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Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box
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| Zeitschriftentitel: | Journal of Applied Toxicology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Journal of Applied Toxicology, 38, 2018, 4, S. 437-449 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Otarola, Gaston Castillo, Hector Marcellini, Sylvain Otarola, Gaston Castillo, Hector Marcellini, Sylvain |
|---|---|
| author |
Otarola, Gaston Castillo, Hector Marcellini, Sylvain |
| spellingShingle |
Otarola, Gaston Castillo, Hector Marcellini, Sylvain Journal of Applied Toxicology Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box Toxicology |
| author_sort |
otarola, gaston |
| spelling |
Otarola, Gaston Castillo, Hector Marcellini, Sylvain 0260-437X 1099-1263 Wiley Toxicology http://dx.doi.org/10.1002/jat.3575 <jats:title>Abstract</jats:title><jats:p>Despite intensive media coverage and international regulations, man‐made persistent organic pollutants such as dioxins represent a serious environmental and health threat. Their detection by sophisticated chromatography technologies is highly complex, impeding the constant monitoring of food or environmental samples. This limitation has fostered the development of generations of bioassays exploiting the molecular function of the aryl hydrocarbon receptor (AhR), which binds toxic compounds and directly activates the transcription of target genes. Here, we review the rich panel of available AhR‐dependent bioassays and propose a novel classification based on the source of AhR, which can either be endogenously produced by cell types or tissues naturally responsive to dioxins, or exogenously introduced into a wide range of cellular contexts. In both cases, in vitro and in vivo strategies have been engineered to monitor the formation of molecular complexes, and the activation of direct downstream targets or reporter genes. We evaluate and compare bioassays based on exogenous and endogenous AhR proteins and discuss their specific challenges, strengths and opportunities for futures applications. Undoubtedly, the dynamic field of AhR‐dependent bioassays will keep providing new and original strategies to help protect human health and ecosystems from persistent organic pollutants.</jats:p> Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box Journal of Applied Toxicology |
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| title |
Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box |
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Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box |
| title_full |
Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box |
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Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box |
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Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box |
| title_short |
Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box |
| title_sort |
aryl hydrocarbon receptor‐based bioassays for dioxin detection: thinking outside the box |
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Toxicology |
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http://dx.doi.org/10.1002/jat.3575 |
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2018 |
| physical |
437-449 |
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<jats:title>Abstract</jats:title><jats:p>Despite intensive media coverage and international regulations, man‐made persistent organic pollutants such as dioxins represent a serious environmental and health threat. Their detection by sophisticated chromatography technologies is highly complex, impeding the constant monitoring of food or environmental samples. This limitation has fostered the development of generations of bioassays exploiting the molecular function of the aryl hydrocarbon receptor (AhR), which binds toxic compounds and directly activates the transcription of target genes. Here, we review the rich panel of available AhR‐dependent bioassays and propose a novel classification based on the source of AhR, which can either be endogenously produced by cell types or tissues naturally responsive to dioxins, or exogenously introduced into a wide range of cellular contexts. In both cases, in vitro and in vivo strategies have been engineered to monitor the formation of molecular complexes, and the activation of direct downstream targets or reporter genes. We evaluate and compare bioassays based on exogenous and endogenous AhR proteins and discuss their specific challenges, strengths and opportunities for futures applications. Undoubtedly, the dynamic field of AhR‐dependent bioassays will keep providing new and original strategies to help protect human health and ecosystems from persistent organic pollutants.</jats:p> |
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| author | Otarola, Gaston, Castillo, Hector, Marcellini, Sylvain |
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| description | <jats:title>Abstract</jats:title><jats:p>Despite intensive media coverage and international regulations, man‐made persistent organic pollutants such as dioxins represent a serious environmental and health threat. Their detection by sophisticated chromatography technologies is highly complex, impeding the constant monitoring of food or environmental samples. This limitation has fostered the development of generations of bioassays exploiting the molecular function of the aryl hydrocarbon receptor (AhR), which binds toxic compounds and directly activates the transcription of target genes. Here, we review the rich panel of available AhR‐dependent bioassays and propose a novel classification based on the source of AhR, which can either be endogenously produced by cell types or tissues naturally responsive to dioxins, or exogenously introduced into a wide range of cellular contexts. In both cases, in vitro and in vivo strategies have been engineered to monitor the formation of molecular complexes, and the activation of direct downstream targets or reporter genes. We evaluate and compare bioassays based on exogenous and endogenous AhR proteins and discuss their specific challenges, strengths and opportunities for futures applications. Undoubtedly, the dynamic field of AhR‐dependent bioassays will keep providing new and original strategies to help protect human health and ecosystems from persistent organic pollutants.</jats:p> |
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| spelling | Otarola, Gaston Castillo, Hector Marcellini, Sylvain 0260-437X 1099-1263 Wiley Toxicology http://dx.doi.org/10.1002/jat.3575 <jats:title>Abstract</jats:title><jats:p>Despite intensive media coverage and international regulations, man‐made persistent organic pollutants such as dioxins represent a serious environmental and health threat. Their detection by sophisticated chromatography technologies is highly complex, impeding the constant monitoring of food or environmental samples. This limitation has fostered the development of generations of bioassays exploiting the molecular function of the aryl hydrocarbon receptor (AhR), which binds toxic compounds and directly activates the transcription of target genes. Here, we review the rich panel of available AhR‐dependent bioassays and propose a novel classification based on the source of AhR, which can either be endogenously produced by cell types or tissues naturally responsive to dioxins, or exogenously introduced into a wide range of cellular contexts. In both cases, in vitro and in vivo strategies have been engineered to monitor the formation of molecular complexes, and the activation of direct downstream targets or reporter genes. We evaluate and compare bioassays based on exogenous and endogenous AhR proteins and discuss their specific challenges, strengths and opportunities for futures applications. Undoubtedly, the dynamic field of AhR‐dependent bioassays will keep providing new and original strategies to help protect human health and ecosystems from persistent organic pollutants.</jats:p> Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box Journal of Applied Toxicology |
| spellingShingle | Otarola, Gaston, Castillo, Hector, Marcellini, Sylvain, Journal of Applied Toxicology, Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box, Toxicology |
| title | Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box |
| title_full | Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box |
| title_fullStr | Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box |
| title_full_unstemmed | Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box |
| title_short | Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box |
| title_sort | aryl hydrocarbon receptor‐based bioassays for dioxin detection: thinking outside the box |
| title_unstemmed | Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box |
| topic | Toxicology |
| url | http://dx.doi.org/10.1002/jat.3575 |