Eintrag weiter verarbeiten
MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells
Gespeichert in:
| Zeitschriftentitel: | Journal of Cellular Biochemistry |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Journal of Cellular Biochemistry, 121, 2020, 2, S. 1216-1226 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Kou, Jianqiang Zheng, Xiujun Guo, Jianwei Liu, Yang Liu, Xiangyun Kou, Jianqiang Zheng, Xiujun Guo, Jianwei Liu, Yang Liu, Xiangyun |
|---|---|
| author |
Kou, Jianqiang Zheng, Xiujun Guo, Jianwei Liu, Yang Liu, Xiangyun |
| spellingShingle |
Kou, Jianqiang Zheng, Xiujun Guo, Jianwei Liu, Yang Liu, Xiangyun Journal of Cellular Biochemistry MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells Cell Biology Molecular Biology Biochemistry |
| author_sort |
kou, jianqiang |
| spelling |
Kou, Jianqiang Zheng, Xiujun Guo, Jianwei Liu, Yang Liu, Xiangyun 0730-2312 1097-4644 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1002/jcb.29355 <jats:title>Abstract</jats:title><jats:p>MicroRNAs (miRs) are short noncoding RNAs that play key regulatory roles in osteoblast differentiation. In this study, the specific regulatory roles of miR‐218‐5p on postmenopausal osteoporosis (PMOP) were investigated. The mouse model of PMOP was established by bilateral ovariectomy, and the injection of miR‐218‐5p mimics significantly relieved PMOP degree. Then, bone marrow mesenchymal stem cells (BMMSCs) isolated from PMOP mice were induced into osteoblasts. When compared with normal BMMSCs<jats:bold>,</jats:bold> PMOP BMMSCs exhibited significantly lower alkaline phosphatase (ALP) activity and less mineralized nodules, as well as downregulated miR‐218‐5p, Runx2, Osterix, COL1A1, and OCN after induction (<jats:italic>P</jats:italic> < .05). The transfection of miR‐218‐5p mimics, and inhibitor significantly promoted, inhibited the osteoblast differentiation of PMOP BMMSCs, respectively. In addition, COL1A1 was a target of miR‐218‐5p. The transfection of miR‐218‐5p mimics into PMOP BMMSCs significantly upregulated COL1A1 at 14th and 21st day post‐induction, but not at 7th day. Our findings suggest miR‐218‐5p may relieve PMOP through promoting the osteoblast differentiation of BMMSCs.</jats:p> MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells Journal of Cellular Biochemistry |
| doi_str_mv |
10.1002/jcb.29355 |
| facet_avail |
Online |
| finc_class_facet |
Biologie Chemie und Pharmazie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9qY2IuMjkzNTU |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9qY2IuMjkzNTU |
| institution |
DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Zi4 DE-Gla1 |
| imprint |
Wiley, 2020 |
| imprint_str_mv |
Wiley, 2020 |
| issn |
0730-2312 1097-4644 |
| issn_str_mv |
0730-2312 1097-4644 |
| language |
English |
| mega_collection |
Wiley (CrossRef) |
| match_str |
kou2020microrna2185prelievespostmenopausalosteoporosisthroughpromotingtheosteoblastdifferentiationofbonemarrowmesenchymalstemcells |
| publishDateSort |
2020 |
| publisher |
Wiley |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Cellular Biochemistry |
| source_id |
49 |
| title |
MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| title_unstemmed |
MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| title_full |
MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| title_fullStr |
MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| title_full_unstemmed |
MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| title_short |
MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| title_sort |
microrna‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| topic |
Cell Biology Molecular Biology Biochemistry |
| url |
http://dx.doi.org/10.1002/jcb.29355 |
| publishDate |
2020 |
| physical |
1216-1226 |
| description |
<jats:title>Abstract</jats:title><jats:p>MicroRNAs (miRs) are short noncoding RNAs that play key regulatory roles in osteoblast differentiation. In this study, the specific regulatory roles of miR‐218‐5p on postmenopausal osteoporosis (PMOP) were investigated. The mouse model of PMOP was established by bilateral ovariectomy, and the injection of miR‐218‐5p mimics significantly relieved PMOP degree. Then, bone marrow mesenchymal stem cells (BMMSCs) isolated from PMOP mice were induced into osteoblasts. When compared with normal BMMSCs<jats:bold>,</jats:bold> PMOP BMMSCs exhibited significantly lower alkaline phosphatase (ALP) activity and less mineralized nodules, as well as downregulated miR‐218‐5p, Runx2, Osterix, COL1A1, and OCN after induction (<jats:italic>P</jats:italic> < .05). The transfection of miR‐218‐5p mimics, and inhibitor significantly promoted, inhibited the osteoblast differentiation of PMOP BMMSCs, respectively. In addition, COL1A1 was a target of miR‐218‐5p. The transfection of miR‐218‐5p mimics into PMOP BMMSCs significantly upregulated COL1A1 at 14th and 21st day post‐induction, but not at 7th day. Our findings suggest miR‐218‐5p may relieve PMOP through promoting the osteoblast differentiation of BMMSCs.</jats:p> |
| container_issue |
2 |
| container_start_page |
1216 |
| container_title |
Journal of Cellular Biochemistry |
| container_volume |
