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TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3
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| Zeitschriftentitel: | Journal of Cellular Biochemistry |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Journal of Cellular Biochemistry, 121, 2020, 2, S. 1101-1113 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Gu, Ke Fu, Xucheng Tian, Hui Zhang, Yafei Li, Aonan Wang, Ying Wen, Yong Gu, Weiting Gu, Ke Fu, Xucheng Tian, Hui Zhang, Yafei Li, Aonan Wang, Ying Wen, Yong Gu, Weiting |
|---|---|
| author |
Gu, Ke Fu, Xucheng Tian, Hui Zhang, Yafei Li, Aonan Wang, Ying Wen, Yong Gu, Weiting |
| spellingShingle |
Gu, Ke Fu, Xucheng Tian, Hui Zhang, Yafei Li, Aonan Wang, Ying Wen, Yong Gu, Weiting Journal of Cellular Biochemistry TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 Cell Biology Molecular Biology Biochemistry |
| author_sort |
gu, ke |
| spelling |
Gu, Ke Fu, Xucheng Tian, Hui Zhang, Yafei Li, Aonan Wang, Ying Wen, Yong Gu, Weiting 0730-2312 1097-4644 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1002/jcb.29346 <jats:title>Abstract</jats:title><jats:sec><jats:title>Objective</jats:title><jats:p>This study aims to offer insights about the biological influence of TAZ, which is a transcriptional coactivator containing a PDZ‐binding motif, upon the apoptosis, proliferation, and osteogenic differentiation of human periodontal ligament stem cells (h‐PDLSCs).</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>We used the green fluorescence protein lentivirus infection system to knockdown or overexpress TAZ in h‐PDLSCs. 5‐ethynyl‐2’‐deoxyuridine (EdU) staining detected the proliferative activity, and h‐PDLSC apoptosis was analyzed by Annexin V‐APC staining. TAZ knockdown or overexpression was performed to determine the osteogenic differentiation function of TAZ during the osteogenic induction of h‐PDLSCs. The molecular mechanism of TAZ in the promotion of h‐PDLSC osteogenesis was also explored. The chemical inhibitor of SMAD2/3 SIS3 HCL was used to identify the effects in vitro osteogenic differentiation and bone formation in h‐PDLSCs overexpressing TAZ.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>TAZ overexpression resulted in enhanced cell rapid multiplication, which increased the expression of messenger RNA in stemness‐related genes. By comparison, TAZ knockdown reduced proliferative activity and increased the apoptosis of h‐PDLSCs. After the 7‐day osteogenic induction period, alkaline phosphatase activity in the TAZ‐overexpression group was significantly increased, and mineralized nodules increased significantly after osteogenic induction for 21 days. Similarly, osteoblast differentiation of h‐PDLSCs was impaired after TAZ knockdown. However, the osteogenic potential of the group exposed to the p‐SMAD3 inhibitor was restored to its original level.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>Hippo/TAZ plays a positive role inside the proliferation, stemness maintenance, and osteogenic specialization of h‐PDLSCs, and the specific downstream factor of osteogenic differentiation is SMAD3.</jats:p></jats:sec> TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 Journal of Cellular Biochemistry |
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10.1002/jcb.29346 |
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Online |
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Biologie Chemie und Pharmazie |
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Wiley, 2020 |
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Wiley, 2020 |
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2020 |
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Journal of Cellular Biochemistry |
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| title |
TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 |
| title_unstemmed |
TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 |
| title_full |
TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 |
| title_fullStr |
TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 |
| title_full_unstemmed |
TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 |
| title_short |
TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 |
| title_sort |
taz promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐smad3 |
| topic |
Cell Biology Molecular Biology Biochemistry |
| url |
http://dx.doi.org/10.1002/jcb.29346 |
| publishDate |
2020 |
| physical |
1101-1113 |
| description |
<jats:title>Abstract</jats:title><jats:sec><jats:title>Objective</jats:title><jats:p>This study aims to offer insights about the biological influence of TAZ, which is a transcriptional coactivator containing a PDZ‐binding motif, upon the apoptosis, proliferation, and osteogenic differentiation of human periodontal ligament stem cells (h‐PDLSCs).</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>We used the green fluorescence protein lentivirus infection system to knockdown or overexpress TAZ in h‐PDLSCs. 5‐ethynyl‐2’‐deoxyuridine (EdU) staining detected the proliferative activity, and h‐PDLSC apoptosis was analyzed by Annexin V‐APC staining. TAZ knockdown or overexpression was performed to determine the osteogenic differentiation function of TAZ during the osteogenic induction of h‐PDLSCs. The molecular mechanism of TAZ in the promotion of h‐PDLSC osteogenesis was also explored. The chemical inhibitor of SMAD2/3 SIS3 HCL was used to identify the effects in vitro osteogenic differentiation and bone formation in h‐PDLSCs overexpressing TAZ.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>TAZ overexpression resulted in enhanced cell rapid multiplication, which increased the expression of messenger RNA in stemness‐related genes. By comparison, TAZ knockdown reduced proliferative activity and increased the apoptosis of h‐PDLSCs. After the 7‐day osteogenic induction period, alkaline phosphatase activity in the TAZ‐overexpression group was significantly increased, and mineralized nodules increased significantly after osteogenic induction for 21 days. Similarly, osteoblast differentiation of h‐PDLSCs was impaired after TAZ knockdown. However, the osteogenic potential of the group exposed to the p‐SMAD3 inhibitor was restored to its original level.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>Hippo/TAZ plays a positive role inside the proliferation, stemness maintenance, and osteogenic specialization of h‐PDLSCs, and the specific downstream factor of osteogenic differentiation is SMAD3.</jats:p></jats:sec> |
