Eintrag weiter verarbeiten
Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia
Gespeichert in:
Zeitschriftentitel: | Journal of Cachexia, Sarcopenia and Muscle |
---|---|
Personen und Körperschaften: | , , , , , , |
In: | Journal of Cachexia, Sarcopenia and Muscle, 8, 2017, 3, S. 405-416 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Wiley
|
Schlagwörter: |
author_facet |
Narasimhan, Ashok Ghosh, Sunita Stretch, Cynthia Greiner, Russell Bathe, Oliver F. Baracos, Vickie Damaraju, Sambasivarao Narasimhan, Ashok Ghosh, Sunita Stretch, Cynthia Greiner, Russell Bathe, Oliver F. Baracos, Vickie Damaraju, Sambasivarao |
---|---|
author |
Narasimhan, Ashok Ghosh, Sunita Stretch, Cynthia Greiner, Russell Bathe, Oliver F. Baracos, Vickie Damaraju, Sambasivarao |
spellingShingle |
Narasimhan, Ashok Ghosh, Sunita Stretch, Cynthia Greiner, Russell Bathe, Oliver F. Baracos, Vickie Damaraju, Sambasivarao Journal of Cachexia, Sarcopenia and Muscle Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia Physiology (medical) Orthopedics and Sports Medicine |
author_sort |
narasimhan, ashok |
spelling |
Narasimhan, Ashok Ghosh, Sunita Stretch, Cynthia Greiner, Russell Bathe, Oliver F. Baracos, Vickie Damaraju, Sambasivarao 2190-5991 2190-6009 Wiley Physiology (medical) Orthopedics and Sports Medicine http://dx.doi.org/10.1002/jcsm.12168 <jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>MicroRNAs (miRs) are small non‐coding RNAs that regulate gene (mRNA) expression. Although the pathological role of miRs have been studied in muscle wasting conditions such as myotonic and muscular dystrophy, their roles in cancer cachexia (CC) are still emerging.</jats:p></jats:sec><jats:sec><jats:title>Objectives</jats:title><jats:p>The objectives are (i) to profile human skeletal muscle expressed miRs; (ii) to identify differentially expressed (DE) miRs between cachectic and non‐cachectic cancer patients; (iii) to identify mRNA targets for the DE miRs to gain mechanistic insights; and (iv) to investigate if miRs show potential prognostic and predictive value.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Study subjects were classified based on the international consensus diagnostic criteria for CC. Forty‐two cancer patients were included, of which 22 were cachectic cases and 20 were non‐cachectic cancer controls. Total RNA isolated from muscle biopsies were subjected to next‐generation sequencing.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>A total of 777 miRs were profiled, and 82 miRs with read counts of ≥5 in 80% of samples were retained for analysis. We identified eight DE miRs (up‐regulated, fold change of ≥1.4 at <jats:italic>P</jats:italic> < 0.05). A total of 191 potential mRNA targets were identified for the DE miRs using previously described human skeletal muscle mRNA expression data (<jats:italic>n</jats:italic> = 90), and a majority of them were also confirmed in an independent mRNA transcriptome dataset. Ingenuity pathway analysis identified pathways related to myogenesis and inflammation. qRT‐PCR analysis of representative miRs showed similar direction of effect (<jats:italic>P</jats:italic> < 0.05), as observed in next‐generation sequencing. The identified miRs also showed prognostic and predictive value.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>In all, we identified eight novel miRs associated with CC.</jats:p></jats:sec> Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia Journal of Cachexia, Sarcopenia and Muscle |
doi_str_mv |
10.1002/jcsm.12168 |
facet_avail |
Online Free |
finc_class_facet |
Biologie Medizin |
format |
ElectronicArticle |
fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9qY3NtLjEyMTY4 |
id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9qY3NtLjEyMTY4 |
institution |
DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Zwi2 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 |
imprint |
Wiley, 2017 |
imprint_str_mv |
Wiley, 2017 |
issn |
2190-5991 2190-6009 |
issn_str_mv |
2190-5991 2190-6009 |
language |
English |
mega_collection |
Wiley (CrossRef) |
match_str |
narasimhan2017smallrnaomeprofilingfromhumanskeletalmusclenovelmirnasandtheirtargetsassociatedwithcancercachexia |
publishDateSort |
2017 |
publisher |
Wiley |
recordtype |
ai |
record_format |
ai |
series |
Journal of Cachexia, Sarcopenia and Muscle |
source_id |
49 |
title |
Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia |
title_unstemmed |
Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia |
title_full |
Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia |
title_fullStr |
Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia |
title_full_unstemmed |
Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia |
title_short |
Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia |
title_sort |
small rnaome profiling from human skeletal muscle: novel mirnas and their targets associated with cancer cachexia |
topic |
Physiology (medical) Orthopedics and Sports Medicine |
url |
http://dx.doi.org/10.1002/jcsm.12168 |
publishDate |
2017 |
physical |
405-416 |
description |