121 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792346205169123328 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:35:41.796Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=MicroRNA%E2%80%90218%E2%80%905p+relieves+postmenopausal+osteoporosis+through+promoting+the+osteoblast+differentiation+of+bone+marrow+mesenchymal+stem+cells&rft.date=2020-02-01&genre=article&issn=1097-4644&volume=121&issue=2&spage=1216&epage=1226&pages=1216-1226&jtitle=Journal+of+Cellular+Biochemistry&atitle=MicroRNA%E2%80%90218%E2%80%905p+relieves+postmenopausal+osteoporosis+through+promoting+the+osteoblast+differentiation+of+bone+marrow+mesenchymal+stem+cells&aulast=Liu&aufirst=Xiangyun&rft_id=info%3Adoi%2F10.1002%2Fjcb.29355&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792346205169123328 |
| author | Kou, Jianqiang, Zheng, Xiujun, Guo, Jianwei, Liu, Yang, Liu, Xiangyun |
| author_facet | Kou, Jianqiang, Zheng, Xiujun, Guo, Jianwei, Liu, Yang, Liu, Xiangyun, Kou, Jianqiang, Zheng, Xiujun, Guo, Jianwei, Liu, Yang, Liu, Xiangyun |
| author_sort | kou, jianqiang |
| container_issue | 2 |
| container_start_page | 1216 |
| container_title | Journal of Cellular Biochemistry |
| container_volume | 121 |
| description | <jats:title>Abstract</jats:title><jats:p>MicroRNAs (miRs) are short noncoding RNAs that play key regulatory roles in osteoblast differentiation. In this study, the specific regulatory roles of miR‐218‐5p on postmenopausal osteoporosis (PMOP) were investigated. The mouse model of PMOP was established by bilateral ovariectomy, and the injection of miR‐218‐5p mimics significantly relieved PMOP degree. Then, bone marrow mesenchymal stem cells (BMMSCs) isolated from PMOP mice were induced into osteoblasts. When compared with normal BMMSCs<jats:bold>,</jats:bold> PMOP BMMSCs exhibited significantly lower alkaline phosphatase (ALP) activity and less mineralized nodules, as well as downregulated miR‐218‐5p, Runx2, Osterix, COL1A1, and OCN after induction (<jats:italic>P</jats:italic> < .05). The transfection of miR‐218‐5p mimics, and inhibitor significantly promoted, inhibited the osteoblast differentiation of PMOP BMMSCs, respectively. In addition, COL1A1 was a target of miR‐218‐5p. The transfection of miR‐218‐5p mimics into PMOP BMMSCs significantly upregulated COL1A1 at 14th and 21st day post‐induction, but not at 7th day. Our findings suggest miR‐218‐5p may relieve PMOP through promoting the osteoblast differentiation of BMMSCs.</jats:p> |
| doi_str_mv | 10.1002/jcb.29355 |
| facet_avail | Online |
| finc_class_facet | Biologie, Chemie und Pharmazie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9qY2IuMjkzNTU |
| imprint | Wiley, 2020 |
| imprint_str_mv | Wiley, 2020 |
| institution | DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zi4, DE-Gla1 |
| issn | 0730-2312, 1097-4644 |
| issn_str_mv | 0730-2312, 1097-4644 |
| language | English |
| last_indexed | 2024-03-01T17:35:41.796Z |
| match_str | kou2020microrna2185prelievespostmenopausalosteoporosisthroughpromotingtheosteoblastdifferentiationofbonemarrowmesenchymalstemcells |
| mega_collection | Wiley (CrossRef) |
| physical | 1216-1226 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | Journal of Cellular Biochemistry |
| source_id | 49 |
| spelling | Kou, Jianqiang Zheng, Xiujun Guo, Jianwei Liu, Yang Liu, Xiangyun 0730-2312 1097-4644 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1002/jcb.29355 <jats:title>Abstract</jats:title><jats:p>MicroRNAs (miRs) are short noncoding RNAs that play key regulatory roles in osteoblast differentiation. In this study, the specific regulatory roles of miR‐218‐5p on postmenopausal osteoporosis (PMOP) were investigated. The mouse model of PMOP was established by bilateral ovariectomy, and the injection of miR‐218‐5p mimics significantly relieved PMOP degree. Then, bone marrow mesenchymal stem cells (BMMSCs) isolated from PMOP mice were induced into osteoblasts. When compared with normal BMMSCs<jats:bold>,</jats:bold> PMOP BMMSCs exhibited significantly lower alkaline phosphatase (ALP) activity and less mineralized nodules, as well as downregulated miR‐218‐5p, Runx2, Osterix, COL1A1, and OCN after induction (<jats:italic>P</jats:italic> < .05). The transfection of miR‐218‐5p mimics, and inhibitor significantly promoted, inhibited the osteoblast differentiation of PMOP BMMSCs, respectively. In addition, COL1A1 was a target of miR‐218‐5p. The transfection of miR‐218‐5p mimics into PMOP BMMSCs significantly upregulated COL1A1 at 14th and 21st day post‐induction, but not at 7th day. Our findings suggest miR‐218‐5p may relieve PMOP through promoting the osteoblast differentiation of BMMSCs.</jats:p> MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells Journal of Cellular Biochemistry |
| spellingShingle | Kou, Jianqiang, Zheng, Xiujun, Guo, Jianwei, Liu, Yang, Liu, Xiangyun, Journal of Cellular Biochemistry, MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells, Cell Biology, Molecular Biology, Biochemistry |
| title | MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| title_full | MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| title_fullStr | MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| title_full_unstemmed | MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| title_short | MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| title_sort | microrna‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| title_unstemmed | MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells |
| topic | Cell Biology, Molecular Biology, Biochemistry |
| url | http://dx.doi.org/10.1002/jcb.29355 |