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MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2
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| Zeitschriftentitel: | Journal of Cellular Biochemistry |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Journal of Cellular Biochemistry, 121, 2020, 2, S. 2038-2046 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Wang, Lei Hu, Kejun Chao, Yu Wang, Xueli Wang, Lei Hu, Kejun Chao, Yu Wang, Xueli |
|---|---|
| author |
Wang, Lei Hu, Kejun Chao, Yu Wang, Xueli |
| spellingShingle |
Wang, Lei Hu, Kejun Chao, Yu Wang, Xueli Journal of Cellular Biochemistry MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 Cell Biology Molecular Biology Biochemistry |
| author_sort |
wang, lei |
| spelling |
Wang, Lei Hu, Kejun Chao, Yu Wang, Xueli 0730-2312 1097-4644 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1002/jcb.29438 <jats:title>Abstract</jats:title><jats:p>Osteosarcoma (OS) is a highly aggressive bone tumor with a poor prognosis. MicroRNAs are revealed to exerts essential roles in the carcinogenesis and tumor invasion of OS. But, the function of miR‐1296‐5p and its related mechanism in OS progression have not yet been studied. This study discovered the levels of miR‐1296‐5p in OS and corresponding noncancerous tissues, and we demonstrated that miR‐1296‐5p level was markedly downregulated in tumor specimens as compared with nontumor tissues. In addition, we discovered that miR‐1296‐5p was also underexpressed in OS cells compared with the hFOB1.19 osteoblast cells. Interestingly, the reduced expression of miR‐1296‐5p was confirmed to associated with large tumor size, advanced tumor stages, and distance metastasis, respectively. Patients with OS low‐expressing miR‐1296‐5p showed a prominent shorter survival. In addition, gain‐of‐function assays verified that miR‐1296‐5p overexpression remarkably repressed OS cell proliferation, migration, and invasion. Conversely, depletion of miR‐1296‐5p facilitated the growth and mobility of OS cells. Notably, miR‐1296‐5p inversely modulated notch receptor 2 (NOTCH2) in OS cells. The level of NOTCH2 messenger RNA was negatively correlated with miR‐1296‐5p level in OS samples. NOTCH2 knockdown markedly suppressed the abilities of MG‐63 cell proliferation and mobility. More importantly, the restoration of NOTCH2 prominently rescued miR‐1296‐5p‐induced tumor‐suppressive effects on MG‐63 cells. In conclusion, our study identified the reduced expression of miR‐1296‐5p, which contributed to OS progression. miR‐1296‐5p might be a promising prognostic marker and therapeutic target in OS.</jats:p> MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 Journal of Cellular Biochemistry |
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Wiley, 2020 |
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Journal of Cellular Biochemistry |
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| title |
MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 |
| title_unstemmed |
MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 |
| title_full |
MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 |
| title_fullStr |
MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 |
| title_full_unstemmed |
MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 |
| title_short |
MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 |
| title_sort |
microrna‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting notch2 |
| topic |
Cell Biology Molecular Biology Biochemistry |
| url |
http://dx.doi.org/10.1002/jcb.29438 |
| publishDate |
2020 |
| physical |
2038-2046 |
| description |
<jats:title>Abstract</jats:title><jats:p>Osteosarcoma (OS) is a highly aggressive bone tumor with a poor prognosis. MicroRNAs are revealed to exerts essential roles in the carcinogenesis and tumor invasion of OS. But, the function of miR‐1296‐5p and its related mechanism in OS progression have not yet been studied. This study discovered the levels of miR‐1296‐5p in OS and corresponding noncancerous tissues, and we demonstrated that miR‐1296‐5p level was markedly downregulated in tumor specimens as compared with nontumor tissues. In addition, we discovered that miR‐1296‐5p was also underexpressed in OS cells compared with the hFOB1.19 osteoblast cells. Interestingly, the reduced expression of miR‐1296‐5p was confirmed to associated with large tumor size, advanced tumor stages, and distance metastasis, respectively. Patients with OS low‐expressing miR‐1296‐5p showed a prominent shorter survival. In addition, gain‐of‐function assays verified that miR‐1296‐5p overexpression remarkably repressed OS cell proliferation, migration, and invasion. Conversely, depletion of miR‐1296‐5p facilitated the growth and mobility of OS cells. Notably, miR‐1296‐5p inversely modulated notch receptor 2 (NOTCH2) in OS cells. The level of NOTCH2 messenger RNA was negatively correlated with miR‐1296‐5p level in OS samples. NOTCH2 knockdown markedly suppressed the abilities of MG‐63 cell proliferation and mobility. More importantly, the restoration of NOTCH2 prominently rescued miR‐1296‐5p‐induced tumor‐suppressive effects on MG‐63 cells. In conclusion, our study identified the reduced expression of miR‐1296‐5p, which contributed to OS progression. miR‐1296‐5p might be a promising prognostic marker and therapeutic target in OS.</jats:p> |
