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An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA
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| Zeitschriftentitel: | Cytometry |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Cytometry, 12, 1991, 4, S. 366-372 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Bakker, P. J. M. Stap, J. Tukker, C. J. van Oven, C. H. Veenhof, C. H. N. Aten, J. Bakker, P. J. M. Stap, J. Tukker, C. J. van Oven, C. H. Veenhof, C. H. N. Aten, J. |
|---|---|
| author |
Bakker, P. J. M. Stap, J. Tukker, C. J. van Oven, C. H. Veenhof, C. H. N. Aten, J. |
| spellingShingle |
Bakker, P. J. M. Stap, J. Tukker, C. J. van Oven, C. H. Veenhof, C. H. N. Aten, J. Cytometry An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA Cell Biology Endocrinology Hematology Biophysics Pathology and Forensic Medicine |
| author_sort |
bakker, p. j. m. |
| spelling |
Bakker, P. J. M. Stap, J. Tukker, C. J. van Oven, C. H. Veenhof, C. H. N. Aten, J. 0196-4763 1097-0320 Wiley Cell Biology Endocrinology Hematology Biophysics Pathology and Forensic Medicine http://dx.doi.org/10.1002/cyto.990120412 <jats:title>Abstract</jats:title><jats:p>In this paper we describe an indirect fluorescence double staining procedure for the simultaneous detection of IdUrd and CldUrd in the same cell nucleus. Two commercially available antibodies were selected for this purpose. A rat antiBrdUrd monoclonal antibody from Seralab was found to bind specifically to CldUrd and BrdUrd. A mouse monoclonal anti‐BrdUrd antibody from Becton Dickinson used in a 1:2 dilution binds to all halogenated deoxyuridines but, when the cells were extensively washed with Tris buffer with a high salt concentration, almost no binding to CldUrd was observed. An immunofluorescence procedure was developed, based on these primary antibodies, raised in different species (rat and mouse), in combination with highly purified second antibodies: FITC conjugated goat antirat and Texas‐Red conjugated goat antimouse.</jats:p> An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA Cytometry |
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Wiley, 1991 |
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| title |
An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA |
| title_unstemmed |
An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA |
| title_full |
An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA |
| title_fullStr |
An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA |
| title_full_unstemmed |
An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA |
| title_short |
An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA |
| title_sort |
an indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into dna |
| topic |
Cell Biology Endocrinology Hematology Biophysics Pathology and Forensic Medicine |
| url |
http://dx.doi.org/10.1002/cyto.990120412 |
| publishDate |
1991 |
| physical |
366-372 |
| description |
<jats:title>Abstract</jats:title><jats:p>In this paper we describe an indirect fluorescence double staining procedure for the simultaneous detection of IdUrd and CldUrd in the same cell nucleus. Two commercially available antibodies were selected for this purpose. A rat antiBrdUrd monoclonal antibody from Seralab was found to bind specifically to CldUrd and BrdUrd. A mouse monoclonal anti‐BrdUrd antibody from Becton Dickinson used in a 1:2 dilution binds to all halogenated deoxyuridines but, when the cells were extensively washed with Tris buffer with a high salt concentration, almost no binding to CldUrd was observed. An immunofluorescence procedure was developed, based on these primary antibodies, raised in different species (rat and mouse), in combination with highly purified second antibodies: FITC conjugated goat antirat and Texas‐Red conjugated goat antimouse.</jats:p> |
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| author | Bakker, P. J. M., Stap, J., Tukker, C. J., van Oven, C. H., Veenhof, C. H. N., Aten, J. |
| author_facet | Bakker, P. J. M., Stap, J., Tukker, C. J., van Oven, C. H., Veenhof, C. H. N., Aten, J., Bakker, P. J. M., Stap, J., Tukker, C. J., van Oven, C. H., Veenhof, C. H. N., Aten, J. |
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| description | <jats:title>Abstract</jats:title><jats:p>In this paper we describe an indirect fluorescence double staining procedure for the simultaneous detection of IdUrd and CldUrd in the same cell nucleus. Two commercially available antibodies were selected for this purpose. A rat antiBrdUrd monoclonal antibody from Seralab was found to bind specifically to CldUrd and BrdUrd. A mouse monoclonal anti‐BrdUrd antibody from Becton Dickinson used in a 1:2 dilution binds to all halogenated deoxyuridines but, when the cells were extensively washed with Tris buffer with a high salt concentration, almost no binding to CldUrd was observed. An immunofluorescence procedure was developed, based on these primary antibodies, raised in different species (rat and mouse), in combination with highly purified second antibodies: FITC conjugated goat antirat and Texas‐Red conjugated goat antimouse.</jats:p> |
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| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9jeXRvLjk5MDEyMDQxMg |
| imprint | Wiley, 1991 |
| imprint_str_mv | Wiley, 1991 |
| institution | DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
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| physical | 366-372 |
| publishDate | 1991 |
| publishDateSort | 1991 |
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| series | Cytometry |
| source_id | 49 |
| spelling | Bakker, P. J. M. Stap, J. Tukker, C. J. van Oven, C. H. Veenhof, C. H. N. Aten, J. 0196-4763 1097-0320 Wiley Cell Biology Endocrinology Hematology Biophysics Pathology and Forensic Medicine http://dx.doi.org/10.1002/cyto.990120412 <jats:title>Abstract</jats:title><jats:p>In this paper we describe an indirect fluorescence double staining procedure for the simultaneous detection of IdUrd and CldUrd in the same cell nucleus. Two commercially available antibodies were selected for this purpose. A rat antiBrdUrd monoclonal antibody from Seralab was found to bind specifically to CldUrd and BrdUrd. A mouse monoclonal anti‐BrdUrd antibody from Becton Dickinson used in a 1:2 dilution binds to all halogenated deoxyuridines but, when the cells were extensively washed with Tris buffer with a high salt concentration, almost no binding to CldUrd was observed. An immunofluorescence procedure was developed, based on these primary antibodies, raised in different species (rat and mouse), in combination with highly purified second antibodies: FITC conjugated goat antirat and Texas‐Red conjugated goat antimouse.</jats:p> An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA Cytometry |
| spellingShingle | Bakker, P. J. M., Stap, J., Tukker, C. J., van Oven, C. H., Veenhof, C. H. N., Aten, J., Cytometry, An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA, Cell Biology, Endocrinology, Hematology, Biophysics, Pathology and Forensic Medicine |
| title | An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA |
| title_full | An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA |
| title_fullStr | An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA |
| title_full_unstemmed | An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA |
| title_short | An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA |
| title_sort | an indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into dna |
| title_unstemmed | An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA |
| topic | Cell Biology, Endocrinology, Hematology, Biophysics, Pathology and Forensic Medicine |
| url | http://dx.doi.org/10.1002/cyto.990120412 |