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Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo
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| Zeitschriftentitel: | Developmental Dynamics |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Developmental Dynamics, 224, 2002, 2, S. 200-209 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Cerdà, Joan Gründ, Christine Franke, Werner W. Brand, Michael Cerdà, Joan Gründ, Christine Franke, Werner W. Brand, Michael |
|---|---|
| author |
Cerdà, Joan Gründ, Christine Franke, Werner W. Brand, Michael |
| spellingShingle |
Cerdà, Joan Gründ, Christine Franke, Werner W. Brand, Michael Developmental Dynamics Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo Developmental Biology |
| author_sort |
cerdà, joan |
| spelling |
Cerdà, Joan Gründ, Christine Franke, Werner W. Brand, Michael 1058-8388 1097-0177 Wiley Developmental Biology http://dx.doi.org/10.1002/dvdy.10101 <jats:title>Abstract</jats:title><jats:p>During the screening of a zebrafish postsomitogenesis embryo cDNA library, we have identified a cDNA corresponding to a novel type of protein localized to the notochordal sheath‐associated extracellular matrix (ECM) of the embryo. The 4.049‐kb mRNA encodes a predicted polypeptide of 1,207 amino acids (122 kDa, pI 10.50) with a potential signal peptide of 20 amino acids. After the signal peptide, the mature protein consists of 1,187 amino acids (119 kDa, pI 10.46), for which the name “Calymmin” (from Greek χαλνμμα, to envelop, to cover) is proposed. The Calymmin mRNA is highly and transiently expressed by the notochord cells of the embryo from the 10‐ to 12‐somite stage to the pharyngula period (13 and 24 hours postfertilization, respectively), and light and electron microscopical immunolocalization analysis revealed that the protein was specifically localized within a granular and filamentous layer of the ECM compartment surrounding the notochord. In zebrafish <jats:italic>no tail</jats:italic> mutants (<jats:italic>ntl</jats:italic><jats:sup><jats:italic>tc41</jats:italic></jats:sup>), in which the notochord precursor cells are present but fail to differentiate, the Calymmin protein was not detected, confirming the notochord origin of Calymmin. These results indicate that Calymmin is a novel constitutive protein of the ECM compartment associated to the perinotochordal sheath in the zebrafish embryo, which is specifically expressed by the differentiating notochord cells. © 2002 Wiley‐Liss, Inc.</jats:p> Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo Developmental Dynamics |
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10.1002/dvdy.10101 |
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Wiley, 2002 |
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Wiley, 2002 |
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| title |
Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| title_unstemmed |
Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| title_full |
Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| title_fullStr |
Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| title_full_unstemmed |
Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| title_short |
Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| title_sort |
molecular characterization of calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| topic |
Developmental Biology |
| url |
http://dx.doi.org/10.1002/dvdy.10101 |
| publishDate |
2002 |
| physical |
200-209 |
| description |
<jats:title>Abstract</jats:title><jats:p>During the screening of a zebrafish postsomitogenesis embryo cDNA library, we have identified a cDNA corresponding to a novel type of protein localized to the notochordal sheath‐associated extracellular matrix (ECM) of the embryo. The 4.049‐kb mRNA encodes a predicted polypeptide of 1,207 amino acids (122 kDa, pI 10.50) with a potential signal peptide of 20 amino acids. After the signal peptide, the mature protein consists of 1,187 amino acids (119 kDa, pI 10.46), for which the name “Calymmin” (from Greek χαλνμμα, to envelop, to cover) is proposed. The Calymmin mRNA is highly and transiently expressed by the notochord cells of the embryo from the 10‐ to 12‐somite stage to the pharyngula period (13 and 24 hours postfertilization, respectively), and light and electron microscopical immunolocalization analysis revealed that the protein was specifically localized within a granular and filamentous layer of the ECM compartment surrounding the notochord. In zebrafish <jats:italic>no tail</jats:italic> mutants (<jats:italic>ntl</jats:italic><jats:sup><jats:italic>tc41</jats:italic></jats:sup>), in which the notochord precursor cells are present but fail to differentiate, the Calymmin protein was not detected, confirming the notochord origin of Calymmin. These results indicate that Calymmin is a novel constitutive protein of the ECM compartment associated to the perinotochordal sheath in the zebrafish embryo, which is specifically expressed by the differentiating notochord cells. © 2002 Wiley‐Liss, Inc.</jats:p> |