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| _version_ | 1792345448120320012 |
| author | Gu, Ke, Fu, Xucheng, Tian, Hui, Zhang, Yafei, Li, Aonan, Wang, Ying, Wen, Yong, Gu, Weiting |
| author_facet | Gu, Ke, Fu, Xucheng, Tian, Hui, Zhang, Yafei, Li, Aonan, Wang, Ying, Wen, Yong, Gu, Weiting, Gu, Ke, Fu, Xucheng, Tian, Hui, Zhang, Yafei, Li, Aonan, Wang, Ying, Wen, Yong, Gu, Weiting |
| author_sort | gu, ke |
| container_issue | 2 |
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| description | <jats:title>Abstract</jats:title><jats:sec><jats:title>Objective</jats:title><jats:p>This study aims to offer insights about the biological influence of TAZ, which is a transcriptional coactivator containing a PDZ‐binding motif, upon the apoptosis, proliferation, and osteogenic differentiation of human periodontal ligament stem cells (h‐PDLSCs).</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>We used the green fluorescence protein lentivirus infection system to knockdown or overexpress TAZ in h‐PDLSCs. 5‐ethynyl‐2’‐deoxyuridine (EdU) staining detected the proliferative activity, and h‐PDLSC apoptosis was analyzed by Annexin V‐APC staining. TAZ knockdown or overexpression was performed to determine the osteogenic differentiation function of TAZ during the osteogenic induction of h‐PDLSCs. The molecular mechanism of TAZ in the promotion of h‐PDLSC osteogenesis was also explored. The chemical inhibitor of SMAD2/3 SIS3 HCL was used to identify the effects in vitro osteogenic differentiation and bone formation in h‐PDLSCs overexpressing TAZ.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>TAZ overexpression resulted in enhanced cell rapid multiplication, which increased the expression of messenger RNA in stemness‐related genes. By comparison, TAZ knockdown reduced proliferative activity and increased the apoptosis of h‐PDLSCs. After the 7‐day osteogenic induction period, alkaline phosphatase activity in the TAZ‐overexpression group was significantly increased, and mineralized nodules increased significantly after osteogenic induction for 21 days. Similarly, osteoblast differentiation of h‐PDLSCs was impaired after TAZ knockdown. However, the osteogenic potential of the group exposed to the p‐SMAD3 inhibitor was restored to its original level.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>Hippo/TAZ plays a positive role inside the proliferation, stemness maintenance, and osteogenic specialization of h‐PDLSCs, and the specific downstream factor of osteogenic differentiation is SMAD3.</jats:p></jats:sec> |
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| imprint | Wiley, 2020 |
| imprint_str_mv | Wiley, 2020 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| series | Journal of Cellular Biochemistry |
| source_id | 49 |
| spelling | Gu, Ke Fu, Xucheng Tian, Hui Zhang, Yafei Li, Aonan Wang, Ying Wen, Yong Gu, Weiting 0730-2312 1097-4644 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1002/jcb.29346 <jats:title>Abstract</jats:title><jats:sec><jats:title>Objective</jats:title><jats:p>This study aims to offer insights about the biological influence of TAZ, which is a transcriptional coactivator containing a PDZ‐binding motif, upon the apoptosis, proliferation, and osteogenic differentiation of human periodontal ligament stem cells (h‐PDLSCs).</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>We used the green fluorescence protein lentivirus infection system to knockdown or overexpress TAZ in h‐PDLSCs. 5‐ethynyl‐2’‐deoxyuridine (EdU) staining detected the proliferative activity, and h‐PDLSC apoptosis was analyzed by Annexin V‐APC staining. TAZ knockdown or overexpression was performed to determine the osteogenic differentiation function of TAZ during the osteogenic induction of h‐PDLSCs. The molecular mechanism of TAZ in the promotion of h‐PDLSC osteogenesis was also explored. The chemical inhibitor of SMAD2/3 SIS3 HCL was used to identify the effects in vitro osteogenic differentiation and bone formation in h‐PDLSCs overexpressing TAZ.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>TAZ overexpression resulted in enhanced cell rapid multiplication, which increased the expression of messenger RNA in stemness‐related genes. By comparison, TAZ knockdown reduced proliferative activity and increased the apoptosis of h‐PDLSCs. After the 7‐day osteogenic induction period, alkaline phosphatase activity in the TAZ‐overexpression group was significantly increased, and mineralized nodules increased significantly after osteogenic induction for 21 days. Similarly, osteoblast differentiation of h‐PDLSCs was impaired after TAZ knockdown. However, the osteogenic potential of the group exposed to the p‐SMAD3 inhibitor was restored to its original level.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>Hippo/TAZ plays a positive role inside the proliferation, stemness maintenance, and osteogenic specialization of h‐PDLSCs, and the specific downstream factor of osteogenic differentiation is SMAD3.</jats:p></jats:sec> TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 Journal of Cellular Biochemistry |
| spellingShingle | Gu, Ke, Fu, Xucheng, Tian, Hui, Zhang, Yafei, Li, Aonan, Wang, Ying, Wen, Yong, Gu, Weiting, Journal of Cellular Biochemistry, TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3, Cell Biology, Molecular Biology, Biochemistry |
| title | TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 |
| title_full | TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 |
| title_fullStr | TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 |
| title_full_unstemmed | TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 |
| title_short | TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 |
| title_sort | taz promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐smad3 |
| title_unstemmed | TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3 |
| topic | Cell Biology, Molecular Biology, Biochemistry |
| url | http://dx.doi.org/10.1002/jcb.29346 |