Eintrag weiter verarbeiten
Comparison of antibody functionality using different immobilization methods
Gespeichert in:
| Zeitschriftentitel: | Biotechnology and Bioengineering |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Biotechnology and Bioengineering, 84, 2003, 2, S. 215-223 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Danczyk, R. Krieder, B. North, A. Webster, T. HogenEsch, H. Rundell, A. Danczyk, R. Krieder, B. North, A. Webster, T. HogenEsch, H. Rundell, A. |
|---|---|
| author |
Danczyk, R. Krieder, B. North, A. Webster, T. HogenEsch, H. Rundell, A. |
| spellingShingle |
Danczyk, R. Krieder, B. North, A. Webster, T. HogenEsch, H. Rundell, A. Biotechnology and Bioengineering Comparison of antibody functionality using different immobilization methods Applied Microbiology and Biotechnology Bioengineering Biotechnology |
| author_sort |
danczyk, r. |
| spelling |
Danczyk, R. Krieder, B. North, A. Webster, T. HogenEsch, H. Rundell, A. 0006-3592 1097-0290 Wiley Applied Microbiology and Biotechnology Bioengineering Biotechnology http://dx.doi.org/10.1002/bit.10760 <jats:title>Abstract</jats:title><jats:p>This study investigates the influence of antibody immobilization methods on antigen capture. Adsorption and two surface chemistries, an aminosilane chemistry and a common heterobifunctional crosslinker (N‐gamma‐maleimidobutyryloxy‐succinimide ester, GMBS), were compared and evaluated for their ability to immobilize antibodies and capture antigen. The role of protein A as an orienting protein scaffold component in each of these techniques was also evaluated. Through experimentation it was determined that the GMBS technique immobilized the highest amount of antibody and minimized nonspecific binding. For all techniques, the most functional antibodies were found to be those immobilized with protein A. Interestingly, the aminosilane technique demonstrated the highest antigen capture with antibody alone but also exhibited the highest level of nonspecific binding. © 2003 Wiley Periodicals. Biotechnol Bioeng 84: 215–223, 2003.</jats:p> Comparison of antibody functionality using different immobilization methods Biotechnology and Bioengineering |
| doi_str_mv |
10.1002/bit.10760 |
| facet_avail |
Online |
| finc_class_facet |
Biologie Technik Chemie und Pharmazie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9iaXQuMTA3NjA |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9iaXQuMTA3NjA |
| institution |
DE-Brt1 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 |
| imprint |
Wiley, 2003 |
| imprint_str_mv |
Wiley, 2003 |
| issn |
0006-3592 1097-0290 |
| issn_str_mv |
0006-3592 1097-0290 |
| language |
English |
| mega_collection |
Wiley (CrossRef) |
| match_str |
danczyk2003comparisonofantibodyfunctionalityusingdifferentimmobilizationmethods |
| publishDateSort |
2003 |
| publisher |
Wiley |
| recordtype |
ai |
| record_format |
ai |
| series |
Biotechnology and Bioengineering |
| source_id |
49 |
| title |
Comparison of antibody functionality using different immobilization methods |
| title_unstemmed |
Comparison of antibody functionality using different immobilization methods |
| title_full |
Comparison of antibody functionality using different immobilization methods |
| title_fullStr |
Comparison of antibody functionality using different immobilization methods |
| title_full_unstemmed |
Comparison of antibody functionality using different immobilization methods |
| title_short |
Comparison of antibody functionality using different immobilization methods |
| title_sort |
comparison of antibody functionality using different immobilization methods |
| topic |
Applied Microbiology and Biotechnology Bioengineering Biotechnology |
| url |
http://dx.doi.org/10.1002/bit.10760 |
| publishDate |
2003 |
| physical |
215-223 |
| description |
<jats:title>Abstract</jats:title><jats:p>This study investigates the influence of antibody immobilization methods on antigen capture. Adsorption and two surface chemistries, an aminosilane chemistry and a common heterobifunctional crosslinker (N‐gamma‐maleimidobutyryloxy‐succinimide ester, GMBS), were compared and evaluated for their ability to immobilize antibodies and capture antigen. The role of protein A as an orienting protein scaffold component in each of these techniques was also evaluated. Through experimentation it was determined that the GMBS technique immobilized the highest amount of antibody and minimized nonspecific binding. For all techniques, the most functional antibodies were found to be those immobilized with protein A. Interestingly, the aminosilane technique demonstrated the highest antigen capture with antibody alone but also exhibited the highest level of nonspecific binding. © 2003 Wiley Periodicals. Biotechnol Bioeng 84: 215–223, 2003.</jats:p> |
| container_issue |
2 |
| container_start_page |
215 |
| container_title |
Biotechnology and Bioengineering |
| container_volume |
