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Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats
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| Zeitschriftentitel: | Cytometry |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Cytometry, 7, 1986, 1, S. 64-69 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Jacobs, R. M. Boyce, J. T. Kociba, G. J. Jacobs, R. M. Boyce, J. T. Kociba, G. J. |
|---|---|
| author |
Jacobs, R. M. Boyce, J. T. Kociba, G. J. |
| spellingShingle |
Jacobs, R. M. Boyce, J. T. Kociba, G. J. Cytometry Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats Cell Biology Endocrinology Hematology Biophysics Pathology and Forensic Medicine |
| author_sort |
jacobs, r. m. |
| spelling |
Jacobs, R. M. Boyce, J. T. Kociba, G. J. 0196-4763 1097-0320 Wiley Cell Biology Endocrinology Hematology Biophysics Pathology and Forensic Medicine http://dx.doi.org/10.1002/cyto.990070109 <jats:title>Abstract</jats:title><jats:p>This study demonstrates the potential usefulness of a flow cytometric technique to measure platelet survival time in cats utilizing autologous platelets labeled in vitro with fluorescein isothiocyanate (FITC). When compared with a <jats:sup>51</jats:sup>Cr method, no significant differences in estimated survival times were found. Both the <jats:sup>51</jats:sup>Cr and FITC‐labeling procedures induced similar changes in platelet shape and collagen‐induced aggregation. Platelets labeled with FITC had significantly greater volumes compared with those of glutaraldehyde‐fixed platelets. These changes were primarily related to the platelet centrifugation and washing procedures rather than the labels themselves. This novel technique potentially has wide applicability to cell circulation time studies as flow cytometry equipment becomes more readily available.</jats:p><jats:p>In a preliminary study of the platelet survival time in feline leukemia virus (FeLV)‐infected cats, two of three cats had significantly reduced survival times using both flow cytometric and radioisotopic methods. These data suggest increased platelet turnover in FeLV‐infected cats.</jats:p> Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats Cytometry |
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10.1002/cyto.990070109 |
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Wiley, 1986 |
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| title |
Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| title_unstemmed |
Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| title_full |
Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| title_fullStr |
Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| title_full_unstemmed |
Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| title_short |
Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| title_sort |
flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| topic |
Cell Biology Endocrinology Hematology Biophysics Pathology and Forensic Medicine |
| url |
http://dx.doi.org/10.1002/cyto.990070109 |
| publishDate |
1986 |
| physical |
64-69 |
| description |
<jats:title>Abstract</jats:title><jats:p>This study demonstrates the potential usefulness of a flow cytometric technique to measure platelet survival time in cats utilizing autologous platelets labeled in vitro with fluorescein isothiocyanate (FITC). When compared with a <jats:sup>51</jats:sup>Cr method, no significant differences in estimated survival times were found. Both the <jats:sup>51</jats:sup>Cr and FITC‐labeling procedures induced similar changes in platelet shape and collagen‐induced aggregation. Platelets labeled with FITC had significantly greater volumes compared with those of glutaraldehyde‐fixed platelets. These changes were primarily related to the platelet centrifugation and washing procedures rather than the labels themselves. This novel technique potentially has wide applicability to cell circulation time studies as flow cytometry equipment becomes more readily available.</jats:p><jats:p>In a preliminary study of the platelet survival time in feline leukemia virus (FeLV)‐infected cats, two of three cats had significantly reduced survival times using both flow cytometric and radioisotopic methods. These data suggest increased platelet turnover in FeLV‐infected cats.</jats:p> |
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| author | Jacobs, R. M., Boyce, J. T., Kociba, G. J. |
| author_facet | Jacobs, R. M., Boyce, J. T., Kociba, G. J., Jacobs, R. M., Boyce, J. T., Kociba, G. J. |
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| description | <jats:title>Abstract</jats:title><jats:p>This study demonstrates the potential usefulness of a flow cytometric technique to measure platelet survival time in cats utilizing autologous platelets labeled in vitro with fluorescein isothiocyanate (FITC). When compared with a <jats:sup>51</jats:sup>Cr method, no significant differences in estimated survival times were found. Both the <jats:sup>51</jats:sup>Cr and FITC‐labeling procedures induced similar changes in platelet shape and collagen‐induced aggregation. Platelets labeled with FITC had significantly greater volumes compared with those of glutaraldehyde‐fixed platelets. These changes were primarily related to the platelet centrifugation and washing procedures rather than the labels themselves. This novel technique potentially has wide applicability to cell circulation time studies as flow cytometry equipment becomes more readily available.</jats:p><jats:p>In a preliminary study of the platelet survival time in feline leukemia virus (FeLV)‐infected cats, two of three cats had significantly reduced survival times using both flow cytometric and radioisotopic methods. These data suggest increased platelet turnover in FeLV‐infected cats.</jats:p> |
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| imprint | Wiley, 1986 |
| imprint_str_mv | Wiley, 1986 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| spelling | Jacobs, R. M. Boyce, J. T. Kociba, G. J. 0196-4763 1097-0320 Wiley Cell Biology Endocrinology Hematology Biophysics Pathology and Forensic Medicine http://dx.doi.org/10.1002/cyto.990070109 <jats:title>Abstract</jats:title><jats:p>This study demonstrates the potential usefulness of a flow cytometric technique to measure platelet survival time in cats utilizing autologous platelets labeled in vitro with fluorescein isothiocyanate (FITC). When compared with a <jats:sup>51</jats:sup>Cr method, no significant differences in estimated survival times were found. Both the <jats:sup>51</jats:sup>Cr and FITC‐labeling procedures induced similar changes in platelet shape and collagen‐induced aggregation. Platelets labeled with FITC had significantly greater volumes compared with those of glutaraldehyde‐fixed platelets. These changes were primarily related to the platelet centrifugation and washing procedures rather than the labels themselves. This novel technique potentially has wide applicability to cell circulation time studies as flow cytometry equipment becomes more readily available.</jats:p><jats:p>In a preliminary study of the platelet survival time in feline leukemia virus (FeLV)‐infected cats, two of three cats had significantly reduced survival times using both flow cytometric and radioisotopic methods. These data suggest increased platelet turnover in FeLV‐infected cats.</jats:p> Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats Cytometry |
| spellingShingle | Jacobs, R. M., Boyce, J. T., Kociba, G. J., Cytometry, Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats, Cell Biology, Endocrinology, Hematology, Biophysics, Pathology and Forensic Medicine |
| title | Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| title_full | Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| title_fullStr | Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| title_full_unstemmed | Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| title_short | Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| title_sort | flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| title_unstemmed | Flow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats |
| topic | Cell Biology, Endocrinology, Hematology, Biophysics, Pathology and Forensic Medicine |
| url | http://dx.doi.org/10.1002/cyto.990070109 |