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Purity of glycosaminoglycan‐related compounds using capillary electrophoresis
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| Zeitschriftentitel: | ELECTROPHORESIS |
|---|---|
| Personen und Körperschaften: | , |
| In: | ELECTROPHORESIS, 17, 1996, 2, S. 401-405 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Malsch, Reinhard Harenberg, Job Malsch, Reinhard Harenberg, Job |
|---|---|
| author |
Malsch, Reinhard Harenberg, Job |
| spellingShingle |
Malsch, Reinhard Harenberg, Job ELECTROPHORESIS Purity of glycosaminoglycan‐related compounds using capillary electrophoresis Clinical Biochemistry Biochemistry Analytical Chemistry |
| author_sort |
malsch, reinhard |
| spelling |
Malsch, Reinhard Harenberg, Job 0173-0835 1522-2683 Wiley Clinical Biochemistry Biochemistry Analytical Chemistry http://dx.doi.org/10.1002/elps.1150170219 <jats:title>Abstract</jats:title><jats:p>High performance capillary electrophoresis was used to determine impurities in glycosaminoglycans. The counterion of glycosaminoglycans was analyzed with indirect UV‐detection using a 40 m<jats:sc>M</jats:sc> 4‐aminopyridine buffer. Calcium, lithium, potassium and sodium could be resolved. A linear correlation between the area under the curve and the concentration of sodium (<jats:italic>r</jats:italic><jats:sup>2</jats:sup> = 0.98) and calcium (<jats:italic>r</jats:italic><jats:sup>2</jats:sup> = 0.99) was found. Using enzymatic depolymerization, chondroitin sulfates were cleaved to disaccharides. The resulting disaccharides, with the structure 4‐deoxy‐α‐L‐threo‐hex‐4‐enopyranosyl uronic acid (ΔUA) 2 × (1 → 3)‐D‐GalNY6X (X = H, sulfate and Y = acetyl, sulfate) for dermatan sulfate, were detected selectively at 230 nm using capillary electrophoresis. Dermatan sulfate disaccharides were analyzed using a 50 cm long fused silica capillary (75 μm ID). The buffer used was 10 m<jats:sc>M</jats:sc> sodium tetraborate and 50 m<jats:sc>M</jats:sc> SDS, pH 8.8. The detection was at 230 nm. Using the main peak ΔUA (1 → 3)‐D‐GalNAc4S as standard, between 1 and 80% dermatan sulfate in heparin preparations were analyzed. The disaccharide showed a linear correlation of the peak area <jats:italic>versus</jats:italic> the concentration with a correlation coefficient <jats:italic>r</jats:italic><jats:sup>2</jats:sup> = 0.98. The methods are useful in characterizing the identity and concentration of the counterion of glycosaminoglycans after chondroitinase degradation.</jats:p> Purity of glycosaminoglycan‐related compounds using capillary electrophoresis ELECTROPHORESIS |
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1996 |
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ELECTROPHORESIS |
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| title |
Purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| title_unstemmed |
Purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| title_full |
Purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| title_fullStr |
Purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| title_full_unstemmed |
Purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| title_short |
Purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| title_sort |
purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| topic |
Clinical Biochemistry Biochemistry Analytical Chemistry |
| url |
http://dx.doi.org/10.1002/elps.1150170219 |
| publishDate |
1996 |
| physical |
401-405 |
| description |
<jats:title>Abstract</jats:title><jats:p>High performance capillary electrophoresis was used to determine impurities in glycosaminoglycans. The counterion of glycosaminoglycans was analyzed with indirect UV‐detection using a 40 m<jats:sc>M</jats:sc> 4‐aminopyridine buffer. Calcium, lithium, potassium and sodium could be resolved. A linear correlation between the area under the curve and the concentration of sodium (<jats:italic>r</jats:italic><jats:sup>2</jats:sup> = 0.98) and calcium (<jats:italic>r</jats:italic><jats:sup>2</jats:sup> = 0.99) was found. Using enzymatic depolymerization, chondroitin sulfates were cleaved to disaccharides. The resulting disaccharides, with the structure 4‐deoxy‐α‐L‐threo‐hex‐4‐enopyranosyl uronic acid (ΔUA) 2 × (1 → 3)‐D‐GalNY6X (X = H, sulfate and Y = acetyl, sulfate) for dermatan sulfate, were detected selectively at 230 nm using capillary electrophoresis. Dermatan sulfate disaccharides were analyzed using a 50 cm long fused silica capillary (75 μm ID). The buffer used was 10 m<jats:sc>M</jats:sc> sodium tetraborate and 50 m<jats:sc>M</jats:sc> SDS, pH 8.8. The detection was at 230 nm. Using the main peak ΔUA (1 → 3)‐D‐GalNAc4S as standard, between 1 and 80% dermatan sulfate in heparin preparations were analyzed. The disaccharide showed a linear correlation of the peak area <jats:italic>versus</jats:italic> the concentration with a correlation coefficient <jats:italic>r</jats:italic><jats:sup>2</jats:sup> = 0.98. The methods are useful in characterizing the identity and concentration of the counterion of glycosaminoglycans after chondroitinase degradation.</jats:p> |
