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Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line
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| Zeitschriftentitel: | International Journal of Cancer |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | International Journal of Cancer, 132, 2013, 8, S. 1954-1958 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Stürzl, Michael Gaus, Dominika Dirks, Wilhelm G. Ganem, Don Jochmann, Ramona Stürzl, Michael Gaus, Dominika Dirks, Wilhelm G. Ganem, Don Jochmann, Ramona |
|---|---|
| author |
Stürzl, Michael Gaus, Dominika Dirks, Wilhelm G. Ganem, Don Jochmann, Ramona |
| spellingShingle |
Stürzl, Michael Gaus, Dominika Dirks, Wilhelm G. Ganem, Don Jochmann, Ramona International Journal of Cancer Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line Cancer Research Oncology |
| author_sort |
stürzl, michael |
| spelling |
Stürzl, Michael Gaus, Dominika Dirks, Wilhelm G. Ganem, Don Jochmann, Ramona 0020-7136 1097-0215 Wiley Cancer Research Oncology http://dx.doi.org/10.1002/ijc.27849 <jats:title>Abstract</jats:title><jats:p>Kaposi's sarcoma (KS) is an endothelial cell‐derived tumor. Investigations of the molecular mechanisms of KS pathogenesis and the identification of drugs for treatment of KS depend critically on valid cell‐culture models. Two major immortalized cell lines are available for KS research. Recently, the KS cell line KS Y‐1 has been shown to be cross‐contaminated with the T24 urinary bladder cancer cell line (ATCC HTB‐4). Here, we show by short tandem repeat profiling that the second KS cell line, SLK, is indistinguishable from the clear‐cell renal‐cell carcinoma cell line Caki‐1. Immunocytochemical detection of cytokeratin expression confirmed the epithelial‐cell origin of SLK cells. Our findings indicate that SLK cells are not of endothelial origin and should not be used in future studies as a model for KS‐derived endothelial tumor cells. We suggest that in the future, more attention needs to be paid to the authenticity of cells in lines derived from human tissues.</jats:p> Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line International Journal of Cancer |
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Wiley, 2013 |
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International Journal of Cancer |
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| title |
Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| title_unstemmed |
Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| title_full |
Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| title_fullStr |
Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| title_full_unstemmed |
Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| title_short |
Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| title_sort |
kaposi's sarcoma‐derived cell line slk is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| topic |
Cancer Research Oncology |
| url |
http://dx.doi.org/10.1002/ijc.27849 |
| publishDate |
2013 |
| physical |
1954-1958 |
| description |
<jats:title>Abstract</jats:title><jats:p>Kaposi's sarcoma (KS) is an endothelial cell‐derived tumor. Investigations of the molecular mechanisms of KS pathogenesis and the identification of drugs for treatment of KS depend critically on valid cell‐culture models. Two major immortalized cell lines are available for KS research. Recently, the KS cell line KS Y‐1 has been shown to be cross‐contaminated with the T24 urinary bladder cancer cell line (ATCC HTB‐4). Here, we show by short tandem repeat profiling that the second KS cell line, SLK, is indistinguishable from the clear‐cell renal‐cell carcinoma cell line Caki‐1. Immunocytochemical detection of cytokeratin expression confirmed the epithelial‐cell origin of SLK cells. Our findings indicate that SLK cells are not of endothelial origin and should not be used in future studies as a model for KS‐derived endothelial tumor cells. We suggest that in the future, more attention needs to be paid to the authenticity of cells in lines derived from human tissues.</jats:p> |
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| author | Stürzl, Michael, Gaus, Dominika, Dirks, Wilhelm G., Ganem, Don, Jochmann, Ramona |
| author_facet | Stürzl, Michael, Gaus, Dominika, Dirks, Wilhelm G., Ganem, Don, Jochmann, Ramona, Stürzl, Michael, Gaus, Dominika, Dirks, Wilhelm G., Ganem, Don, Jochmann, Ramona |
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| container_title | International Journal of Cancer |
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| description | <jats:title>Abstract</jats:title><jats:p>Kaposi's sarcoma (KS) is an endothelial cell‐derived tumor. Investigations of the molecular mechanisms of KS pathogenesis and the identification of drugs for treatment of KS depend critically on valid cell‐culture models. Two major immortalized cell lines are available for KS research. Recently, the KS cell line KS Y‐1 has been shown to be cross‐contaminated with the T24 urinary bladder cancer cell line (ATCC HTB‐4). Here, we show by short tandem repeat profiling that the second KS cell line, SLK, is indistinguishable from the clear‐cell renal‐cell carcinoma cell line Caki‐1. Immunocytochemical detection of cytokeratin expression confirmed the epithelial‐cell origin of SLK cells. Our findings indicate that SLK cells are not of endothelial origin and should not be used in future studies as a model for KS‐derived endothelial tumor cells. We suggest that in the future, more attention needs to be paid to the authenticity of cells in lines derived from human tissues.</jats:p> |
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| spelling | Stürzl, Michael Gaus, Dominika Dirks, Wilhelm G. Ganem, Don Jochmann, Ramona 0020-7136 1097-0215 Wiley Cancer Research Oncology http://dx.doi.org/10.1002/ijc.27849 <jats:title>Abstract</jats:title><jats:p>Kaposi's sarcoma (KS) is an endothelial cell‐derived tumor. Investigations of the molecular mechanisms of KS pathogenesis and the identification of drugs for treatment of KS depend critically on valid cell‐culture models. Two major immortalized cell lines are available for KS research. Recently, the KS cell line KS Y‐1 has been shown to be cross‐contaminated with the T24 urinary bladder cancer cell line (ATCC HTB‐4). Here, we show by short tandem repeat profiling that the second KS cell line, SLK, is indistinguishable from the clear‐cell renal‐cell carcinoma cell line Caki‐1. Immunocytochemical detection of cytokeratin expression confirmed the epithelial‐cell origin of SLK cells. Our findings indicate that SLK cells are not of endothelial origin and should not be used in future studies as a model for KS‐derived endothelial tumor cells. We suggest that in the future, more attention needs to be paid to the authenticity of cells in lines derived from human tissues.</jats:p> Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line International Journal of Cancer |
| spellingShingle | Stürzl, Michael, Gaus, Dominika, Dirks, Wilhelm G., Ganem, Don, Jochmann, Ramona, International Journal of Cancer, Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line, Cancer Research, Oncology |
| title | Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| title_full | Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| title_fullStr | Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| title_full_unstemmed | Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| title_short | Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| title_sort | kaposi's sarcoma‐derived cell line slk is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| title_unstemmed | Kaposi's sarcoma‐derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line |
| topic | Cancer Research, Oncology |
| url | http://dx.doi.org/10.1002/ijc.27849 |