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NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway
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| Zeitschriftentitel: | Journal of Cellular Biochemistry |
|---|---|
| Personen und Körperschaften: | , , , , , , , , |
| In: | Journal of Cellular Biochemistry, 121, 2020, 2, S. 1192-1204 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Li, Jing Yang, Rui Yang, Haijie Chen, Sujuan Wang, Lei Li, Man Yang, Shaokui Feng, Zhiwei Bi, Jiajia Li, Jing Yang, Rui Yang, Haijie Chen, Sujuan Wang, Lei Li, Man Yang, Shaokui Feng, Zhiwei Bi, Jiajia |
|---|---|
| author |
Li, Jing Yang, Rui Yang, Haijie Chen, Sujuan Wang, Lei Li, Man Yang, Shaokui Feng, Zhiwei Bi, Jiajia |
| spellingShingle |
Li, Jing Yang, Rui Yang, Haijie Chen, Sujuan Wang, Lei Li, Man Yang, Shaokui Feng, Zhiwei Bi, Jiajia Journal of Cellular Biochemistry NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway Cell Biology Molecular Biology Biochemistry |
| author_sort |
li, jing |
| spelling |
Li, Jing Yang, Rui Yang, Haijie Chen, Sujuan Wang, Lei Li, Man Yang, Shaokui Feng, Zhiwei Bi, Jiajia 0730-2312 1097-4644 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1002/jcb.29353 <jats:title>Abstract</jats:title><jats:p>The neural cell adhesion molecule (NCAM) plays critical roles in multiple cellular processes in neural cells, mesenchymal stem cells, and various cancer cells. However, the effect and mechanism of NCAM in human melanoma cells are still unclear. In this study, we found that NCAM regulated the proliferation, apoptosis, autophagy, migration, and epithelial‐to‐mesenchymal transition of human melanoma cells by determining the biological behavior of NCAM knockdown A375 and M102 human melanoma cells. Further studies revealed that NCAM knockdown impaired the organization of actin cytoskeleton and reduced the phosphorylation of cofilin, an actin‐cleaving protein. When cells were transfected with cofilin S3A (dephosphorylated cofilin), biological behavior similar to that of NCAM knockdown cells was observed. Research on the underlying molecular mechanism showed that NCAM knockdown suppressed activation of the Src/Akt/mTOR pathway. Specific inhibitors of Src and PI3K/Akt were employed to further verify the relationship between Src/Akt/mTOR signaling and cofilin, and the results showed that the phosphorylation level of cofilin decreased following inhibition of the Src/Akt/mTOR pathway. These results indicated that NCAM may regulate the proliferation, apoptosis, autophagy, migration, and epithelial‐to‐mesenchymal transition of human melanoma cells via the Src/Akt/mTOR/cofilin pathway‐mediated dynamics of actin cytoskeleton.</jats:p> NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway Journal of Cellular Biochemistry |
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Wiley, 2020 |
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| title |
NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway |
| title_unstemmed |
NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway |
| title_full |
NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway |
| title_fullStr |
NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway |
| title_full_unstemmed |
NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway |
| title_short |
NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway |
| title_sort |
ncam regulates the proliferation, apoptosis, autophagy, emt, and migration of human melanoma cells via the src/akt/mtor/cofilin signaling pathway |
| topic |
Cell Biology Molecular Biology Biochemistry |
| url |
http://dx.doi.org/10.1002/jcb.29353 |
| publishDate |
2020 |
| physical |
1192-1204 |
| description |
<jats:title>Abstract</jats:title><jats:p>The neural cell adhesion molecule (NCAM) plays critical roles in multiple cellular processes in neural cells, mesenchymal stem cells, and various cancer cells. However, the effect and mechanism of NCAM in human melanoma cells are still unclear. In this study, we found that NCAM regulated the proliferation, apoptosis, autophagy, migration, and epithelial‐to‐mesenchymal transition of human melanoma cells by determining the biological behavior of NCAM knockdown A375 and M102 human melanoma cells. Further studies revealed that NCAM knockdown impaired the organization of actin cytoskeleton and reduced the phosphorylation of cofilin, an actin‐cleaving protein. When cells were transfected with cofilin S3A (dephosphorylated cofilin), biological behavior similar to that of NCAM knockdown cells was observed. Research on the underlying molecular mechanism showed that NCAM knockdown suppressed activation of the Src/Akt/mTOR pathway. Specific inhibitors of Src and PI3K/Akt were employed to further verify the relationship between Src/Akt/mTOR signaling and cofilin, and the results showed that the phosphorylation level of cofilin decreased following inhibition of the Src/Akt/mTOR pathway. These results indicated that NCAM may regulate the proliferation, apoptosis, autophagy, migration, and epithelial‐to‐mesenchymal transition of human melanoma cells via the Src/Akt/mTOR/cofilin pathway‐mediated dynamics of actin cytoskeleton.</jats:p> |
