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Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation
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| Zeitschriftentitel: | Journal of Cellular Biochemistry |
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| Personen und Körperschaften: | , , , , , , , |
| In: | Journal of Cellular Biochemistry, 119, 2018, 2, S. 1879-1888 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Wu, Xiaoqiang Yan, Tianzhong Wang, Zhiwei Wu, Xuan Cao, Guanghui Zhang, Chan Tian, Xiangyong Wang, Junpeng Wu, Xiaoqiang Yan, Tianzhong Wang, Zhiwei Wu, Xuan Cao, Guanghui Zhang, Chan Tian, Xiangyong Wang, Junpeng |
|---|---|
| author |
Wu, Xiaoqiang Yan, Tianzhong Wang, Zhiwei Wu, Xuan Cao, Guanghui Zhang, Chan Tian, Xiangyong Wang, Junpeng |
| spellingShingle |
Wu, Xiaoqiang Yan, Tianzhong Wang, Zhiwei Wu, Xuan Cao, Guanghui Zhang, Chan Tian, Xiangyong Wang, Junpeng Journal of Cellular Biochemistry Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation Cell Biology Molecular Biology Biochemistry |
| author_sort |
wu, xiaoqiang |
| spelling |
Wu, Xiaoqiang Yan, Tianzhong Wang, Zhiwei Wu, Xuan Cao, Guanghui Zhang, Chan Tian, Xiangyong Wang, Junpeng 0730-2312 1097-4644 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1002/jcb.26348 <jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>The purpose of the present study was to investigate the possible therapeutic effects of the human Wharton‐Jelly mesenchymal stromal cells derived micro‐vesicles (hWJMSCs‐MVs) on renal ischemia‐reperfusion injury (IRI) after cardiac death (CD) renal transplantation in rats. MVs were injected intravenously in rats immediately after renal transplantation. The animals were sacrificed at 24 h, 48 h, 1 and 2 weeks post‐transplantation. ELISA was used to determine the von Willebrand Factor (vWF), tumor necrosis factor (TNF)‐α, and interleukin (IL)‐10 levels in the serum. Tubular cell proliferation and apoptosis were identified by Ki67 immunostaining and TUNEL assay. Renal fibrosis was assessed by Masson's tri‐chrome straining and alpha‐smooth muscle actin (α‐SMA) staining. The infiltration of inflammatory cells was detected by CD68<jats:sup>+</jats:sup> staining. The transforming growth factor (TGF)‐β, hepatocyte growth factor (HGF), and α‐SMA expression in the kidney was measured by Western blot. After renal transplantation, the rats treated with hWJMSCs‐MVs improved survival rate and renal function. Moreover, MVs mitigated renal cell apoptosis, enhanced proliferation, and alleviated inflammation at the first 48 h. In the late period, abrogation of renal fibrosis was observed in the MVs group. MVs also could decrease the number of CD68<jats:sup>+</jats:sup> macrophages in the kidney. Furthermore, MVs decreased the protein expression levels of α‐SMA and TGF‐β1 and increased the protein expression level of HGF at any point (24 h, 48 h, 1 or 2 weeks). The administration of MVs immediately after renal transplantation could ameliorate IRI in both the acute and chronic stage.</jats:p></jats:sec> Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation Journal of Cellular Biochemistry |
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Biologie Chemie und Pharmazie |
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| title |
Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| title_unstemmed |
Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| title_full |
Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| title_fullStr |
Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| title_full_unstemmed |
Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| title_short |
Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| title_sort |
micro‐vesicles derived from human wharton's jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| topic |
Cell Biology Molecular Biology Biochemistry |
| url |
http://dx.doi.org/10.1002/jcb.26348 |
| publishDate |
2018 |
| physical |
1879-1888 |
| description |
<jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>The purpose of the present study was to investigate the possible therapeutic effects of the human Wharton‐Jelly mesenchymal stromal cells derived micro‐vesicles (hWJMSCs‐MVs) on renal ischemia‐reperfusion injury (IRI) after cardiac death (CD) renal transplantation in rats. MVs were injected intravenously in rats immediately after renal transplantation. The animals were sacrificed at 24 h, 48 h, 1 and 2 weeks post‐transplantation. ELISA was used to determine the von Willebrand Factor (vWF), tumor necrosis factor (TNF)‐α, and interleukin (IL)‐10 levels in the serum. Tubular cell proliferation and apoptosis were identified by Ki67 immunostaining and TUNEL assay. Renal fibrosis was assessed by Masson's tri‐chrome straining and alpha‐smooth muscle actin (α‐SMA) staining. The infiltration of inflammatory cells was detected by CD68<jats:sup>+</jats:sup> staining. The transforming growth factor (TGF)‐β, hepatocyte growth factor (HGF), and α‐SMA expression in the kidney was measured by Western blot. After renal transplantation, the rats treated with hWJMSCs‐MVs improved survival rate and renal function. Moreover, MVs mitigated renal cell apoptosis, enhanced proliferation, and alleviated inflammation at the first 48 h. In the late period, abrogation of renal fibrosis was observed in the MVs group. MVs also could decrease the number of CD68<jats:sup>+</jats:sup> macrophages in the kidney. Furthermore, MVs decreased the protein expression levels of α‐SMA and TGF‐β1 and increased the protein expression level of HGF at any point (24 h, 48 h, 1 or 2 weeks). The administration of MVs immediately after renal transplantation could ameliorate IRI in both the acute and chronic stage.</jats:p></jats:sec> |
