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Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer
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| Zeitschriftentitel: | International Journal of Cancer |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , |
| In: | International Journal of Cancer, 132, 2013, 9, S. 2107-2117 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Kumagai, Hironori Pham, Wellington Kataoka, Makoto Hiwatari, Ken‐Ichiro McBride, James Wilson, Kevin J. Tachikawa, Hiroyuki Kimura, Ryoji Nakamura, Kunio Liu, Eric H. Gore, John C. Sakuma, Shinji Kumagai, Hironori Pham, Wellington Kataoka, Makoto Hiwatari, Ken‐Ichiro McBride, James Wilson, Kevin J. Tachikawa, Hiroyuki Kimura, Ryoji Nakamura, Kunio Liu, Eric H. Gore, John C. Sakuma, Shinji |
|---|---|
| author |
Kumagai, Hironori Pham, Wellington Kataoka, Makoto Hiwatari, Ken‐Ichiro McBride, James Wilson, Kevin J. Tachikawa, Hiroyuki Kimura, Ryoji Nakamura, Kunio Liu, Eric H. Gore, John C. Sakuma, Shinji |
| spellingShingle |
Kumagai, Hironori Pham, Wellington Kataoka, Makoto Hiwatari, Ken‐Ichiro McBride, James Wilson, Kevin J. Tachikawa, Hiroyuki Kimura, Ryoji Nakamura, Kunio Liu, Eric H. Gore, John C. Sakuma, Shinji International Journal of Cancer Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer Cancer Research Oncology |
| author_sort |
kumagai, hironori |
| spelling |
Kumagai, Hironori Pham, Wellington Kataoka, Makoto Hiwatari, Ken‐Ichiro McBride, James Wilson, Kevin J. Tachikawa, Hiroyuki Kimura, Ryoji Nakamura, Kunio Liu, Eric H. Gore, John C. Sakuma, Shinji 0020-7136 1097-0215 Wiley Cancer Research Oncology http://dx.doi.org/10.1002/ijc.27903 <jats:title>Abstract</jats:title><jats:p>This research aimed to validate the specificity of the newly developed nanobeacon for imaging the Thomsen‐Friedenreich (TF) antigen, a potential biomarker of colorectal cancer. The imaging agent is comprised of a submicron‐sized polystyrene nanosphere encapsulated with a Coumarin 6 dye. The surface of the nanosphere was modified with peanut agglutinin (PNA) and poly(<jats:italic>N</jats:italic>‐vinylacetamide (PNVA) moieties. The former binds to Gal‐β(1‐3)GalNAc with high affinity while the latter enhances the specificity of PNA for the carbohydrates. The specificity of the nanobeacon was evaluated in human colorectal cancer cells and specimens, and the data were compared with immunohistochemical staining and flow cytometric analysis. Additionally, distribution of the nanobeacon <jats:italic>in vivo</jats:italic> was assessed using an “intestinal loop” mouse model. Quantitative analysis of the data indicated that approximately 2 μg of PNA were detected for each milligram of the nanobeacon. The nanobeacon specifically reported colorectal tumors by recognizing the tumor‐specific antigen through the surface‐immobilized PNA. Removal of TF from human colorectal cancer cells and tissues resulted in a loss of fluorescence signal, which suggests the specificity of the probe. Most importantly, the probe was not absorbed systematically in the large intestine upon topical application. As a result, no registered toxicity was associated with the probe. These data demonstrate the potential use of this novel nanobeacon for imaging the TF antigen as a biomarker for the early detection and prediction of the progression of colorectal cancer at the molecular level.</jats:p> Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer International Journal of Cancer |
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| title |
Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer |
| title_unstemmed |
Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer |
| title_full |
Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer |
| title_fullStr |
Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer |
| title_full_unstemmed |
Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer |
| title_short |
Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer |
| title_sort |
multifunctional nanobeacon for imaging thomsen‐friedenreich antigen‐associated colorectal cancer |
| topic |
Cancer Research Oncology |
| url |
http://dx.doi.org/10.1002/ijc.27903 |
| publishDate |
2013 |
| physical |
2107-2117 |
| description |
<jats:title>Abstract</jats:title><jats:p>This research aimed to validate the specificity of the newly developed nanobeacon for imaging the Thomsen‐Friedenreich (TF) antigen, a potential biomarker of colorectal cancer. The imaging agent is comprised of a submicron‐sized polystyrene nanosphere encapsulated with a Coumarin 6 dye. The surface of the nanosphere was modified with peanut agglutinin (PNA) and poly(<jats:italic>N</jats:italic>‐vinylacetamide (PNVA) moieties. The former binds to Gal‐β(1‐3)GalNAc with high affinity while the latter enhances the specificity of PNA for the carbohydrates. The specificity of the nanobeacon was evaluated in human colorectal cancer cells and specimens, and the data were compared with immunohistochemical staining and flow cytometric analysis. Additionally, distribution of the nanobeacon <jats:italic>in vivo</jats:italic> was assessed using an “intestinal loop” mouse model. Quantitative analysis of the data indicated that approximately 2 μg of PNA were detected for each milligram of the nanobeacon. The nanobeacon specifically reported colorectal tumors by recognizing the tumor‐specific antigen through the surface‐immobilized PNA. Removal of TF from human colorectal cancer cells and tissues resulted in a loss of fluorescence signal, which suggests the specificity of the probe. Most importantly, the probe was not absorbed systematically in the large intestine upon topical application. As a result, no registered toxicity was associated with the probe. These data demonstrate the potential use of this novel nanobeacon for imaging the TF antigen as a biomarker for the early detection and prediction of the progression of colorectal cancer at the molecular level.</jats:p> |
