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On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe
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| Zeitschriftentitel: | Electroanalysis |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Electroanalysis, 9, 1997, 12, S. 912-916 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Horiuchi, Tsutomu Torimitsu, Keiichi Yamamoto, Katsunobu Niwa, Osamu Horiuchi, Tsutomu Torimitsu, Keiichi Yamamoto, Katsunobu Niwa, Osamu |
|---|---|
| author |
Horiuchi, Tsutomu Torimitsu, Keiichi Yamamoto, Katsunobu Niwa, Osamu |
| spellingShingle |
Horiuchi, Tsutomu Torimitsu, Keiichi Yamamoto, Katsunobu Niwa, Osamu Electroanalysis On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe Electrochemistry Analytical Chemistry |
| author_sort |
horiuchi, tsutomu |
| spelling |
Horiuchi, Tsutomu Torimitsu, Keiichi Yamamoto, Katsunobu Niwa, Osamu 1040-0397 1521-4109 Wiley Electrochemistry Analytical Chemistry http://dx.doi.org/10.1002/elan.1140091209 <jats:title>Abstract</jats:title><jats:p>We have developed an on‐line electrochemical sensor for the continuous measurement of acetylcholine (ACh). The sensor comprises a small volume prereactor containing immobilized choline oxidase (ChOx) and catalase (or horseradish peroxidase, HRP), a small volume enzymatic reactor containing immobilized acetylcholine esterase (AChE) and ChOx and a glassy carbon electrode modified with Os‐polyvinylpyrridine ‐HRP. The sensor can measure ACh selectively even when the choline (Ch) concentration is more than 1000 times that of ACh, when th ChOx and catalase are immobilized in the prereactor. The sensor shows a wide linear relationship from 5 nM to 1 μM and a detection limit of 5 nM (S/N = 2), which is comparable to the ACh detection limit achieved by liquid chromatography/electrochemistry. It also shows high selectivity against other neurotransmitters and interferents. The properties of the sensor combined with a microdialysis (MD) probe were also studied by measuring a solution containing a much higher concentration of Ch than ACh.</jats:p> On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe Electroanalysis |
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10.1002/elan.1140091209 |
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Wiley, 1997 |
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Wiley, 1997 |
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Wiley |
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Electroanalysis |
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| title |
On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| title_unstemmed |
On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| title_full |
On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| title_fullStr |
On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| title_full_unstemmed |
On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| title_short |
On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| title_sort |
on‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| topic |
Electrochemistry Analytical Chemistry |
| url |
http://dx.doi.org/10.1002/elan.1140091209 |
| publishDate |
1997 |
| physical |
912-916 |
| description |
<jats:title>Abstract</jats:title><jats:p>We have developed an on‐line electrochemical sensor for the continuous measurement of acetylcholine (ACh). The sensor comprises a small volume prereactor containing immobilized choline oxidase (ChOx) and catalase (or horseradish peroxidase, HRP), a small volume enzymatic reactor containing immobilized acetylcholine esterase (AChE) and ChOx and a glassy carbon electrode modified with Os‐polyvinylpyrridine ‐HRP. The sensor can measure ACh selectively even when the choline (Ch) concentration is more than 1000 times that of ACh, when th ChOx and catalase are immobilized in the prereactor. The sensor shows a wide linear relationship from 5 nM to 1 μM and a detection limit of 5 nM (S/N = 2), which is comparable to the ACh detection limit achieved by liquid chromatography/electrochemistry. It also shows high selectivity against other neurotransmitters and interferents. The properties of the sensor combined with a microdialysis (MD) probe were also studied by measuring a solution containing a much higher concentration of Ch than ACh.</jats:p> |
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| author | Horiuchi, Tsutomu, Torimitsu, Keiichi, Yamamoto, Katsunobu, Niwa, Osamu |
| author_facet | Horiuchi, Tsutomu, Torimitsu, Keiichi, Yamamoto, Katsunobu, Niwa, Osamu, Horiuchi, Tsutomu, Torimitsu, Keiichi, Yamamoto, Katsunobu, Niwa, Osamu |
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| description | <jats:title>Abstract</jats:title><jats:p>We have developed an on‐line electrochemical sensor for the continuous measurement of acetylcholine (ACh). The sensor comprises a small volume prereactor containing immobilized choline oxidase (ChOx) and catalase (or horseradish peroxidase, HRP), a small volume enzymatic reactor containing immobilized acetylcholine esterase (AChE) and ChOx and a glassy carbon electrode modified with Os‐polyvinylpyrridine ‐HRP. The sensor can measure ACh selectively even when the choline (Ch) concentration is more than 1000 times that of ACh, when th ChOx and catalase are immobilized in the prereactor. The sensor shows a wide linear relationship from 5 nM to 1 μM and a detection limit of 5 nM (S/N = 2), which is comparable to the ACh detection limit achieved by liquid chromatography/electrochemistry. It also shows high selectivity against other neurotransmitters and interferents. The properties of the sensor combined with a microdialysis (MD) probe were also studied by measuring a solution containing a much higher concentration of Ch than ACh.</jats:p> |
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| physical | 912-916 |
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| spelling | Horiuchi, Tsutomu Torimitsu, Keiichi Yamamoto, Katsunobu Niwa, Osamu 1040-0397 1521-4109 Wiley Electrochemistry Analytical Chemistry http://dx.doi.org/10.1002/elan.1140091209 <jats:title>Abstract</jats:title><jats:p>We have developed an on‐line electrochemical sensor for the continuous measurement of acetylcholine (ACh). The sensor comprises a small volume prereactor containing immobilized choline oxidase (ChOx) and catalase (or horseradish peroxidase, HRP), a small volume enzymatic reactor containing immobilized acetylcholine esterase (AChE) and ChOx and a glassy carbon electrode modified with Os‐polyvinylpyrridine ‐HRP. The sensor can measure ACh selectively even when the choline (Ch) concentration is more than 1000 times that of ACh, when th ChOx and catalase are immobilized in the prereactor. The sensor shows a wide linear relationship from 5 nM to 1 μM and a detection limit of 5 nM (S/N = 2), which is comparable to the ACh detection limit achieved by liquid chromatography/electrochemistry. It also shows high selectivity against other neurotransmitters and interferents. The properties of the sensor combined with a microdialysis (MD) probe were also studied by measuring a solution containing a much higher concentration of Ch than ACh.</jats:p> On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe Electroanalysis |
| spellingShingle | Horiuchi, Tsutomu, Torimitsu, Keiichi, Yamamoto, Katsunobu, Niwa, Osamu, Electroanalysis, On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe, Electrochemistry, Analytical Chemistry |
| title | On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| title_full | On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| title_fullStr | On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| title_full_unstemmed | On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| title_short | On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| title_sort | on‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| title_unstemmed | On‐line flow sensor for measuring acetylcholine combined with microdialysis sampling probe |
| topic | Electrochemistry, Analytical Chemistry |
| url | http://dx.doi.org/10.1002/elan.1140091209 |