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| author | Li, Jing, Yang, Rui, Yang, Haijie, Chen, Sujuan, Wang, Lei, Li, Man, Yang, Shaokui, Feng, Zhiwei, Bi, Jiajia |
| author_facet | Li, Jing, Yang, Rui, Yang, Haijie, Chen, Sujuan, Wang, Lei, Li, Man, Yang, Shaokui, Feng, Zhiwei, Bi, Jiajia, Li, Jing, Yang, Rui, Yang, Haijie, Chen, Sujuan, Wang, Lei, Li, Man, Yang, Shaokui, Feng, Zhiwei, Bi, Jiajia |
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| description | <jats:title>Abstract</jats:title><jats:p>The neural cell adhesion molecule (NCAM) plays critical roles in multiple cellular processes in neural cells, mesenchymal stem cells, and various cancer cells. However, the effect and mechanism of NCAM in human melanoma cells are still unclear. In this study, we found that NCAM regulated the proliferation, apoptosis, autophagy, migration, and epithelial‐to‐mesenchymal transition of human melanoma cells by determining the biological behavior of NCAM knockdown A375 and M102 human melanoma cells. Further studies revealed that NCAM knockdown impaired the organization of actin cytoskeleton and reduced the phosphorylation of cofilin, an actin‐cleaving protein. When cells were transfected with cofilin S3A (dephosphorylated cofilin), biological behavior similar to that of NCAM knockdown cells was observed. Research on the underlying molecular mechanism showed that NCAM knockdown suppressed activation of the Src/Akt/mTOR pathway. Specific inhibitors of Src and PI3K/Akt were employed to further verify the relationship between Src/Akt/mTOR signaling and cofilin, and the results showed that the phosphorylation level of cofilin decreased following inhibition of the Src/Akt/mTOR pathway. These results indicated that NCAM may regulate the proliferation, apoptosis, autophagy, migration, and epithelial‐to‐mesenchymal transition of human melanoma cells via the Src/Akt/mTOR/cofilin pathway‐mediated dynamics of actin cytoskeleton.</jats:p> |
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| spelling | Li, Jing Yang, Rui Yang, Haijie Chen, Sujuan Wang, Lei Li, Man Yang, Shaokui Feng, Zhiwei Bi, Jiajia 0730-2312 1097-4644 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1002/jcb.29353 <jats:title>Abstract</jats:title><jats:p>The neural cell adhesion molecule (NCAM) plays critical roles in multiple cellular processes in neural cells, mesenchymal stem cells, and various cancer cells. However, the effect and mechanism of NCAM in human melanoma cells are still unclear. In this study, we found that NCAM regulated the proliferation, apoptosis, autophagy, migration, and epithelial‐to‐mesenchymal transition of human melanoma cells by determining the biological behavior of NCAM knockdown A375 and M102 human melanoma cells. Further studies revealed that NCAM knockdown impaired the organization of actin cytoskeleton and reduced the phosphorylation of cofilin, an actin‐cleaving protein. When cells were transfected with cofilin S3A (dephosphorylated cofilin), biological behavior similar to that of NCAM knockdown cells was observed. Research on the underlying molecular mechanism showed that NCAM knockdown suppressed activation of the Src/Akt/mTOR pathway. Specific inhibitors of Src and PI3K/Akt were employed to further verify the relationship between Src/Akt/mTOR signaling and cofilin, and the results showed that the phosphorylation level of cofilin decreased following inhibition of the Src/Akt/mTOR pathway. These results indicated that NCAM may regulate the proliferation, apoptosis, autophagy, migration, and epithelial‐to‐mesenchymal transition of human melanoma cells via the Src/Akt/mTOR/cofilin pathway‐mediated dynamics of actin cytoskeleton.</jats:p> NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway Journal of Cellular Biochemistry |
| spellingShingle | Li, Jing, Yang, Rui, Yang, Haijie, Chen, Sujuan, Wang, Lei, Li, Man, Yang, Shaokui, Feng, Zhiwei, Bi, Jiajia, Journal of Cellular Biochemistry, NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway, Cell Biology, Molecular Biology, Biochemistry |
| title | NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway |
| title_full | NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway |
| title_fullStr | NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway |
| title_full_unstemmed | NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway |
| title_short | NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway |
| title_sort | ncam regulates the proliferation, apoptosis, autophagy, emt, and migration of human melanoma cells via the src/akt/mtor/cofilin signaling pathway |
| title_unstemmed | NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway |
| topic | Cell Biology, Molecular Biology, Biochemistry |
| url | http://dx.doi.org/10.1002/jcb.29353 |