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| author | Kumagai, Hironori, Pham, Wellington, Kataoka, Makoto, Hiwatari, Ken‐Ichiro, McBride, James, Wilson, Kevin J., Tachikawa, Hiroyuki, Kimura, Ryoji, Nakamura, Kunio, Liu, Eric H., Gore, John C., Sakuma, Shinji |
| author_facet | Kumagai, Hironori, Pham, Wellington, Kataoka, Makoto, Hiwatari, Ken‐Ichiro, McBride, James, Wilson, Kevin J., Tachikawa, Hiroyuki, Kimura, Ryoji, Nakamura, Kunio, Liu, Eric H., Gore, John C., Sakuma, Shinji, Kumagai, Hironori, Pham, Wellington, Kataoka, Makoto, Hiwatari, Ken‐Ichiro, McBride, James, Wilson, Kevin J., Tachikawa, Hiroyuki, Kimura, Ryoji, Nakamura, Kunio, Liu, Eric H., Gore, John C., Sakuma, Shinji |
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| container_issue | 9 |
| container_start_page | 2107 |
| container_title | International Journal of Cancer |
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| description | <jats:title>Abstract</jats:title><jats:p>This research aimed to validate the specificity of the newly developed nanobeacon for imaging the Thomsen‐Friedenreich (TF) antigen, a potential biomarker of colorectal cancer. The imaging agent is comprised of a submicron‐sized polystyrene nanosphere encapsulated with a Coumarin 6 dye. The surface of the nanosphere was modified with peanut agglutinin (PNA) and poly(<jats:italic>N</jats:italic>‐vinylacetamide (PNVA) moieties. The former binds to Gal‐β(1‐3)GalNAc with high affinity while the latter enhances the specificity of PNA for the carbohydrates. The specificity of the nanobeacon was evaluated in human colorectal cancer cells and specimens, and the data were compared with immunohistochemical staining and flow cytometric analysis. Additionally, distribution of the nanobeacon <jats:italic>in vivo</jats:italic> was assessed using an “intestinal loop” mouse model. Quantitative analysis of the data indicated that approximately 2 μg of PNA were detected for each milligram of the nanobeacon. The nanobeacon specifically reported colorectal tumors by recognizing the tumor‐specific antigen through the surface‐immobilized PNA. Removal of TF from human colorectal cancer cells and tissues resulted in a loss of fluorescence signal, which suggests the specificity of the probe. Most importantly, the probe was not absorbed systematically in the large intestine upon topical application. As a result, no registered toxicity was associated with the probe. These data demonstrate the potential use of this novel nanobeacon for imaging the TF antigen as a biomarker for the early detection and prediction of the progression of colorectal cancer at the molecular level.</jats:p> |
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| spelling | Kumagai, Hironori Pham, Wellington Kataoka, Makoto Hiwatari, Ken‐Ichiro McBride, James Wilson, Kevin J. Tachikawa, Hiroyuki Kimura, Ryoji Nakamura, Kunio Liu, Eric H. Gore, John C. Sakuma, Shinji 0020-7136 1097-0215 Wiley Cancer Research Oncology http://dx.doi.org/10.1002/ijc.27903 <jats:title>Abstract</jats:title><jats:p>This research aimed to validate the specificity of the newly developed nanobeacon for imaging the Thomsen‐Friedenreich (TF) antigen, a potential biomarker of colorectal cancer. The imaging agent is comprised of a submicron‐sized polystyrene nanosphere encapsulated with a Coumarin 6 dye. The surface of the nanosphere was modified with peanut agglutinin (PNA) and poly(<jats:italic>N</jats:italic>‐vinylacetamide (PNVA) moieties. The former binds to Gal‐β(1‐3)GalNAc with high affinity while the latter enhances the specificity of PNA for the carbohydrates. The specificity of the nanobeacon was evaluated in human colorectal cancer cells and specimens, and the data were compared with immunohistochemical staining and flow cytometric analysis. Additionally, distribution of the nanobeacon <jats:italic>in vivo</jats:italic> was assessed using an “intestinal loop” mouse model. Quantitative analysis of the data indicated that approximately 2 μg of PNA were detected for each milligram of the nanobeacon. The nanobeacon specifically reported colorectal tumors by recognizing the tumor‐specific antigen through the surface‐immobilized PNA. Removal of TF from human colorectal cancer cells and tissues resulted in a loss of fluorescence signal, which suggests the specificity of the probe. Most importantly, the probe was not absorbed systematically in the large intestine upon topical application. As a result, no registered toxicity was associated with the probe. These data demonstrate the potential use of this novel nanobeacon for imaging the TF antigen as a biomarker for the early detection and prediction of the progression of colorectal cancer at the molecular level.</jats:p> Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer International Journal of Cancer |
| spellingShingle | Kumagai, Hironori, Pham, Wellington, Kataoka, Makoto, Hiwatari, Ken‐Ichiro, McBride, James, Wilson, Kevin J., Tachikawa, Hiroyuki, Kimura, Ryoji, Nakamura, Kunio, Liu, Eric H., Gore, John C., Sakuma, Shinji, International Journal of Cancer, Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer, Cancer Research, Oncology |
| title | Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer |
| title_full | Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer |
| title_fullStr | Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer |
| title_full_unstemmed | Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer |
| title_short | Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer |
| title_sort | multifunctional nanobeacon for imaging thomsen‐friedenreich antigen‐associated colorectal cancer |
| title_unstemmed | Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer |
| topic | Cancer Research, Oncology |
| url | http://dx.doi.org/10.1002/ijc.27903 |