<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>MicroRNAs (miRs) are small non‐coding RNAs that regulate gene (mRNA) expression. Although the pathological role of miRs have been studied in muscle wasting conditions such as myotonic and muscular dystrophy, their roles in cancer cachexia (CC) are still emerging.</jats:p></jats:sec><jats:sec><jats:title>Objectives</jats:title><jats:p>The objectives are (i) to profile human skeletal muscle expressed miRs; (ii) to identify differentially expressed (DE) miRs between cachectic and non‐cachectic cancer patients; (iii) to identify mRNA targets for the DE miRs to gain mechanistic insights; and (iv) to investigate if miRs show potential prognostic and predictive value.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Study subjects were classified based on the international consensus diagnostic criteria for CC. Forty‐two cancer patients were included, of which 22 were cachectic cases and 20 were non‐cachectic cancer controls. Total RNA isolated from muscle biopsies were subjected to next‐generation sequencing.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>A total of 777 miRs were profiled, and 82 miRs with read counts of ≥5 in 80% of samples were retained for analysis. We identified eight DE miRs (up‐regulated, fold change of ≥1.4 at <jats:italic>P</jats:italic> < 0.05). A total of 191 potential mRNA targets were identified for the DE miRs using previously described human skeletal muscle mRNA expression data (<jats:italic>n</jats:italic> = 90), and a majority of them were also confirmed in an independent mRNA transcriptome dataset. Ingenuity pathway analysis identified pathways related to myogenesis and inflammation. qRT‐PCR analysis of representative miRs showed similar direction of effect (<jats:italic>P</jats:italic> < 0.05), as observed in next‐generation sequencing. The identified miRs also showed prognostic and predictive value.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>In all, we identified eight novel miRs associated with CC.</jats:p></jats:sec> |
container_issue |
3 |
container_start_page |
405 |
container_title |
Journal of Cachexia, Sarcopenia and Muscle |
container_volume |
8 |
format_de105 |
Article, E-Article |
format_de14 |
Article, E-Article |
format_de15 |
Article, E-Article |
format_de520 |
Article, E-Article |
format_de540 |
Article, E-Article |
format_dech1 |
Article, E-Article |
format_ded117 |
Article, E-Article |
format_degla1 |
E-Article |
format_del152 |
Buch |
format_del189 |
Article, E-Article |
format_dezi4 |
Article |
format_dezwi2 |
Article, E-Article |
format_finc |
Article, E-Article |
format_nrw |
Article, E-Article |
_version_ |
1792348643044360193 |
geogr_code |
not assigned |
last_indexed |
2024-03-01T18:13:41.173Z |
geogr_code_person |
not assigned |
openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Small+RNAome+profiling+from+human+skeletal+muscle%3A+novel+miRNAs+and+their+targets+associated+with+cancer+cachexia&rft.date=2017-06-01&genre=article&issn=2190-6009&volume=8&issue=3&spage=405&epage=416&pages=405-416&jtitle=Journal+of+Cachexia%2C+Sarcopenia+and+Muscle&atitle=Small+RNAome+profiling+from+human+skeletal+muscle%3A+novel+miRNAs+and+their+targets+associated+with+cancer+cachexia&aulast=Damaraju&aufirst=Sambasivarao&rft_id=info%3Adoi%2F10.1002%2Fjcsm.12168&rft.language%5B0%5D=eng |
SOLR | |
_version_ | 1792348643044360193 |
author | Narasimhan, Ashok, Ghosh, Sunita, Stretch, Cynthia, Greiner, Russell, Bathe, Oliver F., Baracos, Vickie, Damaraju, Sambasivarao |
author_facet | Narasimhan, Ashok, Ghosh, Sunita, Stretch, Cynthia, Greiner, Russell, Bathe, Oliver F., Baracos, Vickie, Damaraju, Sambasivarao, Narasimhan, Ashok, Ghosh, Sunita, Stretch, Cynthia, Greiner, Russell, Bathe, Oliver F., Baracos, Vickie, Damaraju, Sambasivarao |
author_sort | narasimhan, ashok |
container_issue | 3 |
container_start_page | 405 |
container_title | Journal of Cachexia, Sarcopenia and Muscle |
container_volume | 8 |
description | <jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>MicroRNAs (miRs) are small non‐coding RNAs that regulate gene (mRNA) expression. Although the pathological role of miRs have been studied in muscle wasting conditions such as myotonic and muscular dystrophy, their roles in cancer cachexia (CC) are still emerging.</jats:p></jats:sec><jats:sec><jats:title>Objectives</jats:title><jats:p>The objectives are (i) to profile human skeletal muscle expressed miRs; (ii) to identify differentially expressed (DE) miRs between cachectic and non‐cachectic cancer patients; (iii) to identify mRNA targets for the DE miRs to gain mechanistic insights; and (iv) to investigate if miRs show potential prognostic and predictive value.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Study subjects were classified based on the international consensus diagnostic criteria for CC. Forty‐two cancer patients were included, of which 22 were cachectic cases and 20 were non‐cachectic cancer controls. Total RNA isolated from muscle biopsies were subjected to next‐generation sequencing.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>A total of 777 miRs were profiled, and 82 miRs with read counts of ≥5 in 80% of samples were retained for analysis. We identified eight DE miRs (up‐regulated, fold change of ≥1.4 at <jats:italic>P</jats:italic> < 0.05). A total of 191 potential mRNA targets were identified for the DE miRs using previously described human skeletal muscle mRNA expression data (<jats:italic>n</jats:italic> = 90), and a majority of them were also confirmed in an independent mRNA transcriptome dataset. Ingenuity pathway analysis identified pathways related to myogenesis and inflammation. qRT‐PCR analysis of representative miRs showed similar direction of effect (<jats:italic>P</jats:italic> < 0.05), as observed in next‐generation sequencing. The identified miRs also showed prognostic and predictive value.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>In all, we identified eight novel miRs associated with CC.</jats:p></jats:sec> |