84 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792347962140000258 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T18:03:35.475Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Comparison+of+antibody+functionality+using+different+immobilization+methods&rft.date=2003-10-20&genre=article&issn=1097-0290&volume=84&issue=2&spage=215&epage=223&pages=215-223&jtitle=Biotechnology+and+Bioengineering&atitle=Comparison+of+antibody+functionality+using+different+immobilization+methods&aulast=Rundell&aufirst=A.&rft_id=info%3Adoi%2F10.1002%2Fbit.10760&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792347962140000258 |
| author | Danczyk, R., Krieder, B., North, A., Webster, T., HogenEsch, H., Rundell, A. |
| author_facet | Danczyk, R., Krieder, B., North, A., Webster, T., HogenEsch, H., Rundell, A., Danczyk, R., Krieder, B., North, A., Webster, T., HogenEsch, H., Rundell, A. |
| author_sort | danczyk, r. |
| container_issue | 2 |
| container_start_page | 215 |
| container_title | Biotechnology and Bioengineering |
| container_volume | 84 |
| description | <jats:title>Abstract</jats:title><jats:p>This study investigates the influence of antibody immobilization methods on antigen capture. Adsorption and two surface chemistries, an aminosilane chemistry and a common heterobifunctional crosslinker (N‐gamma‐maleimidobutyryloxy‐succinimide ester, GMBS), were compared and evaluated for their ability to immobilize antibodies and capture antigen. The role of protein A as an orienting protein scaffold component in each of these techniques was also evaluated. Through experimentation it was determined that the GMBS technique immobilized the highest amount of antibody and minimized nonspecific binding. For all techniques, the most functional antibodies were found to be those immobilized with protein A. Interestingly, the aminosilane technique demonstrated the highest antigen capture with antibody alone but also exhibited the highest level of nonspecific binding. © 2003 Wiley Periodicals. Biotechnol Bioeng 84: 215–223, 2003.</jats:p> |
| doi_str_mv | 10.1002/bit.10760 |
| facet_avail | Online |
| finc_class_facet | Biologie, Technik, Chemie und Pharmazie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9iaXQuMTA3NjA |
| imprint | Wiley, 2003 |
| imprint_str_mv | Wiley, 2003 |
| institution | DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
| issn | 0006-3592, 1097-0290 |
| issn_str_mv | 0006-3592, 1097-0290 |
| language | English |
| last_indexed | 2024-03-01T18:03:35.475Z |
| match_str | danczyk2003comparisonofantibodyfunctionalityusingdifferentimmobilizationmethods |
| mega_collection | Wiley (CrossRef) |
| physical | 215-223 |
| publishDate | 2003 |
| publishDateSort | 2003 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | Biotechnology and Bioengineering |
| source_id | 49 |
| spelling | Danczyk, R. Krieder, B. North, A. Webster, T. HogenEsch, H. Rundell, A. 0006-3592 1097-0290 Wiley Applied Microbiology and Biotechnology Bioengineering Biotechnology http://dx.doi.org/10.1002/bit.10760 <jats:title>Abstract</jats:title><jats:p>This study investigates the influence of antibody immobilization methods on antigen capture. Adsorption and two surface chemistries, an aminosilane chemistry and a common heterobifunctional crosslinker (N‐gamma‐maleimidobutyryloxy‐succinimide ester, GMBS), were compared and evaluated for their ability to immobilize antibodies and capture antigen. The role of protein A as an orienting protein scaffold component in each of these techniques was also evaluated. Through experimentation it was determined that the GMBS technique immobilized the highest amount of antibody and minimized nonspecific binding. For all techniques, the most functional antibodies were found to be those immobilized with protein A. Interestingly, the aminosilane technique demonstrated the highest antigen capture with antibody alone but also exhibited the highest level of nonspecific binding. © 2003 Wiley Periodicals. Biotechnol Bioeng 84: 215–223, 2003.</jats:p> Comparison of antibody functionality using different immobilization methods Biotechnology and Bioengineering |
| spellingShingle | Danczyk, R., Krieder, B., North, A., Webster, T., HogenEsch, H., Rundell, A., Biotechnology and Bioengineering, Comparison of antibody functionality using different immobilization methods, Applied Microbiology and Biotechnology, Bioengineering, Biotechnology |
| title | Comparison of antibody functionality using different immobilization methods |
| title_full | Comparison of antibody functionality using different immobilization methods |
| title_fullStr | Comparison of antibody functionality using different immobilization methods |
| title_full_unstemmed | Comparison of antibody functionality using different immobilization methods |
| title_short | Comparison of antibody functionality using different immobilization methods |
| title_sort | comparison of antibody functionality using different immobilization methods |
| title_unstemmed | Comparison of antibody functionality using different immobilization methods |
| topic | Applied Microbiology and Biotechnology, Bioengineering, Biotechnology |
| url | http://dx.doi.org/10.1002/bit.10760 |