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Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors
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| Zeitschriftentitel: | Chemical Engineering & Technology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Chemical Engineering & Technology, 42, 2019, 10, S. 2009-2017 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Peschke, Theo Bitterwolf, Patrick Rabe, Kersten S. Niemeyer, Christof M. Peschke, Theo Bitterwolf, Patrick Rabe, Kersten S. Niemeyer, Christof M. |
|---|---|
| author |
Peschke, Theo Bitterwolf, Patrick Rabe, Kersten S. Niemeyer, Christof M. |
| spellingShingle |
Peschke, Theo Bitterwolf, Patrick Rabe, Kersten S. Niemeyer, Christof M. Chemical Engineering & Technology Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors Industrial and Manufacturing Engineering General Chemical Engineering General Chemistry |
| author_sort |
peschke, theo |
| spelling |
Peschke, Theo Bitterwolf, Patrick Rabe, Kersten S. Niemeyer, Christof M. 0930-7516 1521-4125 Wiley Industrial and Manufacturing Engineering General Chemical Engineering General Chemistry http://dx.doi.org/10.1002/ceat.201900073 <jats:title>Abstract</jats:title><jats:p>The industrial implementation of enzymes in flow biocatalysis microreactors is expected to be essential for the emergence of a bio‐based circular economy. Major challenges concern the efficient immobilization of delicate enzymes inside miniaturized reactors without compromising their catalytic activity. We describe the exploitation of the widely used His‐tag system in a microfluidic packed‐bed reactor that contains ketoreductase‐functionalized magnetic beads. In a continuous process, these reactors produced highly stereoselective (<jats:italic>R</jats:italic>)‐configured alcohols (d.r. 99:1) with an average conversion of > 90 % for more than 4 days. We believe that such miniaturized flow reactors can be of great utility for future sustainable production processes.</jats:p> Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors Chemical Engineering & Technology |
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| title |
Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors |
| title_unstemmed |
Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors |
| title_full |
Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors |
| title_fullStr |
Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors |
| title_full_unstemmed |
Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors |
| title_short |
Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors |
| title_sort |
self‐immobilizing oxidoreductases for flow biocatalysis in miniaturized packed‐bed reactors |
| topic |
Industrial and Manufacturing Engineering General Chemical Engineering General Chemistry |
| url |
http://dx.doi.org/10.1002/ceat.201900073 |
| publishDate |
2019 |
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2009-2017 |
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<jats:title>Abstract</jats:title><jats:p>The industrial implementation of enzymes in flow biocatalysis microreactors is expected to be essential for the emergence of a bio‐based circular economy. Major challenges concern the efficient immobilization of delicate enzymes inside miniaturized reactors without compromising their catalytic activity. We describe the exploitation of the widely used His‐tag system in a microfluidic packed‐bed reactor that contains ketoreductase‐functionalized magnetic beads. In a continuous process, these reactors produced highly stereoselective (<jats:italic>R</jats:italic>)‐configured alcohols (d.r. 99:1) with an average conversion of > 90 % for more than 4 days. We believe that such miniaturized flow reactors can be of great utility for future sustainable production processes.</jats:p> |
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| author | Peschke, Theo, Bitterwolf, Patrick, Rabe, Kersten S., Niemeyer, Christof M. |
| author_facet | Peschke, Theo, Bitterwolf, Patrick, Rabe, Kersten S., Niemeyer, Christof M., Peschke, Theo, Bitterwolf, Patrick, Rabe, Kersten S., Niemeyer, Christof M. |
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| description | <jats:title>Abstract</jats:title><jats:p>The industrial implementation of enzymes in flow biocatalysis microreactors is expected to be essential for the emergence of a bio‐based circular economy. Major challenges concern the efficient immobilization of delicate enzymes inside miniaturized reactors without compromising their catalytic activity. We describe the exploitation of the widely used His‐tag system in a microfluidic packed‐bed reactor that contains ketoreductase‐functionalized magnetic beads. In a continuous process, these reactors produced highly stereoselective (<jats:italic>R</jats:italic>)‐configured alcohols (d.r. 99:1) with an average conversion of > 90 % for more than 4 days. We believe that such miniaturized flow reactors can be of great utility for future sustainable production processes.</jats:p> |
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| physical | 2009-2017 |
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| series | Chemical Engineering & Technology |
| source_id | 49 |
| spelling | Peschke, Theo Bitterwolf, Patrick Rabe, Kersten S. Niemeyer, Christof M. 0930-7516 1521-4125 Wiley Industrial and Manufacturing Engineering General Chemical Engineering General Chemistry http://dx.doi.org/10.1002/ceat.201900073 <jats:title>Abstract</jats:title><jats:p>The industrial implementation of enzymes in flow biocatalysis microreactors is expected to be essential for the emergence of a bio‐based circular economy. Major challenges concern the efficient immobilization of delicate enzymes inside miniaturized reactors without compromising their catalytic activity. We describe the exploitation of the widely used His‐tag system in a microfluidic packed‐bed reactor that contains ketoreductase‐functionalized magnetic beads. In a continuous process, these reactors produced highly stereoselective (<jats:italic>R</jats:italic>)‐configured alcohols (d.r. 99:1) with an average conversion of > 90 % for more than 4 days. We believe that such miniaturized flow reactors can be of great utility for future sustainable production processes.</jats:p> Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors Chemical Engineering & Technology |
| spellingShingle | Peschke, Theo, Bitterwolf, Patrick, Rabe, Kersten S., Niemeyer, Christof M., Chemical Engineering & Technology, Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors, Industrial and Manufacturing Engineering, General Chemical Engineering, General Chemistry |
| title | Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors |
| title_full | Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors |
| title_fullStr | Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors |
| title_full_unstemmed | Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors |
| title_short | Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors |
| title_sort | self‐immobilizing oxidoreductases for flow biocatalysis in miniaturized packed‐bed reactors |
| title_unstemmed | Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors |
| topic | Industrial and Manufacturing Engineering, General Chemical Engineering, General Chemistry |
| url | http://dx.doi.org/10.1002/ceat.201900073 |