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Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–pr...
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| Zeitschriftentitel: | ELECTROPHORESIS |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | ELECTROPHORESIS, 36, 2015, 21-22, S. 2811-2819 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Kotz, Sandra Kullmann, Maximilian Crone, Barbara Kalayda, Ganna V. Jaehde, Ulrich Metzger, Sabine Kotz, Sandra Kullmann, Maximilian Crone, Barbara Kalayda, Ganna V. Jaehde, Ulrich Metzger, Sabine |
|---|---|
| author |
Kotz, Sandra Kullmann, Maximilian Crone, Barbara Kalayda, Ganna V. Jaehde, Ulrich Metzger, Sabine |
| spellingShingle |
Kotz, Sandra Kullmann, Maximilian Crone, Barbara Kalayda, Ganna V. Jaehde, Ulrich Metzger, Sabine ELECTROPHORESIS Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts Clinical Biochemistry Biochemistry Analytical Chemistry |
| author_sort |
kotz, sandra |
| spelling |
Kotz, Sandra Kullmann, Maximilian Crone, Barbara Kalayda, Ganna V. Jaehde, Ulrich Metzger, Sabine 0173-0835 1522-2683 Wiley Clinical Biochemistry Biochemistry Analytical Chemistry http://dx.doi.org/10.1002/elps.201500188 <jats:p>Cisplatin is one of the most widely used anticancer agents, but a major problem for successful chemotherapy is the development of drug resistance of tumor cells against cisplatin. Resistance to cisplatin is a multifactorial problem. A method to detect and identify intracellular cisplatin–protein adducts was developed using a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue (CFDA–cisplatin), 2DE, and ESI‐MS/MS. We identified several CFDA–cisplatin–protein adducts including members of the protein disulfide isomerase family (PDI). These are the first results of the detection of intracellular CFDA–cisplatin–protein adducts, which may help to understand the resistance mechanism of cisplatin.</jats:p> Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts ELECTROPHORESIS |
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Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| title_unstemmed |
Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| title_full |
Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| title_fullStr |
Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| title_full_unstemmed |
Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| title_short |
Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| title_sort |
combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| topic |
Clinical Biochemistry Biochemistry Analytical Chemistry |
| url |
http://dx.doi.org/10.1002/elps.201500188 |
| publishDate |
2015 |
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2811-2819 |
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<jats:p>Cisplatin is one of the most widely used anticancer agents, but a major problem for successful chemotherapy is the development of drug resistance of tumor cells against cisplatin. Resistance to cisplatin is a multifactorial problem. A method to detect and identify intracellular cisplatin–protein adducts was developed using a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue (CFDA–cisplatin), 2DE, and ESI‐MS/MS. We identified several CFDA–cisplatin–protein adducts including members of the protein disulfide isomerase family (PDI). These are the first results of the detection of intracellular CFDA–cisplatin–protein adducts, which may help to understand the resistance mechanism of cisplatin.</jats:p> |
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| author_facet | Kotz, Sandra, Kullmann, Maximilian, Crone, Barbara, Kalayda, Ganna V., Jaehde, Ulrich, Metzger, Sabine, Kotz, Sandra, Kullmann, Maximilian, Crone, Barbara, Kalayda, Ganna V., Jaehde, Ulrich, Metzger, Sabine |
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| description | <jats:p>Cisplatin is one of the most widely used anticancer agents, but a major problem for successful chemotherapy is the development of drug resistance of tumor cells against cisplatin. Resistance to cisplatin is a multifactorial problem. A method to detect and identify intracellular cisplatin–protein adducts was developed using a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue (CFDA–cisplatin), 2DE, and ESI‐MS/MS. We identified several CFDA–cisplatin–protein adducts including members of the protein disulfide isomerase family (PDI). These are the first results of the detection of intracellular CFDA–cisplatin–protein adducts, which may help to understand the resistance mechanism of cisplatin.</jats:p> |
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| spelling | Kotz, Sandra Kullmann, Maximilian Crone, Barbara Kalayda, Ganna V. Jaehde, Ulrich Metzger, Sabine 0173-0835 1522-2683 Wiley Clinical Biochemistry Biochemistry Analytical Chemistry http://dx.doi.org/10.1002/elps.201500188 <jats:p>Cisplatin is one of the most widely used anticancer agents, but a major problem for successful chemotherapy is the development of drug resistance of tumor cells against cisplatin. Resistance to cisplatin is a multifactorial problem. A method to detect and identify intracellular cisplatin–protein adducts was developed using a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue (CFDA–cisplatin), 2DE, and ESI‐MS/MS. We identified several CFDA–cisplatin–protein adducts including members of the protein disulfide isomerase family (PDI). These are the first results of the detection of intracellular CFDA–cisplatin–protein adducts, which may help to understand the resistance mechanism of cisplatin.</jats:p> Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts ELECTROPHORESIS |
| spellingShingle | Kotz, Sandra, Kullmann, Maximilian, Crone, Barbara, Kalayda, Ganna V., Jaehde, Ulrich, Metzger, Sabine, ELECTROPHORESIS, Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts, Clinical Biochemistry, Biochemistry, Analytical Chemistry |
| title | Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| title_full | Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| title_fullStr | Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| title_full_unstemmed | Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| title_short | Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| title_sort | combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| title_unstemmed | Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts |
| topic | Clinical Biochemistry, Biochemistry, Analytical Chemistry |
| url | http://dx.doi.org/10.1002/elps.201500188 |