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| author | Wang, Lei, Hu, Kejun, Chao, Yu, Wang, Xueli |
| author_facet | Wang, Lei, Hu, Kejun, Chao, Yu, Wang, Xueli, Wang, Lei, Hu, Kejun, Chao, Yu, Wang, Xueli |
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| description | <jats:title>Abstract</jats:title><jats:p>Osteosarcoma (OS) is a highly aggressive bone tumor with a poor prognosis. MicroRNAs are revealed to exerts essential roles in the carcinogenesis and tumor invasion of OS. But, the function of miR‐1296‐5p and its related mechanism in OS progression have not yet been studied. This study discovered the levels of miR‐1296‐5p in OS and corresponding noncancerous tissues, and we demonstrated that miR‐1296‐5p level was markedly downregulated in tumor specimens as compared with nontumor tissues. In addition, we discovered that miR‐1296‐5p was also underexpressed in OS cells compared with the hFOB1.19 osteoblast cells. Interestingly, the reduced expression of miR‐1296‐5p was confirmed to associated with large tumor size, advanced tumor stages, and distance metastasis, respectively. Patients with OS low‐expressing miR‐1296‐5p showed a prominent shorter survival. In addition, gain‐of‐function assays verified that miR‐1296‐5p overexpression remarkably repressed OS cell proliferation, migration, and invasion. Conversely, depletion of miR‐1296‐5p facilitated the growth and mobility of OS cells. Notably, miR‐1296‐5p inversely modulated notch receptor 2 (NOTCH2) in OS cells. The level of NOTCH2 messenger RNA was negatively correlated with miR‐1296‐5p level in OS samples. NOTCH2 knockdown markedly suppressed the abilities of MG‐63 cell proliferation and mobility. More importantly, the restoration of NOTCH2 prominently rescued miR‐1296‐5p‐induced tumor‐suppressive effects on MG‐63 cells. In conclusion, our study identified the reduced expression of miR‐1296‐5p, which contributed to OS progression. miR‐1296‐5p might be a promising prognostic marker and therapeutic target in OS.</jats:p> |
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| spelling | Wang, Lei Hu, Kejun Chao, Yu Wang, Xueli 0730-2312 1097-4644 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1002/jcb.29438 <jats:title>Abstract</jats:title><jats:p>Osteosarcoma (OS) is a highly aggressive bone tumor with a poor prognosis. MicroRNAs are revealed to exerts essential roles in the carcinogenesis and tumor invasion of OS. But, the function of miR‐1296‐5p and its related mechanism in OS progression have not yet been studied. This study discovered the levels of miR‐1296‐5p in OS and corresponding noncancerous tissues, and we demonstrated that miR‐1296‐5p level was markedly downregulated in tumor specimens as compared with nontumor tissues. In addition, we discovered that miR‐1296‐5p was also underexpressed in OS cells compared with the hFOB1.19 osteoblast cells. Interestingly, the reduced expression of miR‐1296‐5p was confirmed to associated with large tumor size, advanced tumor stages, and distance metastasis, respectively. Patients with OS low‐expressing miR‐1296‐5p showed a prominent shorter survival. In addition, gain‐of‐function assays verified that miR‐1296‐5p overexpression remarkably repressed OS cell proliferation, migration, and invasion. Conversely, depletion of miR‐1296‐5p facilitated the growth and mobility of OS cells. Notably, miR‐1296‐5p inversely modulated notch receptor 2 (NOTCH2) in OS cells. The level of NOTCH2 messenger RNA was negatively correlated with miR‐1296‐5p level in OS samples. NOTCH2 knockdown markedly suppressed the abilities of MG‐63 cell proliferation and mobility. More importantly, the restoration of NOTCH2 prominently rescued miR‐1296‐5p‐induced tumor‐suppressive effects on MG‐63 cells. In conclusion, our study identified the reduced expression of miR‐1296‐5p, which contributed to OS progression. miR‐1296‐5p might be a promising prognostic marker and therapeutic target in OS.</jats:p> MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 Journal of Cellular Biochemistry |
| spellingShingle | Wang, Lei, Hu, Kejun, Chao, Yu, Wang, Xueli, Journal of Cellular Biochemistry, MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2, Cell Biology, Molecular Biology, Biochemistry |
| title | MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 |
| title_full | MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 |
| title_fullStr | MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 |
| title_full_unstemmed | MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 |
| title_short | MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 |
| title_sort | microrna‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting notch2 |
| title_unstemmed | MicroRNA‐1296‐5p suppresses the proliferation, migration, and invasion of human osteosarcoma cells by targeting NOTCH2 |
| topic | Cell Biology, Molecular Biology, Biochemistry |
| url | http://dx.doi.org/10.1002/jcb.29438 |