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| author | Cerdà, Joan, Gründ, Christine, Franke, Werner W., Brand, Michael |
| author_facet | Cerdà, Joan, Gründ, Christine, Franke, Werner W., Brand, Michael, Cerdà, Joan, Gründ, Christine, Franke, Werner W., Brand, Michael |
| author_sort | cerdà, joan |
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| description | <jats:title>Abstract</jats:title><jats:p>During the screening of a zebrafish postsomitogenesis embryo cDNA library, we have identified a cDNA corresponding to a novel type of protein localized to the notochordal sheath‐associated extracellular matrix (ECM) of the embryo. The 4.049‐kb mRNA encodes a predicted polypeptide of 1,207 amino acids (122 kDa, pI 10.50) with a potential signal peptide of 20 amino acids. After the signal peptide, the mature protein consists of 1,187 amino acids (119 kDa, pI 10.46), for which the name “Calymmin” (from Greek χαλνμμα, to envelop, to cover) is proposed. The Calymmin mRNA is highly and transiently expressed by the notochord cells of the embryo from the 10‐ to 12‐somite stage to the pharyngula period (13 and 24 hours postfertilization, respectively), and light and electron microscopical immunolocalization analysis revealed that the protein was specifically localized within a granular and filamentous layer of the ECM compartment surrounding the notochord. In zebrafish <jats:italic>no tail</jats:italic> mutants (<jats:italic>ntl</jats:italic><jats:sup><jats:italic>tc41</jats:italic></jats:sup>), in which the notochord precursor cells are present but fail to differentiate, the Calymmin protein was not detected, confirming the notochord origin of Calymmin. These results indicate that Calymmin is a novel constitutive protein of the ECM compartment associated to the perinotochordal sheath in the zebrafish embryo, which is specifically expressed by the differentiating notochord cells. © 2002 Wiley‐Liss, Inc.</jats:p> |
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| institution | DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275 |
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| spelling | Cerdà, Joan Gründ, Christine Franke, Werner W. Brand, Michael 1058-8388 1097-0177 Wiley Developmental Biology http://dx.doi.org/10.1002/dvdy.10101 <jats:title>Abstract</jats:title><jats:p>During the screening of a zebrafish postsomitogenesis embryo cDNA library, we have identified a cDNA corresponding to a novel type of protein localized to the notochordal sheath‐associated extracellular matrix (ECM) of the embryo. The 4.049‐kb mRNA encodes a predicted polypeptide of 1,207 amino acids (122 kDa, pI 10.50) with a potential signal peptide of 20 amino acids. After the signal peptide, the mature protein consists of 1,187 amino acids (119 kDa, pI 10.46), for which the name “Calymmin” (from Greek χαλνμμα, to envelop, to cover) is proposed. The Calymmin mRNA is highly and transiently expressed by the notochord cells of the embryo from the 10‐ to 12‐somite stage to the pharyngula period (13 and 24 hours postfertilization, respectively), and light and electron microscopical immunolocalization analysis revealed that the protein was specifically localized within a granular and filamentous layer of the ECM compartment surrounding the notochord. In zebrafish <jats:italic>no tail</jats:italic> mutants (<jats:italic>ntl</jats:italic><jats:sup><jats:italic>tc41</jats:italic></jats:sup>), in which the notochord precursor cells are present but fail to differentiate, the Calymmin protein was not detected, confirming the notochord origin of Calymmin. These results indicate that Calymmin is a novel constitutive protein of the ECM compartment associated to the perinotochordal sheath in the zebrafish embryo, which is specifically expressed by the differentiating notochord cells. © 2002 Wiley‐Liss, Inc.</jats:p> Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo Developmental Dynamics |
| spellingShingle | Cerdà, Joan, Gründ, Christine, Franke, Werner W., Brand, Michael, Developmental Dynamics, Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo, Developmental Biology |
| title | Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| title_full | Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| title_fullStr | Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| title_full_unstemmed | Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| title_short | Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| title_sort | molecular characterization of calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| title_unstemmed | Molecular characterization of Calymmin, a novel notochord sheath‐associated extracellular matrix protein in the zebrafish embryo |
| topic | Developmental Biology |
| url | http://dx.doi.org/10.1002/dvdy.10101 |