling macromers were sprayed with high PAMAM concentration at low pressure. Cellular hydrogel coatings were further fabricated by different spraying techniques: single‐stream, layer‐by‐layer, and dual stream methods. The impact of spray technique, solution formulation, pressure, and spray solution viscosity on the viability of fibroblast and osteoblast cells encapsulated in hydrogels was elucidated. In particular, the early formation of chemically crosslinked micronetworks during bulk liquid flow was shown to significantly affect cell viability under turbulent conditions compared to injectable controls. The results demonstrated that sprayable, <jats:italic>in situ</jats:italic> forming hydrogels capable of delivering cell populations in a homogeneous therapeutic coating on diseased tissue surfaces offer promise as novel therapies for applications in regenerative medicine. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2383–2393, 2016.</jats:p> |
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| spelling | Pehlivaner Kara, Meryem O. Ekenseair, Adam K. 1549-3296 1552-4965 Wiley Metals and Alloys Biomedical Engineering Biomaterials Ceramics and Composites http://dx.doi.org/10.1002/jbm.a.35774 <jats:title>Abstract</jats:title><jats:p>In this study, the efficacy of creating cellular hydrogel coatings on warm tissue surfaces through the minimally invasive, sprayable delivery of thermoresponsive liquid solutions was investigated. Poly(<jats:italic>N</jats:italic>‐isopropylacrylamide)‐based (pNiPAAm) thermogelling macromers with or without addition of crosslinking polyamidoamine (PAMAM) macromers were synthesized and used to produce <jats:italic>in situ</jats:italic> forming thermally and chemically gelling hydrogel systems. The effect of solution and process parameters on hydrogel physical properties and morphology was evaluated and compared to poly(ethylene glycol) and injection controls. Smooth, fast, and conformal hydrogel coatings were obtained when pNiPAAm thermogelling macromers were sprayed with high PAMAM concentration at low pressure. Cellular hydrogel coatings were further fabricated by different spraying techniques: single‐stream, layer‐by‐layer, and dual stream methods. The impact of spray technique, solution formulation, pressure, and spray solution viscosity on the viability of fibroblast and osteoblast cells encapsulated in hydrogels was elucidated. In particular, the early formation of chemically crosslinked micronetworks during bulk liquid flow was shown to significantly affect cell viability under turbulent conditions compared to injectable controls. The results demonstrated that sprayable, <jats:italic>in situ</jats:italic> forming hydrogels capable of delivering cell populations in a homogeneous therapeutic coating on diseased tissue surfaces offer promise as novel therapies for applications in regenerative medicine. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2383–2393, 2016.</jats:p> <i>In situ</i> spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration Journal of Biomedical Materials Research Part A |
| spellingShingle | Pehlivaner Kara, Meryem O., Ekenseair, Adam K., Journal of Biomedical Materials Research Part A, In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration, Metals and Alloys, Biomedical Engineering, Biomaterials, Ceramics and Composites |
| title | In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| title_full | In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| title_fullStr | In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| title_full_unstemmed | In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| title_short | In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| title_sort | <i>in situ</i> spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| title_unstemmed | In situ spray deposition of cell‐loaded, thermally and chemically gelling hydrogel coatings for tissue regeneration |
| topic | Metals and Alloys, Biomedical Engineering, Biomaterials, Ceramics and Composites |
| url | http://dx.doi.org/10.1002/jbm.a.35774 |