doi_str_mv | 10.1002/jcsm.12168 |
facet_avail | Online, Free |
finc_class_facet | Biologie, Medizin |
format | ElectronicArticle |
format_de105 | Article, E-Article |
format_de14 | Article, E-Article |
format_de15 | Article, E-Article |
format_de520 | Article, E-Article |
format_de540 | Article, E-Article |
format_dech1 | Article, E-Article |
format_ded117 | Article, E-Article |
format_degla1 | E-Article |
format_del152 | Buch |
format_del189 | Article, E-Article |
format_dezi4 | Article |
format_dezwi2 | Article, E-Article |
format_finc | Article, E-Article |
format_nrw | Article, E-Article |
geogr_code | not assigned |
geogr_code_person | not assigned |
id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9qY3NtLjEyMTY4 |
imprint | Wiley, 2017 |
imprint_str_mv | Wiley, 2017 |
institution | DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
issn | 2190-5991, 2190-6009 |
issn_str_mv | 2190-5991, 2190-6009 |
language | English |
last_indexed | 2024-03-01T18:13:41.173Z |
match_str | narasimhan2017smallrnaomeprofilingfromhumanskeletalmusclenovelmirnasandtheirtargetsassociatedwithcancercachexia |
mega_collection | Wiley (CrossRef) |
physical | 405-416 |
publishDate | 2017 |
publishDateSort | 2017 |
publisher | Wiley |
record_format | ai |
recordtype | ai |
series | Journal of Cachexia, Sarcopenia and Muscle |
source_id | 49 |
spelling | Narasimhan, Ashok Ghosh, Sunita Stretch, Cynthia Greiner, Russell Bathe, Oliver F. Baracos, Vickie Damaraju, Sambasivarao 2190-5991 2190-6009 Wiley Physiology (medical) Orthopedics and Sports Medicine http://dx.doi.org/10.1002/jcsm.12168 <jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>MicroRNAs (miRs) are small non‐coding RNAs that regulate gene (mRNA) expression. Although the pathological role of miRs have been studied in muscle wasting conditions such as myotonic and muscular dystrophy, their roles in cancer cachexia (CC) are still emerging.</jats:p></jats:sec><jats:sec><jats:title>Objectives</jats:title><jats:p>The objectives are (i) to profile human skeletal muscle expressed miRs; (ii) to identify differentially expressed (DE) miRs between cachectic and non‐cachectic cancer patients; (iii) to identify mRNA targets for the DE miRs to gain mechanistic insights; and (iv) to investigate if miRs show potential prognostic and predictive value.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Study subjects were classified based on the international consensus diagnostic criteria for CC. Forty‐two cancer patients were included, of which 22 were cachectic cases and 20 were non‐cachectic cancer controls. Total RNA isolated from muscle biopsies were subjected to next‐generation sequencing.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>A total of 777 miRs were profiled, and 82 miRs with read counts of ≥5 in 80% of samples were retained for analysis. We identified eight DE miRs (up‐regulated, fold change of ≥1.4 at <jats:italic>P</jats:italic> < 0.05). A total of 191 potential mRNA targets were identified for the DE miRs using previously described human skeletal muscle mRNA expression data (<jats:italic>n</jats:italic> = 90), and a majority of them were also confirmed in an independent mRNA transcriptome dataset. Ingenuity pathway analysis identified pathways related to myogenesis and inflammation. qRT‐PCR analysis of representative miRs showed similar direction of effect (<jats:italic>P</jats:italic> < 0.05), as observed in next‐generation sequencing. The identified miRs also showed prognostic and predictive value.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>In all, we identified eight novel miRs associated with CC.</jats:p></jats:sec> Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia Journal of Cachexia, Sarcopenia and Muscle |
spellingShingle | Narasimhan, Ashok, Ghosh, Sunita, Stretch, Cynthia, Greiner, Russell, Bathe, Oliver F., Baracos, Vickie, Damaraju, Sambasivarao, Journal of Cachexia, Sarcopenia and Muscle, Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia, Physiology (medical), Orthopedics and Sports Medicine |
title | Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia |
title_full | Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia |
title_fullStr | Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia |
title_full_unstemmed | Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia |
title_short | Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia |
title_sort | small rnaome profiling from human skeletal muscle: novel mirnas and their targets associated with cancer cachexia |
title_unstemmed | Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia |
topic | Physiology (medical), Orthopedics and Sports Medicine |
url | http://dx.doi.org/10.1002/jcsm.12168 |