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| author | Malsch, Reinhard, Harenberg, Job |
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| description | <jats:title>Abstract</jats:title><jats:p>High performance capillary electrophoresis was used to determine impurities in glycosaminoglycans. The counterion of glycosaminoglycans was analyzed with indirect UV‐detection using a 40 m<jats:sc>M</jats:sc> 4‐aminopyridine buffer. Calcium, lithium, potassium and sodium could be resolved. A linear correlation between the area under the curve and the concentration of sodium (<jats:italic>r</jats:italic><jats:sup>2</jats:sup> = 0.98) and calcium (<jats:italic>r</jats:italic><jats:sup>2</jats:sup> = 0.99) was found. Using enzymatic depolymerization, chondroitin sulfates were cleaved to disaccharides. The resulting disaccharides, with the structure 4‐deoxy‐α‐L‐threo‐hex‐4‐enopyranosyl uronic acid (ΔUA) 2 × (1 → 3)‐D‐GalNY6X (X = H, sulfate and Y = acetyl, sulfate) for dermatan sulfate, were detected selectively at 230 nm using capillary electrophoresis. Dermatan sulfate disaccharides were analyzed using a 50 cm long fused silica capillary (75 μm ID). The buffer used was 10 m<jats:sc>M</jats:sc> sodium tetraborate and 50 m<jats:sc>M</jats:sc> SDS, pH 8.8. The detection was at 230 nm. Using the main peak ΔUA (1 → 3)‐D‐GalNAc4S as standard, between 1 and 80% dermatan sulfate in heparin preparations were analyzed. The disaccharide showed a linear correlation of the peak area <jats:italic>versus</jats:italic> the concentration with a correlation coefficient <jats:italic>r</jats:italic><jats:sup>2</jats:sup> = 0.98. The methods are useful in characterizing the identity and concentration of the counterion of glycosaminoglycans after chondroitinase degradation.</jats:p> |
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| spelling | Malsch, Reinhard Harenberg, Job 0173-0835 1522-2683 Wiley Clinical Biochemistry Biochemistry Analytical Chemistry http://dx.doi.org/10.1002/elps.1150170219 <jats:title>Abstract</jats:title><jats:p>High performance capillary electrophoresis was used to determine impurities in glycosaminoglycans. The counterion of glycosaminoglycans was analyzed with indirect UV‐detection using a 40 m<jats:sc>M</jats:sc> 4‐aminopyridine buffer. Calcium, lithium, potassium and sodium could be resolved. A linear correlation between the area under the curve and the concentration of sodium (<jats:italic>r</jats:italic><jats:sup>2</jats:sup> = 0.98) and calcium (<jats:italic>r</jats:italic><jats:sup>2</jats:sup> = 0.99) was found. Using enzymatic depolymerization, chondroitin sulfates were cleaved to disaccharides. The resulting disaccharides, with the structure 4‐deoxy‐α‐L‐threo‐hex‐4‐enopyranosyl uronic acid (ΔUA) 2 × (1 → 3)‐D‐GalNY6X (X = H, sulfate and Y = acetyl, sulfate) for dermatan sulfate, were detected selectively at 230 nm using capillary electrophoresis. Dermatan sulfate disaccharides were analyzed using a 50 cm long fused silica capillary (75 μm ID). The buffer used was 10 m<jats:sc>M</jats:sc> sodium tetraborate and 50 m<jats:sc>M</jats:sc> SDS, pH 8.8. The detection was at 230 nm. Using the main peak ΔUA (1 → 3)‐D‐GalNAc4S as standard, between 1 and 80% dermatan sulfate in heparin preparations were analyzed. The disaccharide showed a linear correlation of the peak area <jats:italic>versus</jats:italic> the concentration with a correlation coefficient <jats:italic>r</jats:italic><jats:sup>2</jats:sup> = 0.98. The methods are useful in characterizing the identity and concentration of the counterion of glycosaminoglycans after chondroitinase degradation.</jats:p> Purity of glycosaminoglycan‐related compounds using capillary electrophoresis ELECTROPHORESIS |
| spellingShingle | Malsch, Reinhard, Harenberg, Job, ELECTROPHORESIS, Purity of glycosaminoglycan‐related compounds using capillary electrophoresis, Clinical Biochemistry, Biochemistry, Analytical Chemistry |
| title | Purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| title_full | Purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| title_fullStr | Purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| title_full_unstemmed | Purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| title_short | Purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| title_sort | purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| title_unstemmed | Purity of glycosaminoglycan‐related compounds using capillary electrophoresis |
| topic | Clinical Biochemistry, Biochemistry, Analytical Chemistry |
| url | http://dx.doi.org/10.1002/elps.1150170219 |