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ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2
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| Zeitschriftentitel: | Journal of Cellular Biochemistry |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , |
| In: | Journal of Cellular Biochemistry, 121, 2020, 1, S. 609-620 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Zhang, Zhe Wang, Wei Liu, Jian‐Bo Wang, Ying Hao, Jin‐Dong Huang, Yi‐Jie Gao, Yan Jiang, Hao Yuan, Bao Zhang, Jia‐Bao Zhang, Zhe Wang, Wei Liu, Jian‐Bo Wang, Ying Hao, Jin‐Dong Huang, Yi‐Jie Gao, Yan Jiang, Hao Yuan, Bao Zhang, Jia‐Bao |
|---|---|
| author |
Zhang, Zhe Wang, Wei Liu, Jian‐Bo Wang, Ying Hao, Jin‐Dong Huang, Yi‐Jie Gao, Yan Jiang, Hao Yuan, Bao Zhang, Jia‐Bao |
| spellingShingle |
Zhang, Zhe Wang, Wei Liu, Jian‐Bo Wang, Ying Hao, Jin‐Dong Huang, Yi‐Jie Gao, Yan Jiang, Hao Yuan, Bao Zhang, Jia‐Bao Journal of Cellular Biochemistry ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 Cell Biology Molecular Biology Biochemistry |
| author_sort |
zhang, zhe |
| spelling |
Zhang, Zhe Wang, Wei Liu, Jian‐Bo Wang, Ying Hao, Jin‐Dong Huang, Yi‐Jie Gao, Yan Jiang, Hao Yuan, Bao Zhang, Jia‐Bao 0730-2312 1097-4644 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1002/jcb.29306 <jats:title>Abstract</jats:title><jats:p>MicroRNAs (miRNAs) take part in a variety of biological processes by regulating target genes. Transforming growth factor β receptor 1 (TGFBR1) and TGFBR2 are crucial members of the TGF‐β family and are serine/threonine kinase receptors. The aim of this study was to explore the functions of ssc‐miR‐204 in porcine preadipocyte differentiation and apoptosis with regard to the TGFβ/Smad pathway. We identified miRNAs predicted to target TGFBR1 and TGFBR2 using a database and selected ssc‐miR‐204 as a candidate miRNA. ssc‐miR‐204 overexpression dramatically reduced the levels of TGFBR1 and TGFBR2. However, after transfection with ssc‐miR‐204 inhibitor, TGFBR1 and TGFBR2 levels were dramatically increased. ssc‐miR‐204 overexpression dramatically promoted porcine preadipocyte differentiation and apoptosis. After transfection with ssc‐miR‐204 inhibitor, porcine preadipocyte differentiation and apoptosis were dramatically inhibited. After transfection with ssc‐miR‐204 mimics, Smad2, Smad3, Smad4, p‐Smad2, and p‐Smad3 protein levels significantly decreased, and adipogenesis was regulated by inhibiting the TGF‐β/Smad3 signaling pathway. Taken together, these results verified that ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2.</jats:p> ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 Journal of Cellular Biochemistry |
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Wiley, 2020 |
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Wiley, 2020 |
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0730-2312 1097-4644 |
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Journal of Cellular Biochemistry |
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| title |
ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 |
| title_unstemmed |
ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 |
| title_full |
ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 |
| title_fullStr |
ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 |
| title_full_unstemmed |
ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 |
| title_short |
ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 |
| title_sort |
ssc‐mir‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting tgfbr1 and tgfbr2 |
| topic |
Cell Biology Molecular Biology Biochemistry |
| url |
http://dx.doi.org/10.1002/jcb.29306 |
| publishDate |
2020 |
| physical |
609-620 |
| description |
<jats:title>Abstract</jats:title><jats:p>MicroRNAs (miRNAs) take part in a variety of biological processes by regulating target genes. Transforming growth factor β receptor 1 (TGFBR1) and TGFBR2 are crucial members of the TGF‐β family and are serine/threonine kinase receptors. The aim of this study was to explore the functions of ssc‐miR‐204 in porcine preadipocyte differentiation and apoptosis with regard to the TGFβ/Smad pathway. We identified miRNAs predicted to target TGFBR1 and TGFBR2 using a database and selected ssc‐miR‐204 as a candidate miRNA. ssc‐miR‐204 overexpression dramatically reduced the levels of TGFBR1 and TGFBR2. However, after transfection with ssc‐miR‐204 inhibitor, TGFBR1 and TGFBR2 levels were dramatically increased. ssc‐miR‐204 overexpression dramatically promoted porcine preadipocyte differentiation and apoptosis. After transfection with ssc‐miR‐204 inhibitor, porcine preadipocyte differentiation and apoptosis were dramatically inhibited. After transfection with ssc‐miR‐204 mimics, Smad2, Smad3, Smad4, p‐Smad2, and p‐Smad3 protein levels significantly decreased, and adipogenesis was regulated by inhibiting the TGF‐β/Smad3 signaling pathway. Taken together, these results verified that ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2.</jats:p> |