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| author | Wu, Xiaoqiang, Yan, Tianzhong, Wang, Zhiwei, Wu, Xuan, Cao, Guanghui, Zhang, Chan, Tian, Xiangyong, Wang, Junpeng |
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| description | <jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>The purpose of the present study was to investigate the possible therapeutic effects of the human Wharton‐Jelly mesenchymal stromal cells derived micro‐vesicles (hWJMSCs‐MVs) on renal ischemia‐reperfusion injury (IRI) after cardiac death (CD) renal transplantation in rats. MVs were injected intravenously in rats immediately after renal transplantation. The animals were sacrificed at 24 h, 48 h, 1 and 2 weeks post‐transplantation. ELISA was used to determine the von Willebrand Factor (vWF), tumor necrosis factor (TNF)‐α, and interleukin (IL)‐10 levels in the serum. Tubular cell proliferation and apoptosis were identified by Ki67 immunostaining and TUNEL assay. Renal fibrosis was assessed by Masson's tri‐chrome straining and alpha‐smooth muscle actin (α‐SMA) staining. The infiltration of inflammatory cells was detected by CD68<jats:sup>+</jats:sup> staining. The transforming growth factor (TGF)‐β, hepatocyte growth factor (HGF), and α‐SMA expression in the kidney was measured by Western blot. After renal transplantation, the rats treated with hWJMSCs‐MVs improved survival rate and renal function. Moreover, MVs mitigated renal cell apoptosis, enhanced proliferation, and alleviated inflammation at the first 48 h. In the late period, abrogation of renal fibrosis was observed in the MVs group. MVs also could decrease the number of CD68<jats:sup>+</jats:sup> macrophages in the kidney. Furthermore, MVs decreased the protein expression levels of α‐SMA and TGF‐β1 and increased the protein expression level of HGF at any point (24 h, 48 h, 1 or 2 weeks). The administration of MVs immediately after renal transplantation could ameliorate IRI in both the acute and chronic stage.</jats:p></jats:sec> |
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| institution | DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1 |
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| spelling | Wu, Xiaoqiang Yan, Tianzhong Wang, Zhiwei Wu, Xuan Cao, Guanghui Zhang, Chan Tian, Xiangyong Wang, Junpeng 0730-2312 1097-4644 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1002/jcb.26348 <jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>The purpose of the present study was to investigate the possible therapeutic effects of the human Wharton‐Jelly mesenchymal stromal cells derived micro‐vesicles (hWJMSCs‐MVs) on renal ischemia‐reperfusion injury (IRI) after cardiac death (CD) renal transplantation in rats. MVs were injected intravenously in rats immediately after renal transplantation. The animals were sacrificed at 24 h, 48 h, 1 and 2 weeks post‐transplantation. ELISA was used to determine the von Willebrand Factor (vWF), tumor necrosis factor (TNF)‐α, and interleukin (IL)‐10 levels in the serum. Tubular cell proliferation and apoptosis were identified by Ki67 immunostaining and TUNEL assay. Renal fibrosis was assessed by Masson's tri‐chrome straining and alpha‐smooth muscle actin (α‐SMA) staining. The infiltration of inflammatory cells was detected by CD68<jats:sup>+</jats:sup> staining. The transforming growth factor (TGF)‐β, hepatocyte growth factor (HGF), and α‐SMA expression in the kidney was measured by Western blot. After renal transplantation, the rats treated with hWJMSCs‐MVs improved survival rate and renal function. Moreover, MVs mitigated renal cell apoptosis, enhanced proliferation, and alleviated inflammation at the first 48 h. In the late period, abrogation of renal fibrosis was observed in the MVs group. MVs also could decrease the number of CD68<jats:sup>+</jats:sup> macrophages in the kidney. Furthermore, MVs decreased the protein expression levels of α‐SMA and TGF‐β1 and increased the protein expression level of HGF at any point (24 h, 48 h, 1 or 2 weeks). The administration of MVs immediately after renal transplantation could ameliorate IRI in both the acute and chronic stage.</jats:p></jats:sec> Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation Journal of Cellular Biochemistry |
| spellingShingle | Wu, Xiaoqiang, Yan, Tianzhong, Wang, Zhiwei, Wu, Xuan, Cao, Guanghui, Zhang, Chan, Tian, Xiangyong, Wang, Junpeng, Journal of Cellular Biochemistry, Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation, Cell Biology, Molecular Biology, Biochemistry |
| title | Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| title_full | Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| title_fullStr | Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| title_full_unstemmed | Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| title_short | Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| title_sort | micro‐vesicles derived from human wharton's jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| title_unstemmed | Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation |
| topic | Cell Biology, Molecular Biology, Biochemistry |
| url | http://dx.doi.org/10.1002/jcb.26348 |