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| author | Zhang, Zhe, Wang, Wei, Liu, Jian‐Bo, Wang, Ying, Hao, Jin‐Dong, Huang, Yi‐Jie, Gao, Yan, Jiang, Hao, Yuan, Bao, Zhang, Jia‐Bao |
| author_facet | Zhang, Zhe, Wang, Wei, Liu, Jian‐Bo, Wang, Ying, Hao, Jin‐Dong, Huang, Yi‐Jie, Gao, Yan, Jiang, Hao, Yuan, Bao, Zhang, Jia‐Bao, Zhang, Zhe, Wang, Wei, Liu, Jian‐Bo, Wang, Ying, Hao, Jin‐Dong, Huang, Yi‐Jie, Gao, Yan, Jiang, Hao, Yuan, Bao, Zhang, Jia‐Bao |
| author_sort | zhang, zhe |
| container_issue | 1 |
| container_start_page | 609 |
| container_title | Journal of Cellular Biochemistry |
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| description | <jats:title>Abstract</jats:title><jats:p>MicroRNAs (miRNAs) take part in a variety of biological processes by regulating target genes. Transforming growth factor β receptor 1 (TGFBR1) and TGFBR2 are crucial members of the TGF‐β family and are serine/threonine kinase receptors. The aim of this study was to explore the functions of ssc‐miR‐204 in porcine preadipocyte differentiation and apoptosis with regard to the TGFβ/Smad pathway. We identified miRNAs predicted to target TGFBR1 and TGFBR2 using a database and selected ssc‐miR‐204 as a candidate miRNA. ssc‐miR‐204 overexpression dramatically reduced the levels of TGFBR1 and TGFBR2. However, after transfection with ssc‐miR‐204 inhibitor, TGFBR1 and TGFBR2 levels were dramatically increased. ssc‐miR‐204 overexpression dramatically promoted porcine preadipocyte differentiation and apoptosis. After transfection with ssc‐miR‐204 inhibitor, porcine preadipocyte differentiation and apoptosis were dramatically inhibited. After transfection with ssc‐miR‐204 mimics, Smad2, Smad3, Smad4, p‐Smad2, and p‐Smad3 protein levels significantly decreased, and adipogenesis was regulated by inhibiting the TGF‐β/Smad3 signaling pathway. Taken together, these results verified that ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2.</jats:p> |
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| institution | DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
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| spelling | Zhang, Zhe Wang, Wei Liu, Jian‐Bo Wang, Ying Hao, Jin‐Dong Huang, Yi‐Jie Gao, Yan Jiang, Hao Yuan, Bao Zhang, Jia‐Bao 0730-2312 1097-4644 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1002/jcb.29306 <jats:title>Abstract</jats:title><jats:p>MicroRNAs (miRNAs) take part in a variety of biological processes by regulating target genes. Transforming growth factor β receptor 1 (TGFBR1) and TGFBR2 are crucial members of the TGF‐β family and are serine/threonine kinase receptors. The aim of this study was to explore the functions of ssc‐miR‐204 in porcine preadipocyte differentiation and apoptosis with regard to the TGFβ/Smad pathway. We identified miRNAs predicted to target TGFBR1 and TGFBR2 using a database and selected ssc‐miR‐204 as a candidate miRNA. ssc‐miR‐204 overexpression dramatically reduced the levels of TGFBR1 and TGFBR2. However, after transfection with ssc‐miR‐204 inhibitor, TGFBR1 and TGFBR2 levels were dramatically increased. ssc‐miR‐204 overexpression dramatically promoted porcine preadipocyte differentiation and apoptosis. After transfection with ssc‐miR‐204 inhibitor, porcine preadipocyte differentiation and apoptosis were dramatically inhibited. After transfection with ssc‐miR‐204 mimics, Smad2, Smad3, Smad4, p‐Smad2, and p‐Smad3 protein levels significantly decreased, and adipogenesis was regulated by inhibiting the TGF‐β/Smad3 signaling pathway. Taken together, these results verified that ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2.</jats:p> ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 Journal of Cellular Biochemistry |
| spellingShingle | Zhang, Zhe, Wang, Wei, Liu, Jian‐Bo, Wang, Ying, Hao, Jin‐Dong, Huang, Yi‐Jie, Gao, Yan, Jiang, Hao, Yuan, Bao, Zhang, Jia‐Bao, Journal of Cellular Biochemistry, ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2, Cell Biology, Molecular Biology, Biochemistry |
| title | ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 |
| title_full | ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 |
| title_fullStr | ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 |
| title_full_unstemmed | ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 |
| title_short | ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 |
| title_sort | ssc‐mir‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting tgfbr1 and tgfbr2 |
| title_unstemmed | ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2 |
| topic | Cell Biology, Molecular Biology, Biochemistry |
| url | http://dx.doi.org/10.1002/jcb.29306 |